• Tuberculosis and Respiratory Diseases
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• 제44권3호
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• pp.601-610
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• 1997

연구배경 : 그람음성 간균의 내독소에 의한 sepsis syndrome에서 단핵 식세포는 내독소에 의해 자극받아 여러 종류의 cytokine을 분비함으로써 개체를 방어하는데 있어 매우 중요한 역할을 한다. 그러나 불행히도 cytokine들은 개체를 방어하는 작용 뿐 아니라 TNF등의 경우처럼 심각한 조직손상을 가져오는 효과도 있다. 그러므로 생체 내에서 내독소에 반응하여 cytokine의 분비를 조절하는 기능은 매우 중요하다. 이전의 연구에서 미리 내독소에 노출된 단색 식세포는 내독소로 재자극 시 cytokine 생성능의 저하가 관찰되었는데 이러한 현상을 '내독소 내성'이라고 하며 cytokine 분비조절에 중요한 역할을 하리라 생각되나 그 기전 등에 대해서는 연구가 부족한 상태이다. 방 법 : 생체 외에서 내독소에 대한 내성획득의 조건을 확립하고자 정상인의 말초혈액 단핵구를 10ng/ml의 저농도 내독소로 24시간 전처치한 후 2회 세척하고 다시 1 ng/ml의 내독소로 각각 4시간, 6시간, 24시간 자극하여 TNF-$\alpha$와 IL-8의 단백량을 측정하고 총 RNA를 분비하였다. 내독소 내성 획득 기전을 밝히고자 내독소 전처치 시에 자가혈청, PMA, antiCD14 Ab, Indomethacin, $PGF_2$를 각각 첨가하여 내성획득에 영향을 주는지 알아보았다. TNF-$\alpha$와 IL-8의 단백량은 ELISA를 이용하여 측정하였고 분리한 RNA를 이용하여 TNF-$\alpha$와 IL-8에 대한 Northern blot analysis를 시행하였다. 결 과 : 말초혈액을 저농도 내독소로 전처치하면 TNF-$\alpha$ 단백 생성 및 mRNA 발현을 억제하였으나 IL-8에 대해서는 이러한 현상을 관찰할 수 없었다. 전처치 시에 antiCD14 Ab를 내독소와 같이 준 경우 억제된 TNF-$\alpha$ 생성이 부분적으로 회복되었다. PMA만으로 전처치 하여도 저농도 내독소 전처치와 유사하게 내독소 내성을 유도할 수 있었다. 결 론 : 내독소에 의한 내성획득에는 CD14가 관여하고 Protein kinase C 경로를 통하며 pretranslational 수준에서 조절되는 것으로 생각된다.

• Journal of Nutrition and Health
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• 제45권5호
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• pp.479-488
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• 2012

