• Title/Summary/Keyword: Large strains

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Cytogenetic and Histological Characteristics of Ginseng Hairy Root Transformed by Agrobacterium rhizogenes (Agrobacterium rhizogenes에 의하여 형질전환된 인삼 모상근의 세포 유전학적 및 조직학적 특성)

  • 고경민
    • Journal of Plant Biology
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    • v.36 no.1
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    • pp.75-81
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    • 1993
  • Ginseng (Panax ginseng C.A. Meyer) hairy root transformed with Agrobacterium rhizogenes (strain A4) was examined cytogenetically and histologically to assess its characteristics. The optimum growth of hairy root obtained in hormone-free MS medium (sucrose 30 g/L, pH5-6) for long period cultures. All hairy root strains (HB1, HB2, HB3) had the 2n diploid number (2n=48) of chromosomes in root tip cells. There were no alteration in chromosome structure except in one cell of HB3 strain. Results of SDS-PAGE showed a few difference in pattern and number of bands between normal and hairy root of ginseng. The root anatomy of normal root and hairy root differed each other. The hairy root had a clearly defined vascular strand, and the morphology of cortical cells were disorganised with large intercellular spaces.

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A New Record on Umbelopsis vinacea and Mucor hiemalis f. corticola Isolated from Korea

  • Das, Kallol;Ha, Ki-Hun;Suh, Sang-Jae;Lee, Seung-Yeol;Jung, Hee-Young
    • The Korean Journal of Mycology
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    • v.48 no.3
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    • pp.197-207
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    • 2020
  • In the screening of fungal diversity, two strains were collected from the soil of Yeongcheon and dissected guts from the bodies of Chinese rice grasshopper (Oxya chinensis), Chinese grasshopper (Acrida cinerea), and Far eastern devil grasshopper (Oedaleus infernalis) from Daejeon in Korea. They were identified as Umbelopsis vinacea (KNU-YC-1801B) and Mucor hiemalis f. corticola (KNU-20F7, KNU-20F8, KNU-20F9). Multigene phylogenetic analyses of the internal transcribed spacer (ITS) regions, and large subunit (LSU) sequence data confirmed two unreported taxa along with their morphology. The results of molecular phylogeny firmly supported the detailed description and illustration for each taxon. As far as we know, both Umbelopsis vinacea and Mucor hiemalis f. corticola are the first reported taxa in Korea.

Critical Current Properties of Bi-2223/Ag tapes with respect to axial Strain (Bi-2223/Ag 고온초전도 선재의 변형에 따른 임계전류 특성)

  • 하홍수;오상수;하동우;심기덕;김상철;배성우;권영길;류강식
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2000.07a
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    • pp.810-813
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    • 2000
  • In this study, we fabricated Bi-2223/Ag high temperature superconducting tapes using PIT (Powder-In-Tube) process to apply the superconducting magnet, cable and etc. It's inevitable to deform the superconducting tapes with axial strain for application. Therefore, for the characterization of the strain sensitivity of the superconducting properties, the degradation of Bi-2223/Ag tapes due to axial strain were investigated by measuring the critical current as a function of applied tension strain and external magnetic field. The critical current of Bi-2223/Ag tapes were decreased slightly up to 0.3∼0.4% applied strain but, drastically decreased more than these strains. Superconducting filament cores consisted of brittle ceramic fibers were broken easily by the large strain and current path were decreased simultaneously.

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A New Report on Three Species of Sordariomycetes Class Isolated from Soil in Korea

  • Das, Kallol;Lee, Seung-Yeol;Jung, Hee-Young
    • The Korean Journal of Mycology
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    • v.46 no.2
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    • pp.134-144
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    • 2018
  • Three fungal strains belonging to the class Sordariomycetes, namely BH-06, 17-039 and BE12-1, were isolated from soils in Korea and identified as Chaunopycnis alba, Myrothecium cinctum and Humicola olivacea, respectively. These species were confirmed according to their morphological characteristics and phylogenetic relationships determined based on internal transcribed spacer regions, as well as large subunit, small subunit, ${\beta}$-Tubulin and RNA polymerase II largest subunit sequences of ribosomal DNA. These three species are the first members of the Sordariomycetes reported in Korea.

Two Endophytic Diaporthe Species Isolated from the Leaves of Astragalus membranaceus in Korea

  • Kim, Jin-Hee;Kim, Dong-Yeo;Park, Hyeok;Eom, Ahn-Heum
    • Mycobiology
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    • v.45 no.4
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    • pp.430-433
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    • 2017
  • We characterized two endophyte fungi from the leaves of Astragalus membranaceus in Korea. The isolated strains were identified on the basis of the morphological characters and sequences analysis of the internal transcribed spacer and large subunit regions of the rDNA and ${\beta}$-tubulin gene. To the best of our knowledge, this is the first report of Diaporthe oncostoma and Diaporthe infecunda in Korea, and we have provided descriptions and figures.

