• Title/Summary/Keyword: Lee Ha-Bok

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Effect of floor types (slat vs. litter) of group housing systems on sow behavior and environmental levels (모돈 군사 사육의 깔짚과 슬랏바닥의 행동 및 환경 수준 평가 비교)

  • Yang, Ka Young;Ha, Jae Jung;Kwon, Kyeong Seok;Kim, Jong Bok;Jang, Dong Hwa;Lee, Jun Yeob;Kim, Jung Kon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.8
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    • pp.388-394
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    • 2020
  • This study was undertaken to evaluate and compare the status of animal welfare, environmental level, and sow behavior, by including two farmhouses with sow group housing but having different floor types, viz., litter floor and slat floor. Animal welfare level was evaluated for body and vulval wounds of sows. The environmental level was measured for a total of 4 months, assessing the concentrations of H2S, NH3 and CO2 inside the pig house, once a month. Results of this study indicate that the level of animal welfare, with respect to body and vulval wounds, was better in the concrete slat floor farmhouse (H) than in the litter floor farmhouse (K). Environmental levels obtained (in ppm) were: H2S (H, 1.0; K, 0.0), NH3 (H, 45.4; K, 1.3), and CO2 (H, 1102.3; K, 258.8), indicating higher levels in the H than in K farmhouse. Aggressive behavior was mainly encountered in the H farmhouse. These results indicate that the same group housing system, but with different flooring, results in changes pertaining to aggressive behavior and environmental levels. It is therefore necessary to study the behavior changes, welfare, and environmental levels while deliberating different floor types.

Comparison of Physical & Histological Change of Alloplastic Implants after Implantation in Rat (흰쥐에 삽입된 인공성형삽입물의 생체내 물리, 조직학적 변화의 비교)

  • Kim, Sung Nam;Noh, Bok Kyun;Kim, Eui Sik;Hwang, Jae Ha;Kim, Kwang Seog;Lee, Sam Yong;Cho, Bek Hyun
    • Archives of Plastic Surgery
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    • v.33 no.2
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    • pp.219-224
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    • 2006
  • Augmentation rhinoplasty is one of the most popular aesthetic procedure in Asians. Numerous alloplastic implants have been used until now, but no accurate comparative analysis about the implant materials has been reported yet. This study in animal model was designed to determine the safety and effectiveness of various implant materials in augmentation rhinoplasty. The $15{\times}15{\times}2mm$ sized square shaped plate of $Gore-Tex^{(R)}$, silicone rubber, and $15{\times}15{\times}1.5mm$ sized $Medpor^{(R)}$ were implanted under panniculus carnosus of the abdomen wall of rat. And tissue specimens including the implant and surrounding soft tissue were obtained by en bloc excision in 6 months after implantation. The implants were estimated in weight and volume, and also the specimens were examined grossly and microscopically. The results revealed that increase of average weight 26.9%, decrease of average volume 55.4% in $Gore-Tex^{(R)}$ implant, increase of each average weight and volume 62.6%, 8.7% in $Medpor^{(R)}$ implant and very slight increase of both average weight and volume 4.7%, 1.1% in silicone rubber implant. Grossly, the $Gore-Tex^{(R)}$ was deformed, $Medpor^{(R)}$ was strongly adherent to surrounding soft tissue and the silicone rubber was well encapsulated and easily peeled off. Microscopically, silicone rubber showed foreign body reaction slightly and there were no inflammatory responses in all alloplastic implants. In our study, silicone rubber showed very proper alloplastic features for augmentation rhinoplasty due to causing no inflammatory response, no physical change, and no deformity.

Correction of Recurrent Ingrowing Toenail Using Partial Matricectomy and Paronychial Advancement Flap (부분기질절제술과 발톱주위전진피판을 이용한 재발성 내증식발톱의 교정)

