• Title/Summary/Keyword: Lee Sam

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Effects of Food Waste Compost and Mineral Nitrogen Application Level on Nutritive Value and Nutrient Yields of Orchardgrass (Dactylis giomerata L.) (음식물쓰레기 퇴비와 무기태 질소 시용이 오차드그라스의 사료가치와 양분수량에 미치는 영향)

  • Jo, Ik-Hwan;Lee, Ju-Sam;Jun, Ha-Joon;Lee, Ju-Hee;Kim, Min
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.20 no.3
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    • pp.169-176
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    • 2000
  • This experiment was carried out to investigate the effects of application levels of food waste compost andmineral nitrogen in 3 cuttings per annurn on the nutritive value and nutrient yields of orchardgrass (Dactylisglomerata L.). Annual food waste compost (FWC) and mineral nitrogen were applied at levels of 0, 10, 20,40 and 60 ton ha-', and 0, 90, 180 and 270 kg ha-', respectively. The contents of crude protein (CP, %) oforchardgrass were significantly higher at the application levels of 40 ton ha-' yr-' by FWC than those atapplication levels of 0 ton ha-' yr-' (p<0.05), the contents of neutral detergent fiber (NDF, %), acid detergentfiber (ADF, %) and hemicellulose were also higher in FWC applied plots, except for the FWC levels of 10and 60 ton ha-' yr-'. The contents of total digestible nutrient (TDN, %) and relative feed value (RFV) oforchardgrass were significantly higher at FWC levels of 10 and 60 ton ha-' yr-' than at levels of 0 ton ha"yr-'. Annual yields of CP and TDN were increased with increase the FWC levels. The highest contents ofCP of orchardgrass were obtained at 1st cut, NDF and ADF at 2nd cut. As the mineral nitrogen fertilizationwas increased, the contents of CP, NDF, ADF and hemicellulose of orchardgrass were significantly increased,but TDN and RFV were decreased. .Annual yields of CP and TDN of orchardgrass were significantlyincreased with increase the mineral nitrogen fertilization.(Key words : Food waste compost, Mineral nitrogen, Crude protein, Neutral detergent fiber, Acid detergentfiber)rgent fiber, Acid detergent fiber)

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Oviposition and Colony Development of the Bumblebees, Bombus ignitus and B. terrestris depending on Different Pollen

  • Yoon, Hyung-Joo;Kim, Sam-Eun;Lee, Kyeong-Yong;Lee, Sang-Beom;Park, In-Gyun
    • International Journal of Industrial Entomology and Biomaterials
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    • v.11 no.2
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    • pp.99-105
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    • 2005
  • We investigated oviposition and colony development of Bombus ignitus and B. terrestris depending on different pollen. In five kinds of pollen blend, the colony development in fresh-freezing pollen blend produced in Korea (Korea-FFP) was the best performance, although egg-laying characteristics is lower than that of pollen blend for oviposition imported from Korppert company (Kopport-FOP). The Kopport-FOP proved that it was suitable to use for oviposition of bumblebees. The Korea-FFDP, freezing dried-fresh pollen blend produced in Korea, is lower rather than the Korea-FDP although it is similar to the KopportFOP in colony development. It is not efficient to use commercial pollen for bumblebee because it is expensive in cost. The dried pollen blend for honeybee feeding imported from China (China-DP) was not suitable for rearing of bumblebee because it did not form colony although the worker emerged. In types of pollen, the oviposition and colony development of B. ignitus were not affected by the fresh-freezing pollen and dried-freezing pollen. This result also indicated that dried pollen, dried in the shade for 5 - 6 days, is possible to use as commercial pollen for bumblebee reproduction.

