• Title/Summary/Keyword: Lee Geun-sam

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A Nutritional Analysis of Chinese Red-headed Centipedes (Scolopendra subspinipes mutilans) from Different Regions of Korea (지역에 따른 국내산 왕지네(Scolopendra subspinipes mutilans (Arthropoda:Chilopoda))의 영양성분 및 유해물질 비교분석)

  • Kim, Sun Young;Lee, Kyeong Yong;Kim, Hong Geun;Hwang, Jae-Sam;Yoon, Hyung Joo
    • Journal of Life Science
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    • v.27 no.11
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    • pp.1308-1314
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    • 2017
  • The Chinese red-headed centipede Scolopendra subspinipes mutilans, a member of the arthropod class Chilopoda, is a traditional medicine used for the treatment of several allergic diseases, such as atopy. S. subspinipes mutilans samples were collected from different regions of South Korea: Sancheong, Gyeongnam; Yeonggwang, Jeonnam and Jeju-do. The nutritional values of the centipedes were analyzed to extend the species' applications. The crude protein and fat contents of all samples were high and ranged from 54.9-55.8% and from 26.8-30.6%, respectively. Essential amino acids were present; lysine was the most common and accounted for 3.4-3.6% of the essential amino acids. Glutamic acid, which assists in improving concentration, memory and other cognitive abilities, was the most common non-essential amino acid at 6.8-7.1%. It had a similar content percentage in all three regions' samples. Additionally, unsaturated fatty acids were present, and oleic acid, which prevents cancer and cardiac disease, was the most common at 41.3-48.6% of each sample. Mercury, a hazardous substance, was detected at a range of 0.08-0.11 mg/kg in all samples; the amount was lower than the standard food allowance. Additionally, no pathogenic microorganisms, such as Escherichia coli and Salmonella spp., were detected. There were no significant differences between the nutritional factors of the S. subspinipes mutilans samples from the three regions. Based on the nutritional analysis, Chinese red-headed centipedes have the potential to be food and medicinal ingredients due to their proteins, essential amino acids, unsaturated fatty acids and nutritive capacities.

Distribution and Species Composition of Phytoplankton in the Southern Waters of Korea and their Relation to the Character of Water Masses (한국남해의 식물플랑크톤의 분포와 수괴특성)

  • PARK Joo-Suck;LEE Sam-Geun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.23 no.3
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    • pp.208-214
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    • 1990
  • Phytoplankton samples for this study were collected by Van Dorn Sampler at 28 oceanographic stations in the southern waters of Korea in February, April and August, 1988 to characterize the distribution and species composition of phytoplankton and their relation to the movement of water masses. During the study periods, phytoplankton standing crops in the southern waters were maximum in April and minimum in February. When the geographical distribution and abundance of the phytoplankton were considered, the highest standing crops were found near the front zone formed between Cheju Island and Tsushima Island. Therefore, their abundance depends ell the location of the front zone. On the other hand, there were relatively low standing crops in the open sea off the coastal area from the front zone in February and August. The cause of the unusal low standing crops found in August at the station 205/01, the central part of the coastal area of the southern waters where the largest standing crops occurred in February and April, seems to be due to the intrusion of Tsushima warm waters. This is because the waters at the station have high temperature and salinity, poor dissolved oxygen and nutrient salts, which are identical to the characteristics of proper Tsushima warm waters. It seems that these warm waters may affect the poor productivity in this area. Seventy-two species in thirty-three genera of the phytoplankton were indentified from the samples. Among them, 61 species in 27 genera belong to diatoms and 12 species in 7 genera to dinoflagellates. The largest number of phytoplankton species occurred in August while the largest number of the tells in April. Predominant species were Eucampia zodiacus in February, Skeletonema costatum and Chaetoceros curvisetus in April and Chaetoceros affinis in August. Eucampia zodiacus which was dominant in February and April seems to be a indicator of the southern coastal waters of Korea and mixed waters west of Cheju Island.