  • Kim, Eui Sik;Kim, Sung Nam;Noh, Bok Kyun;Hwang, Jae Ha;Kim, Kwang Seog;Lee, Sam Yong
    • Archives of Plastic Surgery
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    • v.33 no.3
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    • pp.347-352
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    • 2006
  • Ingrowing toenail has been treated conventionally by various surgical treatment modlities. However, none of these procedures are perfect to achieve esthetic results with low cost, recurrence, and complication rates. Although mild cases can be treated conservatively, in severe recurrent cases, surgical treatment is preferred. The purpose of this study is to evaluate the effectiveness and safety of using partial matricectomy and paronychial advancement flap for correction of recurrent ingrowing toenail. Eight patients suffering from recurrent ingrowing toenail were operated using partial matricectomy and paronychial advancement flap in our department from August 2004 to August 2005. The number of recurrent episodes ranged from 2 to 5. With digital nerve block, the excision area was marked on nailplate and inflammed lateral nail fold. The horizontally designed paronychial flap considering defect size was effective for wide exposure of the neighboring germinal matrix, which is later to be completely excised or cauterized because remnant germinal matrix made postoperative spicule formation. The nailplate on the affected side was removed about 3-5 mm width longitudinally, being cautious not to damage the proximal dorsal nail fold. The same width of the sterile matrix including germinal matrix was excised longitudinally with inflammed granulation tissue and partial lateral nail fold on the affected side. The subcutaneously elevated paronychial flap was advanced to cover the defect and was anatomically sutured with 5-0 nylon sutures. We evaluated our procedure's effectiveness by examining some factors such as cosmetic results, recurrence, spicule formation, postoperative pain and duration of return to normal activities. In eight patients, no complication was observed such as recurrence, severe pain and spicule formation except for 1 case of infection. Postoperative discomfort was minimal. Average duration of return to normal activities was 12 days. Cosmetically the results were acceptable in all patients. The partial matricectomy and paronychial advancement flap for correction of recurrent ingrowing toenail were found to be technically simple, efficient procedure with a relative low recurrence rate and better cosmetic results.

Quality Characteristics and Antioxidant Activities of Grifola frondosa Tea with Different Pre-treatments (잎새버섯차의 전처리방법에 따른 품질특성 및 항산화활성)

  • Shin, Bok-Eum;Lee, Ja-Young;Kim, Jeong-Han;Choi, Jong-In;Ha, Tai-Moon;Jeong, Gu-Hyun
    • Journal of Mushroom
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    • v.18 no.3
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    • pp.254-259
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    • 2020
  • Grifola frondosa is a promising new kind of cultivated mushroom owing to its excellent taste and functionality. However, more research is required to determine its value. In this study, the effects of pretreating Grifola frondosa tea by drying, steaming, and warming, on quality characteristics and antioxidant activities were analyzed. There was no difference in sugar, soluble solid, and nitrogen content between dried and steamed tea. The color of the warm pretreated tea was the darkest, and the amino acid content was 462.9 mg/L, which was 1.8 times higher than that of the other pretreatments. Moreover, the warm pretreated tea had the highest total polyphenol and flavonoid content, 14.6 mg/g and 2.2 mg/g, respectively. DPPH and ABTS radical scavenging activities significantly increased with the increase of phenolic compounds. Warming pre-treatment slightly increased the taste preference to 7.0. In conclusion, warming Grifola frondosa before drying was confirmed to improve the extraction of nutrients, antioxidants, and taste preference.

Color Analysis of Forehead Flap and Full Thickness Skin Graft in Facial Reconstruction (이마피판술과 후이개부 전층피부이식술을 이용한 안면부 연부조직결손 재건 후 피부 색상의 비교)

  • Cho, Sung Hoo;Yoo, Sung In;Noh, Bok Kyun;Kim, Eui Sik;Hwang, Jae Ha;Kim, Kwang Seog;Lee, Sam Yong
    • Archives of Plastic Surgery
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    • v.35 no.1
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    • pp.36-41
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    • 2008
  • Purpose: For facial reconstruction, skin color match is crucial to achieve great aesthetic result. Forehead flap and full thickness skin graft have been used for many years to reconstruct facial defect. Their results are aesthetically valuable with remarkable resemblance and harmony of the skin color between donor and recipient sites. The purpose of this study is to evaluate and compare the aesthetic outcome of the two methods as the analysis of skin color match. Methods: From January 1995 to December 2005, ten forehead flaps and ten full thickness skin grafts were performed. The reconstructed areas of forehead flaps were five noses and five eyelids. Recipient sites of full thickness skin grafts were seven eyelids, two noses and one forehead. In order to obtain the objective validity, the skin color of flap(or graft) and the recipient sites were measured by chromameter. The skin colors were quantified according to a three-dimensional coordinate system used in chromameter, L*(brightness), a*(redness), and b*(yellowness).Results: There was no significant color difference between forehead flap site and adjacent skin in all color values. On the other hand, the L* and b* values of graft sites were significantly lower than those of the adjacent skins. The a* values of graft sites were higher than those of the adjacent skins. Conclusion: This study reveals that skin color match of forehead flap is greater than that of full thickness skin graft. As forehead flap has adequate volume and great color match, it can be useful to reconstruct deep facial defect such as nasal defect. On the other hand, full thickness skin graft can be used for superficial defect like partial eyelid defect.

