• Title/Summary/Keyword: Lee Sam

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Studies on the Yield, Ecological and Morphological Characteristics of Several Strains of Lentinus edodes (표고의 각(各) 계통별(系統別) 발생양(發生量)과 생태적(生態的) 및 형태적(形態的) 특징(特徵)에 관한 연구(硏究))

  • Lee, Eung-Rae;Hwang, Kye-Seong;Lee, Joon-Sam
    • The Korean Journal of Mycology
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    • v.8 no.1
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    • pp.33-43
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    • 1980
  • Thirteen strains or crosses of Lentinus edodes obtained from home and abroad were compared for their mycelial characteristics on artificial media. Eleven of them were selected and inoculated on logs in order to study for their productivity, and the morphological characteristics of the mushrooms were investigated. 1) Seven strains, i. e., 11001, 12001, 13005, 13012, 13015, 12004 and 20101 were grown well on the sawdust medium. 2) The best mycelial growth and establish­ment of infection on logs were obtained from strains 20101 and 11001. 3) The highest yield of the mushrooms on logs during the six year periods were obtained from strains 11001, 12001 and 20101. 4) The highest yield of the mushrooms usually was obtained on the fourth year after inoculation. 5) The fruit body productivity in terms of dry weight recovery was the highest in strains 13005 and 20101. 6) The most productive period of the mushrooms during the years depended on strains. Most prevalent types of the strains were summer and autumn growing types, and more mushrooms were produced during these periods. 7) Morphological characteristics of the mushroom were more or less dependent on environmental conditions. 8) Thicker fruit bodies and relatively uniform shapes of the mushroom were produced by strain 20101.

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The Effect of 12-weeks Aquatic Exercise on Dynamic Balance and Pain Factors in Elderly Osteoarthritis Patients (12주간의 수중운동이 노인 골관절염 환자의 동적평형성과 통증요인에 미치는 영향)

  • Im, Sun-Young;Eo, Su-Ju;Kim, Song-June;Hur, Sung-Hoon;An, Kyung-Jun;Lee, Jang-Kyu;Lee, Jong-Sam
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.2
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    • pp.601-609
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    • 2016
  • This study evaluated the dynamic balance and pain after 12 weeks aquatic exercise in old osteoarthritis patients. The dynamic balances were assessed using 2 different experimental conditions (Open Eye Condition; OEC, Closed Eye Condition; CEC). Twenty-two subjects were assigned one of the two experimental groups: arthritis group (ART, age=$65.25{\pm}5.9$, n=11), control group(CON, age=$64.50{\pm}7$, n=11). All subjects participated in the aquatic exercise program three times a week for 12 weeks. The dynamic balances (OEC (p<.001), CEC (p<.05)) capability were improved significantly in the ART group after 12 week aquatic exercise. Visual Analogue Scale (VAS) was decreased significantly [Factor 10(Soft chair, p<0.01), 11 (Lying down, p<0.05), 12(Handicap, p<0.05), 13 (Work interference, p<0.01)] in the ART group. In conclusion, aquatic exercise was effective in improving the overall health status and the capacity of dynamic balances and reducing the degree of pain in osteoarthritis patients.

Zooplankton Community and Distributions of Copepods in Relation to Eutrophic Evaluation in Chinhae Bay (진해만 수질 환경과 동물플랑크톤 군집 및 요각류 분포 특성)

  • KANG Young-Shil;PARK Joo-Suck;LEE Sam-Seuk;KIM Hak-Gyoon;LEE Phil-Yong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.29 no.4
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    • pp.415-430
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    • 1996
  • Spatio-temporal variations in zooplankton community and ropepod indicator species were investigated along with the interaction between zooplankton distribution and environmental factors in Chinhae Bay. Zooplankton samples were monthly collected at 7 stations from February to September in 1993. A NORPAC net was vertically hauled from bottom to surface, At the same station, environmental factors such as temperature, salinity and COD (chemical oxygen demand) were measured at two different water layers, surface and bottom. In August and September, salinity declined below 30.00‰ , while eutrophic parameters such as COD showed the higher concentrations than those in other months, with higher concentrations at inner bay stations. Salinities were, however, higher at bay mouth areas. These distributional patterns were believed to be caused by input and dispersion of organic matters from nearby land. Zooplankton communities were composed of 7~14. Of these, Noctiluca scintillans was predominant and occupied 90.6‰ of total zooplankton abundance. Cladocera and Copepoda were secondly abundant taxa. Among 6 to 10 copepod species appeared, Acartia omorii and A. hudsonics were most common species during the survey months except March and September. Species diversities were greater, in general, at inner bay than outer bay. The lowest diversity index was observed in February, while the highest in July. Cluster analysis could divide the study area into 2 or 4 zones for each month. Zone 1, mouth area of the bay, was characterized by the influence of offshore waters. Zone II was mixing area. Zone III and IV seemed to be affected by nearby land.

