• Journal of the Korean Society of Environmental Restoration Technology
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• v.8 no.4
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• pp.12-22
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• 2005

The purpose of this study is to select suitable planting base for the mat-type rooftop greening in order to popularize rooftop greening system easily. The experiment was conducted from 2004 June to 2005 May under several conditions; 4 soil depths under mats(2cm, 5cm, 10cm, 15cm), two soil mixtures(natural soil 80%+leaf mold 20%, artificial soil) and two light conditions(full sun place, 20% shaded place). In this experiment, 3 types of mats were used ; the herbaceous plants mat(11 plants inclusive of Lotus corniculatus L., Silene armeria L.), the lawn mat with Festica arundinacea and Sedum mat with Sedum kamtschaticum, Sedum sarmentosum, Sedum oryzifolium, Sedum middendorffianum. The result is as follows; in the mat-type rooftop greening, the herbaceous plants mat, lawn mat and sedum mat are the similar number of plant and effect of greening on soil depth 2cm, 5cm and 10cm, 15cm. So suitable soil depth of rooftop greening is 10cm for the load and economical factor. Thus the mat-type rooftop greening possible planting base depth of all 13cm as soil depth 10cm and mat depth 3cm. As soil mixtures, the number and growth of plants were better mat and 'natural soil 80% +leaf mold 20%' than mat and artificial soil. In herbaceous plants mat, Silene armeria L., Dianthus chinensis, Centaurea cyanus L., Lotus corniculatus L. are survival in full sun place and Silene armeria L., Dianthus chinensis, Centaurea cyanus L. are survival in 20% shaded place. In conclusion, selection of suitable soil mixtures and plants is possible extensive management rooftop greening with effect of continuous greening. The mat-type rooftop greening are lightweight and simple preparation without management and can popularize readily.

• Journal of Korean Society of Environmental Engineers
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• v.27 no.12
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• pp.1257-1262
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• 2005

Organo-chlorinated pesticides have been seldom measured in Korea because their use was banned for more than two decades. Nevertheless, the environmental persistency of these pesticides requires regular monitoring. Only organo-phosphorus pesticides are currently being monitored in Korea. Simultaneous determination of organo-chlorinated and organo-phosphorus pesticides was attempted using gas chromatography/mass spectrometer detector. Pressurized fluid extraction(PFE) was used in sample preparation. PFE showed equivalent extraction efficiency to traditional soxhlet extraction with less solvent use and less sample preparation time. Average recovery of spiked pesticides to soil and lawn samples was 62% ranging 52 to 154%. Method detection levels(MDLs) for these pesticides ranged from 3.4 to 10.5 ${\mu}g/kg$. Endosulfan, fenitrothion, and diazinon were found in the samples from 5 different golf courses out of 9 courses.

• Korean Journal of Weed Science
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• v.32 no.2
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• pp.139-143
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• 2012

This experiment was carried out to investigate the growth responses of white clover (Trifolium repens L.) as affected by prohexadione-calcium foliar application for the growth restraint in the lawn yard, field and golf course etc. The leaf length and leaf weight of white clover were significantly retarded compared to the untreated plot with foliar application of prohexadione-calcium after mowing within 1 day, and the effect was continued up to 50 days. The leaf area of clover with prohexadione-calcium treatment was to be narrowed as compared to untreated plot. The SPAD value of the leaf colour was increased in the treated plots and was to be continued for 40 days more. The coverage rate of clover was decreased with prohexadione-calcium foliar application after mowing in the lawn ground.