Characteristics of a new variety 『Gonji10ho』 in Neolentinus lepideus (잣버섯 신품종 『곤지10호』 특성)

  • Jeon, Dae-Hoon;Jang, Myoung-Jun;Ha, Tai-Moon;Lee, Yun-Hae;Choi, Jong-In;Ju, Young-Cheul;Yoo, Young-Bok
    • Journal of Mushroom
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    • v.11 no.4
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    • pp.249-253
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    • 2013
  • 'Gonji10ho', a new variety of Neolentinus lepideus, was bred by mating two monokaryotic strains isolated from 'GMNL66037' and 'GMNL66014' in Mushroom Research Institute, Gyonggi-Do ARES in 2012. The optimum temperature for the mycelial growth of 'Gonji10ho' was $29^{\circ}C$ on PDA medium and that for the primordia formation and the growth of fruiting body of 'Gonji10ho' was $20{\pm}1^{\circ}C$. In bottle cultivation of 'Gonji10ho', the period of spawn running was around 35 days at $22{\pm}1^{\circ}C$ and the period from scratching of inoculum to harvesting was 27 days. 'Gonji10ho' had thicker pileus and stipe than control variety('Solhyang'). The number of available stipe was fewer than that of control variety. During the cultivation of 'Conji10ho', the occurrence rate of Trichoderma spp. was lower than that in control variety. In bottle cultivation, the yield of fruiting bodies of 'Gonji10ho', which was 38% higher than that of control variety, was 69.6 g/900 ml bottle, and in bag cultivation, that of 'Gonji10ho', which was 10% higher than that of control variety, was 114.2 g/1 kg bag. In physical characteristics, the strongness and brittleness of the pileus of 'Gonji10ho' were higher than those of control variety. When considered in the period available for sale, the shelf life of 'Gonji10ho', which was 5 days longer than that of control variety, was 34 days.

Measurement of Dust Concentration in a Mechanically Ventilated Broiler House and Analysis of Dust Generation from Ground Beds (강제환기식 계사의 공기 중 분진 농도 측정 및 바닥재로부터의 분진 발생량 분석)

  • Kwon, Kyeong Seok;Jo, Ye Seul;Lee, In Bok;Ha, Tae Hwan;Hong, Se Woon
    • Journal of The Korean Society of Agricultural Engineers
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    • v.56 no.6
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    • pp.31-43
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    • 2014
  • Confined and mechanically ventilated broiler house has been recently come into wide use to increase productivity and control rearing conditions. However, high dust concentration inside facility can threaten respiratory welfare of farmers and broilers. In Korea, there is no regulation in terms of air quality control inside agricultural facilities and sufficient data is not available. To cope with these, periodic monitoring of inhalable and respirable dust concentration were conducted according to season, broiler's activity and specific events such as shipment work for broilers in mechanically ventilated broiler house. Chamber experiment was also designed to analyze mechanism of dust generation from ground beds according to water contents and surrounding wind environment. Results showed that significant increase of inhalable (p=0.002) and respirable (p=0.03) dust were observed when activity of broilers was high according to entrance of the workers. Even shipment work for matured broilers, high level of dust were observed; inhalable dust was exceeded the threshold limit over maximum 303 % and respirable dust was over maximum 1,550 %, implying that acute respiratory symptoms could be manifested for the workers. From the chamber experiment, critical water contents for interrupting of dust generation were measured; about 45 % for inhalable dust and about 50 % for respirable dust. These results can be a trigger for designing plan of dust control however it still needs consideration of various environmental conditions, hygiene problems, etc.

Studies on the Improvement of Roasting Condition of Sesame Seeds for Producing Seed Season and Oil (볶음 및 참기름용 참깨 가공방법 개선에 관한 연구)

  • Park, Chang-Hwan;Choi, Kyoung-Jin;Shim, Kang-Bo;Ha, Tae-Joung;Lee, Myoung-Hee;Hwang, Joung-Dong;Pae, Suk-Bok;Park, Kum-Young;Baek, In-Youl
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.56 no.3
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    • pp.205-211
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    • 2011
  • This study was carried out to find optimum roasting condition of sesame seeds for making seed season and oil treated with different temperatures and time intervals. Sesame seeds with 17~18% of moisture content were treated under fixed and changed roasting temperature conditions. The fixed temperatures are ranged from 160 to $240^{\circ}C$ with $20^{\circ}C$ intervals. The changed temperatures were treated at low(160 and $180^{\circ}C$) and medium(200 and $220^{\circ}C$) for 10 minutes, and at high(220 and $240^{\circ}C$) for 3 minutes. Meanwhile, roasting times were 20-30 minutes longer under low temperature condition and 3-5 minutes shorter under high temperature condition. The optimum roasting temperature and time were determined as $220^{\circ}C$ and 3 minutes for producing seed season, and as $220^{\circ}C$ and 5 minutes for sesame oil, respectively, in roasting with small quantity of sesame seeds. On the other hand, in the large scale roasting condition, those showed $240^{\circ}C$ and 15 minutes in for producing seed season, and $280^{\circ}C$ and 10 minutes and $260^{\circ}C$ and 15 minute for producing seed season in white-colored sesame variety and black-colored sesame variety, respectively.