A variable layering system for nonlinear analysis of reinforced concrete plane frames

  • Shuraim, Ahmed B.
    • Structural Engineering and Mechanics
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    • v.11 no.1
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    • pp.17-34
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    • 2001
  • An improved method has been developed for the computation of the section forces and stiffness in nonlinear finite element analysis of RC plane frames. The need for a new approach arises because the conventional technique may have a questionable level of efficiency if a large number of layers is specified and a questionable level of accuracy if a smaller number is used. The proposed technique is based on automatically dividing the section into zones of similar state of stress and tangent modulus and then numerically integrating within each zone to evaluate the sectional stiffness parameters and forces. In the new system, the size, number and location of the layers vary with the state of the strains in the cross section. The proposed method shows a significant improvement in time requirement and accuracy in comparison with the conventional layered approach. The computer program based on the new technique has been used successfully to predict the experimental load-deflection response of a RC frame and good agreement with test and other numerical results have been obtained.

Simulation of superelastic SMA helical springs

  • Mehrabi, Reza;Ravari, Mohammad Reza Karamooz
    • Smart Structures and Systems
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    • v.16 no.1
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    • pp.183-194
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    • 2015
  • Shape memory alloy (SMA) helical springs have found a large number of different applications in industries including biomedical devices and actuators. According to the application of SMA springs in different actuators, they are usually under tension and torsion loadings. The ability of SMAs in recovering inelastic strains is due to martensitic phase transformation between austenite and martensite phases. Stress or temperature induced martensite transformation induced of SMAs is a remarkable property which makes SMA springs more superior in comparison with traditional springs. The present paper deals with the simulation of SMA helical spring at room temperature. Three-dimensional phenomenological constitutive model is used to describe superelastic behavior of helical spring. This constitutive model is implemented as a user subroutine through ABAQUS STANDARD (UMAT), and the process of the implementation is presented. Numerical results show that the developed constitutive model provides an appropriate approach to captures the general behavior of SMA helical springs.

Analysis of thermo-rheologically complex structures with geometrical nonlinearity

  • Mahmoud, Fatin F.;El-Shafei, Ahmed G.;Attia, Mohamed A.
    • Structural Engineering and Mechanics
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    • v.47 no.1
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    • pp.27-44
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    • 2013
  • A finite element computational procedure for the accurate analysis of quasistatic thermorheological complex structures response is developed. The geometrical nonlinearity, arising from large displacements and rotations (but small strains), is accounted for by the total Lagrangian description of motion. The Schapery's nonlinear single-integral viscoelastic constitutive model is modified for a time-stress-temperature-dependent behavior. The nonlinear thermo-viscoelastic constitutive equations are incrementalized leading to a recursive relationship and thereby the resulting finite element equations necessitate data storage from the previous time step only, and not the entire deformation history. The Newton-Raphson iterative scheme is employed to obtain a converged solution for the non-linear finite element equations. The developed numerical model is verified with the previously published works and a good agreement with them is found. The applicability of the developed model is demonstrated by analyzing two examples with different thermal/mechanical loading histories.

Development of New Edible Pigments using Monascus spp.

  • Cho, Gyu-Seong;Kim, Kwangwook;Park, Won-Jong
    • The Korean Journal of Food And Nutrition
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    • v.32 no.1
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    • pp.41-49
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    • 2019
  • Carotene, xanthophyll, carotenoid anthocyan, phycopyrine, chlorophyll, and monascus pigments are used as natural coloring agents since they are more stable to human body than synthetic coloring agents. Among them, monascus pigments are a natural red pigment produced by the Monascus purpureus. For the development of edible paint using natural pigment, Monascus purpureus strain was cultured at a temperature of $35^{\circ}C$ for 15 days on a PDYA plate and liquid medium to produce a red pigment. In addition, a large amount of the red pigment was extracted from Hongkuk Koji in parallel with water extraction and ultrasonic wave extraction. At this time, the yield of ultrasonic extract was 2~4 times higher. Thus, Monascus purpureus strains, etc. were prepared by freeze-drying powder. In conclusion, natural paints made with red pigments have enabled the development of been edible paints that can be used as eco-friendly materials with good viscosity, enhanced spread ability and coloration.

Molecular and Morphological Confirmation of Three Undescribed Species of Mortierella from Korea

  • Nguyen, Thuong T.T.;Park, Se Won;Pangging, Monmi;Lee, Hyang Burm
    • Mycobiology
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    • v.47 no.1
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    • pp.31-39
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    • 2019
  • Three fungal isolates designated as CNUFC-YR329-1, CNUFC-PTS103-1, and CNUFC-PTS2-1 were discovered during a survey of fungal diversity of the order Mortierellales from freshwater and pine tree rhizosphere soil samples in Korea. The strains were analyzed morphologically and phylogenetically based on the internal transcribed spacer (ITS) and large subunit (LSU) of ribosomal DNA gene sequences. Based on their morphology and phylogeny, the three isolates were identified as Mortierella elongata, M. horticola, and M. humilis, respectively. To the best of our knowledge, M. elongata, M. horticola, and M. humilis, belonging to an undiscovered taxon, have not been previously described in Korea.