• Applied Microscopy
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• v.28 no.3
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• pp.399-414
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• 1998

Glandular trichomes present on the leaf surface of Drosera capensis were examined using transmission electron microscopy. A large number of stalked glands exist on the adaxial surfaces of the leaf blade. The secretory head is composed of two layers of secretory cells, one layer of middle cells, and the inner tracheids. The secretory cells contain rough endoplasmic reticulum, mitochondria, plastids, Golgi apparatus, and vacuoles. The secretory cells show prominent cell wall ingrowth, and thick cuticle restricted on the subcuticular wall. Frequently, the cuticle has some pores, canal-like structures, showing electron -dense granules being penetrated through them. Ultrastructural localization using diaminobenzidine showed the electron-dense deposits in the vacuole. No peroxidase activity was seen in the cell wall and cytolasm. The activity of peroxidase (POX) isozymes in Drosera which isoelectric point (pI) is 3.6 and some anionic POX isozymes which pIs are laid between 3.6 and 4.6 were especially increased according to the development and the formation of glandular trichomes. Also, the activity of some POX isozymes which isoelectric points are laid between 4.6 and 5.1 were increased in the regions of leaves which has trichomes.

• Korean Journal of Environment and Ecology
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• v.29 no.2
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• pp.145-161
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• 2015

This research was conducted in order to understand the hybridization between Quercus aliena Blume and Q. serrata Murray in Korea which show wide range of morphological variations within species and interspecific variations of diverse overlapping characteristics caused by hybridization. Morphological analysis (principal components analysis; PCA) of 116 individuals representing two species and their intermediates were performed. As a result, two species were clearly distinguished in terms of morphology, and intermediate morpho-types assumed to be hybrids between the two species were mostly located in the middle of each parent species in the plot of the principal components analysis. There was a clear distinction between two species in trichome distribution pattern which is an important diagnostic character in taxonomy of genus Quercus, whereas intermediate morpho-types showed intermediate state between two species' trichome distributions. Forty-two individuals representing two species and their intermediates were examined for leaf flavonoid constituents. Twenty-three flavonoid compounds were isolated and identified: They were glycosylated derivatives of flavonols, kaempferol, quercetin, isorhamnetin and myricetin. The flavonoid constituents of Q. aliena were five glycosylated derivatives: kaempferol 3-O-galactoside, kaempferol 3-O-glucoside, quercetin 3-O-galactoside, quercetin 3-O-glucoside, and Isorhamnetin 3-O-glucoside. The flavonoid constituents of Q. serrata had 20 diverse flavonol compounds including five flavonoid compounds found in Q. aliena. It was found that there is a clear difference in flavonoid constituents of Q. aliena and Q. serrata. Flavonoid chemistry is very useful in recognizing each species and putative hybrids. The flavonoid constituents of intermediates were a mixture of the two species' constituents and they generally showed similar characteristics to morpho-types. The hybrids between Q. aliena and Q. serrata showed morphologically and chemically diverse characteristics and it is assumed that there are frequent interspecific hybridization and introgression.

• Asian Journal of Turfgrass Science
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• v.26 no.1
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• pp.1-7
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• 2012

Field experiments were conducted to evaluate the morphological characteristics and growth rates of 101 medium-leaf type zoysiagrasses (Zoysia spp.) collected at the major sod production area (Jang Seong Gun) in South Korea. Collected lines with distinctive morphology and visual growth rate were planted in plastic pots and measured morphological characteristics under the plastic house conditions. Variation of leaf width, plant height, leaf angle, length of leaf sheath, trichome, stolon length, and color were measured. Six lines were selected by evaluating growth rates from one hundred one collected lines. Eight standard cultivars and three other superior lines previously collected were compared to 7 selected lines form Jang seong area by checking growth rates and morphological characteristics. Average leaf blade width was 3.4 mm, leaf angle was 45.8 degree, plant height was 21.6 cm, height of lowest leaf was 5.0 cm, and length of leaf blade was 14.1 cm. Ground cover rates of selected lines 'CY6097' and 'CY6069' were 70% and 68.3%, respectively. These are believed to be faster than 60% ground cover rate of zoysiagrass 'Anyang', and also, twice as faster than the 31.7% ground cover rate of Z. matrella. Selected line 'CY6069' showed fast growth rate with shorter internode length (5.1 cm) compared to zoysiagrass 'Anyang'. Based on the results of this study, we could select useful fast growing zoysiagrass breeding lines from the major sod production area (Jang Seong Gun) in Korea.

