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Analysis of the Foreign Gene Transmission in the GFP Transgenic Chickens (형질전환 닭에서 GFP 유전자 전이 연구)

  • Jang, Ye-Jin;Ji, Mi-Ran;Jeon, Mi-Hyang;Kim, Jeom-Sun;Kim, Kyung-Woon;Han, Deug-Woo;Chung, Hak-Jae;Yang, Byoung-Chul;Yoo, Jae-Gyu;Park, Jin-Ki;Kim, Te-Oan;Byun, Sung-June
    • Korean Journal of Poultry Science
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    • v.39 no.3
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    • pp.241-244
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    • 2012
  • This study was performed to analyze the generational transmission and the expression of the foreign gene in the GFP transgenic chickens. The transmission rate and the expression of the GFP gene was investigated from the GFP transgenic rooster (G2) as the first founder to the ninth (G8). Analysis of GFP expression in hatched chickens was used the UV lamp. When GFP was expressed in the wings, bill and legs of a chick, the bird only was selected as a transgenic chick. The average transmission rate of the overall transgenic was 38~58%. These results showed that the transmission of the GFP gene in the transgenic chickens in accordance with the laws of Mendel's continues to the next generation without gene silencing.

An Analysis of Fall Incidence Rate and Its Related Factors of Fall in Inpatients (입원환자 낙상 발생 실태와 원인에 관한 분석 연구)

  • Kim, Chul-Gyu;Suh, Moon-Ja
    • Quality Improvement in Health Care
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    • v.9 no.2
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    • pp.210-228
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    • 2002
  • Background: The purpose of this research was to examine the fall incidence rate and its related factors of fall in inpatients. Methods: The data were collected from the 138 fall incident reports in one tertiary hospital in Seoul from April 1st 1999 to September 30th 2001. The Fall Incident Report Form was originally developed based on that of Massachusetts General Hospital revised in 1995. And this was modified for this survey by the collaborating work of QI team including researcher and department of nursing service of this particular hospital. The contents of Fall Incident Form were general characteristics of patient. factors related to fall. types and places of fall. circumstances, nursing interventions. and outcome. Results: 1) The incidence rate of fall was 0.08% of total discharged patients and 0.081 per 1000 patient-day. This incidence rate is much lower than that of several hospitals in USA. This finding might result from the different incidence report system of each hospital. 2) The characteristics of fall-prone patient were found as follows. They were mostly over 60 years old, in alert mental status, ambulatory with some assistance, and dependent on ambulatory device. The types of diseases related high incidence rate were cerebrovascular disease(3.2), hypertension(1.6), cardiovascular disease(1.4), diabetes(1.3) and liver disease(0.6). 3) The majority of fall events usually occurred m bed. bedside(walking or standing) and bathroom in patient room. Usually they were up on their own when they fell. And there were more falls of elderly occurred during night time than day or evening. 4) 63.8% of fall events resulted in physical injuries such as fracture and usually the patients had diagnostic procedures and some treatment(ex. suture) which caused additional cost to the patients and their families. 5) The found risk factors of fall were drugs(antihypertensive drug, diuretics) and environmental factors like too high bed height, long distance of bedside table and lamp switch, and slippery tile of bathroom floor. Conclusion: Considering these results, every medical and nursing staff should be aware of the risk factors of patients in hospital, and should intervene more actively the preventive managements, specially for the elderly patients during night. Therefore, it is recommended that the development of Fall Prevention Programs based on these results.

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Relationship between the Catch of Squid, Todarodes pacificus STEENSTRUP, According to the Jigging Depth of Hooks and Underwater Illumination in Squid Jigging Boat (소형 오징어 채낚기 어선의 낚시 깊이별 조획량과 수중 조도)

  • CHOI Sok-Jin;ARAKAWA Hisayuki
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.34 no.6
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    • pp.624-631
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    • 2001
  • The relationship between the catch of squid, Todarodes pacificus, according to the jigging depth and underwater illumination by fishing lamps was investigated during nighttime operations off-Tusima Islands in November 1994 and off-Oki Islands in November 1995. We used echo sounder to observe the distribution of squid. Echo images on the echo sounder showed the distribution of squid at the water layer of 50 meter depth at the beginning of jigging operation. After the time elapsed, a continuous dense image had moved to the layer of 60-80 meters jigging depth. A larger number of squid were caught by jigging machines set at a lowest depth of 90 meters, when it compared with machines set at a 60 meters. However, Catch increased around 60 meters jigging depth, when fishing lamps output were switched to 24 kW halogen lights:.The underwater illumination, under the each light power of fishing lamps of squid jigging boat was continuously measured with an underwater illuminometer. Values of the underwater illumination, when schools were distributed from 60 to 80 meters, ranged from $3.0\times10^{-2}lx\;to\;3.4\times10^{-3}lx$ in average at $80\~360$ kW fishing lamps output of squid jigging boat.