• Korean Journal of Organic Agriculture
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• v.20 no.4
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• pp.589-605
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• 2012

This study was conducted to identify allelopathic effect of Ganghwa domestic Artemisia spp., named Sajabalssuk and Ssajuarissuk, for various receptor plants including clover (Trifolium repens L.), alfalfa (Medicago sativa L.), lawn grass (Zoysia japonica Steud.), dandelion (Taraxacum platycarpum Dahlst.), and dahurianpatrinia (Patrinia scabiosaefolia Fisch. ex Trevir). Receptor plants were treated with the aqueous and essential oil extract of Artemisia plants. In consequence, their allelopathic effects were evaluated by measuring seed germination rates, seedling growth, and dry weights of the receptor plants. The seed germination and seedling growth of the receptor plants were inhibited by all treatments of both aqueous and essential oil extracts of the Artemisia plants, and, in addition, the inhibitory effects were increased according to the higher concentration. Among the donor plants, A. $sp.^*III$ showed most effective allelopathic effect. Comparing the alleopathic effect among the receptor plants, seed germination was most inhibited in lawn grass while inhibitory effect of seedling growth was comparatively higher in dandelion. Although inhibitory effects were comparatively lower, the allelopathic effects of Artemisia plants were identified in clover and alfalfa since the seedling growth of these plants were inhibited more than 70%. Thus, in result, Ganghwa domestic Artemisia spp. could be possibly used for weed control since natural products of the plants showed inhibitory effects on seed germination and seedling growth of various receptor plants.

• Journal of Korean Society on Water Environment
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• v.25 no.6
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• pp.886-895
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• 2009

The guideline of selection of Integrated Management Practices (IMPs), such as wood, green roof, lawn, and porous pavement, for Low Impact Development (LID) design was proposed by ranking the reduction rate of surface runoff using LIDMOD1.0. Based on the guideline, LID was designed with several scenarios at two apartment complexes located at Songpa-gu, Seoul, Korea, and the effect of LID on surface runoff was evaluated during last 10 years. The effect of runoff reduction of IMP by land use change was highly dependent on the kind of hydrologic soil group. The wood planting is the best IMPs for reduction of surfac runoff for all hydrologic soil groups. Lawn planting is an excellent IMP for hydrologic soil group A, but reduction rate is low where soil doesn't effectively drains precipitation. The green roof shows constant reduction rate of surface runoff because it is not influenced by hydrologic soil group. Compared to the rate of other IMPs, the green roof is less effect the surface runoff reduction for hydrologic soil group A and is more effect for hydrologic soil group C and D followed to planing wood. The porous pavement for the impervious area is IMPs which is last selected for LID design because of the lowest reduction rate for all hydrologic soil group. As a result of LID application at study areas, we could conclude that the first step of the strategy of LID design at apartment complex is precuring pervious land as many area as possible, second step is selecting the kind of plant as more interception and evapotranspiration as possible, last step is replacing impervious land with porous pavement.

• Korean Journal of Veterinary Research
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• v.33 no.3
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• pp.393-406
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• 1993

Antibacterial substances produced by Lactobacillus casei subsp. and Streptococcus faecium were examined for its antibacterial effects against some pathogenic bacteria. They were partially purified with ammonium sulfate precipitation, methanol-acetone extraction, G-50 gel filtration and examined its characteristics. When L casei subsp. and Str faecium were cultivated in MRS broth, stationary phase of L casei is until 24 hours and Str faecium is 20 hours. pH change of the cultured medium was both decreased after 12 hours and then constant at pH 4.5~4.6 after 28 hours. MRS broth culture fluids of L casei subsp. and Str faecium appeared the antibacterial effects by the spot-on-the-lawn method against ETEC, Sal pullorum and Sta aureus. Culture filtrates of L casei subsp. and Str faecium also appeared the antibacterial effects by the disc diffusion method. Culture filtrates of L casei sub. rhamnosus 7469 produced 0.032M of lactic acid and 0.01M of acetic acid. Str faecium 27273 also produced 0.027M of lactic acid and 0.01M of acetic acid. Protein concentrations of culture filtrates produced by L casei sub rhamnosus 7469 and Str faecium 27273 was $495{\mu}g/m{\ell}$ and $594{\mu}g/m{\ell}$, respectively. Antibacterial substances which are partially purified by ammonum sulfate precipitation, methanol-acetone extraction and G-50 gel filtration inhibit the growth of ETEC, Sal pullorum and Sta aureus. Characteristics of purified antibacterial substances was examined. Its molecular weight was about 31Kd, stabilized at $100^{\circ}C/20min.$ and some of proteolytic enzyme treatment.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.1
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• pp.1-7
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• 2003