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Treatment of Nightsoil Sludge by Vermicomposting (Vermicomposting에 의한 분뇨슬러지의 처리)

  • Lee, Ju-Sam;Lee, Moo-Choon
    • Journal of the Korea Organic Resources Recycling Association
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    • v.4 no.2
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    • pp.35-45
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    • 1996
  • In order to investigate the possibility of treating nightsoil sludge, the growth trend experiments with vermicompsting was performed and the stability of worm castings was evaluated by the analysis of minerals according to growth periods. Survival rate(SR) of earthworms decreased as earthworms grow up to 7 weeks. The growth period of the earthworms could be classified into two phases on the basis of the ratios of cast to feed ingested(ID). The growth period of the earthworms up to 3 weeks, the high percentage of dry weight of feed ingested(ID) is mainly utiliz-ed to an increase in fresh weight of earthworms, and after the 3 weeks, it is utilized to cast production in this experiment, respectively. The highest values of increasing rate(IR) and biomass of the earthworms(BE) were obtained at 3 weeks and those were 10.59mg/day and 14.48g, respectively. Between the increasing rate(IR) and biomass of the earthworms(BE), a highly positive correlation coefficient(p<0.001) ap-peared. The highest values of biomass of the earthworms(BE) and cast production(CW) were obtained at 3 and 7 weeks, respectively. All the concentrations of minerals except calcium(Ca) in worm castings was lower than the values in nightsoil sludge. It was considered that the major portion of minerals in nightsoil sludge may have been retained in the bodies of earthworms. And these values were lower than the regulated levels in organic fertilizers that the regula-tion standards. In conclusion, the worm castings are very stable for the use as soil conditioner or organic fertilizers.

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Heavy Metal Adsorption Capacity of Zoogloea ramigera 115 and Zoogloea ramigera l15SLR. (Zoogloea ramigera 115와 Zoogloea ramigera l15SLR의 중금속 흡착능 비교)

  • Lee, Han-Ki;Bae, Woo-Chul;Jin, Wook;Jung, Wook-Jin;Lee, Sam-Pin;Jeong, Byeong-Chul
    • Microbiology and Biotechnology Letters
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    • v.26 no.1
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    • pp.83-88
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    • 1998
  • Heavy metal removal by Z. ramigera 115 and soluble slime polymer producing mutant Z. ramigera 115SLR was investigated. Both strains showed similar tolerance against $Cd^{2+}$, $Co^{2+}$, $Cu^{2+}$, $Ni^{2+}$ and $Fe^{2+}$. When cells were cultivated in the presence of 500 ppm $Cd^{2+}$, the mutant strain removed 1.5 fold more metal than the wild type did at same biomass. Metal adsorption capacities were in the order of Z. ramigera l15SLR polymer > Z. ramigera 115 polymer > Z. ramigera 115 cell >Z. ramigera l15SLR cell. The optimum pH for metal adsorption was 7.5. Langmuir and Freundlich isotherms indicated that Qmax and 1/n of Z. ramigera l15SLR polymer were 164.2 mg $Cd^{2+}$/g dw and 0.496, respectively. These results showed that the polymer of Z. ramigera l15SLR could be used as an effective metal adsorbate.

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In Vitro Assessment of MRI Safety at 1.5 T and 3.0 T for Bone-Anchored Hearing Aid Implant (Bone-Anchored Hearing Aid Implant에 대한 1.5 T와 3.0 T에서 MRI 안전성의 생체외 평가)