Estimation of Oil Yield of Perilla by Seed Characteristics and Crude Fat Content

  • Oh, Eunyoung;Lee, Myoung Hee;Kim, Jung In;Kim, Sungup;Pae, Suk-Bok;Ha, Tae Joung
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.63 no.2
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    • pp.158-163
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    • 2018
  • Perilla (Perilla frutescens var.frutescens) is an annual plant of the Lamiaceae family, mainly grown for obtaining oil by press extraction after roasting the seeds. Oil yield is one of its important traits, but evaluating this yield is time-consuming, requires many seeds, and is hard to adjust to pedigrees in a breeding field. The objective of this study was to develop a method for selecting high-oil-yield lines in a breeding population without oil extraction. Twenty-three perilla cultivars were used for evaluating the oil yield and seed traits such as seed hardness, seed coat thickness, seed coat proportion and crude fat. After evaluation of the seed traits of 23 perilla cultivars, the ranges of oil yields, seed hardness, seed coat thickness, seed coat proportion, 100-seed weight, and crude fat were 24.68-38.75%, 157-1166 gf, $24-399{\mu}m$, 15.4-41.5%, 2.79-6.69 g, and 33.0-47.8%, respectively. In an analysis of correlation coefficients, the oil yield negatively correlated with seed length, seed width, the proportion of seed coat, seed hardness, and 1000-seed weight, but positively correlated with crude fat content. It was observed that as the seed coat proportion increased, the seed coat thickness, hardness, and 1000-seed weight also increased. Multiple linear regression (MLR) was employed to find major variables affecting the oil yield. Among the variables, traits crude fat content and seed coat proportion were assumed to be indirect parameters for estimating the potential oil yield, with respect to a significant positive correlation with the observed oil yield ($R^2=0.791$). Using these two parameters, an equation was derived to predict the oil yield. The results of this study show that various seed traits in 23 perilla cultivars positively or negatively correlated with the oil yield. In particular, crude fat and the seed coat proportion can be used for predicting the oil yield with the newly developed equation, and this approach will improve the efficiency of selecting prominent lines for the oil yield.

Extensive Hepatic Uptake of Pz-peptide, a Hydrophilic Proline-Containing Pentapeptide, into Isolated Hepatocytes Compared with Colonocytes and Caco-2 Cells

  • Shin, Tae-Ha;Lee, Pung-Sok;Kwon, Oh-Seung;Chung, Youn-Bok
    • Archives of Pharmacal Research
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    • v.26 no.1
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    • pp.70-75
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    • 2003
  • The objective of the present study was to investigate the uptake process of 4-Phenylazobenzoxycarbonyl-Pro-Leu-Gly-Pro-D-Arg (Pz-peptide), a hydrophilic and collagenase-labile pentapeptide, by isolated hepatocytes. For comparison, the uptake of Pz-peptide by Caco-2 cells and colonic cells, two known paracellular routes of Pz-peptide, was also evaluated. A simple and sensitive reversed-phase HPLC assay method using UV detection has been developed. The coefficient of variation for all the criteria of validation were less than 15%. The method was, therefore, considered to be sutable for measuring the concentration of Pz-peptide in the biological cells. Pz-peptide was extensively uptaked into hepatocytes. The initial velocity of Pz-peptide uptake assessed from the initial slope of the curve was plotted as Eadie-Hofstee plots. The maximum velocity ($V_{max}$) and the Michaelis constant ($K_m$) were 0.190$\pm$0.020 $nmol/min/10^6$ cells and 12.1$\pm$3.23 $\mu$M, respectively. The permeability-surface area product ($PS{influx}$) was calculated to be 0.0157 ml/min/10^6$ cells. $V_{max}$ and $K_m$ values for Caco-2 cells were calculated to be 6.22$\pm$0.930 pmol/min/10^6$ cells and 82.8$\pm$8.37 $\mu$M, respectively, being comparable with those of colonocytes (6.04$\pm$1.03 pmol/min/10^6$ cells and 87.8$\pm$13.2 $\mu$M, respectively). $PS_{influx}$ values for Caco-2 cells and colonocytes were calculated to be 0.0751 $\mu$l/min/10^6$ cells and 0.0688 $\mu$l/min/10^6$ cells, respectively. The more pronounced uptake of Pz-peptide by hepatocytes, when compared with Caco-2 cells and colonocytes, is probably due to its specific transporter. In conclusion, Pz-peptide, a paracellularly transported pentapeptide in the intestine and ocular epithelia, was uptaked into hepatocytes extensively. Although Pz-peptide is able to be uptaked into the Caco-2 cells and colonocytes, it is less pronounced when compared with hepatocytes. $PS_{influx}$ values of Caco-2 cells and colonocytes for unbound Pz-peptide under linear conditions were less than 0.4% when compared with that of hepatocytes.