Antioxidative Activity and Irritation Test of a Complex Herbal Bath Extracted from Korean Traditional Plants

  • Chang Zhiqiang;Kim Young-Hoan;Song Jae-Chan;Kim Nam-Woo;Lee In-Seon;Lee Sam-Pin;Kim Jong-Choon;Park Seung-Chun
    • Toxicological Research
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    • v.21 no.1
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    • pp.45-49
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    • 2005
  • The purpose of this study is to evaluate the free radical scavenging ability and xanthine oxidase inhibitory activity of a complex herbal bath consisted of Artemisiae argyi folium, Angelicae sinensis radix, Ligustici wallichii radix and Angelicae tuhuo radix, and its potential irritation response were also tested for safety use in the rabbits. For antioxidative activity, the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of the complex herbal bath were examined at five different concentrations (0, 250, 500, 1000 and 2000 ㎍/ml). The concentration of the complex herbal bath required for scavenging DPPH free radical by 50% was 897.2 ㎍/ml. In the inhibition of xanthine oxidase (XO) activity, the concentration of the complex herbal bath required for 50% of inhibition was 221.4 ㎍/ml. In the skin irritation study in rabbit, all animals survived for the duration of the study and the examined skin exhibited no edema, erythema, and eschar formation. In the ocular irritation study in rabbit, after application of the sample to eyes, all of the eyes were normal. In summary, the complex herbal bath has potent antioxidant effects against the DPPH radical and XO and was considered to be a non-irritation bath for safety use.

Antigastritic and Anti Helicobacter pylori of Trifolirhizin from Sophora Radix (고삼 Trifolirhizin의 항위염 및 항 H. pylori 균 효과)

  • Kang, Min-Hee;Lee, Jung-Hun;Lee, Je-Hyuk;Cho, So-Yean;Choi, Jae-Sue;Kim, Yeong-Shik;Kang, Sam-Sik;Jeong, Choon-Sik
    • Korean Journal of Pharmacognosy
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    • v.37 no.4 s.147
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    • pp.266-271
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    • 2006
  • Sophorae Radix, the dried roots of Sophora flavescens Aiton (Leguminosae), has been used in oriental traditional medicine for treatment of skin and mucosal ulcers, sores, gastrointestinal hemorrhage, diarrhea, inflammation and arrhythmia. Present study was carried out for the gastroprotective effect of trifolirhizin from Sophora flavescens. This reports were evaluated antibacterial activity against Helicobacter pylori and HCl ethanol-induced gastric lesion in rats and showed the significant effectiveness. In pylorus ligated rats, the treatments of trifolirhizin showed decrease in the volume of gastric secretion and acid output. Also we evaluated the antibacterial activity against H. pylori treated with methanol extract and trifolirhizin from Sophora flavescens had a equivalent antibacterial activity with ampicillin against H. pylori at the dose of $100{\mu}g/mL$. It may be regarded that the antigastritic effects and antibacterial activity of trifolirhizin from Sophora flavescens are originated from reduction of total acid output identified by gastric secretion reduce, free radical scavenging effects and the antibacterial activity against H. pylori.

Effects of Water and Methanol Extracts of Cricket (Gryllus bimaculatus) on Alcohol Metabolism (귀뚜라미의 물 및 메탄올 추출물이 알코올 대사에 미치는 효과)

  • Lee, Yong-Woo;Lim, Soon-Sung;Ryu, Kang-Sun;Lee, Heui-Sam;Kim, Ik-Soo;Kim, Jin-Won;Ahn, Mi-Young
    • Korean Journal of Pharmacognosy
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    • v.35 no.2 s.137
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    • pp.175-178
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    • 2004
  • The cricket has been used in East Asia as crude drugs for treating fever and hypertension, and is presently reared as a pharmaceutical insect in China and a food for animals. For the purpose of evaluating protective extracts against alcohol-induced toxicity, the extracts of the cricket (Gryllus bimaculatus) were examined in animal models acutely administered alcohol by the cricket in ICR-mice. Water and methanol extracts from the cricket, were found to cause a significant decrease (37%) in the blood ethanol concentration as well as enhancement of liver mitochondrial alcohol dehydogenase (ADH) and acetaldehyde dehydogenase (ALDH) activitieson on a single intraperitoneal administration in mice. Futhermore methanol extract was demonstrated to exhibit more potent enhancing activity on ethanol metabolism than water extract. These results suggest that water/alcohol extract of G. bimaculatus may be used as a food for reducing the toxicity of alcohol.

