• Journal of Korean Neurosurgical Society
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• v.29 no.1
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• pp.15-22
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• 2000

Objective : This study was undertaken to evaluate the benefits and risks of the stereotactic biopsy in brain lesions. We assessed the diagnostic accuracy and morbidity rate associated with the stereotactic biopsy. Methods : The authors present a review of 47 patients, who underwent stereotactic biopsy using Cosman-Roberts-Wells(CRW) stereotactic apparatus during last six years. Results : Target locations were supratentorial in 36 cases, infratentorial in 9 and multiple in 2. According to pathological diagnosis, the largest group was neoplasm(29) followed by infection(9), infarction(2), cyst(2), and non-specific(5). Definitive diagnosis could be made in 42 of 47 cases(89.4%). When the mass lesion had been suspected as neoplastic condition, the diagnostic rate was 96.7%(29/30). It was being much higher than that of non-neoplastic lesion, 76.5%(13/17). The treatment modality was changed in 15 cases(32%) because the result of stereotactic biopsy was different from clinical diagnosis. Subsequent craniotomy after stereotactic biopsy was then performed in 6 cases, and the pathological diagnoses were precisely coincident in all of these cases. There were two complications(4.3%) : One intratumoral hemorrhage in glioblastoma and a transient hemiparesis in benign astrocytoma. There was no mortality in this series. Conclusion : The precise histological verification is crucial to determine the adequate treatment modality in intracranial lesions. Stereotactic biopsy is a safe and accurate diagnostic procedure for intracranial lesions with a low complication rate.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.26 no.2
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• pp.198-203
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• 1993

The cyst of Scrippsiella trochoidea from the surface mud in Masan Bay was germinated in the incubator for the culture. This species was one of dinoflagellates responsible for the early spring bloom in the southern coastal water of Korea. The culture experiments were carried out under the various gradients of environmental factors to know their effects on the population growth of this dinoflagellate. With respect to the effects of environmental factors on the growth, it was proved that the maximum cell growth was occurred at 4,000 lux of light intensity, salinity $30\%0$ and temperature $20^{\circ}C$. When 0.25ml/ml of the filtrates of Skeletonema costatum culture medium and the supernatants of soil extracts were added to growth medium as organic growth stimulants, both materials enhanced the population growth. In the fatty acid composition of S. trochoidea, $C_{16:0}$ was the major component, and $C_{18:0},\;C_{18:1},\;C_{22:0}\;and\;C_{22:1}$ were a minor components.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.48 no.5
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• pp.681-687
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• 2015

We investigated the effects of feeding rate on the growth performance of growing and sub-adult olive flounder Paralichthys olivaceus, and the optimum feeding rate at high water temperature (25-31℃). In experiment I, two replicated groups of fish (113 g) were fed a commercial diet (CD) at rates of 0, 0.7, 1.2, 1.7, 2.2, and 2.4% (satiation) body weight (BW) per day for 4 weeks. In experiment II, two replicated groups of fish (313 g) were fed a CD feeding rates of 0, 0.3, 0.5, 0.7, 0.9, and 1.0% (satiation) BW per day for 4 weeks. In experiment I, the weight gain (WG) and specific growth rate (SGR) of fish fed at 0.7 and 0.9% BW per day was significantly higher than that of unfed fish (0%) and fish fed at 0.3, 0.5, and 1.0% (satiation) BW per day. In experiment II, the WG and SGR increased significantly as feeding rate increased from 0 to 2.2% BW per day, but decreased in the satiation group (2.4%). Broken-line regression analysis of WG showed that the optimum feeding rate of growing and sub-adult olive flounder were 1.98% and 0.55% BW per day, respectively, at the high water temperature.

