• Title/Summary/Keyword: Lee Sam

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Cloning, Sequencing and Characterization of Mitochondrial Control Region of the Domestic Silkwom, Bombyx mori

  • Lee, Jin-Sung;Kim, Ki-Hwan;Hoe, Hyang-Sook;Park, Jae-Heung;Kang, Seok-Woo;Lee, Sang-Han;Hwang, Jae-Sam
    • International Journal of Industrial Entomology and Biomaterials
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    • v.2 no.1
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    • pp.87-89
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    • 2001
  • The nucleotide sequence of the domestic silkworm (Bombyx mori) mitochondrial (mt) control region and its flanking genes was determined from PCR clones. The control region of the silkworm mt genome was located between the small ribosomal RNA gene and transfer RN $A^{Met}$. This 499 bp control region hale 95.4% A+T content. Extensive comparative analysis studies performed with similar control region of other insect genomes could not reveal a highly conserved region containing conserved motifs of animal mito-chondrial genome. The remarkable feature that found in this control region was the presence of tandem motifs containing nine repetitive sequences. The potential usefulness of this motif sequences for Bombyx species or their taxonomically related species is enhanced by its unique localization in the maternally inheritance mitochondrial molecule.e.

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Breeding of a New Silkworm Variety, Kumhwangjam, with a Sex-Limited Cocoon Color for Spring Rearing Season

  • Kang, Pil Don;Sohn, Bong Hee;Lee, Sang Uk;Kim, Mi Ja;Jung, I Yeon;Kim, Young Soon;Kim, Yung Dae;Lee, Heui Sam
    • International Journal of Industrial Entomology and Biomaterials
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    • v.9 no.1
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    • pp.89-93
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    • 2004
  • A new silkworm variety, Kumhwangjam, with a sex-limited cocoon color for spring rearing season is F$_1$ hybrid between Jam 309, a Japanese race bred from Y1/9153, and Jam 310, a Chinese race from Y1/8586. In the local adaptability test performed at 8 local areas in spring rearing season of 2003, major characteristics of Kumhwangjam were mainly similar to those of the check variety Kumokjam. The new silkworm variety, Kumhwangjam, showed a sex-limited cocoon color: yellow for female and white for male with elliptical cocoon shape. This sex-limited cocoon color can be used as a valuable marker for discrimination between female and male for various purposes.

Prevalence of bovine tuberculosis in dairy cattle in Korea from 1961 to 2004

  • Yoon, Hachung;Chung, Byung-Hyun;Yoon, Chong-Sam;Lee, Joo-Ho;Moon, Oun-Kyoung;Park, Seung-Yong;Lee, Won-Chang;Kim, Tae-Jong
    • Korean Journal of Veterinary Research
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    • v.48 no.1
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    • pp.49-52
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    • 2008
  • The trend in the prevalence of bovine tuberculosis (BTB) in Korean dairy cattle was investigated in relation to test programs used between 1961 and 2004, during which a total of 8,961,061 dairy cows were tested and 10,248 confirmed to have BTB. The annual prevalence increased in the late 1960s, then decreased during the 1970s and 1980s, and started to increase again from the late 1990s. It seemed that the prevalence varies according to the different test program used. The prevalence of BTB was higher when the tests were performed with heat-concentrated synthetic medium (HCSM) or purified protein derivative (PPD) tuberculin alone compared to that when using combined HCSM and PPD tuberculin testing.

A Study on Prediction of Bed Changes in the Imjin River Using the HEC-6 Model (HEC-6 모형을 이용한 임진강 하상변동 예측에 관한 연구)

  • Lee, Nam-Joo;Hwang, Seung-Yong;Lee, Sam-Hee
    • Proceedings of the Korea Water Resources Association Conference
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    • 2006.05a
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    • pp.1566-1570
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    • 2006
  • 이 연구는 하상변동 수치모형을 적용하여 임진강의 장기간 하상변동과 그에 따른 하천 수위 변화의 예측, 안정하상 검토, 하상변동이 계획홍수위에 미치는 영향, 그리고 하상준설이 수위 및 하상변동에 미치는 영향의 검토를 통해 해당 하천에서 하상변동 특성의 파악에 필요한 자료 및 중.장기 수방대책 수립을 위한 기본 자료를 제공할 목적으로 수행하였다. 임진강 하상변동의 예측에 적절한 일차원 정상 하상변동모형으로 HEC-6 모형을 선정하였다. 모형적용을 위한 현황분석으로 하상토 특성 자료를 수집.분석하였으며, 지형자료에 대한 분석도 수행하였다. 그리고 모형의 적용영역 내에 위치한 각 수위표 지점의 수리.수문자료를 수집하여 분석하였다. HEC-6 모형의 보정을 고정상 보정과 이동상 보정으로 구분하여 수행하였다. 고정상 보정에서는 설정한 조건에 적합한 Manning계수의 결정이 곤란하여 하천의 지형자료와 하상토 자료 등을 고려하여 구간별로 적정한 Manning 계수 값을 선정하였다. 이동상 보정을 통해 HEC-6 모형에서 사용하는 유사량 산정공식 중 하류경계에서 상류로 약 8 km 구간에 대한 하상상승 현상을 가장 잘 보여주는 유사량 공식인 Madden(1963)이 수정한 Laursen(1958) 공식을 선택하였다. 하천망 유사이동 수치모형인 HEC-6 모형을 사용하여 20년간의 장기하상변동을 수치모의하였다. 임진강의 최심하상고 변동량 예측결과 상류경계로부터 약 52 km까지는 하상저하가 지배적이며, 중 하류는 하상상승이 지배적인 것으로 나타났다. 연구 대상구간에서 대체로 퇴적에 의한 하상상승이 예상되었다.