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Microencapsulation of SrAl2O4 : Eu2+,Dy3+ Phosphorescent Phosphor for Enhanced Visibility of Road Lanes (차선의 시인성 향상을 위한 SrAl2O4 : Eu2+,Dy3+ 축광 마이크로 캡슐화에 관한 연구)

  • Park, Jae Il;Jeong, Soo Hwan;Cheong, In Woo
    • Journal of Adhesion and Interface
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    • v.17 no.3
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    • pp.110-116
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    • 2016
  • A decrease in the retro-reflectivity of glass-bead-covered road paint because of a rainwater film significantly reduces the visibility of drivers at night, and has been considered as a critical cause of traffic accidents. For enhanced visibility, the microencapsulation of hydrophobically modified $SrAl_2O_4:Eu^{2+}$,$Dy^{3+}$ phosphorescent phosphor was carried out via suspension polymerization of methyl methacrylate (MMA). The effects of surface modification agent and radical initiator types, loading amount of phosphorescent phosphor, and microcapsule size on the phosphor content ($W_{TGA}$) in the luminous poly(methyl methacrylate) (PMMA) microcapsules were investigated by thermogravimetric analyses (TGA). It was found that the $W_{TGA}$ value was ranged from 7 wt% to 81 wt%, which suggests suspension polymerization is suitable for the preparation of luminous microcapsules with a wide range of phosphor content. At a lower loading amount of phosphor, the $W_{TGA}$ value obviously increased as the microcapsule size decreased; however, the $W_{TGA}$ values with a higher loading amount of phosphor were less affected by the microcapsule size. The luminous microcapsules with the size range of $425{\sim}710{\mu}m$ were collected and tested as a luminous road lanes. It was found that luminance intensities of the microcapsule-coated plates remained higher than $300mcd/m^2$ for up to 100 s in darkness after 20 min of light emitting diode lamp irradiation. The results suggest that the luminous microcapsules can be a candidate for the replacement of glass beads for enhanced visibility of drivers.

Temperature dependency of the ZnO nanostructures grown by metalorganic chemical vapor deposition (MOCVD법으로 성장한 ZnO 나노구조의 온도 의존성)

  • Choi, Mi-Kyung;Kim, Dong-Chan;Kong, Bo-Hyun;Kim, Young-Yi;Ahn, Chel-Hyun;Han, Won-Suk;Mohanta, Sanjay Kumar;Cho, Hyung-Koun;Lee, Ju-Young;Lee, Jong-Hoon;Kim, Hong-Seung
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2008.06a
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    • pp.20-20
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    • 2008
  • 최근 LEDs가 동일 효율의 전구에 비해 에너지 절감 효과 크며 신뢰성이 뛰어나다기 때문에 기존 광원을 빠르게 대체해 나가고 있다. 특히 자외선 파장을 가지는 LEDs는 발열이 낮아 냉각장치가 필요 없으며, 수명이 길어 기존 UV lamp에 비해 많은 장점을 가지고 있기 때문에 많은 관심을 밭고 있다. 그럼에도 불구하고 자외선 LEDs는 제조 단가가 높고 power가 낮아 소요량이 많은 등 아직 해결해야 할 부분이 많기 때문에 이를 해결하기 위해 여러가지 재료와 다양한 구조가 고려되고 있다. 그 중 ZnO는 II-VI족 화합물 반도체로써 UV영역의 넓은 밴드갭(3.37eV)을 가지는 투명한 재료이다. 특히 ZnO는 60meV의 큰 엑시톤 결합에너지를 가지며, 가시광 영역에서 높은 투과율을 가지고, 상온에서 물리적, 화학적으로 안정하기 때문에 UV sensor, UV laser, UV converter, UV LEDs 등 광소자 분야에서 연구가 활발히 진행되고 있다. ZnO가 광소자의 발광재료로써 높은 효율을 얻기 위해서는 결정성을 높여 내부 결함을 감소시키며, 발광 면적을 높일 수 있는 구조가 요구된다. 특히 MOCVD 법으로 성장한 나노막대는 에피성장되어 높은 결정성을 기대할 수 있으며, 성장 조건을 조절함으로써 나노막대의 aspect ratio와 밀도 제어할 수 있기 때문에 표면적을 효과적으로 넓혀 높은 발광효율을 얻을 수 있다. 본 실험에서는 MOCVD 법으로 실리콘과 사파이어 기판 위에 다양한 성장 온도를 가진 나노구조를 성장 시키고 온도에 따른 형상 변화와 특성을 평가하였다. ZnO 의 성장온도가 약 $360^{\circ}C$ 일 때, 밀도가 조밀하고 기판에 수직 배열한 균일한 나노막대가 성장되었으며 우수한 결정성, 광학적 특성이 나타남을 SEM, TEM, PL, XRD를 사용하여 확인하였다.