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Evaluation of Strain Distribution and Pullout Strength based on Width and Horizontal Spacing of Geosynthetic Strip (띠형 섬유보강재의 폭과 설치간격에 따른 변형률 분포 및 인발강도 특성 평가)

  • Lee, Kwang-Wu;Cho, Sam-Deok;Han, Jung-Geun;Hong, Ki-Kwon
    • Journal of the Korean Geosynthetics Society
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    • v.11 no.2
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    • pp.39-47
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    • 2012
  • This paper describes large-scale pullout test results of geosynthetic strip, which can be applied in reinforced earth wall with block-type wall facing. The pullout tests are conducted to evaluate the strain distribution, the induced pullout force and the pullout strength. The maximum pullout force is appeared regardless of reinforcement width and normal stress when end displacement is less than 15 mm. The pullout behavior based on horizontal spacing of reinforcement was similar in relationship between pullout force and end displacement. The strain distribution and pullout force distribution of the geosynthetic strip are concentrated in the front part of reinforcement, and it appeared clearly in higher normal stress condition This means that the pullout behavior of geosynthetic strip is affected by the bond between soil and friction resistance reinforcement according normal stress. Therefore, the pullout resistance design is reasonable when pullout behavior of geosynthetic strip should be evaluated by effective length considering tensile characteristic.

Immunohistochemical Study on the Hair Growth Promoting Effect of Yonnyuniksoogobon-dan

  • Jeong, Chun-Geun;Park, Min-Hee;Seong, Ju-Won;Lee, Hyun-Sam;Park, Seong-Kyu;Kim, Sun-Yeou;Kim, Yoon-Bum;Jung, Hyuk-Sang;Sohn, Nak-Won;Sohn, Young-Joo
    • The Journal of Korean Medicine
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    • v.29 no.5
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    • pp.77-89
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    • 2008
  • Objectives : This study evaluates the hair growth promoting effect of Yonnyuniksoogobon-dan on shaved C57BL6 mice. Methods : Yonnyuniksoogobon-dan was administered orally (Group I) and both orally and by skin application (Group II) once a day for 12 days. The experimental groups were compared to Control, which was orally administered physiological saline solution. Hair regrowth was photographically and histologically determined during the experimental period. The levels of hair growth cycle related factors (EGF, TGF-${\beta}$1) and vascular factors (VEGF, iNOS) were also determined by immunohistochemistry. On gross observation of hair growth, both Group I and Group II shaved C57BL6 mice showed accelerated hair regrowth. Results : The hair regrowth index of Group I increased significantly from 7 days (P<0.05) to 12 days (P<0.01) after shaving and that of Group II was significantly higher at 12 days (P<0.05). On histological observation, both Group I and Group II demonstrated histological improvement and increases in the number and diameter of the hair follicles. EGF expression on the root sheath of hair follicles was up-regulated in both Group I and II. TGF-${\beta}$1 expression on the root sheath of hair follicles was unchanged in both Group I and II. VEGF expression in the tissues surrounding hair follicles was up-regulated in both groups. iNOS expression in the tissues surrounding hair follicles was down-regulated in both groups. Conclusions : These results suggest that Yonnyuniksoogobon-dan promotes hair growth and this effect is related to up-regulation of EGF and VEFG expression and down-regulation of TGF-${\beta}$1 and iNOS expression on hair roots.

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Distribution of Vascular Plants of Halmibong(Mt.) and Gusibong(Mt.) located in Baekdu trail of Korea (백두대간 할미봉과 구시봉 일대의 관속식물상)

  • Park, Sam-Bong;An, Jong-Bin;Park, Jeong-Geun;Kim, Jin-Jung;Ha, Hyoun-Woo;Kim, Bong-Gyu;Choo, Gab-Chul
    • Korean Journal of Environment and Ecology
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    • v.29 no.6
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    • pp.819-841
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    • 2015
  • To investigate the distribution of vascular plants growing at Halmibong(1,026m) and Gusibong(1,014m) in Baekdutrail, we surveyed from March in 2013 to March in 2014. The flora of Haimibong and Gusibong in Baekdu trail were found to be; 528 taxa; 95 families, 287 genus, 454 species, 4 subspecies, 59 varieties, 11 forma. Rare plants were found to be; 15 taxa, 10 families, 14 genus, 13 species, 2 varieties. Among them EN degree was found to be 1 species(Rhododendron tschonoskii Maxim.), VU degree; 2 species(Scorzonera albicaulis Bunge, Lilium cernuum Kom.), LC degree; 12 species(Abies koreana Wilson, Coreanomecon hylomeconoides Nakai, Viola albida Palib. etc.) respectively. In all surveyed areas, a total of 15 taxa(Heloniopsis koreana Fuse, N.S.Lee & M.N. Tamura etc.) were found to be endemic to Korea. Distribution of floristic special plants in the surveyed areas were found to be divided into five classes (class I~V). The floristic special plants found in surveyed areas were 2 taxa of grade V, 6 taxa of grade IV, 12 taxa of grade III, respectively. The naturalized plants were investigated as 23 taxa; Rumex crispus L., Amorpha fruticosa L., Veronica arvensis L. etc..

