• Title/Summary/Keyword: Lactuca

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Overexpression of Arabidopsis Homogentisate Phytyltransferase or Tocopherol Cyclase Elevates Vitamin E Content by Increasing γ-tocopherol Level in Lettuce (Lactuca sativa L.)

  • Lee, Koeun;Lee, Sa Mi;Park, Sang-Ryoung;Jung, Jinwook;Moon, Joon-Kwan;Cheong, Jong-Joo;Kim, Minkyun
    • Molecules and Cells
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    • v.24 no.2
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    • pp.301-306
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    • 2007
  • Tocopherols, essential components of the human diet, are synthesized exclusively by photosynthetic organisms. To increase tocopherol content by increasing total flux to the tocopherol biosynthetic pathway, genes encoding Arabidopsis homogentisate phytyltransferase (HPT/V-TE2) and tocopherol cyclase (TC/VTE1) were constitutively overexpressed in lettuce (Lactuca sativa L.). Total tocopherol content of the transgenic plants overexpressing either of the genes was increased by more than 2-fold mainly due to an increase in ${\gamma}$-tocopherol. However, chlorophyll content in the HPT/VTE2 and TC/VTE1 transgenic lines decreased by up to 20% and increased by up to 35%, respectively (P < 0.01). These results demonstrate that manipulation of the tocopherol biosynthetic pathway can increase or decrease chlorophyll content depending on the gene introduced.

Plant Growth-Promoting Rhizobacteria Improved Salinity Tolerance of Lactuca sativa and Raphanus sativus

  • Hussein, Khalid Abdallah;Joo, Jin Ho
    • Journal of Microbiology and Biotechnology
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    • v.28 no.6
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    • pp.938-945
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    • 2018
  • Salinity stress is an important environmental problem that adversely affects crop production by reducing plant growth. The impacts of rhizobacterial strains to alleviate salinity stress on the germination of Lactuca sativa and Raphanus sativus seeds were assessed using different concentrations of NaCl. Plant growth-promoting rhizobacteria (PGPR) strains were also examined to improve the early germination of Chinese cabbage seeds under normal conditions. Lactobacillus sp. and P. putida inoculation showed higher radicle lengths compared with non-inoculated radish (Raphanus sativus) seeds. LAP mix inoculation increased the radicle length of lettuce (Lactuca sativa) seedlings by 2.0 and 0.5 cm at salinity stress of 50 and 100 mM NaCl concentration, respectively. Inoculation by Azotobacter chroococcum significantly increased the plumule and radicle lengths of germinated seeds compared with non-inoculated control. A. chroococcum increased the radicle length relative to the uninoculated seeds by 4.0, 1.0, and 1.5 cm at 50, 100, and 150 mM NaCl concentration, respectively. LAP mix inoculation significantly improved the radicle length in germinated radish seeds by 7.5, 1.3, 1.2, and 0.6 cm under salinity stress of 50, 100, 150, and 200 mM NaCl concentration, respectively. These results of this study showed that PGPR could be helpful to mitigate the salinity stress of different plants at the time of germination.

Detection of Ecosystem Distribution Plants using Drone Hyperspectral Spectrum and Spectral Angle Mapper (드론 초분광 스펙트럼과 분광각매퍼를 적용한 생태계교란식물 탐지)

  • Kim, Yong-Suk
    • Journal of Environmental Science International
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    • v.30 no.2
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    • pp.173-184
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    • 2021
  • Ecological disturbance plants distributed throughout the country are causing a lot of damage to us directly or indirectly in terms of ecology, economy and health. These plants are not easy to manage and remove because they have a strong fertility, and it is very difficult to express them quantitatively. In this study, drone hyperspectral sensor data and Field spectroradiometer were acquired around the experimental area. In order to secure the quality accuracy of the drone hyperspectral image, GPS survey was performed, and a location accuracy of about 17cm was secured. Spectroscopic libraries were constructed for 7 kinds of plants in the experimental area using a Field spectroradiometer, and drone hyperspectral sensors were acquired in August and October, respectively. Spectral data for each plant were calculated from the acquired hyperspectral data, and spectral angles of 0.08 to 0.36 were derived. In most cases, good values of less than 0.5 were obtained, and Ambrosia trifida and Lactuca scariola, which are common in the experimental area, were extracted. As a result, it was found that about 29.6% of Ambrosia trifida and 31.5% of Lactuca scariola spread in October than in August. In the future, it is expected that better results can be obtained for the detection of ecosystem distribution plants if standardized indicators are calculated by constructing a precise spectral angle standard library based on more data.