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Relative Analysis of Computed Discharge and Actual Survey Discharge in Nak Dong Basin (낙동강 유역 주요지점에서의 환산유량과 실측유량의 비교분석)

  • Hwang, Su-Jin;Kim, Sam-Eun;Lee, Ki-Sung;Lee, Jin-Won;Jung, Sung-Won
    • Proceedings of the Korea Water Resources Association Conference
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    • 2009.05a
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    • pp.1072-1077
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    • 2009
  • 귀중한 수자원을 효율적으로 관리하고 홍수 피해를 저감하고 환경친화적인 수자원의 역할을 제대로 자리매김하기 위하여 무엇보다 필요한 사항은 신뢰성 있는 유량자료를 기초자료로서 확보하는 것이다. 하지만 과거 낙동강 유역의 유량 자료는 유량측정성과와 수위자료의 불확실성으로 인하여 환산 유량 자료는 근본적인 한계가있을 뿐만 아니라 시시각각으로 변하므로 불확실성이 높은 특성을 지니고 있다. 따라서 본 연구에서는 유량조사사업단 설립 이후에 측정된 유량자료의 정확도를 분석하기 위하여 낙동강 유역의 가장 대표적인 수위관측지점인 낙동, 왜관, 진동 지점에 대하여 수위-유량관계곡선식의 정확도를 분석하고, 작성된 수위-유량관계곡선식을 이용한 환산유량과 실측유량의 유량값을 비교 분석하므로서 유량자료에 대한 신뢰성을 검증하고 문제점을 분석하고자 하였다. 유량조사사업단에서 실시하고 있는 주요지점들의 환산유량의 정확성은 낙동강홍수통제소의 지속적인 T/M자료의 관리로 인한 정확도의 향상으로 인하여 수위-유량관계곡선식은 물론 이에 따른 환산 유량이 높은 신뢰성을 갖는 것으로 나타났다.

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Isolation and Characterization of Psacotheasin, a Novel Knottin-Type Antimicrobial Peptide, from Psacothea hilaris

  • Hwang, Jae-Sam;Lee, June-Young;Hwang, Bo-Mi;Nam, Sung-Hee;Yun, Eun-Young;Kim, Seong-Ryul;Lee, Dong-Gun
    • Journal of Microbiology and Biotechnology
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    • v.20 no.4
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    • pp.708-711
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    • 2010
  • We report the isolation and characterization of a novel knottin-type antimicrobial peptide from the yellow-spotted long-horned beetle Psacothea hilaris. A cDNA encoding a 56-mer knottin-type propeptide was identified and its predicted molecular mass and pI were 5.92 kDa and 8.28, respectively. A 34-mer mature peptide was also selected and named herein as psacotheasin. The antimicrobial activity of chemically synthesized psacotheasin against human bacterial pathogens was subsequently investigated. The results showed that psacotheasin exerted potent activities against both Gram-positive and Gram-negative bacterial strains. The present study suggests that psacotheasin can be applied to develop novel therapeutic agents.