Case Study on the Countermeasure Methods and Collapsed Sources of Segmental Retaining Wall Considering Site Conditions (시공환경을 고려한 블록식 보강토옹벽의 붕괴요인 분석 및 대책방안 사례연구)

  • Han, Jung-Geun;Cho, Sam-Deok;Jeong, Sang-Seom;Lee, Kwang-Wo;Kim, Ji-Sun
    • Journal of the Korean Geosynthetics Society
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    • v.4 no.3
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    • pp.35-43
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    • 2005
  • The geosynthetic reinforced segmental retaining walls(SRW) are improved that the disadvantage of existed retaining wall and the workability in field. Recently, the segmental retaining wall is replacing the exited wall because it is quickly advanced to using by the block in-situ. The use, therefore, is increasing. But, the trends of the large scaled construction was developed that the problems likely to crack and collapse, those are caused of careless in design and construction of SRW not considering about various surrounding conditions. In this study, the cause analysis on destructed SRW was carried out that based on the datum of measured displacement of walls, rainfall features and ground sounding conditions. Also, the analysis of the global slope stability was carried out on collapsed section and non-collapsed section using critical equilibrium method. For the rational stability and analysis of slope including SRW structure, the site conditions including situations of topography, ground and histories of construction and collapse etc should be considered. The rational countermeasure methods for non-collapsed and collapsed areas may be sustained as much as possible current state.

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Fortification of γ-aminobutyric acid and bioactive compounds in whey by co-fermentation using Bacillus subtilis and Lactobacillus plantarum (유청을 이용한 Bacillus subtilis와 Lactobacillus plantarum의 혼합발효를 통한 γ-aminobutyric acid와 생리활성물질 강화)

  • Kim, Geun-young;Lim, Jong-soon;Lee, Sam-pin
    • Korean Journal of Food Science and Technology
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    • v.50 no.6
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    • pp.572-580
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    • 2018
  • Biologically active substances including gamma-aminobutryric acid (GABA) were added into whey by co fermentation using Bacillus subtilis HA and Lactobacillus plantarum EJ2014. The first fermentation using B. subtilis HA with 5% monosodium glutamate (MSG) and 2% glucose enhanced the production of poly-${\gamma}$-glutamic acid (PGA), resulting in higher consistency of $4.09Pas^n$ as well as whey protein peptides. After the second fermentation using L. plantarum EJ2014, the remaining MSG (3.40%) as a precursor was completely converted to 2.21% GABA. Furthermore, the lactose content in whey decreased from 6.73 to 3.68% after co-fermentation, and the tyrosine content increased from 20.47 to 38.24%. Peptides derived of whey proteins were confirmed by SDS-PAGE. Viable cell counts of B. subtilis and L. plantarum were 5.83 log CFU/mL and 9.20 log CFU/mL, respectively. Thus, co-fermentation of whey could produce the novel food ingredient fortified with biologically active compounds including GABA, ${\gamma}$-PGA, peptides, and probiotics.

Regulation of Anthocyanin Biosynthesis by Light and Nitrogen in Sarracenia purpurea (Sarracenia purpurea에서 빛 및 질소 의존성 anthocyanin 생합성)

  • Yoon, Joon Sup;Lee, Seung hi;Riu, Young Sun;Kong, Sam-Geun
    • Journal of Life Science
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    • v.29 no.10
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    • pp.1055-1061
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    • 2019
  • Sarracenia purpurea as a carnivorous plant in the family Sarraceniaceae is known to require strong light for its growth and to absorb nutrients from the decomposed molecules of insects that are attracted by color, sweet juice, and the like. S. purpurea grew greenish in whole body under weak light conditions, while the whole of the insectivorous sac including leaves, is changed to dark red under strong light conditions. The phenomenon of reddish S. purpurea is thought to be related to the flavonoid pigment anthocyanin. Interestingly, the color change was not observed when S. purpurea was grown in a growth condition with abundant nitrogen fertilizer. The expression levels of anthocyanin contents and biosynthesis-related genes were strongly correlated with light intensity and nitrogen fertilizer. The anthocyanin content in the strong light condition ($240{\mu}mol\;m^{-2}s^{-1}$) was 6.15 times higher than that in the weak light ($40{\mu}mol\;m^{-2}s^{-1}$). In contrast, the anthocyanin contents were not significantly changed when 0.8% urea solution was supplied as nitrogen fertilizer. Consistently, CHALCONE SYNTHASE (CHS) gene was up-regulated by strong light and down-regulated by nitrogen fertilizer. These results suggest that the environmental changes of light and nitrogen in soil regulate the anthocyanin content in S. purpurea.