Light dependent arsenic uptake and growth in Lactuca sativa L.

  • Hyun-Gi Min;Eunjee Kim;Min-Suk Kim;Jeong-Gyu Kim
    • Korean Journal of Environmental Biology
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    • v.41 no.4
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    • pp.697-705
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    • 2023
  • Along with other heavy metals, arsenic (As) is one among the substances most harmful to living organisms including humans. Owing to its morphological similarity to phosphorus, the uptake of As is influenced by photosynthesis and the phosphorus uptake pathway. In this study, we varied arsenic exposure and light intensity during nutrient solution cultivation of lettuce (Lactuca sativa L.) to determine the effect of these two factors on arsenic uptake, lettuce growth, and electron transfer in photosystem II. In the treatment exposed to 30 μmol L-1 of arsenic, the shoot arsenic concentration increased from 4.73 mg kg-1 to 18.97 mg kg-1 as the light intensity increased from 22 to 122 μmol m-2 s-1. The water content and ET2o/RC of the shoots were not affected by arsenic at low light intensity; however, at optimal light intensity, they decreased progressively with arsenic exposure. Increased light intensity stimulated the growth of plant roots and shoots; contrarily, the difference in growth decreased as the concentration of As exposure increased. The results of this study suggest that the effect of As on plant growth is dependent on light intensity; in particular, an increase in light intensity can increase the uptake of As, thereby affecting plant growth and As toxicity.

Changes of Soil Chemical Properties and Uptake of Salts by the Plants according to the Application of the Food Waste Compost (음식물찌꺼기 퇴비의 시용에 따른 토양의 화학성 변화 밑 작물체내 염류의 흡수)

  • Lee, Sang-Suk;Chang, Ki-Woon
    • Journal of the Korea Organic Resources Recycling Association
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    • v.6 no.2
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    • pp.59-67
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    • 1998
  • This study was conducted to investigate the changes of soil chemical properties and uptake of salts by the plants(Raphanus and Lactuca) according to the application of the food waste compost(FWC), which was produced on a large scale at a pilot plant. Generally, FWC has a high electrical conductivity(EC) and contains much salts, such as Na, K, Ca, and Mg, and because of these when FWC are applied to soils there is a possibility of salt ac-cumulation in soils and growth inhibition on plants. The FWC were applied at the rates of 0, 20, 40, 80, and 160 Mg/ha in the 1/5,000a pots on the basis of dry weight, and the plants were cultivated for 60 days. And then, changes of pH and EC in soils and uptake of salts by the plants were investigated. The results obtained are summarized as follows; 1. After the cultivation of the Raphanus and Lactuca, pH increased and EC decreased in the soils. 2. Uptake rates of Na and K were slightly increased with the application of the FWC. In uptake rate of Ca, Raphanus and Lactuca was decreased, increased, respectively. In Raphanus, uptake rate of Mg was highest with the application of the FWC at 40 Mg/ha, and in Lactuca, continuously increased with application of the FWC.

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Comparison of Post-Harvest Character and Storability at Several Temperature for Lactuca indica L. Baby and Adult Leaves (왕고들빼기 어린잎과 성체의 수확 후 특성과 온도별 저장성 비교)

