• Toxicological Research
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• v.17
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• pp.135-138
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• 2001

Female Wistar rats were randomly divided into 5 groups: Control, received distilled water; Low lead, received 0.5 g/ιlead (as acetate) in drinking water; High lead, received 2.0 g/ιlead; Low lead + Minerals, received 0.5 g/ιlead in drinking water and received minerals (Ca$^{2+}$, 25 mg/kg/day; Fe$^{3+}$, 0.47 mg/ kg/day; Zn$^{2+}$, 0.33 mg/kg/day; Se, 0.83 $\mu\textrm{g}$/kg/day) by gavage; High lead + Minerals, received 2.0 g/ιlead and received the same minerals. Animals exposure to lead was from 10 days before mating till postnatal day 21; and the minerals was administered from the first day of pregnancy and during lactation. No statistical difference was found either in body weights or in blood lead levels between the pups received minerals and those only exposed to lead at the same dose. The developmental and behavioral teratological effects of lead on pups, such as time-lag of eye opening, pinna detachment, fur developing, incisor eruption, ear unfolding, and surface righting were observed in this study; and the minerals decreased the toxicity of lead either in low or in high lead exposure pups. The numbers of step-down were significantly increased in lead exposed animals, and the effect of intervention by the minerals was appeared only in the pups exposed to low lead. The ChAT activity and levels of glutamate and aspartate in hippocampus decreased in treated animals compared to control animals, no effect of intervention by the minerals was found. The results of this study indicate that the applied multi-minerals can alter the outcome of develop-mental lead poisoning in rats.s.s.s.

• Environmental Mutagens and Carcinogens
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• v.15 no.2
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• pp.122-130
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• 1995

In order to evaluate the cytotoxicity of lead in cultures of Balb/c mouse 3T3 cell line, various cytotoxic assays were carried out after expose cells to various concentrations of lead nitrate. Cytotoxic assays using this study were included NR assay, MTT assay, measurement of LDH and protein, synthetic rate of DNA and UDS. Intrace!!ular Ca$^{2+}$ level was also measured. Light and electron microscopic studies were done for morphological changes of lead-treated cell cultures. The results were as follows; 1. The absorbances of NR and MTT were decreased dose-dependently, and NR, and MTT, values of lead nitrate were 3.4 mM and 1.5 mM, respectively. 2. Amount of LDH released into the medium was increased in dose-dependently and LDH activity at 5 mM concentration of lead nitrate was increased to 335 % of control. 3. Amount of total protein was decreased dose-dependently, and which was half of control at 2 mM concentration of lead nitrate. 4. The synthetic rate of DNA was decreased dose-dependently, and also which was remarkably decreased at 3 mM and 5 mM concentrations of lead nitrate. 5. The synthetic rate of UDS was increased at 1 mM concentration of lead nitrate, but which was remarkably decreased at 3 mM and 5 mM concentrations of lead nitrate. 6. Intrace!lular Ca$^{2+}$ level was remarkably increased at 1 mM concentration of lead nitrate, compared with control. 7. In light microscopy, number of cells and processes were decreased according to the increase of dosage of lead nitrate. Electron microscopic findings showed that many vacuoles and cisternal dilatation of rough endoplasmic reticulum were seen in the cytoplasm at 1 mM concentration of lead nittale. From the above results, high dosage treatment of lead nitrate (>3 mM) damaged genetic malerials and it also showed cytotoxicity in mouse 3T3 cell line cultures by injury of cell organelles and Ca$^{2+}$ channel.