Phytochemical Studies on Paeoniae Radix (4);Cerebrosides and Other Constituents

  • Kim, Yoon-Jung;Yean, Min-Hye;Lee, Eun-Ju;Kim, Ju-Sun;Lee, Je-Hyun;Kang, Sam-Sik
    • Natural Product Sciences
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    • v.14 no.3
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    • pp.161-166
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    • 2008
  • A mixture of sixteen cerebrosides, which comprised four cerebroside molecular species (PL-1 ${\sim}$ PL-4) was separated from the roots of Paeonia lactiflora. The structures of cerebrosides were characterized as $1-O-{\beta}$-D-glucopyranosides of phytosphingosines, which comprised a common long-chain base, (2S,3S,4R,8E/Z)-2-amino-8-octadecene-1,3,4-triol with eight fatty acids or 2-hydroxy fatty acids of varying chain lengths ($C_{16}$, $C_{18}$, $C_{20-26}$) linked to the amino group. Aralia cerebroside and its 8Z isomer (PL-1), $1-O-{\beta}$-D-glucopyranosyl-(2S,3S, 4R,8E/Z)-2-[(2'R)-2'-hydroxytetracosanoylamino]-8-octadecene-1,3,4-triol (PL-2), $1-O-{\beta}$-D-glucopyranosyl-(2S,3S,4R, 8E/Z)-2-[(2'R)-2'-hydroxydocosanoylamino]-8-octadecene-1,3,4-triol (PL-3), and $1-O-{\beta}$-D-glucopyranosyl-(2S,3S,4R, 8E/Z)-2-[(2'R)-2'-hydroxytricosanoylamino]-8-octadecene-1,3,4-triol (PL-4) were identified as major components of these cerebroside molecular species. All the major cerebrosides were shown to be a mixture of geometrical isomers (8E and 8Z) of phytosphingosine-type glucocerebrosides possessing 2R-hydroxy fatty acids. In addition, three ${\beta}-sitosterol$ derivatives and adenosine were also separated. The structures of these isolates have been determined on the basis of chemical and spectroscopic evidence.

Improved Nipple Location in Young Korean Patients

  • Lee, Bo Hyung;Kwon, Yu Jin;Park, Jang Wan;Hwang, Jae Ha;Kim, Kwang Seog;Lee, Sam Yong
    • Archives of Plastic Surgery
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    • v.41 no.6
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    • pp.748-752
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    • 2014
  • Background Gynecomastia is benign enlargement of breast tissue in males and is fairly common. Mastectomy not only helps in improving the shape of anterior chest, but can also improve the location of nipple. Therefore, a principle element of mastectomy design is defining the normal location of nipple based on major anatomical reference points. Here, the nipple location was compared for before and after gynecomastia surgery. In addition, the same was also compared between male patients undergoing gynecomastia surgery and control group of subjects without gynecomastia. Methods We retrospectively analyzed gynecomastia patients who underwent conventional subcutaneous mastectomy. Preoperative and postoperative anatomical landmark distances and chest circumferences were measured and compared to the same anthropometric data from 20 healthy adult male controls. Results Nipple locations were compared among 13 patients and 20 controls. The mean weight of resected breast tissue was 246 g, and overall patient satisfaction grade was 4.3 out of 5. In the patient group, the slopes for the height-distance from the sternal notch to the nipple and chest circumference-distance between the mid-line of the sternum and the nipple were 0.175 and 0.125 postoperatively, respectively. The slopes of the control group were 0.122 and 0.177, respectively; these differences were statistically significant (P<0.05). Conclusions Nipple positions were considerably lower in patients with gynecomastia than in control subjects. Subcutaneous mastectomy was associated with mild elevations, but postoperative locations were still lower compared to controls. Further efforts are needed to improve the location of postoperative nipple-areola complex in patients with gynecomastia.

The Kinematic Analysis of Gliding Type and Delivery Phase in Each Trails during Shot-Putting - Focusing on Lee, Hyung-Keun, Player in Men's High School Youth Group - (고등부 남자 포환던지기 선수의 시기 별 글라이드 유형과 딜리버리 국면의 운동학적 분석 - 고등부 이형근 선수를 중심으로 -)