  • Kim, Ju Young;Han, Su Jeong;Wang, Lixia;Choi, In-Lee;Kang, Ho-Min
    • Journal of Bio-Environment Control
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    • v.28 no.2
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    • pp.172-177
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    • 2019
  • Lactuca indica L. ('Seonhyang') baby leaves were harvested after cultivation for 4 weeks (less than 10 cm plant height) and adults were cultivated for 8 weeks ($20{\pm}5cm$ plant height). The respiration rate and the ethylene production rate of Lactuca indica leaves were higher than those of the baby leaves but the DPPH radical scavenging ability was lower than baby leaves. The $L^*$, $a^*$ and $b^*$ values did not show any significant difference between baby leaves and adult leaves but the chlorophyll content was higher in adult leaves. All adult and baby leaves of Lactuca indica were stored at $2^{\circ}C$, $8^{\circ}C$, and $20^{\circ}C$, respectively. The higher the storage temperature, the higher the fresh weight loss rate and weight loss rate of adult leaves was lower at $2^{\circ}C$ and $8^{\circ}C$. The visual quality of Lactuca indica leaves were determined by the panel test during storage and it deteriorated faster as the storage temperature increased. The shelf life that calculated the period of maintaining higher than 3 points of visual quality was longer than 1.6 days at $2^{\circ}C$, 1.4 days at $8^{\circ}C$ and 1.5 days at $20^{\circ}C$. The oxygen and carbon dioxide concentrations within the package of Lactuca indica leaves were similar to those in atmosphere. The chlorophyll content was maintained higher at lower storage temperature in the last storage day and the off-odor was higher in baby leaves than in the adult leaves of Lactuca indica L.

The Liver Protective Activities of Some Iranian Medicinal Plants Against Liver Damage in Mice Induced by $CCL_4$ Intoxication

  • Kalantari. H.;Aghel. N.;Annafecheh. M.;Mar, Woong-Chon;Chang, Il-Moo
    • Toxicological Research
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    • v.11 no.2
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    • pp.309-313
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    • 1995
  • The aim and objective of this study are to carry out the liver protective activities against the $CCl_4$ intoxication in mice with some Iranian medicinal plants traditionally used for liver injuries. The methanol extracts of Cichorium intybus, Lactuca scarJoia, Eucalyptus camadulensis were evaluated. With various doses of these plants, liver protective activities were performed after $CCl_4$ administration to mice. The serum aminotransferases activites, liver sizes, and histopatological examinations of liver were studied. At a dose of 50 mg/ kg, all three plants were able to protect liver damages induced by $CCl_4$.

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Dopamine ${\beta}-Hydroxylase$ Inhibitory Activity of Medicinal Plants (식물성 생약의 도파민 베타 수산화효소에 대한 저해활성)

  • Tae, Dong-Nyen;Hwang, Keum-Hee;Han, Yong-Nam
    • Korean Journal of Pharmacognosy
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    • v.26 no.1
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    • pp.62-65
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    • 1995
  • Dopamine ${\beta}-hydroxylase$ (DBH) catalyses the enzymatic reaction of dopamine to norepinephrine. For the purpose of isolating DBH inhibitors from natural resources, thirty one kinds of medicinal plants were screened by tracing the inhibitory activities against bovine adrenal DBH, utilizing tyramine as a substrate. Among the crude drugs tested, leaves of Lactuca sativa L., Gardeniae Fructus, Magnoliae Flos and Scutellariae Radix showed potent enzyme inhibitory activities against DBH.

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First Report of Sclerotinia Rot Caused by Sclerotinia sclerotiorum on Some Vegetable Crops in Korea

  • Chang, Seog-Won;Kim, Sung-Kee
    • The Plant Pathology Journal
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    • v.19 no.2
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    • pp.79-84
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    • 2003
  • Sclerotinia rot occurred severely on some vegetable crops grown in Namyangju, Yangpyung, and Yangiu areas in Korea in 2001-2002. The crops infected with Scterotinia sp. were Adenophora remotiflora, Armoracia lapathfolia, Angelica acutiloba, Angelica archangelica, Anthriscus sylvestris, Aster tataricus, Beta vulgaris var. cicla, Brassica campestris var. marinosa, Brassica juncea var. laciniata, Chicholium intybus, Lactuca indica var. dracoglossa, Lactuca sativa var. oak-leaf, Petroselinum crispum, and Phyteuma japonicum. The fungus associated with the disease was identified as Sclerotinia sclerotiorum, based on the morphological characteristics of the pathogen. The symptoms were water-soaked spots that enlarged later and became a watery soft rot. Infected parts became yellow and then turned brown, followed by death of the whole plant. White mycelia developed on the upper petioles and leaves and on the soil where these plant parts lay. Then black sclerotia in variable size and shape formed from the mycelial mass. Pathogenicity of the fungus was proven by artificially inoculating each crop. This is the first report of Sclerotinia rot on the listed vegetable crops in Korea.