• Title/Summary/Keyword: Lee Don-Hwa

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옥수수중 Deoxynivalenol의 검출을 위한 효소면역측정법의 개발

  • Lee, Hyang-Burm;Shon, Dong-Hwa;Kosaka, Kunio;Ueno, Yoshio
    • Microbiology and Biotechnology Letters
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    • v.25 no.4
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    • pp.414-419
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    • 1997

  • In order to develop an enzyme-linked immunosorbent assay (ELISA) for deoxynivalenol (DON) in com, we produced a specific monocl- onal antibody and established ELISA conditions. After the spleen cells from mice immunized with DON-bovine serum albumin conjugate were fused with S$_{p}$2/0 myeloma cells, a hybridoma cell 3G7 producing anti-DON antibody was screened by ELISA. From the standard curve of competitive direct ELISA (cdELISA) using 3G7 monoclonal antibody and DON-HRP conjugate, the detection range of DON showed 3-3,000 ng/ml (ppb). The monoclonal antibody showed some cross-reactivities against DON analogues such as 15 acetyl-DON (110%), nivalenol (5.0%), 3 acetyl-DON (1.7%), fusarenon-x (0.72%), and T-2 (0.59%). When the cdELISA was applied to the spiked coms after extracting with 60% methanol and diluting 5- fold with washing buffer, the assay recoveries of DON were 313, 163, 106, and 88.9% (av., 168%) in the levels of 200, 600, 2,000, and 6,000ng/g, respectively. For the quantitation of DON in coms, 30 samples kept under two different storage conditions of cold and room temperature were assayed by cdELISA. The mean detection concentrations were 595 (detection range, 0-2,750) and 2,448 (detection range, 0-4,500) ppb, respectively.

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Effects of Barely Processings on the Reduction of Deoxynivalenol (보리 가공에 의한 Deoxynivalenol의 감소 효과)

  • Pei, Shi-Chun;Lee, Won-Jong;Ryu, Gi-Hyung;Chung, Duck-Hwa
    • Journal of Food Hygiene and Safety
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    • v.19 no.3
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    • pp.119-125
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    • 2004

  • This study examined the effects of pearling, simple heating, baking and extrusion in processing of barley contaminated with deoxynivalenol (DON) on the reduction or destruction of DON. The DON contamination level of barley used in the experiment was 2.08 ppm. The DON-forming strain isolated from the barley was Fusarium graminearum, and the DON formation potential of which was 6.3 ppm on the average in Czapek-Dox broth medium. The DON removal efficiency of pearling was 56.5%, simple heating 3.8-32.7%, extrusion 53-59%, and baking 10.4%. As a large quantity of DON still remains after processing, it tis necessary to develop a new method of removing DON comletely.

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A Study on the Comparison of Liang Shu-Ming and Lee Don-Hwa's Perceptions of Modernization and the Philosophical Grounds of their Perceptions : Focusing on Works during the New Cultural Movement Period in Korea and China (양수명과 이돈화의 근대화에 대한 견해 및그 철학적 근거에 대한 비교 연구 - 한·중 신문화운동 시기의 저작을 중심으로 -)

  • Hwang, Jong Won
    • The Journal of Korean Philosophical History
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    • no.36
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    • pp.319-352
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    • 2013

  • This paper aims to compare Liang Shu-Ming and Lee Don-Hwa's perceptions of modernization and the philosophical grounds of their perceptions during the New Cultural Movement period in Korea and China. Although Liang and Lee had different levels of enthusiasm towards New Cultural Movement, they shared many commonalities in their attitudes to Western modernization and their philosophical justifications for such attitudes. Liang Shu-Ming not only held positive views of Western modernization, but also examined the spiritual dynamics contributing to the development in science and democracy in Western countries. By analyzing the three fundamental questions of life, three kinds of human attitudes toward life, three types of culture derived from human attitudes, and the recapitulation theory, Liang systematically reviewed the spiritual dimension of Western modernization. Liang defined the spirit of Western modernization as a calculative rationality based on egoism and the separation of Self and Other. Such a statement demonstrates Liang's keen insights towards Western modernization. Meanwhile, although Lee Don-Hwa basically held positive views of Western modernization, from the beginning, he pointed out the problems with Western modernization and attempted to overcome these problems by employing the "human is heaven" doctrine of Cheondogyo. While Lee embraced modern science and the theory of evolution, he also insisted on the legitimacy of mysticism and the compatibility of the creation theory of Cheondogyo and the theory of evolution. Although Lee employed the concepts in Western philosophy of life, he also emphasized the interdependence, reciprocal relationship, and connectedness between Self and Other from the perspective of Cheodogyo doctrine. From the fact that Lee Don-Hwa transcended the modernization theory when he was applying it to answer questions, it seemed that Lee preceded Liang Shu-Ming. Nevertheless, Lee did not demonstrate the keen insight into the spiritual dimension of Western modernization, and his criticism against Western modernization did not touch upon the problem of rationality as Liang did.

A Case of Pemphigus vulgaris with Generalized Pruritus (전신소양감을 동반한 심상성 천포창 치험 1례)

  • Lee, Sung-Eun;Yoon, Hwa-Jung;Ko, Woo-Shin
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.29 no.2
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    • pp.112-122
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    • 2016

  • Objectives : The purpose of this report is to know the effect of Korean medical treatments on pemphigus vulgaris with generalized pruritus. Methods : We treated a 44-years-old woman patient with pemphigus vulgaris on the whole body with Korean medicine. After Korean medical treatment for 17 weeks, we measured the extinction of blisters and decrease of pruritus. We recorded pictures of changes on symptoms. Results & Conclusions : The symptoms of pemphigus vulgaris were significantly improved. The blisters and itching were decreased and skin damages were almost recovered. Thus Korean medical treatments are effective on pemphigus vulgaris.

  • https://doi.org/10.6114/jkood.2016.29.2.112 인용 PDF KSCI KPUBS HTML