• Journal of the Korean Applied Science and Technology
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• v.35 no.1
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• pp.254-262
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• 2018

It was intended to check a possibility of activity of Psidium guajava leaf extract as anti-oxidant and anti-inflammatory material. Total polyphenol and total flavonoid content of Psidium guajava leaf extract was checked. Cream having inhibitory effect on inflammation through toxicity and NO production inhibition in RAW 264.7 cell was manufactured, and skin safety was evaluated. It was confirmen that total polyphenol and flavonoid content of Psidium guajava leaf extract was 126.4 mg/g and 223.17 mg/g respectively, which was high content. According to the results of checking toxicity through cell viability in RAW 264.7 cell, cytotoxicity was not shown. And NO production indicating inflammatory disease was inhibited concentration-dependently. According to the results of carrying out single patch test after manufacturing the cream containing the Psidium guajava leaf extract, skin irritation did not occur for 24 h to put patch on skin or for 24 h after removing the patch. Putting these results together, it was verified that there was possibility of application as raw materials for cosmetics, which would have anti-oxidant activity owing to the high polyphenol and flavonoid content of Psidium guajava leaf extract and anti-inflammatory material through NO production inhibition.

• Applied Chemistry for Engineering
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• v.24 no.3
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• pp.260-265
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• 2013

In this study, nano-emulsions containing 0.01, 0.03, 0.05, and 0.10% ethyl acetate fraction of Hippophae rhamnoides (H. rhamnoides) leaf extracts were prepared. The particle size, particle size distribution and skin permeability of the nano-emulsions were evaluated for five weeks. Nano-emulsion was prepared by the sequential use of homogenizer and microfluidizer. Nano-emulsion containing the ethyl acetate fraction exhibited a monodispersed form. Nano-emulsion containing 0.03% ethyl acetate fraction was the most stable for five weeks. The in vitro skin permeation study of nano-emulsion containing 0.03% ethyl acetate fraction was carried out using Franz diffusion cell. The nano-emulsion showed a better skin permeability than that of O/W emulsion. These results indicate that the nano-emulsion containing the ethyl acetate fraction of H. rhamnoides leaf extract showed a remarkable stability and skin permeability than that of O/W emulsion.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.16 no.3
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• pp.164-184
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• 2003

Objectives : This study was designed to establish a characteristic discrimination of internal and external morphological standard of original plants and herbal states in Pogostemonis and Agastachis Herba. Methods : In this studies, the external-internal morphological standards were determined by using stereoscope and butanol series. Results : 1. The external characteristics: Pogostemon cablin has hairs and brown-like in stem, elliptical fruit. On the other hand, Agastache rugosa has no hairs and red-like in stem, obovatic trigone fruit. 2. The physical characteristics: Pogostemon cablin is gray in whole, has hairs in stem and numerous hairs of ash in leaf. On the other hand, Agastache rugosa is yellow-green in whole, has no hairs in stem. Specially the latter has deep-green colour and numerous hairs presenting mostly at lower epidermis in leaf. 3. The physical characteristics in currents: Pogostemon cablin is brown, has hairs and round-like stem. On the other hand, Agastache rugosa is green or yellow-green, has no hairs and tetragon in stem. 4. The internal characteristics: Pogostemon cablin has progressed spongy tissue in epidermal cell of leaf and many rank of epidermal cell in stem. On other hand, Agastache rugosa has I rank palisade tissue in leaf and few rank of epidermal cell in stem. In the external shape, it was possible that herbs were distinguished according to artificial cIassification and that same genus-degree of relatedness among herbs could be distinguished by more precise and active observation. In the shape of real herbs, I compared current herbs in market with original herbs which were just collected or were on the course of drying. In addition, it was possible that the internal shape could be identified by using microscope after butanol series. Conclusion : Though it was impossible to make distinction of herbs which are not current in my search contents, this search contents will be a standard for applying herbs in the future. An Additional standard establishment including physiochemical reaction and gene research is required in order to supplement the fault of this search.

• Journal of Bio-Environment Control
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• v.16 no.1
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• pp.62-66
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• 2007

This study was carried out to develop the stable plug seedling production for hydroponics of spinaches by various nursery media, plug tray size and seed number per plug tray cell. When plant grown in various nursery media, the seeding stand rate was shown in order of granular rockwool with good water retention, granular rockwool>granular rockwool mixed with pearlite>cocopeat>pearlite>poly urethane foame. Thus, poly urethane foame indicated the lower seedling stand rate. There was no difference in growth of the seedlings md the seeding stand rate by the plug tray size, and no significant difference in the plant height and the number of leaves among the seed number per plug tray cell. But, leaf area of plant in 2 grains seeding per cell was $113.0cm^2$, was wider in compared with 5 grains seeding of which leaf area was $88.0cm^2$. Accordingly, the leaf area per plant decreased as more and more the number of seeds per plug tray cell increased. The fresh weight of a plant per plug tray cell was the heaviest with 12.5g in the 2 grains, and the total fresh weight of plants per cell was 33.9g in 4 grains seeding, thus it tended to was bigger compared with other treatments. Consequently, given that the number of seeds per cell was decreased, the fresh weight of a plant increased. On the other hand, the total fresh weight per cell showed a tendency to be reducing as more and more the number of seeds per plug tray cell decreased. The yield in the 4 grains seeding was increased by 46% as $14,910kg{\cdot}ha^{-1}$ in compared with the yield in 2 grains seeding as $10,200kg{\cdot}ha^{-1}$.

