• Title/Summary/Keyword: Lee Sam

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Effects of wild or mutated inoculants on rye silage and its rumen fermentation indices

  • Paradhipta, Dimas Hand Vidya;Joo, Young Ho;Lee, Hyuk Jun;Lee, Seong Shin;Kwak, Youn Sig;Han, Ouk Kyu;Kim, Dong Hyeon;Kim, Sam Churl
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.6
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    • pp.949-956
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    • 2020
  • Objective: This study was conducted to confirm the effects of new inoculants producing-antifungal or esterase substances on rye silage and its rumen fermentation indices by comparing wild with mutated types. Methods: Rye harvested at dough stage was ensiled into 3 L mini bucket silo (1 kg) for 90 d in triplicate following: distilled water at 20 μL/g (CON); Lactobacillus brevis 100D8 (AT) and its inactivation of antifungal genes (AT-m) at 1.2×105 cfu/g, respectively; and Leuconostoc holzapfelii 5H4 (FD) and its inactivation of esterase genes (FD-est) at 1.0×105 cfu/g, respectively. After silo opened, silage was sub-sampled for the analysis of ensiling quality and its rumen fermentation indices. Results: Among the wild type inoculants (CON vs AT vs FD), FD inoculant had higher (p<0.05) in vitro digestibilities of dry matter and neutral detergent fiber, the total degradable fraction, and total volatile fatty acid in rumen, while AT inoculant had higher (p<0.05) lactate, acetate, and lactic acid bacteria in silage. Silage pH and the potentially degradable fraction in rumen increased (p<0.05) by inactivation of antifungal activity (AT vs AT-m), but lactate, acetate, and lactic acid bacteria of silage decreased (p<0.05). In silage, acetate increased (p<0.05) by inactivation of esterase activity (FD vs FD-est) with decreases (p<0.05) of pH, ammonia-N, lactate, and yeast. Moreover, inactivation of esterase activity clearly decreased (p<0.05) in vitro digestibilities of dry matter and neutral detergent fiber, the total degradable fraction, and total volatile fatty acid in the rumen. Conclusion: This study concluded that FD inoculant confirmed esterase activity on rye silage harvested at dough stage, while AT inoculant could not be confirmed with antifungal activity due to the absence of mold in all silages.

Screening of Flavonoid Compounds with HMG-CoA Reductase Inhibitory Activities (플라보노이드 화합물로부터 HMG-CoA reductase 저해 활성 물질 탐색)

  • Son, Kun Ho;Lee, Ju Yeon;Lee, Jeong Soon;Kang, Sam Sik;Sohn, Ho Yong;Kwon, Chong Suk
    • Journal of Life Science
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    • v.28 no.2
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    • pp.247-256
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    • 2018
  • 3-Hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase inhibitors (statins) are widely used drugs for lowering blood lipid levels and preventing cardiovascular diseases. HMG-CoA reductase is a key enzyme to control the biosynthesis of cholesterol. We have tested HMG-CoA reductase-inhibitory activity on the flavonoids of 98 species in vitro. The anti-hypercholesterolemic activities of flavonoids were studied using an HMG-CoA reductase assay equipped with a 96-well UV plate. This assay was based on the spectrophotometric measurement of the decrease in absorbance, which represents the oxidation of NADPH by the catalytic subunit of HMG-CoA reductase in the presence of the substrate HMG-CoA. Among the clinically available statins, pravastatin was used as a positive control. Among the tested compounds, kuraridin, morin and sophoraflavanone G showed strong inhibition activities. In particular, morin and sophoraflavanone G inhibited HMG-CoA reductase by 45.0% and 54.6% at a concentration of $10{\mu}g/ml$, and the $IC_{50}$ values were calculated to $13.31{\mu}g/ml$ and $7.26{\mu}g/ml$ respectively.

A Case of Primary Endobronchial Leiomyoma (원발성 기관지내 평활근종 1예)

  • Lee, Hee Kyung;Lee, Jae Hyung;Kim, Sang Heon;Kim, Tae Hyung;Sohn, Jang Won;Yoon, Ho Joo;Shin, Dong Ho;Park, Sung Soo;Min, Kyueng Whan;Paik, Seung Sam;Kang, Jung Ho
    • Tuberculosis and Respiratory Diseases
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    • v.61 no.3
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    • pp.273-278
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    • 2006
  • Endobronchial leiomyoma is a rare tumor that accounts for less than 2% of pulmonary benign tumors. A 32 year-old woman was admitted with fever, cough and sputum for a month. She had suffered from intermittent cough over three years. The chest X-ray and chest CT(computed tomography) showed a nodular lesion obstructing the proximal portion of the left lower lobar bronchus and atelectasis of the left lower lobe. Flexible Bronchoscopy detected a mass obstructing the distal portion of the left main bronchus and endobronchial biopsy showed benign smooth muscle cells. There was no abnormal finding in the uterine examination. Therefore this case was diagnosed as primary endobronchial leiomyoma. The lobectomy was performed due to intractable pneumonia and secondary parenchymal destruction. Postoperative course was uneventful and she was discharged in good health.

