• Journal of Veterinary Clinics
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• v.37 no.4
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• pp.198-203
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• 2020

Synovial fluid (SF) contains various factors which may be helpful for corneal stromal healing, such as cytokines, growth factors, hyaluronic acid, and proteins. Therefore, the purpose of this study was to determine the effect of SF on proliferation and migration in canine keratocytes. In order to evaluate the degree of proliferation and migration, canine keratocytes were cultured in DMEM containing 1%, 3%, 5%, or 10% SF. Real-time PCR was performed in a control group and the group treated with 5% SF, in order to measure the expression levels of factors associated with corneal wound healing. These factors included interleukin-1α (IL-1α), hepatocyte growth factor (HGF), transforming growth factor-β (TGF-β), and α-smooth muscle actin (SMA-α). Proliferation assays demonstrated that proliferation was significantly enhanced in groups treated with greater than 3% SF, as compared with that of the control group. In addition, migration in all SF-treated groups was significantly increased as compared with migration in the control group, as measured by migration assays. mRNA expression of IL-1α and HGF was significantly increased and mRNA expression of TGF-β and SMA-α was significantly decreased in the cells treated with 5% SF. These findings suggest that SF may promote corneal wound healing.

• Korean Journal of Pharmacognosy
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• v.34 no.3 s.134
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• pp.256-262
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• 2003

Lonicerae Flos (LF) has been used as an anticancer, anti-viral, and anti-inflammatory agent in traditional herbal medicine. In this study, induction of rat growth hormone (rGH) by addition of methanol (MeOH) extract of LF of Lonicerae (L.) Folium or several constituents of L. Folium were carried out in the pituitary cell culture system. Induced rGH level by addition of 70% MeOH extract of LF was increased to $732.65{\pm}105.64%$ of control (n=18, p<0.01), however, the other sequential fractions were not significantly different from the control. Ochnaflavone, a constituent of L. Folium, induced rGH level in the cell culture to $329.73{\pm}160.00%$ of control (n=6, p<0.01). An I..v. injection of the MeOH extract of LF did not increase plasma rGH level in anesthetized rats. Unfortunately, the MeOH extract of LF induced prolactin and LH release about 7 and 5 fold of the control, respectively (p<0.05, each). In conclusions, 70% MeOH extract of LF exerted induction of rGH release in rat pituitary cell culture. Further studies to investigate mechanisms of the inducded rGH by LF are in progress.

• Journal of Korean Society of Water and Wastewater
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• v.13 no.3
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• pp.73-82
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• 1999

In this study, the optimal analysis for pipe network is performed for the combined ideal pipe network system(CASE 1, CASE 2 and CASE 3) which is composed of 25 nodes, 41 elements, and 1 fixed nodal head with evaluating pressure variation distribution of main and branch in grid composed drainage pipe network. The linear analysis technique used as the analysis method in this study, the KYPIPE being used extensively as the linear technique to design and analysis of pipe network is applied. Firstly, in the analysis of pipe network, the CASE 2 and CASE 3 supply same thing(value) in the result of considering the total flow provided each pipeline, but in the general intension in the case of CASE 2, relative width of supply is more large than CASE 1 and CASE 3. Secondly, in the analysis technique of pipe network, CASE 3 is analysed largest as a result of comparing with same heads, and in the order of their size CASE 2 and CASE 1 were determined but the difference doesn't appear to be obvious. Thirdly, as the result of determining main factor, pressure in the design and analysis of net work. CASE 3 is from Node 3 to 25 than CASE 1 and CASE 2 and it is determined in the order of their size, CASE 2 and CASE 1. Finally, in this study, discharge flow distribution is evaluated in the same condition with 3-type CASE in the case of branch position for designing optimal composed drainage pipe network. As the result of that, branch pipe perform. Therefore, it is thought that the efficient and reasonable management of water supply and sewerage design will be possible if it give all our energies to study at the pipe system design in and out of country in the future.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.26 no.6_spc
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• pp.644-650
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• 2016