Development of electrochemical biosensor for determination of galactose (4갈락토오즈 측정을 위한 전기화학적 바이오센서 개발)

  • Park, Kap Soo;Cho, Soon Sam;Quan, De;Lee, Jae Seon;Cha, Geun Sig;Nam, Hakhyun
    • Analytical Science and Technology
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    • v.20 no.5
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    • pp.393-399
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    • 2007
  • In principle, the blood galactose level may be determined conveniently with a strip-type biosensor similar to that for glucose. In this study, we describe the development of a disposable galactose biosensor strip for point-of-care testing. The sensor strip is constructed with screen-printed carbon paste electrode (SPCE) and sample amount (< $100{\mu}L$). The developed strip the galactose level in less than 90 s using bienzymatic system of galactose oxidase (GAO) and horseradish peroxidase (HRP). The effects of pH, mediator (1,1-ferrocenedimethanol) concentration, ratio of enzymes, and applied potential were determined preliminarily with glassy carbon electrodes, and optimized further with the strip-type electrodes. The sensor exhibits linear response in the range of $0{\sim}400{\mu}M$ ($r^2$ = 0.997, S/N = 3). Since a low working potential, in principle, the fabricated disposable galactose biosensor has -100 mV (vs. Ag/AgCl), it is applied for the detection of galactose, interfering responses from common interferents such as ascorbic acid, uric acid and acetaminophen could be minimized. The sensor has been used to determine the total galactose level in standard samples with satisfactory reproducibility (CV = 5 %).

Impacts of Temperature, Salinity and Irradiance on the Growth of Ten Harmful Algal Bloom-forming Microalgae Isolated in Korean Coastal Waters (한국연안에서 분리한 적조형성 미세조류 10종의 성장에 미치는 온도, 염분, 광도의 영향)

  • Lee, Chang-Kyu;Lee, Ok-Hee;Lee, Sam-Geun
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.10 no.1
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    • pp.79-91
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    • 2005
  • In order to understand growth characteristics of ten major species of microalgae responsible for frequent harmful algal blooms in Korean coastal waters, the growth rates of the isolates were examined in relation with the impacts of water temperature, salinity and irradiance. In addition, their bloom events since 1990 as well as monthly abundance of vegetative cells were analyzed. Heterocapsa triquetra, Eutreptiella gymnastica and Alexandrium tamarense were considered as relatively mid temperature adapted species in that growth rates were comparatively high at low water temperatures of $10{\sim}16^{\circ}C$ and drastically decreased at above $22^{\circ}C$. Prorocentrum micans and Pyramimonas sp. were categorized as relatively high temperature adapted species by showing comparatively better growths at high water temperatures above $25^{\circ}C$. Akashiwo sanguinea, Heterosigma akashiwo, Prorocentrum minimum and Scrippsiella trochoidea were eurythermal species with relative high growth rates in a broad ranges of water temperature, $16{\sim}25^{\circ}C$ were slightly halophobic, showing better growths at low salinities of $10{\sim}30$ psu than at above 35 psu. H. akashiwo, P. minimum and H. triquetra were euryhaline species with remarkable growths in a broad ranges of salinity, 15-40 psu. Frequent algal blooms by these three species at extremely low salinities below 25 psu after rainfall were attributed to their euryhaline and slightly halophobic physiological characteristics. Growth rates of H. akashiwo, P. minimum and Pyraminonas sp. increased with the increase of irradiance within the experimental ranges of $2{\sim}150\;{\mu}E{\cdot}m^{-2}{\cdot}s^{-1}$. However, A. sanguinea, A. tamarense and H. triquetra showed better growths at comparatively low irradiance of $50{\sim}100\;{\mu}E{\cdot}m^{-2}{\cdot}s^{-1}$ and drastic decreases in growth rates above $150\;{\mu}E{\cdot}m^{-2}{\cdot}s^{-1}$ of irradiance. Overall, relatively high temperature adapted species make blooms frequently in high water temperature season with strong natural irradiance, and relatively low temperature adapted species grow better at low water temperature with relatively weak natural irradiance.