• Journal of Ginseng Research
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• v.7 no.2
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• pp.169-171
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• 1983

The structural components of cell wall in various parts of Korean ginseng were analysed. Pectin was abundant in the cortex and epidermis of root, and leaf. Hemicellulose in root was more than in other parts of ginseng. Cellulose content of stem and seed coat was much higher compared to other parts and lignin content was highest in seed coat.

• Preventive Nutrition and Food Science
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• v.10 no.2
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• pp.172-178
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• 2005

Dandelions have been reported to have medicinal properties and bioactive components that impact human health. However, the precise biological properties of dandelions and the parts of the plants possessing bioactive components remain uncertain. In this study, we evaluated 3 different types of dandelions based on their cultivation origin (Songpa, Uiryung, and native Uiryung types) as well as their 4 different plant parts (leaf, flower, root, skin). Each sample was extracted with $80\%$ methanol and then compared for the biological activities (anti-oxidative, immune cell proliferative and tumor cell growth inhibitory activities). All 3 types of dandelions possessed a degree of biological functions including the hydroxyl radical scavenger activity, immune cell proliferative activity and tumor cell growth inhibitory activity. However, there was no significant difference in these activities between the 3 dandelion types. Leaves of all three dandelion types showed the highest levels of all biological activities. To a lesser degree, the flower and root parts displayed biological activities. In the skin parts, anti-oxidative activity was also detected only at higher doses of dandelion extracts. Heating the dandelion leaf extract did not affect the biological activity, suggesting a heat-stable nature of the biological compounds. Taken together, these collective data suggest that dandelions, in particular their leaves, possess a high concentration of heat-resistant biological compounds, which are responsible for anti-oxidative, immune cell proliferative and tumor cell growth-inhibitory activities.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.36 no.1
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• pp.70-73
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• 1991

Stomatal frequency and size of rice plant grown in the different silicon levels of the culture solution in phytotron were observed on both side of the 7th leaf blade. By the application of silicate, stomatal frequency and rates of stoma cell area to the leaf area were remarkably reduced in Milyang 23. It could be considered as favorable characteristics which might be reduced the transpira-tion rate from leaf surface of rice plant besides presence of cuticle-silica double layer in leaf surface.

• Journal of Plant Biology
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• v.39 no.4
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• pp.237-242
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• 1996

Epidermal leaf cell phytoliths of 17 species of Oryza were examined with backscattered electron imaging with scanning electron microscopy to determine the usefulness of phytolith morphology for systematic studies. Many kinds of identifiable phytolith morphology are observed in the leaf blades of Oryza. They are different among species as well as between adaxial and abaxial surface and costal and intercostal strip portions of leaf blades. Distinguishing phytolith characters are useful at the level of the section, such as Angustifoliae, Ridleyanae, and Granulatae. The results of a cluster analysis using 21 qualitative characters of phytoliths reveal many taxonomic characteristics which are compatible with current taxonomy.

• The Plant Pathology Journal
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• v.17 no.5
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• pp.249-256
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• 2001

Xanthomonas axonopodis pv. glycines 8ra produces a bacteriocin called glycinecinA, which specifically inhibits the growth of bacteria belonging to Xanthomonas species. GlycinecinA was produced by culturing Escherichia coli DH5 containing biosynthetic genes for glycinecinA, and was tested for its control effect against X. vesicatoria on red pepper and X. oryzae pv. oryzae on rice. The bacteriocin activity was much higher in the cell extract than in the supernatant. It reached a maximum level at the stationary phase, ws maintained up to 2 months at room temperature and approximately 10 months at $4^{\circ}$. The optimum concentration of glycinecinA for the control in the greenhouse and in the field was 12,800 AU/ml. In this study, the activity of glycinecinA on rice and red pepper leaves continued for 7-8 days, during which the pathogen populations remained at low levels. Bacterial leaf spot of red pepper and bacterial leaf blight of rice were significantly reduced by the bacteriocin treatments. The control efficacy was as high as, or even higher than, the chemical treatment of copper hydroxide. These results suggest that the bacteriocin is a potential control agent for bacterial diseases.

• Journal of Photoscience
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• v.4 no.1
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• pp.23-30
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• 1997

Leaf movements in nyctinastic plants are produced by changes in the turgor of extensor and flexor cells, collectively called motor cells, in opposing regions of the leaf movement organ, the pulvinus. In Samanea saman, a tropical tree of the legume family, extensor cells shrink and flexor cells swell to bend the pulvinus and fold the leaf at night, whereas extensor cells swell and flexor cells shrink to straighten the pulvinus and extend the leaf in the daytime. These changes are caused by ion fluxes primarily of potassium and chloride, across the plasma membrane of the motor cells. These ion fluxes are regulated by exogenous light signals and an endogenous biolgical clock. Inward-directed K$^+$ channels are closed in extensor and open in flexor cells in the dark period, while these channels are open in extensor and closed in flexor cells in the light period. Blue light opens the closed K$^+$ channels in extensor and closes the open them in flexor cells during darkness. Illumination of red light followed by darkness induces to open the closed K$^+$ channels in flexor and to close the open K$^+$ channels in extensor cells in the light. The dynamics of K$^+$ channels in motor cells that are controlled by light signals are consistent with the behavior of the pulvini in intact plants. Therefore, these cell types are an attractive model system to elucidate regulations of ion transports and their signal transduction pathways in plants. This review is focused on light-controlled ion movements and regulatory mechanisms involved in phosphoinositide signaling in leaf movements in nyctinastic plants.