Low Conversion Loss 94 GHz MHEMT MIMIC Resistive Mixer (낮은 변환손실 특성의 94 GHz MHEMT MIMIC Resistive 믹서)

  • An Dan;Lee Bok-Hyung;Lim Byeong-Ok;Lee Mun-Kyo;Oh Jung-Hun;Baek Yong-Hyun;Kim Sung-Chan;Park Jung-Dong;Shin Dong-Hoon;Park Hyung-Moo;Park Hyun-Chang;Kim Sam-Dong;Rhee Jin-Koo
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.42 no.5 s.335
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    • pp.61-68
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    • 2005
  • In this paper, low conversion loss 94 GHz MIMIC resistive mixer was designed and fabricated. The $0.1{\mu}m$ InGaAs/InAlAs/GaAs Metamorphic HEMT, which is applicable to MIMIC's, was fabricated. The DC characteristics of MHEMT are 665 mA/mm of drain current density, 691 mS/mm of maximum transconductance. The current gain cut-off frequency(fT) is 189 GHz and the maximum oscillation frequency(fmax) is 334 GHz. A 94 GHz resistive mixer was fabricated using $0.1{\mu}m$ MHEMT MIMIC process. From the measurement, the conversion loss of the 94 GHz resistive mixer was 8.2 dB at an LO power of 10 dBm. P1 dB(1 dB compression point) of input and output were 9 dBm and 0 dBm, respectively. LO-RF isolations of resistive mixer was obtained 15.6 dB at 94.03 GHz. We obtained in this study a lower conversion loss compared to some other resistive mixers in W-band frequencies.

Design of the Wideband Notched Compact UWB Antenna (넓은 대역폭이 소거된 소형 UWB 안테나 설계)

  • Kim, Cheol-Bok;Lim, Jung-Sup;Lee, Ho-Sang;Jang, Jae-Sam;Jung, Young-Ho;Jo, Dong-Ki;Lee, Mun-Soo
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.44 no.9
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    • pp.54-62
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    • 2007
  • In this paper, a novel wideband notched compact UWB antenna is designed to satisfy the licensed UWB frequency bandwidth($3.1{\sim}4.8$ GHz, $7.1{\sim}10.2$ GHz) by symmetrically arranging two adjacent sectorial loop antennas. The wideband($4.8{\sim}7.1$ GHz) notch can be obtained by inserting the inverted-L shaped slits on the patch. The designed UWB antenna has return loss lower than -10dB at 3.1 GHz and over, group delay value lower than 1 ns and the linear phase property. The optimized UWB antenna inserted the inverted-L shaped slits has return loss great than -10dB, 5 ns of group delay, nonlinear phase and decreased gain properties over the frequency band, 4.8 GHz to 7.1 GHz.

Studies on Isolation of Pasteurella multocida from Slaughtered Pigs, Microbiological and Serological Properties, Antibiotic Susceptibility of the Isolates and Pathological Observation of Their Lungs (도축돈에서 Pasteurella multocida의 분리, 분리주에 대한 미생물학적, 혈청학적성상 및 항생물질의 감수성조사와 돈폐에 대한 병리학적 관찰에 관하여)