• Korean Journal of Environmental Agriculture
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• v.15 no.3
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• pp.306-315
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• 1996

This study was performed to develop the biological treatment technology of wastewater polluted with heavy metals. The lead-tolerant microorganism, Pseudomonas aeruginosa which possessed the ability to accumulate lead, was isolated from the industrial wastewater polluted with various heavy metals. The characteristics of lead accumulation in the cells and the recovery of the lead there from were investigated. Removal rate of lead from the solution containing 100mg/l of lead by the lead-tolerant microorganism was more than 97% at 48 hours after inoculation with the microorganism. A large number of the electron-dense granules were found mainly on the cell wall and cell membrane fractions, when determined by transmission electron microscopy. Energy dispersive X-ray spectroscopy revealed that the electron-dense granules were lead complex with the substances binding heavy metals. The lead accumulated into cells was not desorbed by distilled water, but more than 87% of the lead accumulated was desorbed by 0.1M-EDTA. The residues of the cells after combustion at $550^{\circ}C$ amounted to about 30% of the dry weight of the cells. EDS analysis showed that the residues were relatively pure lead compounds containing more than 86% of lead.

• Journal of Plant Biology
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• v.20 no.1
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• pp.7-14
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• 1977

The seeds of bean(Glycine max M.) and corn(Zea mays L.) soaked in 5000ppm lead solution for 24 hours were sowed in the flowerpots being placed sandy-clay soil under the field condition. The fixed concentrations of various anions and 2000 ppm lead were supplied alternately in the sandy-clay soi of the flowerpots at two days interval from May to July in 1976. After the plants were harvested prior to the flowering stage, the lead contents of plnat and soil were analyzed by atomic absorption spectrophotometer. The lead contents absorbed by the plant roots showed the highest in an weak acid soil of the best suitable condition of plant growth. The absorption of lead by the plant roots was inhibited by the various anions, especially divalent anions of the soil. Some phosphate anions inhibited lead absorption more than otehr various anions in the soil. The more various anions were in the soil, the more plants could be protected from the lead toxicity. In the case of lead supply in the soil, 99.5% of lead was accumulated in the upper layer of the soil(0-10cm), and 0.5% of lead accumulated in the lower layer (10-20cm). Therefore, the yellow-brown and white symptoms on the leaves and the inhibition of root growth by lead toxicity was increased in the early stage of the germination, however decreased in accordance with the progress of the growing stage becuase of the root growth toward beneath the lower layer of the soil. In spite of the contents of 3773ppm lead in the soil, the symptoms of lead toxicity was not found in the grown plants. At that time the lead contents of the plants absorbed from the soil were minimum 0.78ppm and maximum 3.64ppm through the growing stage.

• Journal of Environmental Health Sciences
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• v.19 no.3
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• pp.46-51
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• 1993

In order to survery the risk of air-borne lead to human, the relation between air-borne lead level and blood lead level was examined by using of the kinetic model and statistical model. The results of this survey were as follows: 1. The pathways of lead intake were food and water, mainly. 2. Though blood lead level of Korean urbanire was higher than that of American or Japanese, it was not so severe as to influence human health. 3. The lead content in food and water was high, and so it is needed to confirm the cause of high content was whether second contamination by air pollution or not.

• Economic and Environmental Geology
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• v.57 no.3
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• pp.343-352
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• 2024

Composition analysis and lead isotope ratio analysis were conducted to determine the coloring machanism on lead glaze used in Gyeongbokgung Palace and the provenance of the lead used as a flux. 31 blue tiles were classified into green, blue, and yellow. The chemical analysis of lead glazes on the blue tiles revealed that Pb, Si, and Cu were the main components, and trace amounts of Fe, Ca, Mg, and Al were detected. The Cu content was high in blue lead glaze, while Cu was not detected in yellow or brown lead glaze which instead had high Fe content. Therefore, it was found that lead was used as a flux and copper oxide as a coloring agent in the production of lead glaze. In addition, the lead isotope ratios of the lead glaze used in the blue tiles of Gyeongbokgung palace were plotted in zone 3 on the distribution map of lead isotope ratios on the Korean Peninsula, which includes Chungcheong-do and Jeolla-do. It is presumed that the flux for the lead glaze was sourced from galena found in these regions. The lead isotope ratios of the green glaze from the Three Kingdoms and Unified Silla period were mostly located outside the Korean Peninsula, showing that the provenance of lead had changed. In particular, the lead isotope ratios of the green glaze from the Three Kingdoms and Unified Silla period suggest exchange with neighboring countries. Also the lead isotope ratios of the green glazes from the same temple are different, so it is believed that they were made at different times or in different workshops.