야간에 고열량 식품 섭취는 그대로 체지방으로 축적되어 비만을 유발한다고 한다. 본 연구는 2005년 국민건강영양조사 자료를 이용하여 20세 이상의 성인 3,903명 (남자 41.6%, 여자 58.4%)을 대상으로 야식의 에너지 수준을 야식 비섭취군, 500 kcal 미만 야식군, 500 kcal 이상 야식군의 3군으로 분류하여 체위, 혈압 및 혈청성분, 식습관, 식품 및 영양소의 섭취상태를 비교하였다. 1) 전체대상자 중 야식을 섭취하는 사람은 34.0%이었고, 500 kcal 미만 야식군은 28.4%로 500 kcal 이상 야식군의 5.6%에 비해 약 5배 정도 많았다. 500 kcal 미만 야식군은 여자가 남자보다 많은 반면, 500 kcal 이상 야식군은 남자가 여자 보다 약 3.9배 정도 많았고 젊은 연령대와 대졸 이상의 학력자 및 소득 수준이 높은 사람이 많았으며, 자가진단한 건강상태가 좋지 않다고 답한 사람이 적었다. 2) 평균 체질량지수는 군 간에 차이가 없었으나, 평균 허리둘레는 500 kcal 이상 야식군에서 다른 두 군보다 높게 나타났고, 성과 연령을 보정한 후에도 허리둘레와 야식의 에너지 섭취량 간에 약한 양의 상관성을 나타내었다. 3) 이완기 혈압, 공복 혈당, 혈청 중성지방, 혈청 GPT, 혈청 크레아티닌의 농도는 500 kcal 이상 야식군에서 다른 두 군보다 높게 나타났으며, 야식의 에너지 섭취량과의 상관분석 결과 공복혈당과 경구당부하 2시간 후 혈당만이 야식 에너지 섭취량과 약한 양의 상관관계를 나타내었다. 4) 500 kcal 이상 야식군에서 아침 결식자가 많았고, 외식 횟수가 하루 3회 이상인 사람이 다른 두 군에 비해 약 2배 정도 높게 나타난 반면, 야식 비섭취군에서 간식을 안하는 비율이 높았다. 5) 500 kcal 이상 야식군에서 육 난류, 음료류, 주류, 패스트푸드류의 섭취 빈도가 다른 두 군에 비해 높게 나타났고, 영양소 섭취량을 섭취 에너지 1,000 kcal당으로 환산한 결과는 지방과 알코올의 섭취량이 높게 나타난 반면 탄수화물, 조섬유, 칼슘, 인, 철, 나트륨의 섭취량이 낮았다. 식품군 섭취량과의 상관분석 결과에서 육 난류, 음료류, 주류, 과일류, 우유 및 유제품류가 야식 에너지 섭취량과 양의 상관관계를 나타내었고, 영양소 섭취량에서 칼슘, 인, 철, 칼륨, 조섬유와 약한 음의 상관관계를 나타내었다. 6) 500 kcal 이상 야식군은 다른 두 군에 비해 탄수화물 섭취 비율이 낮은 반면 지방의 섭취 비율은 높게 나타났다. 이상의 연구결과로 부터 야식의 에너지 수준이 500 kcal 이상인 경우 주로 동물성 식품, 고지방 식품, 주류 및 음료류 등 고열량 식품군의 섭취가 높고, 허리둘레, 공복 혈당, 경구 당부하 2시간 후 혈당이 높아 야식의 빈번한 섭취가 한국 성인, 특히 젊은 층의 남성에서 복부비만과 당뇨병을 야기할 가능성을 제시하였다.

• Archives of Pharmacal Research
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• 제26권3호
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• pp.224-231
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• 2003

The anti-thrombotic effects of LB30057, a direct thrombin inhibitor, were evaluated with in vivo rat and dog thrombosis models. In rats, 1 mg/kg of LB30057 inhibited half of the clot formations in the inferior vena cava at 5 minutes after intravenous application. When measured at 2 hours after oral application, 100 mg/kg prevented approximately half of the clot formations in the inferior vena cava and 50 mg/kg prolonged the mean occlusion time from $15.6{\pm}1.3$ minutes to $47.2{\pm}8.3$ minutes in the carotid artery. In dogs, the formation of thrombus in the jugular vein was reduced to half at a dose range of 20-30 mg/kg at 6 hours after oral application. In addition, the LB30057 dosage required to reduce venous clot formation by approximately 80-90% in dogs was only about 10% of that required for the same reduction in rats. This is probably due to the variation in its time-dependent blood concentration profiles in each species; for example, the plasma half-life of LB71350 in dogs was longer than that in rats ($153.0{\pm}3.0$ vs. $129.7{\pm}12.7$ min at 30 mg/kg, i.v., respectively). AUG, $T_{max},{\;}G_{max}$, and BA in dogs were 59, 8.9, 9.17, and 13.3 times higher than those in rats at oral 30 mg/kg, respectively. Taken together, these results suggest that LB30057 administered orally is effective in the prevention of arterial and venous thrombosis in rats and dogs. It therefore represents a good lead compound for investigations to discover a new, orally available, therapeutic agent for treating thrombotic diseases.

• Asian Pacific Journal of Cancer Prevention
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• 제15권6호
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• pp.2613-2618
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• 2014