Track maintenance works based on track geometry recordings are essential to enhance the safety and comfort of railway transportation. Usually, the track irregularity has been measured by a special inspection trains which all were imported from abroad. Because the inspection train speed is limited under 160 km/h, it takes a long time to inspect railways and there is difficulty in daytime operation. To solve this problem, we started to develop a track geometry measuring system (TGMS) with measurement speed up to 300 km/h which can be installed in commercial vehicles such as HEMU-430X. In this paper, we introduce a newly developed inertial TGMS and propose two inertial navigation system (INS) algorithms (method A, B) for measuring track geometry. In order to investigate advantage and disadvantage of each algorithm, we performed vibration test of the TGMS, which was simulated by 6-axis shaking table. Through the vibration test, we analyzed the influence of vehicle vibration on the TGMS which will be installed on bogie frame. To the vibration test, two methods satisfied the required accuracy of track geometry measurement under the level of the actual vehicle vibration of HEMU-430X train. Theoretically, method A is sensitive to vehicle vibration than method B. However, HEMU-430X's bogie vibration frequency range is out of interest range of measurement system. Therefore, method A can also apply the HEMU-430X train.

• Journal of Physiology & Pathology in Korean Medicine
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• v.21 no.3
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• pp.617-625
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• 2007

Samul-Tang (SMT) has been used for nourishing of the blood, hematopoiesis as a herbal medicine history. The purpose of this study is to find out anti-allergic inflammatory reaction of SMT. To clarify the mechanism, the effect of SMT on vascular permeability of rat cutaneous tissue and histamine and cytokines (IL-6, IL-8, TNF-${\alpha}$) release from mast cells were observed. The results are the pretreatment with SMT significantly decreased the compound 48/80-induced degranulation and histamine release from RPMC, SMT also inhibited the anti-DNP lgE-induced increment of vascular permeability of rat cutaneous tissue. SMT significantly reduced the PMA plus A23187-induced increment of expression of IL-6, IL-8, and TNF-${\alpha}$ in HMC-1 Cell. The Present study provide evidence that SMT inhibits mast cell-derived inflammatory allergic reactions by blocking histamine release and pro-inflammatory cytokine expression, and suggest the mechanisms of action. Furthermore, in vivo and in vitro anti-allergic effect of SMT suggests a possible therapeutic application of this agent in inflammatory allergic diseases.

• Korean Journal of Acupuncture
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• v.26 no.4
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• pp.53-66
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• 2009

Objective : Acupuncture has been widely used throughout the world for the treatment of knee osteoarthritis (knee OA). This study investigated whether acupuncture, particularly Sa-am acupuncture, could be effective in relieving pain and improving the symptoms of knee OA. Method : This study was conducted as a prospective, randomized, controlled, and patient- and investigator-blinded clinical trial. Forty volunteers with knee OA participated in the study. All participants were screened through an inclusion and exclusion criteria. Thirty four participants completed the clinical trial. In total, forty subjects were randomly selected to receive Sa-am acupuncture. Eight sessions of acupuncture were given at the contralateral side of the problematic knee for 4 weeks. Korean translation of Western Ontario and McMaster Universities Osteoarthritis Index (KWOMAC) scores were measured twice: at the beginning and end of the clinical trial period. Both the Patient Global Assessment and physical health scores based on the 36-Item Short-Form Health Survey were also used to measure the results. Results : Compared to the pre-trial scores, the Sa-am acupuncture group (n=34) showed a significant decrease in KWOMAC total scores according to a paired t-test. The Sa-am acupuncture group also showed significant improvement in the Patient Global Assessment when compared to the pre-trial. Conclusions : Sa-am acupuncture for knee OA resulted in an improved KWOMAC total score. This was mostly driven by the function component score that was greatly affected by acupuncture. However, further studies with expanded designs are needed to solidify this finding with scientific rigor.