Inundation Analysis Considering Water Waves and Storm Surge in the Coastal Zone (연안역에서 고파랑과 폭풍해일을 고려한 침수해석)

  • Kim, Do-Sam;Kim, Ji-Min;Lee, Gwang-Ho;Lee, Seong-Dae
    • Journal of Ocean Engineering and Technology
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    • v.21 no.2 s.75
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    • pp.35-41
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    • 2007
  • In general, coastal damage is mostly occurred by the action of complex factors, like severe water waves. If the maximum storm surge height combines with high tide, severe water waves will overflow coastal structures. Consequently, it can be the cause of lost lives and severe property damage. In this study, using the numerical model, the storm surge was simulated to examine its fluctuation characteristics at the coast in front of Noksan industrial complex, Korea. Moreover, the shallow water wave is estimated by applying wind field, design water level considering storm surge height for typhoon Maemi to SWAN model. Under the condition of shallow water wave, obtained by the SWAN model, the wave overtopping rate for the dike in front of Noksan industrial complex is calculated a hydraulic model test. Finally, based on the calculated wave-overtopping rate, the inundation regime for Noksan industrial complex was predicted. And, numerically predicted inundation regimes and depths are compared with results in a field survey, and the results agree fairly well. Therefore, the inundation modelthis study is a useful tool for predicting inundation regime, due to the coastal flood of severe water wave.

Morphology Control of ZnO Nanorods on ITO Substrates in Solution Processes (습식공정 기반 ITO 기판 위 산화아연 나노로드 모폴로지 제어)

  • Shin, Kyung-Sik;Lee, Sam-Dong;Jeong, Soon-Wook;Lee, Sang-Woo;Kim, Sang-Woo
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.22 no.11
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    • pp.987-991
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    • 2009
  • We report growth of vertically well-aligned zinc oxide (ZnO) nanorods on indium-tin oxide (ITO)/glass substrates using a simple aqueous solution method at low temperature via control of the ZnO seed layer morphology. ZnO nanoparticles acting as seeds are pre-coated on ITO-coated glass substrates. by spin coating to control distribution and density of the ZnO seed nanoparticles. ZnO nanorods were synthesized on the seed-coated substrates in a dipping process into a main growth solution. It was found that the alignment of ZnO nanorods can be effectively manipulated by the spin-coating speed of the seed layer. A grazing incidence X-ray diffraction pattern shows that the ZnO seed layer prepared using the higher spin-coating speed is of uniform seed distribution and a flat surface, resulting in the vertical growth of ZnO nanorods aligned toward the [0001] direction in the main growth process.

Synchronization in Simulation and On-line Feedback Control for Mobile Robots Using The MSRDS (MSRDS를 이용한 모바일 로봇의 시뮬레이션 동기화 및 온라인 피드백 제어)

  • Lee, Yoon-Sub;Choi, Sang-Ho;Lee, Young-Sam;Kim, Jin-Geol
    • Proceedings of the KIEE Conference
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    • 2008.10b
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    • pp.93-94
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    • 2008
  • 로봇을 개발함에 있어 많이 겪는 어려움 중 하나는 열악한 시뮬레이션 환경 및 소프트웨어 개발이다. Microsoft사(社)의 MSRDS(Microsoft Robotics Developer Studio)는 3D시뮬레이션 환경과 로봇 구동을 위한 각종 표준화된 서비스들을 지원함으로써 이러한 문제점들을 해결할 수 있다. 본 논문에서는 원자력 발전소의 중 저준위 폐기물 저장창고 검사 로봇을 대상으로 MSRDS를 이용하여 3D 시뮬레이션 및 온라인 피드백 제어를 수행하였다. 로봇은 사륜으로 이루어져 있으며, 주행 환경 확인을 위한 카메라, 장애물 감시용 LRF(Laser Range Finer)센서, 주변 환경 감지를 위한 온도센서, 습도센서 및 MSRDS가 장착된 산업용 3D가 탑재되어 있다. MSRDS의 제어신호는 이벤트로 생성되어 3D 시뮬레이션 렌더링 시간과 동기화되며, Brick 서비스를 통해 전송된다. 피드백은 10ms 주기로 LRF 센서, Motor 엔코더 값을 받아 3D 시뮬레이션에 반영된다. 본 논문에서는 MSRDS의 시뮬레이션 및 실제 로봇과의 동기화 방식을 제시하며 구동 실험으로 검증하였다.

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Water Quantity and Quality Management Through A Multiple Reservoir System (수량(水量) 및 수질(水質)을 고려(考慮)한 저수지군(貯水池群)의 종합관리(綜合管理))

  • Ko, Seok Ku;Kim, Soo Sam;Lee, Kwang Man;Lee, Ki Jong
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.12 no.1
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    • pp.123-130
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    • 1992
  • The quantity point has been considered prior to the quality point in the water resources development and management. However, the quality problems have become as important as quantity problems due to the industrialization and civilization. This paper presents a methodology of the integrated management of multiple reservoir systems by considering both water quantity and quality problems. It also presents a long-term forecasting technique of reservoir water quality by using the developed phosphorous model. The methodology was applied to the Han river reservoirs operation, in which five major reservoirs were considered. The result shows that the spatially and temporally distributed water quantity and quality resources can be utilized by meeting all the required constraints.

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