• Microbiology and Biotechnology Letters
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• v.24 no.2
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• pp.143-148
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• 1996

Thousand actinomycetes and 50 soil samples were used for the isolation of microorganisms producing yeast cell wall lytic enzymes. Among 493 strains producing large clear zones on autolysed washed yeast (AWY), 117 strains were selected on living yeast cell agar plates. With the method of lytic activity, one strain (St-1702) was selected, which was temporarily identified as Streptomyces eurythermus. The optimal condition for enzyme production of this strain was partially determined as follows: incubation of the strain for 3 days at 30$\circ$C in the medium containing 2% freeze dried yeast cell, 1% glucose, 1% K$_{2}$HPO$_{4}$, 0.01% MgSO$_{4}$'7H$_{2}$O, 0.5% peptone, and 0.2% (NH$_{4}$)$_{2}$CO$_{3}$ with pH 7.0. The protoplast formation of yeast by using the enzyme produced by this strain was compared with commercial enzymes.

• Transactions of Materials Processing
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• v.5 no.1
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• pp.18-26
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• 1996

In order to investigate the effect of pre-cooling of ingot on void closure in hot plate forging the internal strain and stress distributions are examined quantitatively by using ABAQUS. Simula-tions are carried out on a large slab ingot having the same temperature and the temperature gradient induced by air-cooling. It is shown that pre-cooling produces little effect on the strain behavior but remarkable effect on the hydrostatic stress at the central zone of ingot. The main factors for crushing micro-voids are the effective strain and the time integral of hydrostatic stress in the region surrounding the voids. Based on regression analysis it was found that the distortion of void can be expressed as a polynomial function of the two factors.

• KSBB Journal
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• v.9 no.4
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• pp.378-384
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• 1994

An improved method for the selective isolation of high-yielding mutant strains for the production of antifungal antibiotic KRF-001 was investigated. The mutant strain U. V 4, which produces high titer of KRF-001, was selected on the high potency agar plate after ultraviolet light irradiation. The U. V 4 strain produced 2-fold more KRF-001 than the mother strain in production media. Large scale fermentation was performed using the U. V 4 strain in 100$\ell$ fermenter. The antifungal antibiotic KRF-001 secreted into culture broth was detected by HPLC in 24hrs of fermentation.

• The Korean Journal of Mycology
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• v.45 no.1
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• pp.63-67
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• 2017

Through a survey of symbiotic endophytic fungi, we isolated an endophyte fungal strain from the roots of Glycyrrhiza uralensis in Korea. The isolated fungal strain was identified using its morphological characteristics and through phylogenetic analysis of the internal transcribed spacer, the large subunit rDNA region, and the translation elongation factor region. The strain was identified as Neocosmospora rubicola. This species has not been previously reported in Korea. In this study, we report its isolation from the roots of Glycyrrhiza uralensis in Korea, followed by the characterization and identification of the strain.

• Journal of Mechanical Science and Technology
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• v.19 no.7
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• pp.1460-1468
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• 2005

Two possible shape memory processes, austenite to detwinned martensite transformation and twinned martensite to detwinned martensite transformation of a shape memory alloy have been modeled and examined. Eshelby's equivalent inclusion method with Mori-Tanaka's mean field theory is used for modeling of the shape memory processes of TiNi shape memory alloy reinforced aluminum matrix composite. The shape memory amount of shape memory alloy, plastic strain and residual stress in the matrix are computed and compared for the two processes. It is shown that the shape memory amount shows differences in a small prestrain region, but the plastic strain and the residual stress in the matrix show differences in the whole prestrain region. The shape memory process with initially martensitic state of the shape memory alloy would be favorable to the increase in the yield stress of the composite owing to the large compressive residual stress and plastic strain in the matrix.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2005.11a
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• pp.727-730
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• 2005

Cellulose based Electro-Active Papers (EAPap) is very promising material due to its merits in terms of large bending deformation, low actuation voltage, ultra-lightweight, and biodegradability. These advantages make it possible to utilize applications, such as artificial muscles and achieving flapping wings, micro-insect robots and smart wall papers. This paper investigates the in-plane strains of EAPap under electric fields, which are useful for a contractile actuator application The preparation of EAPap samples and the in-plane strain measurement system are explained, and the test results are shown in terms of electric field, frequency and the oriental ions of the samples. The power consumption and the strain energy of EAPap samples are discussed. Although there are still unknown facts in EAPap material, this in-plane strain may be useful for artificial muscle applications.