  • Lee Hak-Cheol;Ham Tai-Soo;Chung Yoo-Yeol;Cho Seong-Lyong;Lee Jag-Hyeon
    • Journal of Veterinary Clinics
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    • v.6 no.2
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    • pp.307-318
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    • 1989
  • In recent years much attention has been paid to swine respiratory infection caused by Pasteurella(P) multocida with rapid expansion of pork Industry in Korea. The present study was performed to observe the etiologic situation of P. multocida infection by bacteriological, serological(serotyping) and pathological examinations with the lungs respectively. In addition antibiotic susceptibility test was carried out against the isolated strains of P. multocida. The results obtained are as follows : 1. Eighteen strains(12.8%) wert isolated from the 140 cases of swine lungs examined, and biological and biochemical characteristics of the isolates were the sam as those in the references of other workers, whereas some differences were observed in sugar fermentation and enzyme activity according to the strain of isolates. 2. Capsular serotyping performed on 18 P. multocida revealed that 13 strains(72.2%) were A type and 5 strains(27.8%) were D type, respectively. 3. When serotyping was performed against somatic antigen on 18 strains capsular types of which were identified as described above 9(50%), 3(16.7%) and 4(22.2%) strains belong to 1:A, 3:A and 2:D, respectively, but untypable 2 strains(11.1%) were observed. 4. Antibiotic susceptibility test by employing disc method for 24 kinds of drugs revealed that 15 kinds of antibiotics were sensitive to 18 strains of P. multocida isolated such as ampicillin(l00%), penicillin(100%), cloxacillin(56%), piperacillin(70%), cefotaxime(30%), minocycline(60%), chloramphenicol(95%), erythromycin(39%), kanamycin(17%), gentamicin(70%), amikasin(30%), colistin(78%) and nalidixic acid(5%), respectively, but resistant to 9 kinds of antibiotics such as sulpenicillin, cefazolin, cephalothin, cefametazol, cefoperazone, kitasamycin, oleandomycin. lincomycin and bacitracin. 5. Pathological features of 60 cases of swine lungs indicated that pneumonic .lesions were observed in 38 cases(63.3%) examined by macroscopic finding, in which lesions of 8 cases(13.4%) would correspond to those of mycoplasmal infection, and 30 cases(50%) were similar to viral infection by histopathological finding, whereas 22 cases(36.7%) were considered to be normal by ecropsy or histopathological finding.

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Shape of the model pound net affected by wave and fish behavior to the net - Shape and tension of the model pound net affected by wave - (파동에 의한 모형정치망의 형상변화와 어류대망행동 - 파동에 의한 모형정치망의 형상과 장력변화 -)

  • Lee, Ju-Hee;Kwon, Byeong-Guk;Yun, Il-Bu;Kim, Sam-Kon;Yoo, Je-Bum;Kim, Boo-Young;Kim, Byung-Soo;Lee, Hye-Ok
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.43 no.2
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    • pp.101-115
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    • 2007
  • The pound net fishery is very important one in Korean coastal fishery and it need to grasp the characteristics of the net affected by many factors. It is considered that the structure and the shape of the pound net can be changed by the direction and speed of current, wave height, depth and conditions of sea bed. However, most of all, the speed of current and wave height influence more upon the pound net than any other factors to deform and flutter. In this study, author carried out the experiments with a model of double one-side pound net made by the similarity law as 1:100 scales at a real experimental area, and additionally the model net experiments were conducted in the circulating water channel in Pukyong National University. The author analyzed the data of transformation of shape and tension of the model pound net to recognize the characteristics of the current and wave acting on it. Regardless of the direction of flow affecting on the fish court net or bag net, the deformed angle and depth to the side panel and bottom of box nets becomes bigger as the wave gets higher and the period of wave is faster. The tension in both upward or downward tends to be changed by the speed of wave. Those value of changes occurred similarly in either fish court net or bag net. Generally, when bag net is located at upward of flow, the value of tension was bigger 10% than any other location or nets. Regardless of the setting direction, the tension of the pound net is increased in proportion to flow speed, wave height and period of wave, and it becomes bigger about 15-30% at upward to flow than downward. Where the flow is upward in the court net, the tension in the wave increased to 37% compared to the one in the flow only in the condition of flow of 0.1-0.3m/s. Where the flow is upward in the bag net, the tension in the wave increased to 52% in the flow of 0.1m/s, and the tension increased to 48% in the flow of 0.2-0.3m/s.

An Analysis of Emotional and Cognitive Factors on Acupuncture (침에 대한 정서와 인지요소 분석)