• Journal of Korean Society of Forest Science
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• v.64 no.1
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• pp.52-63
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• 1984

This study was intended to identify 17 taxa. (5 varieties and 12 species) of the genus Acer in Korea on the basis of the shapes of stomata, the type of trichome on the different part of leaves, the shapes, arrangement and number of stele in cross section of petiole. The results obtained were summarized as follows; 11 The shape of guard cells of stomata in the genus Acer was anomcytical, and the size of the cells ranged from 10.25 to $21.00{\mu}$ in length and from 7.57 to $11.83{\mu}$ in width. 2) Eleven types of trichome on the leaf in the genus Acer were found; pilose, sericeous, velutinous, woolly, glabrate, puberulent, bladder hair, hispid, hirsute and uncinate. This characteristics also established a good criterion for identification of species. 3) The stele of petiole in the genus Acer was characterized by eustele and atactostele with polybranch, and the six groups of the shape of numerical change of stele; B>M=T, BM

• Korean Journal of Plant Resources
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• v.12 no.1
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• pp.50-61
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• 1999

The leaf morphological and anatomical characters about 17 taxa of Aster and its allied taxa were investigated to estimate taxonomic values. Leaf shapes of the treated taxa were divided into five types; elliptic, spathulate, lanceolate, linear, ovatodeltoid. These types were fixed in same taxa, but variable among different taxa, therefore useful as taxonomic character. Leaf margins were divided into four types; entire, serrate, dentate, incised, these types were invariable in most taxa, but variable among individual in same taxa such as Kalimeris incise, Aster spathulifolius. The size and shape of leaf epidermal cell, the size and distributional numbers per 10$\textrm{mm}^2$ of stomata, deposit feature of cuticle were not distinguished clearly from treated taxa, but presence of stomata on adaxial surface, size and distributional numbers per 10$\textrm{mm}^2$ of stomata were useful taxonomic characters in some taxa such as Gymnaster koraiensis, Aster altaicus var. uchiyamae, Aster tripolium, Heteropappus arenarius. The leaf trichomes were divided into five types by basic form, sculpturing on their outer surface and cell arrangement; uniseriate granulate conical type, uniseriate psilate conical type, uniseriate psilate filiform type, globular type, biseriate vesicular capitate type. In spite of various habitat, basic morphology of trichomes were not changed, therefore, it was thought to be good taxonomic character.

• Journal of Korean Society of Forest Science
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• v.88 no.1
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• pp.11-17
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• 1999

Morphological variations in leaves and leaf trichomes according to tree age were investigated for the identification of Quercus taxa at the different stages of development. The sizes of leaves from one-year-old seedlings of Q. serrata, Q. dentata, and Q. aliena were the smallest and increased with tree age. Blade length, blade width, petiole length, number of serration, and serration depth war also varied with tree age. Therefore, the examined taxa by the size and form of leaves were hardly identified at juvenile stages. The type, structure, and density of leaf trichomes of each species varied remarkably along with tree age. Three types of trichomes such as small stellate hairs, large stellate hairs, and long single hairs were observed on the abaxial side of leaves of Quercus taxa. In matured trees of Q. serrata both small stellate and long single hairs on leaves were observed. On the other hand large stellate hairs were found in leaves of Q. dentata. There were small stellate hairs in Q. aliens. In Q. mongolica var. crispula, no hair or small stellate hairs were occasionally found. It is possible to identify Q. serrata and Q. dentata by the characteristics of trichome even at two to three year old seedlings, while, it is difficult to tell Q. aliens and Q. mongolica var. crispula of seedlings from other taxa only by those of trichome.

• Korean Journal Plant Pathology
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• v.3 no.2
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• pp.100-107
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• 1987

Pre-pentration activity of Pyricularia oryzae Cav. from the stage of conidia germination to appressorium formation was examined on rice leaf epidermis under light and scanning electron microscopes to determine the causes· for differences in blast susceptibility between plants grown under three different soil moisture conditions in the greenhouse. No significant differences were found in the external shape of leaf epidermal cells including bulliform cells between plants grown under different soil moisture conditions. Growth and orientation of germ tube and morphology and size of appressorium of P. oryzae did not vary with soil moisture treatment. Site of appressorium formation was consistent over soil moisture treatment with the highest frequency of bulliform cell $(35\~48\%)$, followed by short cell $(19\~27\%)$, and long and guard cells $(13\~20\%)$. No appressorium was formed on trichome. This result suggests that the observed differences in blast susceptibility between plants grown under different soil moisture conditions were not due to the differences in the pre-pentration activity of P. oryzae on those plants.