In spite water direct seeding cultivation of rice has advantages in terms of laborsaving and cost-down, it demonstrates uneven seedling establishment and difficult weed management. To select an applicable mat for mulching system of water seeded rice, weed occurrence, growth and yield performances of rice, and soil temperature were evaluated in several kinds of biodegradable mulching material. Field studies were conducted at the experimental farm of National Crop Experiment Station in 1999 and 2000. Among the mats tested in 1999, Safer-mat had the greatest effect on inhibiting weed occurrence and Lawn-mat demonstrated the least efficacy of weed control (EWC) value. In Safer-mat treatment, Ludwigia prostrata was the dominant weed species but Echinochloa crus-galli was not observed. Different kinds of mulching material from the mats studied in 1999 were tested in 2000. Biodegradable polyester (PES) coated on the recycled paper for newspaper press (RP) treatments showed similar or higher EWC values than non-mulched broadcast seed rice with 2 times of herbicide application (BC-herbicide) and Safer-mat that was the most effective mat on preventing weed appearance in 1999. Immediately after mat mulching on the surface of paddy soil, five pregerminated rice seeds were manually sown in each 3cm-hole formerly punctured at 30${\times}$14 cm spacing in 2000. All the 3 kinds of mulching material tested in 2000 had better seedling stand than BC-herbicide treatment. Rice yield in PES coated with 10 thickness on RP (PES10 $\mu\textrm{m}$＋RP) was a little higher than in BC-herbicide treatment but the former produced less panicles in unit land area than the latter. PES10 $\mu\textrm{m}$＋RP demonstrated a great effect on raising soil surface temperature than non-mulching treatment throughout the period of seedling establishment.

• Asian Journal of Turfgrass Science
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• v.2 no.1
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• pp.5-30
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• 1988

The object of this experiments was to know the salt tolerance of Fults and other lawn grasses. Fults, Olympic (Tall fescue, Festuca arundinacea Schred), Ceeping red fescue, Kentucky bluegrass and Zoysia grass (Z. japonica S.) were grown in hydroponics with vermiculite at various concentrations of NaCl. Hoagland's solution was used as the basic solution (control), and the concentrations of Cl to it were 1000, 2000, 3000, 4000 and l5OOOppm, respectively. Each was cultivated under the circumstances during 2 months. The results obtained are summarized as follows: 1.The growth of Fults, Olympic, Creeping red fescue and others were better at Cl lOOOppm than control. In the 5OOOppm application, Fescues become worse and 23.9% of them were withered. In concentration of Cl above 9OOOppm, it was impossible to live. 2.In the l0000~l1000ppm application, Olympic and Kentucky bluegrass were become worse and most of them died. 3.Fults were almost possible to live in the below of 9OOOppm, but they began to die in the above of 10000 ppm. 4.With the increasing concentration of Cl, plants were dwarfed and the number of stems, leaves and roots were reduced, but it was especially observed that the number of stolons of Creeping red fescue were increased at 1000~4000ppm. 5.Fults grass was the most salt tolerant turfgrass, but was impossible to live at salt level of about 36 millimhos (Exchange NaCI conductivity-ppm). Among the grasses, according to salt tolerance, they were arranged as follows. Fults > Zoysia japonica S. > Ky belugrass > Olympic grass > Creeping red fescue 6.The number of leaves, stems, tillers, and dry weight of Olympic grass, Fults and others were increased more at Cl 1OOOppm application than control, but in the above 4OOOppm application, those of plants were decreased. 7.The productivity of all grasses under the experiments was increased at 3.l75millimhos (Exchange NaCi conductivity ppm) in the concentration of Cl. The each dry-weight of Olympic, Creeping red fescue, Kentucky bluegrass and Zoysia grass was decreased at 8.85millimhos, and the weight of Fults grass was also decreased remarkably at 12.20millimhos. 8.As the result of this experiments, most plants grow normally at low concentration of NaCI-l000ppm. That seems to stimulate more the grasses to grow than non-salt.