  • Kim, Tae-Sam;Ryu, Ji-Seon
    • Korean Journal of Applied Biomechanics
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    • v.22 no.2
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    • pp.159-171
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    • 2012
  • The purpose of this study was to provide information about kinematic variables of the gliding and delivery motion of Hyung-Keun Lee, a high school shot putter who was ranked 1st at the 2011 National Sports Festivals. Three-Dimensional motion analysis using a system of 4 video cameras at a sampling frequency of 60 Hz was conducted during shot-putting events at the 2011 National Sports Festivals. During the gliding and delivery phase of the player the results showed following characteristics; 1) The gliding technique types of the player appeared to be the short-long technique as the gliding and stance length ratio were $42.3{\pm}3.87$ % and $57.7{\pm}3.87$ %, respectively. In addition, the trajectory of shots during the gliding and delivery phase showed different trajectory patterns with "S-shaped" type of elite players due to the deviation from a central axis of the APSS (athletic-plus shot system). 2) The horizontal velocity of COG made from gliding should maintain the velocity during transition and release phase, but the player showed a small momentum for a gradual decrease of velocity. 3) Therefore, the player requires to adjust an appropriate ratio between gliding and stance length with a strong muscle power at the trunk, throwing arm, and the lower extremity during gliding and delivery phase.

Analysis of Diapause-Associated Changes in Silkworm Egg Proteins

  • Go, Hyun-Jeong;Hwang, Jae-Sam;Kim, Young-Tae;Kim, Hyun-Su;Kang, Seok-Woo;Chang, Jong-Su;Lee, Sang-Mong;Lee, Bong-Hee;Seong, Su-Il
    • International Journal of Industrial Entomology and Biomaterials
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    • v.9 no.1
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    • pp.117-121
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    • 2004
  • The patterns of diapause-associated proteins of silkworm eggs were analyzed by two-dimensional (2-D) gel electrophoresis. Among the hundreds of spots on the 2-D gels, at least two proteins were considered to be associated with diapause. A protein, spot 4, with an approximate molecular weight of 38 kDa and pI 6.1 was observed in the HCI-treated, cold-treated, and diapause eggs, respectively. Spot 4 was undetectable in unfertilized eggs and non-diapause eggs at two days after oviposition, suggesting that this protein may be associated with the entrance to diapause. A protein, spot 11, with an approximate molecular weight of 21 kDa and pI of 61 was detected in the unfertilized, HCl-treated, and cold-treated eggs, respectively, after oviposition by normal moths. In diapausing eggs, a protein corresponding to spot 11 was observed in 3-, 5-, and 30-day-old eggs, while the protein was not detected one-day-old eggs. The protein corresponding to spot 11 was not detected in unfertilized and non-diapause eggs obtained from subesophabeal ganglion (SG)-extirpated moths either. Spot 11 was also considered to be a diapause specific protein, which occurred at only early embryonic stage under the control of diapause-downregulated gene.

Scolopendrasin I: a novel antimicrobial peptide isolated from the centipede Scolopendra subspinipes mutilans

  • Lee, Joon Ha;Kim, In-Woo;Kim, Mi-Ae;Yun, Eun-Young;Nam, Sung-Hee;Ahn, Mi-Young;Lee, Young Bo;Hwang, Jae Sam
    • International Journal of Industrial Entomology and Biomaterials
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    • v.31 no.1
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    • pp.14-19
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    • 2015
  • In a previous report, we identified several candidate antimicrobial peptides through de novo RNA sequencing of the centipede Scolopendra subspinipes mutilans. Here, we identify and characterize one of these peptides, Scolopendrasin I. We identified the centipede antimicrobial peptide Cecropin from the centipede transcriptome using an SVM algorithm, and subsequently analyzed the amino acid sequence for predicted secondary structure using a GOR algorithm. We identified an alpha helical region of Cecropin and named it Scolopendrasin I. We then assessed antimicrobial and hemolytic activity of Scolopendrasin I. Scolopendrasin I showed antimicrobial activity against various microbes, including antibiotic-resistant Gram-negative bacteria, in a radial diffusion assay. Scolopendrasin I had potent antibacterial activity against acne-associated microbes in a colony count assay and showed no hemolytic activity in a hemolysis assay. In addition, we confirmed that Scolopendrasin I bound to the surface of bacteria via a specific interaction with lipoteichoic acid and lipopolysaccharide, two components of bacterial cell membranes. In conclusion, the results presented here provide evidence that this is an efficient strategy for antimicrobial peptide candidate identification and that Scolopendrasin I has potential for successful antibiotic development.