The effect of the shape of the side wall on vaporization and fuel mixture were investigated for the impinging spray of a direct injection(DI) gasoline engine under a variety of conditions using the LIEF technique. The characteristics of the impinging spray were investigated under various configurations of piston cavities. To simulate the effect of piston cavity configurations and injection timing in an actual DI gasoline engine, the parameters were horizontal distance from the spray axis to side wall and vertical distance from nozzle tip to impingement plate. Prior to investigating the side wall effect, experiments on free and impinging sprays for flat plates were conducted and these results were compared with those of the side wall impinging spray. For each condition, the impingement plate was located at three different vertical distances(Z=46.7, 58.4, and 70 mm) below the injector tip and the rectangular side wall was installed at three different radial distances(R=15, 20, and 25 mm) from the spray axis. Radial propagation velocity from spray axis along impinging plate became higher with increasing ambient temperature. When the ambient pressure was increased, propagation speed reduced. High ambient pressures tended to prevent the impinging spray from the propagating radially and kept the fuel concentration higher near the spray axis. Regardless of ambient pressure and temperature fully developed vortices were generated near the side wall with nearly identical distributions, however there were discrepancies in the early development process. A relationship between the impingement distance(Z) and the distance from the side wall to the spray axis(R) was demonstrated in this study when R=20 and 25 mm and Z=46.7 and 58.4 mm. Fuel recirculation was achieved by adequate side wall distance. Fuel mixture stratification, an adequate piston cavity with a shorter impingement distance from the injector tip to the piston head should be required in the central direct injection system.
Despite of importance of integrated events of nucleus and microtubule remodeling in nuclear transferred embryos with somatic cells, little information is available on this subject. In this study we configured chromatin and microtubule organization following somatic cell nuclear transfer in pre- and non-activated bovine oocytes in order to clearify nuclear remodeling process and to demonstrate centrosome inheritance during nuclear transfer. The cumulus-oocyte complexes were collected from slaughterhouse and were matured in vitro for 20 h in TCM 199 supplemented hormone. Matured bovine oocytes were enucleated by aspirating the frist polar body and metaphase chromatin using a beveled pipette. Bovine fibroblast cells were fused into enucleated oocyte by electrical stimulation. Reconstructed oocytes were activated with ionomycine and 6-dimethylaminopurin, and then cultured in CRlaa medium. The organization of nuclear and microtubules were observed using laser-scanning confocal microscopy. At 1 hour after fusion, microtubule aster was seen near the transferred nucleus in most oocytes regardless activation condition. While most of fibroblast nuclei remodeled to premature chromosome condensation (PCC) and to the two masses of chromosome in non-activated oocytes, a few number of fibloblasts went to PCC and multiple pronuclear like structures in activated oocytes. Microtubular spindle was seen around condensed chromosome. Gamma-tubulin was detected in the vicinity of condensed chromosome, suggesting this is a transient spindle. The spindle seperated nucleus into two masses of chromatin which developed to the pronuclear like structures. Two pronuclear like structures were than apposed by microtubular aster and formed one syngamy like nuclear structure at 15 h following nuclear transfer. At 17 to 18 h after fusion, two centrosomes were seen near the nucleus, which nucleates micrtubules for two cell cleavage. While 31% of reconstructed oocytes in non-activated condition developed to morulae and blastocysts, a few reconstructed oocytes in pre-activated condition developed to the blastocyst. These results suggested introduction of foreign centrosome during nuclear transfer, which appeared to give an important role for somatic cell nuclear reprogramming.
Chifenti, Barbara;Locci, Maria Teresa;Lazzeri, Gloria;Guagnozzi, Mariangela;Dinucci, Dino;Chiellini, Federica;Filice, Maria Elena;Salerno, Maria Giovanna;Battini, Lorella
Clinical and Experimental Reproductive Medicine
/
v.40
no.1
/
pp.33-37
/
2013
Autophagy is a degradation process that acts in response to environmental stressors. Recently, autophagy has been detected in normal term, preeclamptic and intrauterine growth-restricted placentas. The object of this work was to investigate the presence of autophagy in first trimester voluntary interruption of pregnancy placental villi by the expression of autophagy-related proteins, light chain 3 (LC3), and Beclin-1. In first trimester placental villi laser scanning confocal microscopy (LSCM) analysis revealed LC3 and Beclin-1 immunoreactivity prevalently located in villous cytotrophoblasts. Using LSCM, LC3, and Beclin-1 were localized to the cytoplasm of the trophoblast layer in human full-term placentas. Beclin-1 expression and LC3 activation were confirmed by western blotting. These data emphasize that autophagy activation is different among cytotrophoblasts and syncytiotrophoblasts depending on the gestational age and thus we speculate that autophagy might play a prosurvival role throughout human pregnancy.