• Journal of Microbiology and Biotechnology
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• v.23 no.10
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• pp.1381-1385
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• 2013

The centipede Scolopendra subpinipes mutilans is a medicinally important arthropod species. However, its transcriptome is not currently available and transcriptome analysis would be useful in providing insight into a molecular level approach. Hence, we performed de novo RNA sequencing of S. subpinipes mutilans using next-generation sequencing. We generated a novel peptide (scolopendrasin II) based on a SVM algorithm, and biochemically evaluated the in vitro antimicrobial activity of scolopendrasin II against various microbes. Scolopendrasin II showed antibacterial activities against gram-positive and -negative bacterial strains, including the yeast Candida albicans and antibiotic-resistant gram-negative bacteria, as determined by a radial diffusion assay and colony count assay without hemolytic activity. In addition, we confirmed that scolopendrasin II bound to the surface of bacteria through a specific interaction with lipoteichoic acid and a lipopolysaccharide, which was one of the bacterial cell-wall components. In conclusion, our results suggest that scolopendrasin II may be useful for developing peptide antibiotics.

• 한국태양에너지학회:학술대회논문집
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• 2009.04a
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• pp.190-195
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• 2009

Since the industrial revolution, the global environmental problems such as greenhouse gas accumulation and the average temperature increase have caused people's attention. 'Low Carbon, Green Growth' was presented to cope with these global concerns, as one of main policies of 2008 in Korea. The paradigm of a green urban development is started to concern the whole city's energy problems owing to realize 'Low Carbon, Green Growth' in the urban side. The government established a nation's basic energy plan for 20 years, and some local cities made efforts to develop new renewable energy such as the solar, wind and water energy which are suitable to each city's character. As a part of these efforts, the concept of U-Eco city is newly appeared to reflect upon ubiquitous technique, urban ecology and the next generation energy system. However, urban plan is difficult to adopt this next generation energy system with existing laws, regulations and technical systems. The new executive and systematic system is needed to realize the U-Eco city U-Eco for the management of an efficient city. In this study, the authors investigate the concept of the next generation energy system and U-Eco city to realize the energy-efficient city plan and analyze problems to occur during the application of them in an existing city plan. Then, the authors show the remedies to deal with occurred problems.

• Journal of Korean Society on Water Environment
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• v.21 no.5
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• pp.511-515
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• 2005

CBOD(carbonaceous BOD) decay rate was investigated for the execution of water quality model in Nakdong-Rive basin. Estimation of laboratory-derived CBOD decay rate, $k_l$ and CBOD decay rate in natural waters, $k_d$ were carried out. Hydraulic factors were applied for the calculation of $k_d$. Values of biochemical oxygen demand were investigated in Weagwan, Koreong, Jeokpo, Namgi and Mulgeom sites for the four times. The ranges of $k_l$ value were $0.04{\pm}0.01{\sim}0.14{\pm}0.03$. The values of $k_l$ in upstream sites were much larger than those in the downstream sites. The values of $k_d$ were 0.025, 0.036, 0.005 and 0.001 at Weagwan, Jeokpo, Namgi and Mulgeom, respectively, indicating that values of $k_d$ were generally larger than those of $k_l$.

• Korean Journal of Veterinary Research
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• v.43 no.1
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• pp.103-112
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• 2003

A multiplex PCR assay, which allows simultaneous detection of vancomycin resistant genotypes and Enterococcus species-specific genes, was developed. Vancomycin resistant enterococci (VRE) from chickens and humans could be detected for vanA, vanB, vanC-1, vanC-2, $ddl_{E.faecium}$ and $ddl_{E.faecalis}$ by multiplex PCR. Eight isolates of VRE from humans (n=11) had $ddl_{E.faecium}$ and vanA, and 3 isolates of the VRE had $ddl_{E.faecium}$ and vanB. One isolate of VRE from chickens (n=6) had $ddl_{E.faecium}$ and vanA, and 5 isolates of the VRE had only vanA. E. faecium, E. faecalis, E. gallinarum and E. casseliflavus were also confirmed for the species-specific gene by multiplex PCR. This multiplex PCR could detect E. faecium, E. faecalis, E. gallinarum, E. casseliflavus, vanA, vanB, vanC-1 and vanC-2, simultaneously. The PCR assay established in the present study can be an alternative to time-consuming biochemical tests and antibiotic susceptibility tests of Enterococcus spp.