Aims: Dysfunction of the host immune system in cancer patients can be due to a number of factors, including lymphocyte apoptosis. Several studies showed that $Foxp3^+T$ cells take part in inducing this process by expressing FasL in tumor patients. However, the relationship between apoptosis, $CD8^+T$ cells and $Foxp3^+T$ cells in HCC patients is still unclear. The present study was designed to investigate the correlation between apoptosis levels and Fas/FasL expression in $CD8^+T$ lymphocytes and $Foxp3^+T$ cells in patients with HCC. Methods: $CD8^+T$ cells and $CD3^+Foxp3^+T$ cells were tested from peripheral blood of HCC patients and normal controls and subjected to multicolor flow cytometry. The expression of an apoptosis marker (annexin V) and the death receptor Fas in $CD8^+T$ cells and FasL in $CD3^+Foxp3^+T$ cells were evaluated. Serum TGF-${\beta}1$ levels in patients with HCC were measured by enzyme-linked immunosorbent assay. The relationship between apoptosis and Fas expression, as well as FasL expression in $CD3^+Foxp3^+T$ cells was then evaluated. Results: The frequency of $CD8^+T$ cells binding annexin V and Fas expression in $CD8^+T$ cells, were all higher in HCC patients than normal controls and the proportion of apoptotic $CD8^+T$ cells correlated with their Fas expression. Serum TGF-${\beta}1$ levels correlated inversely with $CD3^+Foxp3^+T$ cells. Conclusions: Fas/FasL interactions might lead to excessive turnover of $CD8^+T$ cells and reduce anti-tumor immune responses in patients with HCC. Further investigations of apoptosis induction in $Fas^+CD8^+T$ cells in vitro are required.

• Asian Pacific Journal of Cancer Prevention
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• 제16권10호
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• pp.4215-4218
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• 2015

Gastric cancer (GC) is one of the most common malignancies in the world. It is the first cause of cancer deaths in both sexes In Iranian population. Circulating insulin-like growth factor-one (IGF-1) levels have been associated for gastric cancer. IGF-1 protein has central roles involved in the regulation of epithelial cell growth, proliferation, transformation, apoptosis and metastasis. Single nucleotide polymorphism in IGF-1 regulatory elements may lead to alter in IGF-1expression level and GC susceptibility. The aim of this study was to investigate the influence of IGF-1 gene polymorphism (rs5742612) on risk of GC and clinicopathological features for the first time in Iranian population. In total, 241 subjects including 100 patients with GC and 141 healthy controls were recruited in our study. Genotypes were analyzed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay with DNA from peripheral blood. The polymorphism was statistically analyzed to investigate the relationship with the risk of GC and clinicopathological properties. Logistic regression analysis revealed that there was no significant association between rs5742612 and the risk of GC. In addition, no significant association between genotypes and clinicopathological features was observed (p value>0.05). The frequencies of the CC, CT, and TT genotypes were 97%, 3%, and 0%, respectively, among the cases, and 97.9%, 2.1%, and 0%, respectively, among the controls. CC genotype was more frequent in cases and controls. The frequencies of C and T alleles were 98.9% and 1.1% in controls and 98.5% and 1.5% in patient respectively. Our results provide the first evidence that this variant is rare in Iranian population and it may not be a powerful genetic predisposing biomarker for prediction GC clinicopathological features in an Iranian population.

• 대한간호학회지
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• 제16권1호
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• pp.67-80
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• 1986

The use of intravenous solutions for fluid replacement has become an integral part of patient care, This widespread use of intravenous solutions has increased the risk of contamination that can lead to septicemia and phlebitis. The literature regarding contamination of in use intravenous solutions recommends a standard 24-hour time limit on the use of these fluids. But the desings of these studies did not incorporate a time variable related to contamination. In other studies, however, time was a manipulated variable: but data regarding the onset of contamination were conflicting. Because published reports conflict with regard to a time standard related to the use of intravenous therapy, additional empirical data are needed upon which to base the standards of care regulating use of intravenous therapy. This study investigated rate of contamination in simulated in-use intravenous solutions to obtain data from which to recomend a standard time period for the administration of intravenous solutions. In this study samples were drawn from 60 bottles of 5% D/W solution at predetermined time intervals over 48 hours and samples were inoculated to Thio-glychollate Broth. After 10 days' culturing in that Broth, samples were cultured on blood agar plates for 18∼48 hours to determine the rate of contamination. was found at all time Period, regardless of the presence or absence of nurse's gloving in the preparation of fluids, the location in which the experimentations were performed, the contamination level of surrounding air, or the length of time during which solutions were opened. Data from this study support the use of a 48-hour time period on which to base the standard involved in ready-to-use simple intravenous solutions without additives. In emergency departments and critical care areas where intravenous solutions are prepared in advance, the suggested time standard supported by the data generated from this study is 48 hours, not 24 hour. Data from this study support a 24-hour time standard for changing in-use intravenous solutions when the contamination results from the manipulation of intravenous infusion system by hospital personnel, or from some other exogenous sources during administration. Because contamination that does occur within 48 hours in intravenous solutions must be introduced from some exogenous sources, further empirical studies based on the identification of sources of contamination and factors that affect the rate of contamination, are needed to investigate the currently employed standard of intravenous therapy and to provide the patient with more efficient and safer intravenous thereapy.