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Research a Person's Eyesight Changes on According to the Optimum Color Temperature for the Stand Lamp Using White Light LED Sources (백색광 LED를 사용한 독서등의 최적 색온도에 따른 사람의 시력 변화 연구)

  • Kim, Juhyun;Chang, Wonbeom;Lee, Seokhwan;Jung, Kwangkyo;Kim, Donghyun;Kim, Jeongmi;Ryu, Jaejun;Moon, Seongdeuk;Lee, Seunghyun;Ko, Youngsu;Huh, San;Jang, Mina;Jung, Changho;Chang, Jiho
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.26 no.1
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    • pp.80-82
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    • 2013
  • White light emitting diode (LED) determined the most appropriate color temperature in reading lighting evaluated fatigue degree of eye according to color temperature. The eye fatigue degrees are determined by brightness and color temperature. Therefore, we measured the results of eyes test according to the change of color temperature and brightness. Experiments except for astigmatic corrected visual acuity of 0.8 more and age 20 to 25 years old, male and female college students was conducted in 100 patients. And constant illumination conditions, visual acuity was measured by varying the color temperature. The optometry at 10 minutes in the darkroom adapted eye. And then the temperature of $25{\pm}3$ degrees, the humidity was carried out at $50{\pm}5%$. As a result of typical color temperature of white light (5,600 K) has identification of the readability.

Optimization of Optical Structure of Lightguide Panel for Uniformity Improvement of Edge-lit Backlight (엣지형 LED 백라이트의 균일도 향상을 위한 도광판의 광구조 최적화)

  • Lee, Jung-Ho;Nahm, Kie-Bong;Ko, Jae-Hyeon;Kim, Joong-Hyun
    • Korean Journal of Optics and Photonics
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    • v.21 no.2
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    • pp.61-68
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    • 2010
  • Optical simulation methods were applied to the edge-lit LED backlight for LCD TV applications in order to optimize the optical structure of the light guide plate(LGP), and thus to improve the uniformity properties by removing the bright spots caused by LED's. The edge-lit LED backlight consisted of three white LED's with a lamp cover, a light guide plate, and a reflection film. When there was no pattern on the entrance side surface of the LGP, the illuminance uniformity was sensitively dependent on the distance d between the LED and the entrance surface. The illuminance uniformity increased with d but its increasing rate slowed down when d was beyond ~ 1.5 mm. When micro-patterns such as a lenticular lens array (LLA) or a serration pattern were formed on the entrance surface, the illuminance uniformity was improved substantially even for the case of very small d. At the same simulation condition, the lightguide with serration pattern showed a better uniformity than that with LLA pattern. Additional improvement could be achieved by changing the refractive index of the micro-patterns. These results suggest that using micro-patterns is a very effective way to reduce the bright spots due to their refracting function for the concentrated incident rays onto the LGP.

Changes in Allicin Contents of Garlic via Light Irradiation (광 조사에 의한 마늘의 알리신 함량 변화)

  • Jeong, Hoon;Lee, Sun-Ho;Yun, Hong-Sun;Choi, Seung-Ryul
    • Food Science and Preservation
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    • v.20 no.1
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    • pp.81-87
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    • 2013
  • Agri-food garlic has been recognized as healthful because of its antibacterial, anticancer and antioxidant effects. As such, its consumption is steadily increasing. This study was conducted to amplify the healthful ingredient of garlic, allicin, using light irradiation. The following conclusions were drawn from the investigation of peeled garlic under various conditions like fluorescent lighting, color (green, yellow, blue and red) and ultraviolet light (UV-A, UV-B and UV-C). The allicin content increased most with the 15-second $0.129W/m^2$ (40 W) UV-B treatment of the garlic at a 700 mm distance from the light source. At the treatment of the garlic with 126 lx (40W) red light for 24 hours at a 700 mm distance from the light source, its allicin content increased from $15.15{\pm}0.25mg/g$ to $15.15{\pm}0.34mg/g$, for a 10-percent amplification effect. Therefore, it is believed that the healthfulness of garlic can be amplified through irradiation processing of its healthful ingredient, allicin, and the development of its processing unit.