• Geomechanics and Engineering
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• v.25 no.4
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• pp.341-345
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• 2021

1D sand compression response to ko-loading experiences volume contraction from low to high effective stress regimes. Previous study suggested compressibility model with physically correct asymptotic void ratios at low and high stress levels and examined only for both remolded clays and natural clays. This study extends the validity of Enhanced Terzaghi model for different sand types complied from 1D compression data. The model involved with four parameters can adequately fit 1D sand compression data for a wide stress range. The low stress obtained from fitting parameters helps to identify the initial fabric conditions. In addition, strong correlation between compressibility and the void ratio at low stress facilitates determination of self-consistent fitting parameters. The computed tangent constrained modulus can capture monotonic stiffening effect induced by an increase in effective stress. The magnitude of tangent stiffness during large strain test should not be associated with small strain stiffness values. The use of a single continuous function to capture 1D stress-strain sand response to ko-loading can improve numerical efficiency and systematically quantify the yield stress instead of ad hoc methods.

• Progress in Superconductivity and Cryogenics
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• v.12 no.3
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• pp.1-6
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• 2010

Since the fiber reinforced polymeric (FRP) composites are considered in next generation of space transportation systems, reliable thermal expansion properties should be well provided for structural design of composite materials. To obtain accurate mechanical behaviors at a cryogenic temperature, precise strain measurement and calibration must be provided. In this work, apparent strains (or thermal output) of temperature self-compensated strain gages were deliberately investigated for epoxy, CTBN modified epoxy and carbon fabric composite system from room temperature to liquid nitrogen temperature. Also, fourth-order thermal output curves were presented for the further calibration. The results showed that the thermal output is heavily dependent on test materials and a large amount of apparent strains were observed for the polymer resins.

• Microbiology and Biotechnology Letters
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• v.51 no.4
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• pp.551-554
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• 2023

We report the draft genome sequence of Sporobolomyces phaffii RJAF-17, a basidiomycetous yeast strain producing lipoamino acid surfactants, N-palmitoyl leucine and N-parmitoleyl glutamine. The annotation and classification of protein-coding genes provided the basic information for the genome of strain RJAF-17, including prediction of abundant genes as well as detection of genes involved in the biosynthesis of lipoamino acids. With the molecular importance of lipoamino acids as promising alternatives to chemical surfactants, the genomic information of strain RJAF-17 can help us understand the role of biomolecules in yeasts and explore possibilities of large-scale synthesis for industrial applications.

• Parasites, Hosts and Diseases
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• v.29 no.2
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• pp.149-160
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• 1991

Each of SPF mice(Scl: ICR strain, 3-week-old males) was inoculated with 5$\times$104 oocysts of Cryptosporidium by stomach tube. The oocysts were large type one which was previously isolated from Korean mice, and passaged in 3-week-old SPF mice. The patterns of oocyst discharge were monitored daily, and in order to observe the ultrastructure of developmental stages the stomach of the mice was examined by transmission electron microscopy (TEM) at 4 weeks post-inoculation. The prepatent period for 6 mice was 5.6 days post-inoculation on the average, and the patent period was 63.2 days. The number of oocysts discharged per day from the mice reached peak on day 36.6 post-inoculation on the average. A large number of oocysts were found in fecal samples obtained from inoculated mice on days 30~50 post-inoculation. C. tsuris was larger than C. parvum at almost every developmental stages, the sixte difference being 1.4 times in oocysts, 2.4 times in sporozoites, 1.6 times in merozoites, and 1.5 times in microgametes. The ultrastructural features of the attachment site of C. tsuris to the mucus cells were remarkably different from those of C. parvum and its closely related species. The anterior projection of the protozoa (C. muris), the outer aspect of which was surrounded by a thick filamentous process of the host cell, has not been reported at any developmental stages of C. parvum or its closely related species. The size of the oocysts of strain RN 66 was larger than that of Korean mice origin. The above results reveal that the large type Cryptosporidium of Korean mice origin is identified as Cryptosporidium muris and this type was named as C. muris (strain MCR).