• Korean Journal of Organic Agriculture
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• v.16 no.3
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• pp.299-308
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• 2008

In this study, the effect of commercial organic growth medium amended with different ratios of vermicast on the growth of rice seedlings. The amended ratios of vermicast were 0% (control), 5%, 10%, 20% and 40%, respectively. A bioassay with two rice varieties (Chucheng and Black) was conducted to assesses the amendment effect of vermicast on the seedling growth in two potting methods. In potting treatment, the Chucheng variety with late maturity showed significantly higher values on growth parameters excepted root weight (RW) up to 20% and Black variety with early maturity up to 10% amended with vermicast, respectively. In floor layering treatment, all of the growth parameters significantly increased up to 5% amended with vermicast in both rice variety. The seedling growth of rice in floor layering treatment attained the highest values at lower amended ratios of vermicast than those of potting treated only, it may correspond with increased uptake of nutrient by elongated root grown under the layering amendment of vermicast. Vermicasts can be used as soil amendments or components of plant growth medium for seeding organic agriculture.

• Korean Journal of Organic Agriculture
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• v.22 no.3
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• pp.413-421
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• 2014

This study was conducted into the effects of cover crops among the hairy vetch, red clover, rye, and hairy vetch+rye on the soil chemical and physical properties, and fruit yield in mature 'Fuyu' persimmon (Diospyros${\times}$kaki Thunb.) trees. The shallow-rooted red clover had poor dry matter production, resulting in the lowest coverage (66%) on the orchard floor. In contrast, the highest dry matter production observed in rye and hairy vetch+rye. Estimated N, P and K production from the cover crops were the highest on the hairy vetch+rye plots, increasing soil chemicals at a depth of 0-30 cm soil. Rye or hairy vetch+rye treatments decreased the soil bulk density and solid phase. As the hairy vetch+rye treatment increased fruit yield and sugar contents, it could be proposed as an suitable cover crop for improving productivity of persimmon trees.

• Korean Journal of Food Science and Technology
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• v.50 no.1
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• pp.37-43
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• 2018

Optimization of the lactic acid fermentation process was carried out to produce an old antler extract fortified with ${\gamma}$-aminobutyric acid (GABA). An old antler extract (OAE; 5%, w/v) obtained using a herbal extractor showed the highest contents of solids (1.75%) and proteins ($980{\mu}g/mL$). It also showed the highest total amino acid contents of $13,659{\mu}g/mL$, with glycine, proline, and glutamine concentrations of 1,945, 3,405, and $1,641{\mu}g/mL$, respectively. For the over-production of GABA, OAE was fermented with Lactobacillus plantarum EJ2014 in the presence of 0.5%, 1.5% glucose, and 3.5% MSG at $30^{\circ}C$ for 7 days. The fermented OAE showed high viable cell count of $2.0{\times}10^8CFU/mL$, pH of 6.56 and 0.77% acidity after 7 days. In particular, the acidity was greatly decreased by fermentation for 3 days, and 1.4% GABA was produced by the efficient conversion of the substrate, mono sodium glutamate.

• The Journal of the Korean Society for Microbiology
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• v.8 no.1
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• pp.27-32
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• 1973

Listeria monocytogenes human infection is a relatively rare disease which usually is meningitis in newborn babies. The organism was isolated from blood cultures of a 52 year old female patient with meningitis. It was considered that the underlying disease, i.e. S.L.E., and the steroid therapy which the patient had been receiving played some role for the Listeria infection. The isolate was showing characteristics of L. monocytogenes, i.e. diphtheroid like morphology, motility with four peritrichous flagella, hemolytic small colonies on blood agar, growth in the presence of 7.5% salt and at 4 C, and inducing monocytosis in an experimentally infected rabbit. Serologically the organism was identified as L. monocytogenes 4b. The isolate was showing susceptibility to many antibiotics tested including ampicillin, penicillin and tetracycline which were the recommended drugs of choice for the treatment of Listeriosis. It is the general opinion that Listeriosis is not so rare as literatures are showing. It is considered that some of the isolate of the organism from clinical specimens are mistakenly discarded due to the fact that the organism shows diphtheroid like morphology and that not many laboratories are able to recognize the organism. Literatures are seen which emphasize more careful examination of gram positive bacilli with diphtheroid like morphology especially when they are isolated from blood or from spinal fluid of patient.