• Korean Journal of Plant Tissue Culture
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• v.27 no.2
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• pp.117-123
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• 2000

In order to study the role of polyamines on the formation of adventitious roots, trichomes and calli, the effects of putrescine, spermidine, spermine, cyclohexylamine (CHA) and methylglyoxal-bis(guanylhydrazone) (MGBG) were investigated in the leaf segment cultures from ecotype Columbia of Arabidopsis thaliana. When the leaf segments were cultured on the media for forming adventitious roots (0.1 mg/L NAA), trichomes (2.0 mg/L NAA) and calli (10.0 mg/L NAA), and then each cultures was treated with 1-100 mg/L of putrescine, spermidine and spermine, respectively. On the adventitious root-forming medium treated with polyamines the trichomes were induced with adventitious roots. And on the trichome-forming medium with polyamines calli were induced with trichomes. In orther hand each cultures was treated with 1-100 mg/L of CHA and MGBG, respectively. CHA promoted adventitious roots on the medium for adventitious roots, was not effected on media for trichomes and calli. MGBG inhibited adventitious roots, trichomes and calli in all cultures, and induced adventitious roots on medium for trichomes in high concentration.

• Korean Journal of Plant Resources
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• v.29 no.5
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• pp.625-629
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• 2016

A new Gomchwi cultivar ‘Gommany’ was bred by crossing between Gomchwi (Ligularia fischeri (Ledeb.) Turcz.) and Handaeri-gomchwi (Ligularia fischeri var. spiciformis Nakai). The selection and investigation of growth and yield characteristics were conducted from 2006 to 2011 in field and greenhouse of Highland Agriculture Research Institute, NICS, Rural Development Administration. The color of petiole ear was purple. Petiole trichome existed, but light of leaf back did not. Density of leaf vein was 5 degree in a newly developed cultivar ‘Gommany’. Plant height, leaf length, leaf width and petiole length were 47.7, 16.3, 21.2, and 31.4 ㎝, respectively in the 3 year of growth characteristics. Plant size was also higher than that of ‘Gondalbi’. Bolting and flowering time were Aug. 5 and Sept. 10, respectively. In contrast, Bolting time of ‘Gondalbi’ showed 25 days quite earlier bolting time compared to ‘Gommany’, Which led to earlier flowering time. ‘Gommany’ showed higher number of leaves (138) per plant compared to ‘Gondalbi’ (130). Furthermore, yield was 97% higher in ‘Gommany’ (1,575 g/plant) than in ‘Gondalbi’ (798 g/plant). ‘Gommany’ showed higher leaf thickness (0.69㎜) than ‘Gonalbi’ (0.46 ㎜), and consequently showed more hardness in leaf characteristics (24.3 ㎏/㎠) compared to ‘Gondalbi’ (23.0㎏/㎠). ‘Gommany’ showed higher resistance in the susceptibility of powdery mildew compared to ‘Gondalbi’. ‘Gommany’ cultivar was registered as a novel variety with number 90 in The Minister of the Korea Forest Service on December 2015.

• Korean Journal of Plant Resources
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• v.34 no.4
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• pp.339-345
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• 2021

'Gondalbi' (Ligularia stenocephala (Maxim.) Matsum. & Koidz.) is the most cultivated Gomchwi species because of higher yield and low in aromatic flavor and bitter taste, But 'Gondalbi' is susceptible to powdery mildew disease and leaf shriveling after harvest in Summer. To improve powdery mildew disease resistance and post-harvest leaf shriveling problem in 'Gondalbi', 'Handeari-gomchwi' resistant to powdery mildew disease and having higher yield potential used as a paternal trait donor. Powdery mildew disease resistance and post-harvest leaf shriveling as well as agronomic performance of a new variety, 'Greenbear' were tested under field and green house conditions in Pyungchang, Korea from 2007 to 2016. Expression of both maternal and paternal characteristics in 'Greenbear' including purple colored petiole ears, glossy leaf and paternal, petiole trichome, absent at the back of a leaf were confirmed. Plant size and flowering time of 'Greenbear' were similar to check line, 'Gommany', while leaf number per plant and yield were higher in 'Greenbear'. 'Greenbear' has thinner leaves (0.66mm) compared to 'Gommany (0.69 mm)', and hardness appeared slightly higher in 'Greenbear(25.1 kg/cm²)'. The resistance to powdery mildew disease of the 'Greenbear' variety was slightly lower than that of the 'Gommany' variety, but somewhat showed high resistance.