Automatic Skin Basal Cell Carcinoma Detection Using Protophorphyrin IX((PpIX) Fluorescence Image (PpIX 형광영상을 이용한 피부 기저세포암 자동검출)

  • Yu, Hong-Yeon;Jun, Do-Young;Kim, Min-Sung;Hong, Sung-Hoon
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.45 no.1
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    • pp.47-54
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    • 2008
  • In this paper, we propose an auto-detection algorithm of basal cell carcinoma(BCC) from the protophorphyrin IX(PpIX) fluorescence image induced by appling the methyl 5-aminolaevulinate(MAL) ointment-induced protophorphyrin IX(PpIX) to the skin tumour area and then shining the wood lamp on the area. The proposed algorithm first generates 3 mask areas-tumor area, suspected tumor area and tumor free area and then applies local watershed algorithm to the turner and the suspected tumor areas to make small watershed regions that include similar luminance value pixels. Next, small watershed regions are merged by hierarchical queue based fast region merging that uses the difference between the average luminance values of adjacent watershed regions as a region merging criterion and finally BCC regions are detected. 50 tissue samples are acquired from the tumour regions of 10 patients with BCC that are extracted by using the proposed algorithm and are performed pathological examination by expert dermatologist. Experiment result shows the rate of tumor detection from BCC lesion using presurgical in vivo of MAL-indeuced PpIX fluorescence has high sensitivity 94.1% and relatively high specificity 82.6%.

Crystal Growth of rutiles doped with Impurity Ions by Floating Zone Method (부유대용융법에 의한 불순이온 주입된 $TiO_2$단결정 성장 연구)

  • 이성영;유영문;김병호
    • Proceedings of the Korea Crystallographic Association Conference
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    • 1999.04a
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    • pp.1-1
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    • 1999
  • 부유대용융법에 의하여 불순이온의 종류와 각 이온의 주입 농도를 달리하는 Rutile 단결정을 성장하였다. 성장된 결정으로부터 제조한 박편시료를 이용하여 결정결함과 광투과도에 미치는 각 불순이온의 영향을 조사하였다. 결정성장용 주원료로 99.99%의 TiO2를 사용하고, 불순이온 주입을 위한 원료로서 99.99%의 Al2O3, H3BO3, Ga2O3, Sc2O3, V2O5, Fe2O3, ZrO2, Er2O3, Cr2O3를 각각 사용하였다. 불순이온의 종류에 따르는 영향을 조사하기 위하여 TiO2 99.8 atomic%-불순이온 0.2atomic%의 조성이 되도록 각 이온별로 원료를 정밀하게 평량하고 균일 혼합하였다. 불순 이온의 첨가량에 따르는 영향을 조사하기 위하여 Al2O3는 각각 pure, 0.2, 0.4, 0.6 atomic%를, Cr2O3는 pure, 0.003, 0.05, 0.2 atomic%를 각각 치환하여 원료를 조합하였다. 균일 혼합된 원료를 직경 8mm의 고무 튜브에 넣고 CIP(Cold Isostatic Press0에서 2000kg/$\textrm{cm}^2$의 압력으로 성형한 후 150$0^{\circ}C$에서 1시간 소결함으로서 결정성장용 다결정 원료를 합성하였다. 합성된 다결정을 double ellipsoidal mirror 내에 설치하고,halogen lamp로 가열하여 원료의 한쪽 끝을 용융한 다음, 20rpm의 회전속도, 3-5mm/hr의 성장속도로 하는 유속 1$\ell$/min의 O2 분위기속에서 부유대용융법에 의하여 결정을 성장하였다. 성장된 결정을 성장축에 수직한 방향으로 각각 절단, 연삭, 연마한 박편을 이용하여 편광하에서 low-angle grain boundaries 및 기타의 결정결함을 관찰하였으며, 0.3$\mu\textrm{m}$~0.8$\mu\textrm{m}$ 범위 및 0.6$\mu\textrm{m}$~3.4$\mu\textrm{m}$ 범위에서의 투과 및 흡수 스펙트럼을 측정하였다. 결정 성장 결과 B3+, Er3+, Cr3+ 이온은 Ti4+ 이온과 이온의 크기 차이가 심하여 결정의 정상적인 성장을 방해하는 물성을 나타냈고, V5+, Cr3+ 이온은 흑색의 결정, Fe3+ 이온은 적갈색의 결정으로 성장되었다. Al3+, Zr4+, Al3+의 순서로 투과도가 높아지는 것이 관찰되었다. 불순이온의 농도에 따른 영향으로서 Al3+ 이온의 경우 주입농도가 높아질수록 low angle boundary와 oxygen deficiency가 감소하였고, 투과율은 조금 감소하거나 큰 차이가 없는 것으로 나타났다. 반면에 Cr3+ 이온을 주입한 경우 0.003 atomic%에서 최적의 물성을 보였으며, 주입농도가 높아질수록 결정성장이 어려워지고 광의 투과도가 급격히 저하되었다.

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