  • Yeon, Kyoo-Jin;Kim, Hyun-Soo;Lee, Seung-keun;Lee, Tae-Soo
    • Journal of radiological science and technology
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    • v.40 no.1
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    • pp.19-25
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    • 2017
  • The aim of this study was to evaluate Magnetic Resonance Imaging safety by measuring the translational attraction, torque and susceptibility artifact for Bone-Anchored Hearing Aid (BAHA) implant at 1.5 T and 3.0 T MRI by standard criteria. In vitro assessment tools were made of acrylic-resin by American Society for Testing and Materials (ASTM) F2052-06 and F2119-07 standard. Translational attraction of BAHA implant was measured by the maximum deflection angle at 96 cm position, where the magnetically induced deflection was the greatest. The torque was assessed by the qualitative criteria of evaluating the alignment and rotation pattern, when the BAHA implant was positioned on a line with $45^{\circ}$ intervals inside the circular container in the center of the bore. The susceptibility artifact images were obtained using the hanged test tool, which was filled with $CuSO_4$ solution. And then the artifact size was measured using Susceptibility A rtifact Measurement (SA M) software. In results, the translational attraction was 0 mm at both 1.5 T and 3.0 T and the torque was 0(no torque) at 1.5 T, and +1(mild torque) at 3.0 T. The size of susceptibility artifacts was between 13.20 mm and 38.91 mm. Therefore, The BAHA implant was safe for the patient in clinical MR environment.

Genetic Authentication of Cynanchi Wilfordii Radix and Cynanchi Auriculati Radix by Using Conventional-PCR and Real-time PCR (Conventional-PCR 및 Real-time PCR을 이용한 백수오와 이엽우피소의 유전자 종감별 시험법 비교)

  • Ryu, Hoe Jin;Kim, Ae Kyung;Kim, Sung Dan;Jung, Sam Joo;Jang, Jung Im;Lee, Hee Jin;Lee, Jung Mi;Yu, In Sil;Jung, Kweon
    • Korean Journal of Pharmacognosy
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    • v.49 no.1
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    • pp.55-64
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    • 2018
  • Recently, it has been a big issue to distinguish the dried roots of Cynanchum wilfordii and C. auriculatum in health functional food market. The original plant species of Cynanchi Wilfordii Radix belong to the Asclepiadaceae family is differentially described in the national pharmacopoeia of Korea, China and Japan. Owing to the morphological similarities of the dried roots of this plant to those of C. auriculatum, which is often misidentified in Korean herbal medicine marketplace, distinguishing these two species is exceedingly difficult. The purpose of this study was to compare the conventional-PCR with the real-time PCR for detection of C. wilfordii and C. auriculatum DNA. We also tried to realize a quantitative real-time PCR assay using species-specific matK primers, which allowed us to estimate the ratio of C. willfordii and C. auriculatum using varying ratios of mixed genomic DNA template from the two species. The differentiation of intentional and unintentional mixture in this study would be applied to food safety management and can be helpful for protection of consumer's right and cultivators.

Effects of Scolopendrid Water-Alcohol Extract Injection Applied to the Acupuncture Point on the Neuropathic Pain in Rats (백서(白鼠)에서 오공약침(蜈蚣藥鍼)이 신경병증성(神經病症性) 통증(痛症)에 미치는 영향(影響))

  • Lee, Sam-Ro;Koo, Sung-Tae;Kim, Sung-Nam;Hwang, Woo-Jun;Lee, Geon-Mok;Cho, Nam-Geun;Lim, Kyu-Sang;Kim, Sung-Chul
    • Korean Journal of Acupuncture
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    • v.21 no.2
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    • pp.47-67
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    • 2004
  • Objectives : In the present study, the effect of Scolopendrid Water-Alcohol Extract (SWAE) applied to acupuncture point BL23 (Shinsu) on the neuropathic pain was examined. A common source of persistent pain in humans is the neuropathic pain. Anti-convulsant drugs are used to treat the neuropathic pain. In the oriental medicine, Scolopendrid was used for long time to treat convulsant syndrome and back pain, etc. Methods : On the bases of the Scolopendrid clinical application, the effect of SWAE applied to the acupuncture point was tested in the rat model of neuropathic pain. Neuropathic pain was induced by tight ligation of L5 spinal nerve. When rats developed pain behaviors, One hundred microliter of SWAE was applied into the ipsilateral BL23 point at a dose of 10 mg/ml under enflurane anesthesia. The foot withdraw latency of the hind limb was measured for an indicator of pain level after each manipulation. Results : SWAE injection increased the mechanical threshold of the foot in the rat model of neuropathic pain significantly for the duration of 4h, suggesting a partial alleviation of pain. SWAE applied to BL23 point produced a significant improvement of mechanical sensitivity of the foot lasting for at least 4h. However, neither contralateral BL23 point, ST25 (Chonchu) point, nor LR3 (Taechung) point produce as much increase of mechanical sensitivity as ipsilateral BL23 point. And, this increase of mechanical sensitivity was dose-dependent. The improvement of mechanical threshold was interpreted as an analgesic effect. In addition, the analgesic effect of Scolopendrid 4 mg/kg injection is equivalent to that of gabapentin 50 mg/kg injection. The relations between SWAE-induced analgesia and endogenous nitric oxide(NO), inducible NO synthase (iNOS)/neuronal NO synthase (nNOS) were also examined. Results were turned out that both NO production and nNOS/iNOS protein expression which are increased by nerve injury were suppressed by SWAE injection applied to BL23 point. Conclusions : The data suggest 1) that SWAE produces a potent analgesic effect on the neuropathic pain model in the rat and 2) that SWAE-induced analgesia modulate endogenous NO through the suppression of nNOS/iNOS protein expression.