• Asian Journal of Turfgrass Science
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• v.1 no.1
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• pp.18-29
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• 1987

This study was carried out to examine the effect of nitrogen application and clipping interval on the characteristics of several turf components of korean lawngrass for the basic data of lawn management. It was treated by Split plot design with three replications. The main plots were nitrogen levels with 0, 350, and 700kgN / ha, and the sub plots were clipping intervals with 10, 20, and 30 days The results obtained are summarized as follows ; 1. Increasing the rate of nitrogen fertilizer and frequent clipping increased tiller number of korean lawngrass and the maximum number of tillers obtained in October were recorded from 700kgN application and clipping treatment of 10 days interval. Meanwhile, treatment of 350kgN with 10 days clipping interval increased tillers much more than those of 700kgN with 20 and 30 days clipping intervals. 2. The average number of green leaves occurred during the growth period maximized by applying 700 kgN and clipping 10 days interval. 3. Increasing tiller numbers significantly decreased tops DM weight per tiller by clippng plants at interval of 10 and 20 days, irrespective of nitrogen applied, and with nil N, at the interval of 30 days. By applying 700kgN however, tops DM weight per tiller increased as the number of tillers increased consistently. 4. The highest tops DM weight was achieved from late August to early September by applying 350 and 700kgN. 5. During the growth period, nitrogen application increased unders(stolon+root) DM weight, and, at the same level of nitrogen applied, the increase in stolon DM weight enhanced by lengthening the clipping interval to 30 days. 6. Nitrogen efficiency to green leaves, stolon nodes and DM weight of root with high nitrogen was achieved as clipping interval was shortened.

• Horticultural Science & Technology
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• v.28 no.4
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• pp.648-655
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• 2010

This study is the first comprehensive report on the molecular cloning, structural characterization, sequence comparison between wild and mutant types, copy number in the genome, expression features and activities of a gene encoding 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) in Korean lawn grass ($Zoysia$ $japonica$). The full length cDNA of the EPSPS from Korean lawn grass ($zj$EPSPS) obtained from a 3' and 5' RACE method was 1540 bp, containing a 1176 bp ORF, a 144 bp leader sequence (5' UTR) and a 220 bp 3' UTR, which was eventually decoded 391 amino acid residues with a molecular mass of 41.74 kDa. The Southern blot detection of the $zj$EPSPS showed that the gene exists as a single copy in the Korean lawn grass genome. Sequence comparison of the $zj$EPSPS gene demonstrated that the glyphosate-tolerant mutant (GT) having a Pro-53 to Ser substitution in the gene seems to have a preferred binding activity of the enzyme to phosphoenol pyruvate(PEP) over glyphosate, which allows the continuous synthesis of aromatic amino acids in the shikimate pathway. From the Northern blotting analysis, the $zj$EPSPS was found to be highly expressed, with continuous increase until 36 hours after 0.5% glyphosate treatment in both wild and mutant samples, but 1.5-fold higher EPSP synthase activity was observed in the tolerant mutant when exposed to the glyphosate treatment. The molecular information of the $zj$EPSPS gene obtained from this study needs to be further dissected to be more effectively applied to the development of gene-specific DNA markers and zoysiagrass cultivars; nevertheless, the glyphosate-tolerant mutant having the featured $zj$EPSPS gene can be provided to turfgrass managers for weed problems with timely adoptable management options.