• Journal of Plant Biotechnology
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• v.35 no.2
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• pp.115-120
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• 2008

L-Ascorbic acid (vitamin C) in vegetables is an essential component of human nutrition. The objective is to transform lettuce (Lactuca sativa L.) with GalUR gene that is involved in the vitamin C biosynthesis. The cotyledons of Hwoahong (Nongwoo Bio Co.) were used to induce the callus and shoot under the selection media with MS + 30 g/L Sucrose + 0.5 mg/L BAP + 0.1 mg/L NAA + 100 mg/L kanamycin + 200 mg/L lilacillin, pH 5.2. The shoot was developed from the cut side of the explants after 3 weeks on the selection media. We successfully transformed the lettuce with GaIUR gene and analyzed the levels of vitamin C. We found that some of the lettuce transgenic lines contained higher levels of vitamin C compared with the normal one (non-transformed). Especially, some of $T_1$ lettuces inserted by GalUR showed about $3{\sim}4$ times higher content of vitamin C compared to the non-transformed lettuce. This data support the previously work performed with GLOase transgenic $T_1$ lettuces from which several times higher content of vitamin C were identified. The $T_2$ lettuces with high content of vitamin C have been selected for further analysis.

• Journal of Life Science
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• v.14 no.1
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• pp.61-66
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• 2004

Many studies have been widely carried out to find out new compound having anti-cancer activity from animals and plants. Some plants have been reported to have anti-lancer effects. However, the anti-cancer effect of edible plants were seldomly evaluated. Therefore we investigated the anticancer effects of edible plants (10 samples) easily available around us by measuring number of survival cancer cells after treatment with direct cell counting and MTT analysis, and by examining the morphological change under the electromicroscope. Of the 10 samples tested, Equisetum arvense L., Lactuca dentata Mokino. var. faviflora Makino. showed moderate anti-cancer effects even at the concentration of 10 $\mul/ml$ against MCF-7 adenocarcinoma cell line. Of them, Capsicum annuum L. had most potent anti-cancer activity against MCF-7 adenocarcinoma cell line showing proliferation inhibited, morphological change and apoptosis at the concentration of 2 $\mul/ml$.

• KSBB Journal
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• v.22 no.2
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• pp.73-77
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• 2007

The effects of Fucoidan extracted from Ulva lactuca on carbon tetrachloride ($CCI_4$)-induced dysfunction in $CCI_4$-posttreated rats were investigated. Ulva lactuca fucoidan (ULF) of 100 mg/kg concentration was intraperitoneally administered into rats at dose of 1 ml/kg for 14 days. On the day 15, 3.3 ml/kg of $CCI_4$ dissolved in olive oil (1 : 1) was injected 12 hours before anesthetization. We examined the levels of glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT) in serum of rats, superoxide dismutase (SOD) in mitochondrial fraction and catalase (CAT), glutathione peroxidase (GPx), malondialdehyde (MDA) in liver of rats. SOD, CAT, GPx decreased, and GOT, GPT, MDA increased in the $CCI_4$-treated group. But SOD, CAT, GPx increased, and GOP, GPT, MDA decreased in the ULF and $CCI_4$-treated group. These results showed that ULF had the protective effects on the liver dysfunction of $CCI_4$-treated rats.

• Journal of Life Science
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• v.12 no.3
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• pp.340-348
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• 2002

Equisetum arvense L. and Lactuca dentata Makino. var. Flaviflora Makino. of samples relatively showed anticancer effects on MCF-7 mammary gland adenocarcinoma cell. The most active plant among the samples was Capsicum annuum L. var angulosum Mill. We studied that MCF-7 cells were changing chromosomal morphology and apoptosis on these samples. Capsicum annuum L. var. angulosum Mill. of samples relatively showed good anticancer effects. The cells became vague after 2 days and then destroyed. The supernatant of the cells including medium was measured by UV absorbance. The results showed that Capsicum annuum L. var. angulosum Mill also exerted high level. We also used electrophoresis in order to observe apoptic characterization of DNA fragmentation. The cells treated with Capsicum annuum L. var. angulosum Mill showed the apoptotic characterization. The chromosome of the cells were observed on those samples. The cells treated with Capsicum annuum L. var. angulosum Mill among them were shown the fastest changes. The cells were aggregated and destroyed by treatment with some edible plants. Especially, the case of Capsicum annuum L. var. angulosum Mill, it led MCF-7 cell to apoptosis faster than others. And we can observe chromosomal changes and dispersion by PI staining. These results showed that each sample exerted anticancer effects on MCF-7 cells. Especially Capsicum annuum L. var. angulosum Miff exerted significant anticancer effects.

• Proceedings of the Korean Society of Crop Science Conference
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• 2000.04a
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• pp.420-421
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• 2000

• Proceedings of the Korean Society of Life Science Conference
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• 2005.09a
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• pp.54-54
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• 2005

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2003.10a
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• pp.97-97
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• 2003

• Proceedings of the Korean Society of Life Science Conference
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• 2006.09a
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• pp.62.2-62.2
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• 2006

• Korean Journal of Plant Resources
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• v.33 no.4
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• pp.418-418
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• 2020

• Korean Journal of Agricultural Science
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• v.39 no.1
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• pp.111-116
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• 2012

Nondestructive evaluation of seed viability is one of the highly demanding technologies for seed production industry. Conventional seed sorting technologies, such as tetrazolium and standard germination test are destructive, time consuming, and labor intensive methods. Near infrared spectroscopy technique has shown good potential for nondestructive quality measurements for food and agricultural products. In this study, FT-NIR spectroscopy was used to classify normal and artificially aged lettuce seeds. The spectra with the range of 1100~2500 nm were scanned for lettuce seeds and analyzed using the principal component analysis(PCA) method. To classify viable seeds from nonviable seeds, a calibration modeling set was developed with a partial least square(PLS) method. The calibration model developed from PLS resulted in 98% classification accuracy with the Savitzky-Golay $1^{st}$ derivative preprocessing method. The prediction accuracy for the test data set was 93% with the MSC(Multiplicative Scatter Correction) preprocessing method. The results show that FT-NIR has good potential for discriminating non-viable lettuce seeds from viable ones.