• Archives of Plastic Surgery
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• 제33권4호
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• pp.433-439
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• 2006

Purpose: DNA in most cell is regularly damaged by endogenous and exogenous mutagens. Unrepaired damage resulted in apoptosis or may lead to unregulated cell growth and cancer. Inheritance of genetic variants at one or more loci results in an reduced DNA repair capacity. These polymorphisms are highly prevalent in the population, and therefore the attributable risks for cancer could be high. Several studies have documented that polymorphisms of XRCC1, XPD and XRCC3 are associated with skin cancer, especially, XRCC1 among of them has been reported frequently. So, this study involves the relationship between mutation of XRCC1 of squamous cell and basal cell cancer of the skin and risk of cancer development in Korean population. Methods: In case control study, study population (n=100, each cancer) is patients who were pathologically diagnosed as skin cancer(squamous cell carcinoma and basal cell carcinoma) in Yonsei Wonju Christian Hospital and Bundang CHA General Hospital between 1998 and 2004. The samples of DNA from whom no history of premalignant skin lesion and other malignant diseases were reported belonged to the control group(n=210). Blood and tissue samples were analyzed for presence of XRCC1 Arg399Glu, Arg280His, Arg194Trp using PCR/ RFLP method. Results: For Korean, there was a significant correlation between XRCC1 Arg399Gln gene mutation and risk of basal cell carcinoma development(Arg 399Gln(GA), p=0.012, OR=2.016, 95% CI; 1.230-3.305) /Arg399Gln (AA), p=0.011, OR=1.864, 95% CI; 1.149-3.026)). And, there was also significant correlation between XRCC1 Arg194Trp and risk of skin squamous cell carcinoma development (Arg194Trp (CT+TT), p=0.041, OR=0.537, 95% CI; 0.301-0.960)). In contrast, there was no significant correlation between XRCC1 Arg280His and risk of either basal cell carcinoma or squamous cell carcinoma development. Conclusions: Our result present that XRCC1 Arg399 Gln in basal cell carcinoma and XRCC1 Arg194Trp in squamous cell carcinoma have possibility of cancer risk and biomarker in Korean population. But XRCC1 Arg280 His known having cancer risk on other studies is not associated with cancer risk to squamous cell carcinoma and basal cell carcinoma in Korean population.

• Applied Microscopy
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• 제23권1호
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• pp.35-55
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• 1993

cis-Dichlorodiammineplatinum (II) (cis-Platin), a metallic compound, has widely been used as an effective anticancer chemotherapeutic agent. The precise mechanism of action of this agent is still unknown, but it is postulated that cis-Platin may act on the cancer cell like bifunctional alkylating agents. Although this agent is very beneficial to the patients with cervical cancer, germinoma of testis, neuroblastoma and others, it may also damage to the normal cell so that many side effects; severe hemorrhagic enterocolitis, bone marrow depression, renal damage and liver damage will develope. This experiment has been undertaken to pursue the cytotoxic effects of the cis-Platin on the ultrastructures of the interalveolar septum in the mouse lung. A total of 55 healthy male mice of ICR strain were used as experimental animals and divided into 5 mice of normal control group and 50 mice of cis-Platin treated group. The mice of cis-Platin treated group were sacrificed by carotid exsanguination at 6, 12, 24 hours, 3 days and 7 days after intraperitoneal injection of 6.0 mg of cis-Platin ($Abiplatin^R$ Abic Co. Ltd.) per kg of mouse body weight. The specimen obtained from the lower lobe of left lung were sliced into $1mm^3$ and prefixed with 2% glutaraldehyde -2.5% paraformaldehyde solution prepared with Millonig's phosphatae buffer solution (pH 7.4) at $4^{\circ}C$ for 3-4 hours. After postfixation with 1% osmium tetroxide solution all specimens were embedded in Epon 812. Ultrathin sections about $600-800{\AA}$ in thickness were stained with uranyl acetate and lead citrate and observed with Hitachi-600 electron microscope. The results obtained were as follows: 1. Local swellings with increase of electron density and number of pinocytic vesicles in the cytoplasms of the type I pneumocyte and endothelial cell of the blood air barrier in interalveolar septum of cis-platin treated mice were observed. 2. Cisternae of rough endoplasmic reticulum were dilated and sacculated in association with detachment of membrane bound ribosomes of the type II pneumocyte in interalveolar septum of cis-Platin treated mice. 3. Swollon mitochondria with uneven electron density of their matrix were observed in the type II pneumocyte of interalveolar septum in the cis-Platin treated mice. 4. The lamellae of lammelar bodies in type II pneumocyte of interalveolar septum in cis-Platin treated mice were devoided or transformed into homogeneous electron dense material. It is consequently suggested that cis-Platin would induce the cellular edema of type I pneumocyte and endothelial cell, and degenerative changes of cytoplasmic organelles of the type II pneumocyte in the interalveolar septum of the mouse lung.