Journal of the Korea Academia-Industrial cooperation Society
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v.9
no.4
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pp.892-898
/
2008
3D rapid prototyping is the manufacturing technology to fabricate a prototype with the data stored in a computer, which differs from conventional casting technology in terms of an additive process. Various 3D rapid prototyping techniques such as stereolithograpy. fused deposition modeling. selective laser sintering, laminated object manufacturing have been developed but among them, 3D inkjet printing has a unique feature that materials could be jetted to directly form the body of a prototype, which could be a finished product functionally and structurally. However, this needs ink with a high solid content, which tends to increase the dynamic viscosity of ink. The increase of ink viscositytends to restrict the jettable range of ink and hence the jetting conditions should be optimized. The intrinsic speed of sound in a hot melt ink with ceramic nanoparticles dispersed is one of key components to determine the jettable range of ink. In this paper, the way to measure the intrinsic speed of sound in a hot melt ceramic ink is proposed and its influence on the jetting condition is discussed.
Prasad, Y. Nagendra;Kwon, Tae-Young;Kim, In-Kwon;Park, Jin-Goo
Proceedings of the Materials Research Society of Korea Conference
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2011.05a
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pp.34.2-34.2
/
2011
The demand for Ru has been increasing in the electronic, chemical and semiconductor industry. Chemical mechanical planarization (CMP) is one of the fabrication processes for electrode formation and barrier layer removal. The abrasive particles can be easily contaminated on the top surface during the CMP process. This can induce adverse effects on subsequent patterning and film deposition processes. In this study, a post Ru CMP cleaning solution was formulated by using sodium periodate as an etchant and citric acid to modify the zeta potential of alumina particles and Ru surfaces. Ru film (150 nm thickness) was deposited on tetraethylorthosilicate (TEOS) films by the atomic layer deposition method. Ru wafers were cut into $2.0{\times}2.0$ cm pieces for the surface analysis and used for estimating PRE. A laser zeta potential analyzer (LEZA-600, Otsuka Electronics Co., Japan) was used to obtain the zeta potentials of alumina particles and the Ru surface. A contact angle analyzer (Phoenix 300, SEO, Korea) was used to measure the contact angle of the Ru surface. The adhesion force between an alumina particle and Ru wafer surface was measured by an atomic force microscope (AFM, XE-100, Park Systems, Korea). In a solution with citric acid, the zeta potential of the alumina surface was changed to a negative value due to the adsorption of negative citrate ions. However, the hydrous Ru oxide, which has positive surface charge, could be formed on Ru surface in citric acid solution at pH 6 and 8. At pH 6 and 8, relatively low particle removal efficiency was observed in citric acid solution due to the attractive force between the Ru surface and particles. At pH 10, the lowest adhesion force and highest cleaning efficiency were measured due to the repulsive force between the contaminated alumina particle and the Ru surface. The highest PRE was achieved in citric acid solution with NaIO4 below 0.01 M at pH 10.
Journal of Institute of Control, Robotics and Systems
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v.5
no.5
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pp.568-576
/
1999
Using a laser sheet beam projector combined with a CCD-Camera, an efficient technique to recognize complex surface of curvature and lane has been demonstrated for the purpose of mobile robot navigation. In general, obstacles of indoor environments in the field of SLIT-RAY plane are captured as segments of an elliptical arc and a line in the camera image. The robot has been capable of moving along around the obstacle in front of it, by recognizing the original shape of each segment with the differential coefficient by means of least squares method. In this technique, the imaged pixels of each segment, particularly elliptical arc, have been converted into a corresponding circular arc in the real-world coordinates so as to make more feasible the image processing for the position and radius measurement than conventional way based on direct elliptical are analyses. Advantages over direct elliptical cases include 1) higher measurement accuracy and shorter processing time because the circular arc process can reduce the shape-specifying parameters, 2) no complicated factor such as the tilt of elliptical arc axis in the image plane, which produces the capability to find column position and radiua regardless of the camera location . These are essentially required for a mobile robot application. This technique yields an accuracy less than 2cm for a 28.5cm radius column located in the range of 70-250cm distance from the robot. The accuracy obtained in this study is sufficient enough to navigate a cleaning robot which operates in indoor environments.