• Journal of Welding and Joining
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• v.18 no.1
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• pp.70-76
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• 2000

A rolled steel for structural parts and lead bronze alloy were bonded each other by a new semi-solid diffusion bonding process to investigate the effect of the process parameters, for example bonding temperature and bonding time, on the interface characteristics, and bonding behavior. It can be possible that manufacture of the bonded steel/lead bronze which has a cylindrical shape with inserted the lead bronze alloy into the steel ring by the diffusion bonding process under the semi-solid condition of the lead bronze alloy without any pressure and flux. It has been know that the control of the amount of the liquid phase in semi-solid lead bronze alloy was very important to obtain soundness interface, since the shear strength of the bonded steel/lead bronze at 850℃ for 60 minutes under the condition of about 40% of the liquid phase in the lead bronze alloy shows maximum value, 210 MPa. The shear strength increases with an increase in bonding time and show maximum value, and then decreases.

• Journal of Environmental Health Sciences
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• v.27 no.1
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• pp.83-87
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• 2001

In this study, the characteristics of lead removal by PVA and alginate bead which used widely as immobilizing agents were investigated, and the difference of removal amounts between pure PVA/alginate bead and Sargassum thunbergii immobilized bead was studied. All PVA beads, pure and S. thunbergii immobilized, reached an equilibrium state in about 1 hour, and S. thunbergii immobilized bead adsorbed more lead than pure one. But in the case of alginate beads, they needed much time, about 5 hours, to reach an equilibrium state, and adsorbed lead four times higher than PVA beads. Therefore, it was considered that alginate beads had more mass transfer resistance and function groups which adsorb lead such as hydroxyl, carboxyl and etc. than PVA bead. To examine the continuous usage of alginate beads, the process of adsorption/desorption of lead was conducted repeatedly. As the process proceeded, the amounts of lead adsorption decrease, so it was indicated that the non-desorbed lead from alginate bead at first adsorption/desorption process remained constantly.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2001.07a
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• pp.628-631
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• 2001

Current lead is one of the first proposed devices for the application of High Temperature-Superconductor(HTSC). The current lead provides high current for electrical machine using superconductor from room temperature. Its characteristics that is zero resistance and low heat transfer rate under critical temperature lead to research for the replacement of existing current lead with HTSC. In this paper, we investigated the temperature distributions of stacking type and rod type current lead with each cross-section area and length using Nastran program and compared each temperature distribution. It is obtained from this paper that stacking type current lead has flat temperature gradient and than rod type one and more stable operation as current lead is closely related with its cross-section area and length.

• Journal of environmental and Sanitary engineering
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• v.16 no.3
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• pp.14-21
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• 2001

The effects of sodium molybdate(Mo) on phospholipid metabolism in sciatic nerve isolated from lead-intoxicated rat were investigated. Four weeks aged albino rats were divided into four groups : normal control group, lead(10 ppm)-treated group, Mo(1 mg/kg)-treated group, lead and Mo-treated group. As a result of the study, Mo significantly increased the rate of incorporation of $2-[^3H]$myo-inositol into polyphosphinositides in lead-intoxicated rat. Mo also increased the rate of phosphatidylcholine metabolism in normal and lead-intoxicated rats. However, Mo did not affect the metabolism of phosphatidylserine and phosphatidylethanola-mine in normal and lead-intoxicated status. These results suggest that Mo might improve lead-intoxicated status by principally enhancing the metabolism of myo-inositol-related phospholipids and by partly phosphatidylcholine in sciatic nerve.