  • Chae, Youn-Byoung;Park, Hi-Joon;Kang, O-Seok;Lee, Jeong-Chan;Park, Kyung-Mo;Lee, Hye-Jung
    • Journal of Acupuncture Research
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    • v.24 no.3
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    • pp.215-229
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    • 2007
  • Objectives : Placebo phenomena have been considered as a confounding factor of clinical trial. Expectancy and belief of acupuncture have not been evaluated quantitatively. The present study was performed to analyze the emotional and cognitive factor .of acupuncture and investigate whether the expectancy of acupuncture treatment is associated with the cognition of acupuncture. Methods : The expectancy and the perception of bodily sensation (PBS) of 22 participants were assessed using self-reported questionnaire. The subjects used the self assessment manikin (SAM) to rate each of the standard affective image of the international affective picture system (lAPS) and other acupuncture-related image. Based on the degree of expectancy, the high expectant (HE) and the low expectant (LE) group were classified. The thermal and pressure pain threshold was objectively evaluated using radiant-heat device and algometer. The degree of expected pain of acupuncture and the actual pain of painful stimulation was subjectively evaluated using facial pain scales (FPS). Results : Using SAlVI analysis, we identified the negative correlation between hedonic valence and arousal dimension on acupuncture-related visual cue. The degree of the PBS and general pain threshold did not show any significant difference between the HE and the LE group. The HE group rated the acupuncture images as more pleasant, more arousing, than the LE group. In addition, we also found that the higher expectancy marked the lower FPS of the expected pain of acupuncture, but not of the actual pain of painful stimulation. Conclusions : Our preliminary study identified the psychological dimensions of acupuncture-related visual cue. These findings indicate that the expectancy of acupuncture could affect the cognition of acupuncture.

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The effect of retinoic acid on radiosensitivity analyzed by linear-quadratic model and apoptosis in head and neck squamous carcinoma cell lines (두경부 편평세포암종세포주에서 retinoic acid가 linear-quadratic 모델을 적용한 방사선감수성과 apoptosis 유발에 미치는 영향)

  • Lee Eun-Sook;Kang Bum-Hyun;Heo Min-Suk;Lee Sam-Sun;Choi Hyun-Bae;Choi Soon-Chul;Park Tae-Won
    • Imaging Science in Dentistry
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    • v.31 no.3
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    • pp.135-143
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    • 2001
  • Purpose : To evaluate the effect of all-trans-retinoic acid on radiosensitivity and radiation-induced apoptosis in NHOK, HEp-2 and FaDu cell lines. Material and Methods: We measured the changes in survival fraction at 2 Gy (SF2), α and β after treatment of retinoic acid (1μM) prior to irradiation with doses of 2, 4, 6 and 10 Gy and correlated the radiosensitizing effect of retinoic acid with them. Also, apoptosis induction was assayed with the flow cytometry on days 1,2, 3, 4 and 5 after irradiation (2, 10 and 20 Gy) combined with retinoic acid. Results and Conclusion: SF2 values for NHOK, HEp-2 and FaDu cell lines were 0.54, 0.64 and 0.41, respectively and the cell line of FaDu was the most radiosensitive. For cell lines of NHOK and HEp-2, pretreatment of cells with retinoic acid resulted in a significant decrease of the SF2 values. The α/β ratios of x-ray survival curve were 8.714 (NHOK), 4.098 (HEp-2) and 11.79 (FaDu). The α/β ratio for NHOK decreased on pretreatment with retinoic acid, whereas those for HEp-2 and FaDu increased. Radiation induced apoptosis in all cell lines but, retinoic acid did not affect the apoptosis.

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The effect of mandibular position and gantry angle on the evaluation of implant site with implant CT (임플랜트 전산화단층사진에서 하악의 위치와 gantry각의 변화가 임플랜트 매식로 평가에 미치는 영향에 관한 연구)

  • Lee Sul-Mi;An Chang-Hyeon;Choi Hang-Moon;Heo Min-Suk;Lee Sam-Sun;Choi Soon-Chul;Park Tae-Won
    • Imaging Science in Dentistry
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    • v.32 no.1
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    • pp.35-39
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    • 2002
  • Purpose: The altered gantry angle during scanning for some multiplanar reconstruction CT program (CT/MPR) may cause distortion of the image. The aim of this study was to ascertain whether there is a image distortion in a reformatted image when the gantry and the object are equally inclined using ToothPix and DentaScan program. Materials and Methods: A resin block model with four cylindrical holes and a human dry mandible were used. Two MPR software packages, ToothPix and DentaScan program, were used for reformatted panoramic images. The block and the gantry were equally inclined at 0°, 15°, and 30°. Results: With ToothPix program, a resin block model with empty holes and a dry mandible showed inclined images in the reformatted panoramic image. Increasing the gantry angle, the depth and inclination of the holes were increased in the reformatted central panoramic images. However, a resin block model with gutta perch a in its holes and a dry mandible with a wire in its mandibular canal didn't show image distortion. With DentaScan program, image distortion was not seen in any situation. Conclusion: ToothPix program may distort the reformatted image when the gantry angle is not at zero degrees. However, with DentaScan program, the patient may be positioned comfortably and the gantry can be adjusted to the patient positioning.

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