Kim, Jin-Seok;Kim, Sung-Cho;Park, Jeong;Kim, Jeong-Soo
Journal of the Korean Society for Aeronautical & Space Sciences
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v.35
no.9
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pp.799-804
/
2007
The behavior of spray emanating from an injector to be employed in a liquid-propellant thrust chamber is investigated by optical measurement techniques. The injector has eight holes, each of which has 30 cant angle from the center-axis with the diameter of 0.406 mm. In order to examine an atomization process according to the spray-generation conditions and the evolution along spray downstream, variational features in the velocity and size of droplets obtained through Dual-mode Phase Doppler An 799emometry (DPDA) are delineated and discussed together with instantaneous plane images captured by using Nd:Yag laser sheet beam. A categorization of spray-flow regime representing the atomization and turbulent nature is made through evaluating the non-dimensional parameters, i.e., Reynolds number and Weber number based upon the theoretical injection velocity. These qualitative and quantitative data of spray breakup will be a firm basis for the design of brand-new thruster
Cu-added SKD11 was manufactured through the casting process and the effects of Cu addition with different contents (0, 1, 2 and 3 wt%) and aging treatment on microstructure, mechanical characteristics such as tensile strength and hardness, and thermal conductivity were investigated. The microstructure was analyzed by FE-SEM and XRD, the mechanical characteristics by Rockwell hardness tester and Tensile tester, and the thermal conductivity by Laser flash. As a result, SKD11 containing Cu had higher hardness than as-received SKD11. The hardness of as-cast SKD11 containing 1 wt% Cu was 42.4 HRC, whereas the hardness of asreceived SKD11 cast alloy was 19.5 HRC, indicating that the hardness was greatly improved when Cu was added. In the case of tensile strength, Cu-added SKD11 cast alloy had lower tensile strength than as-received SKD11, and the tensile strength tended to increase as Cu content increased. After heat treatment, however, tensile strength of as-received SKD11 was significantly increased, whereas in the case of Cu-added SKD11, as the Cu contents increased, the tensile strength increased less and even reduced at 3 wt% Cu. The thermal conductivity of Cu-added SKD11 cast alloy was about 13 W m-1 K-1, which was lower than that of the asreceived SKD11 cast alloy (28 W m-1 K-1). After the heat treatment, however, the thermal conductivity of as-received SKD11 was reduced, while the thermal conductivity of the SKD11 added with Cu was increased. Thermal conductivity was generally larger with less Cu content, and this tendency became more pronounced after heat treatment.
Proceedings of the Korean Society of Precision Engineering Conference
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2003.06a
/
pp.1869-1872
/
2003
As SLA(Sterealithography), SLS(Selective Laser Sintering), LOM(Laminated Object Manufacturing), FDM(Fused Deposition Modeling) etc. The FDM system the heart of a study and is developed by Stratasys co. ltd, in US., is small and cheap R.P. The material filament is heated until the material reaches a near-liquid state, it is pumped through a nozzle and become hand with a shape required, and this nozzle move pumping on the previously deposited material. Such FDM system that choice deposition type with X-Y plouter obtain in the thin continue layer by decreasing amount of extrusion or to central the injection amount when the head slow down at the corner, but in the process that fusion wax or resin become hand, deformation occur and it will affect the shape accuracy and the surface roughness. Such effect will depreciate quality and reliability of the product. Therefore, when the product made in actuality, the fundamental study on the basis geometry(surface, volume, line, angle) must be preceded and it have been research by many Free Form Fabrication. So, this basic object study purpose to obtain the fundamental geometry data and to enhance the surface roughness of the shape. And an operant can use the data for the progress of the surface roughness. This study research the estimation and application of the prototype surface roughness by adjustment the injection amount. And basie of this research, describe the pattern of prototype surface roughness and also used the result to estimate the surface of prototype.
Waste office paper, photocopied or laser printed, has recently increased along with office automatization. In waste office paper, toner ink is used as the printing medium in place of conventional oil ink. Since toner ink cannot be saponificated by alkali and be decolored by bleaching, different from the case of oil ink, toner remains on regenerated paper as black specks. Although cascade recycling of waste office paper is compelled at present, the demand for low-grade paper is limited. From such circumstances, a new separation process for waste office paper is demanded to achieve parallel recycling. At the first stage of separation studies, the sharpness of separation is evaluated using small separators to obtain fundamental data. In a lab-scale separator, the sample amount of one feed is generally a few grams. However, the sample amount used for brightness, ERIC, and image analysis that are generally used to evaluate the efficiency of deinking are not small for lab-scale tests of these analyses. This paper describes an investigation of a sheet preparation method by a small amount of sample under 0.5g and compares the precision of toner content determination of spectroscopic analysis and image analysis from the viewpoint of separation evaluation. The easiness and convenience of the operation using only general-purpose equipments has also been set as a principle purpose. From the viewpoint of an analysis that yields high precision with a small amount of sample in short time, the method calculating the absorption coefficient from diffuse reflectance in the visible region is the most rational method in this study.
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