• Parasites, Hosts and Diseases
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• 제55권5호
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• pp.481-489
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• 2017

Tick-borne pathogens can cause serious problems in grazing cattle. However, little information is available on tick-mediated diseases in cattle grazing on mountains. Thus, this study aimed to understand the potential problems related to tick-borne diseases in grazing cattle through the investigation of prevalent tick-transmitted infections, and their associated hematological changes, in terms of season and grazing type in Korean indigenous cattle (=Hanwoo). Hanwoo cattle from 3 regions of the Republic of Korea (=Korea) were either maintained indoors or placed on grassy mountains from spring to fall of 2014 and 2015. Cattle that grazed in mountainous areas showed a greater prevalence of tick-borne infections with an increased Theileria orientalis infection rate (54.7%) compared to that in non-grazing cattle (16.3%) (P<0.001). Accordingly, the red blood cell (RBC) count and hematocrit (HCT) values of grazing cattle were significantly lower than those of non-grazing cattle throughout the season (P<0.05). Moreover, RBC, hemoglobin (Hb), and HCT of T. orientalis-positive group were significantly lower than those of T. orientalis-negative group (P<0.05). T. orientalis is a widespread tick-borne pathogen in Korea. Grazing of cattle in mountainous areas is closely associated with an increase in T. orientalis infection (RR=3.4, P<0.001), and with consequent decreases in RBC count and HCT. Thus, these findings suggest that the Hanwoo cattle in mountainous areas of Korea are at a high risk of infection by T. orientalis, which can lead to hematological alterations. This study highlights the necessity of preventive strategies that target T. orientalis infection.

• 문화기술의 융합
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• 제3권4호
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• pp.1-19
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• 2017

죽음의 문화는 삶의 문화의 반쪽이라는 의미에서 상보적(相補的)이다. 3개의 이승 정낭과 2개의 묘(墓)의 저승 신문(神門)은 올레길 공간체로 연결되어 있다. 그 공간체에는 삶과 죽음사이의 상생(相生)과 상극(相剋)이 공존하는 상보성(相補性)(complementarity) 원리가 제주 문화(文化)에 숨어있다. 대(對)와 대(待)이다. 즉 반대되는 것은 서로 보완적이다란 말이 "(Contraria Sunt Complementa 라틴어)" 서로 대립하면서도 서로 의존하는 관계로 서로가 서로를 품은 관계를 뜻한다. 정낭은 통신 원리로 사용될 뿐 아니라 인체의 RNA Codon에 기본 원리로 사용된다. 또한, 묘의 사각형 산담 귓돌과 한국의 태극과 괘(卦), 유전자(RNA)의 괘(卦), 연결고리의 유사성 Pattern을 들 수 있다. 제주에는 흑용만리 곡선밭담과 사각형 산담이 들판에 펼쳐있다. 제주에서 돌담은 괸담(Stone Networks)으로 연결되고, 괸담의 관습상 발음이 되는 괸당은 친족(Relative Family Networks)로 연결된다. 조상의 명당 묘와 자손들 관계는 영혼적으로 동기감응(同氣感應: Soul Synchronizing the Ancestor to Offspring)이 되어 발복(發福: Change in Future)이 된다고 믿고, 육체적인 피(血)인 유전인자가 자식들에게 직접 전수된다. DNA RNA를 행렬식으로 표시했다.