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Myxofibrosarcoma Originating from Chest Wall (흉벽에서 발생한 점액성섬유육종)

  • Cha, Byung-Ki;Lee, Mi-Kyung;Lee, Sam-Youn;Choi, Chong-Bum;Choi, Soon-Ho
    • Journal of Chest Surgery
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    • v.39 no.2 s.259
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    • pp.166-170
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    • 2006
  • Myxofibrosarcoma represents one of the most common soft tissue sarcomas of the extremities in adult and elderly patients. However, it only rarely occurs in the chest wall. A 58 years old woman presented with a huge painless lump (8 ${\times}$ 6.5 cm) in the right lower parasternal area which recurred a few months following excision from General surgery of our hospital. Histology showed an infiltrative deep seated dermal and subcutaneous tumor. It had a distinctive lobular growth pattern with prominent myxoid change and moderate cellularity. Tumor cells were spindleshaped with varying degrees of pleomorphism and frequent mitotic figures. Numerous blood vessels with curvilinear growth pattern were also seen. Immunostains for S-100, CD68, Mac 387, and FX IIIa were all negative, but positive for Vimentin. The histologic feature was of an intermediate grade myxofibrosarcoma. Wide excision was performed and recovered in good condition without any physical disabilities and was discharged at one month later. Currently she is waiting for the skin graft without chemotherapy and radiotherapy.

Serological survey of avian pneumovirus and reovirus in breeders of Jeonbuk province (전북지역 종계에서 avian pneumovirus 및 reovirus 항체가 조사)

  • Lee Jeoung-Won;Shon Ku-Rye;Park Ki-Shung;Kim Youn-Tae;Kim Choo-Cheol;Han Kyu-Sam;Lee Hee-Mum;Song Hee-Jong
    • Korean Journal of Veterinary Service
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    • v.29 no.1
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    • pp.9-18
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    • 2006
  • The ELISA titers to avian pneumovirus (APV) and avian reovirus (ARV) were surveyed to sera of 38 breeder farms (78 blocks, 1,560 hens) in Jeonbuk province during February to October, 2005. In APV, the positive ELISA were detected 36 (94.7%) breeders, 71 (91.0%) blocks, 1,057 (67.8%) hens, and their S/P ratio was 1.940. Regionally, the positivity of 24 breeders farms in the Jeonju, Jeongeup, Namwon, and Jangsu were noted as 100%, whereas 85.7% in Iksan. The positivity to species such as Cobb (20), Ross (13) and Hanhyup-3 (5), all of the breeding farms were detected as positive, 40 (86.9%), 17 (94.4%) and 14 (100.0%) in blocks, 553 (60.1%), 285 (79.2%) and 219 (78.2%) in hens, and their mean S/P ratio were 1.677, 1.769 and 2.254, respectively. The positivity of the breeders vaccinated with ARV, all of the 9 breeder farms (38 blocks) were noted as 100%, but 627 (82.5%) in hens, and its mean S/P ratio was 1.273. Whereas nonvaccinated with ARV were 28 (96.5%) in breeders farms, 38 (95.0%) in blocks, 660 (82.5%) in hens, and the mean S/P ratio was 1.612. In species which were vaccinated with ARV, 11 breeder farms (38 blocks) were noted as 100%, but 82.5% in hens, and their mean S/P ratio were 1.315. Whereas in nonvaccinated with ARV, 25 (92.6%) in farms, 38 (95.0%) in blocks and 660 (82.5%) in hens were positive, and their mean S/P ratio was 1.532.