• International Journal of Industrial Entomology and Biomaterials
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• v.10 no.1
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• pp.11-17
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• 2005

In our research to identify gene involved in the cuticle protein, we cloned a novel cuticle protein gene, ApCP15.5, from the Chinese oak silkmoth, Antheraea pernyi, larvae cDNA library. The gene encodes a 149 amino acid polypeptide with a predicted molecular mass of 15.5 kDa and a pI of 9.54. The ApCP15.5 contained a type-specific consensus sequence identifiable in other insect cuticle proteins and the deduced amino acid sequence of the ApCP15.5 cDNA is most homologous to Tenebrio molitor-C1B ($43\%$ protein sequence identity), followed by Locusta migratoria-76 ($42\%$ protein sequence identity). Northern blot and Western blot analyses revealed that the ApCP15.5 showed the epidermis-specific expression. The expression profile of ApCP15.5 indicated that the ApCP15.5 mRNA expression was detected in the early stages after larval ecdysis and larval-pupal metamorphosis, and its expression level was most significant on the first day of larval ecdysis and pupal stage. The ApCP15.5 was expressed as a 15.5 kDa polypeptide in baculovirus-infected insect cells.

• Journal of Korean Academy of Nursing Administration
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• v.9 no.1
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• pp.51-61
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• 2003

Purpose : This study was performed to measure the level of stress that is perceived by nurses and nursing students and promote the effect of clinical nursing education. Method : the questionnaire measuring the level of stress was developed by researchers and distributed to 200 nursing students and 300 nurses in three provincial city, Korea The data was collected by self-reporting from March 1 to 15 of 2002. For data analysis, SPSS/PC was used. Result : The stress score of nursing students was higher than that of nurses. But it was not significant different in statistics. The items that had a significant difference between two groups were 17 items among the 35 items. The factors that had a significant difference between two groups were 3 factors among the 4 factors. In relationship between the subjects' demographic characteristics and their stress, the only nursing unit had a relationship to their stress. Conclusion : The level of stress being perceived by nursing students and nurses was no significant different in statistics and the subjects' demographic characteristics that had a relationship to their stress was only the nursing unit.

• International Journal of Advanced Culture Technology
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• v.8 no.3
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• pp.231-240
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• 2020

In this paper, we present to evaluate physiological activity of Asparagopsis armata extraction. After extraction with Asparagopsis armata using hydrothermal and supercritical carbon dioxide, various physiological activities were examined. The total concentration of polyphenol compounds was determined to be 18.85 mg/g of hydrothermal Asparagopsis armata extraction and 14.74 mg/g of supercritical Asparagopsis armata extraction. In DPPH radical scavenging assay, ascorbic acid was used as positive antioxidant control. In ABTS radical scavenging assay, ascorbic acid was used as positive antioxidant control. The percentage of inhibition and IC50 were measured. The IC50 of Asparagopsis armata extraction is 261.44ppm and the IC50 of supercritical Asparagopsis armata extraction is 153.98 ppm. The elastase inhibitory assay showed concentration dependence and the IC50 of hydrothermal Asparagopsis armata extraction is 3387 ppm and the IC50 of supercritical Asparagopsis armata extraction is higher than 2500 ppm. In mushroom tyrosinase inhibition experiments, tyrosinase inhibition's IC50 of supercritical Asparagopsis armata extraction was 248.06. In the SOD-like experiments, the concentration-dependent results were showed and IC50 of hydrothermal Asparagopsis armata extraction is 845.29 ppm. In the antimicrobial experiments, maximum clear zones of supercritical Asparagopsis armata extraction represented 23.00 mm in Propionibacterium acnes. In the other hand, in experiments with the same conditions, hydrothermal Asparagopsis armata extraction had no effect in all strains.