• Journal of the Korean Chemical Society
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• v.30 no.3
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• pp.312-319
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• 1986

Dodecyltrimethylammonium bromide (DTAB) was examined as a counter-ion in the investigation of capacity factor and separation of aromatic carboxylic acids on alkyl-modified silica (ODS) column as a stationary phase by ion-pair chromatography on reversed-phase system. The capacity factor of samples was influenced by the several factors such as a concentration of counter-ion as well as kinds and concentration of electrolyte, concentration of methanol in mobile phase and kinds and position of functional group in sample molecule. Some mixtures of samples were able to be separated under optimum condition.

• IMMUNE NETWORK
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• v.10 no.1
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• pp.5-14
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• 2010

Background: There have been several reports describing the capability of ginseng extracts as an adjuvant. In this study, we tested if ginsan, a polysaccharide extracted from Panax ginseng, was effective in enhancing antibody response to orally delivered Salmonella antigen. Methods: Ginsan was treated before oral salmonella antigen administration. Salmonella specific antibody was determined by ELISA. mRNA expression was determined by RT-PCR. Cell migration was determined by confocal microscopy and flow cytometry. COX expression was detected by western blot. Results: Ginsan treatment before oral Salmonella antigen delivery significantly increased both secretory and serum antibody production. Ginsan increased the expression of COX in the Peyer's patches. Various genes were screened and we found that CCL3 mRNA expression was increased in the Peyer's patch. Ginsan increased dendritic cells in the Peyer's patch and newly migrated dendritic cells were mostly found in the subepithelial dome region. When COX inhibitors were treated, the expression of CCL3 was reduced. COX inhibitor also antagonized both the migration of dendritic cells and the humoral immune response against oral Salmonella antigen. Conclusion: Ginsan effectively enhances the humoral immune response to orally delivered antigen, mediated by CCL3 via COX. Ginsan may serve as a potent vaccine suppliment for oral immunization.

• Bulletin of the Korean Chemical Society
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• v.28 no.12
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• pp.2293-2298
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• 2007

In this study, a dithizone extraction technique involving purge & trap GC-MS was developed for the determination of methylmercury in biological samples, especially blood and fish. After alkaline digestion, methylmercury in biological samples was extracted into dithizone and back-extracted into aqueous sulfide solution. The extracted methylmercury was converted to the volatile ethyl derivative, purged and trapped onto a solid-phase collection medium, and then introduced into the GC-MS system. The determined MDLs of the established method were 0.9 ng·g?1 for biological samples and its accuracy and precision were found to be 93% and 3.8%, respectively. The method was validated by analysis of CRMs such as SRM 966, BCR 463 and IAEA 407 and all analytical results were within certified ranges with average RSDs of less than 6%. The analytical results of field-sampled fish also showed that the method can be successfully used as an alternative for commonly used distillation method followed by GC-CVAFS detection.

• Journal of the Korean Chemical Society
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• v.51 no.5
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• pp.412-417
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• 2007

A simple prepared paste electrode (PE) of DNA immobilized on a carbon nanotube was utilized for monitoring the antibacterial agent oxytetracycline (OTC), using square-wave anodic stripping voltammetry (SWASV) and cyclic voltammetry (CV). Given these conditions, SWASV and CV working ranges were observed within 1-10 ngL-1 OTC. In the SWASV and CV for OTC concentrations of 0.1 mgL-1, the relative standard deviations (n=15) were 0.068 and 0.067, respectively. At the optimized condition, the detection limit was found to be 0.4 ngL-1 OTC. This method was applied to the hatchery fish tissue.

• Korean Journal of Agricultural Science
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• v.16 no.1
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• pp.56-61
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• 1989

In the present paper, we intented to analyze and to compare the different seed dormancy of two legume plants, Albizzia and Cercis. We found two different types of seed dormancy: one type of dormancy in Albizzia seed was caused by the water impermeability, and other type of seed dormancy was found in Cercis and it was induced by inhibitors. We confirmed that the extract substances from Cercis seed seemed to have strong regulational functions on the inhibition of seed germination and primary root growth of lettuce seed.

• Proceedings of the Korean Geotechical Society Conference
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• 2008.03a
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• pp.1061-1064
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• 2008

A completion of settling process takes a lot of time for dredged materials of high moisture content, such as contaminated sludge, in landfill site. In general, additives (e.g. flocculants) are used for reducing settling time of such colloidal material, which results in the increase of sludge volume, and hence much space is required in landfill site. This study is to suggest alternative method in order to enhance the settling process of cohesive clayey soils. A number of gravitational sedimentation tests as well as electrokinetic experiments were conducted to investigate the variation of initial moisture content on the settling behaviour of clay slurry. Surface settlement, electric current and local voltage gradient were monitored during the experiment, and moisture content and soil pH were measured after the experiment. From the results, the application of electrokinetics was found to be effective in volume reduction (i.e. increase of settling velocity and decrease of final moisture content) by comparison with gravitational settling process.

• Proceedings of the Korean Geotechical Society Conference
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• 2007.09a
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• pp.465-477
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• 2007

Recently a number of research has been performed for the combination of new high technologies in order to find the solutions for various problems in civil engineering research field. Various high-tech measuring instruments have been applied to the geotechnical engineering field, and many attempts have also been performed for the more accurate measurements. Especially, the researches using image on the digital photogrammetry technique are gradually extending nowadays. The computer program developed using the digital photogrammetry technique and the case studies are explained and presented in this paper. Therefore, it could be considered that not only the measurement with high-accuracy but the disaster prevention should be available through the practical use of digital photogrammetry technique. The applicability would also be increased in the measurement of both the geotechnical engineering field as well as other research fields.

• Journal of Environmental Health Sciences
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• v.23 no.3
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• pp.91-101
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• 1997

The biosynthesis of phospholipid and the composition of fatty acid in C. ellipsoidea mitochondria treated with antibiotics(cycloheximide, nalidixic acid) during the culture analyzed. The growth of Chlorella and the contents of total lipid in mitochondria treated with antibiotics were lower than those of the control. The synthesis of PC (phosphatidylcholine) and PI(phosphatidylinostiol) were inhibited in the nalidixic acid treatment and also the contents of PC(phosphatidylcholine), PE (phosphatidylethanolamine), PG(phosphatidylglycerol) and PI(phosphatidylinositol) in the cycloheximide treatment were also inhibited. The major fatty acids utilized for the various phospholipids formation in each antibiotics treatment were analyzed stearic acid, myristic acid, palmitic acid, oleic acid, linoleic acid and linolenic acid at the late phase of the culture.

• Publications of The Korean Astronomical Society
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• v.30 no.3
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• pp.789-800
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• 2015

During the Joseon dynasty, the Gwansanggam (觀象監, The Royal Astronomical Observatory) was the royal administration in charge of Astronomy (天文), Geography (地理), Calendars (曆), Astronomical and meteorological observation (測候), and Timekeeping (刻漏). Of those affairs, the Astronomical Almanac compilation was most important. In this paper, we study the education and selection of astronomical officials in the 19th century during the Joseon dynasty, focusing on Astronomical Almanac officials. According to this study, their main area was calendar calculation, and they were involved in Astronomical Almanac compilation for nearly 40 years, until they resigned. In addition, they served as Samryeok-susulgwan (三曆修述官, Official for calendar calculation by the $Sh{\acute{i}}xi{\grave{a}}n$ calendar), and Ilgwa-gamingwan (日課監印官, Official printing supervisor). To undertake these core duties, an official had to pass several examinations and courses. The Gwansanggam (after Gwansangso) carried out the educational function for the officials. In particular, reserved officials of the astronomy division had to be educated starting with a trainee course.

• The Bulletin of The Korean Astronomical Society
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• v.39 no.2
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• pp.91.2-91.2
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• 2014

A near infrared wide-band high resolution spectrograph, immersion grating infrared spectrometer (IGRINS) has been jointly developed by the Korea Astronomy and Space Science Institute and the University of Texas at Austin. The compact white-pupil design of the instrument optics includes five cryogenic flat mirrors including a slit mirror, an input fold mirror, a dichroic mirror, and H&K camera fold mirrors. In this study, we introduce the optomechanical mount designs of the five cryogenic mirrors. In order to meet the structural stability and thermal requirements of the mount models, we conducted the design work with the aid of 3-dimensional computer modeling and the finite element analysis (FEA) method. We also present the actual fabricated parts and assemblies of the mounts and mirrors as well as their CAD models.