• 한국산림과학회지
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• 제37권1호
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• pp.61-63
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• 1978

잣나무털녹병 (Korean white pine blister rust) 이병목(罹病木)의 해부학적(解剖學的) 진단(診斷)을 위(爲)하여, 이병부(罹病部) 수피(樹皮) 혹(或)은 목질부(木質部)의 면도날 절편(切片)을 Chloroform 1 : methanol 2 의 혼합액(混合液)에 24시간(時間) 침지(浸漬)하고, lactophenol cotton blue를 modify 한 액(液)(lactic acid 20gm, phenol crystal 20gm, cotton blue 0.05gm, 60% EtOH 100ml)에 2~5분(分) 염색(染色)하고 60~70% EtOH에 2~3분(分) 씻은 다음 lactophenol로 mount하여 검경(檢鏡)하였다. 기주조직중(寄主組織中)에 분기만연(分技蔓延)한 본병원균(本病原菌) Cronartium ribicola의 비교적(比較的) 넓은 균사(菌絲)와 곤봉상(棍棒狀) 혹(或)은 만곡(彎曲)한 흡기(吸器)가 청염(靑染)됨으로서, 잣나무의 세포조직(細胞組織)과 용이(容易)하게 식별(識別)할 수 있었다. 수피부(樹皮部)에서는 균사(菌絲) 발달(發達)이 양호(良好)하나 목질부(木質部)에서는 방사조치(放射組織)의 투심종단면(透心縱斷面)에서의 관찰(觀察)이 용역(容易)하였고 타(他) 목질부(木質部) 조직(組織)에서는 거의 관찰(觀察)할 수 없었다.

• 한국동물위생학회지
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• 제30권2호
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• pp.283-286
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• 2007

Fungus generally doesn't produce toxic or harmful substances so it has low chances to cause food poisoning. However it leads to change appearance, odor and characteristics of the contaminated foods and result in sanitary risk problems. Therefore the contamination of fungi should be prevented since they are not proper for human consumption. Green fungi with white outline raised from the air cell of cooked eggs which were collected by Gyeongi Livestock Veterinary Service in August, 2006. The results came out after the cultivation using Sabouraud's Dextrose Agar(SDA). The conidium appeared white and monospore, the shape of colony was round and oval. Conidiophore was brown and granulated and wrinkles and formed. It was confirmed as Aspergillus nidulans based on the dying using Lactophenol cotton blue, the observation of septum and vesicle from the grown spores, and rDNA sequencing.

• 한국동물위생학회지
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• 제23권1호
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• pp.71-76
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• 2000

The dermatophytes was isolated from skin of the 175 healthy dogs and 22 dogs with pathological skin lesions at Pohang and Kyongju. The isolates were identified by the morphological appearance after cultivation and lactophenol cotton blue staining. 1, The isolation rates of dermatophytes were 8.5%(15/175) in dog with healthy skin and 27% (6/22) in dogs with pathological skin lesions. 2. From asymptomatic dogs, the isolation rates of dermatophytes in female dogs were higher than those in male dogs and those in young dogs were higher than those in old dogs. 3. Isolation rates of microsporum canis and trichophyton spp from asymtomatic dogs were 14 (93%) and 1(6.7%), respectively. 4. The causative agents of 6 dogs with fermatophytosis were identified as all M canis.

• 한국산업보건학회지
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• 제20권4호
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• pp.256-262
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• 2010

This study evaluated the airborne concentrations of fungi in university laboratories, hospital diagnostic laboratories in Seoul. The incubated fungi was identified by lactophenol cotton blue (LPCB) staining method. Variables such as types of ventilation, temperature and relative humidity were investigated to explain laboratory airborne fungal concentrations. A total of 97 air samples were collected from 10 facilities in two institutions. Aspergilus spp., including Aspergilus niger, Aspergillius flavos and Penicillium spp. were found as predominant species. Airborne fungal concentrations ranged from not detected (ND) to 1,890 CFU/$m^3$. Airborne fungal concentrations were high in general-ventilated facilities and in laboratories where relative humidity ( > 60 %) were high ( p < 0.001). Therefore, we suggest that relative humidity should be maintained to properly reduce the concentration of fungal in university and hospital laboratories.

• 한국환경보건학회지
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• 제40권2호
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• pp.81-87
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• 2014

Objectives: We measured the concentrations of culturable airborne fungi (CAF) in enclosed environments at 16 underground subway stations of the Seoul Metro in 2013, and investigated the effect of environmental factors, including temperature, relative humidity, the number of passengers, and distance from the platform. Methods: The cultured fungi were identified by the lactophenol cotton blue (LPCB) staining method and were classified by observing the form, shape, and color of colony. A nonparametric analysis was used to determine if the differences in the concentrations of CAF were statistically significant. Results: The concentrations of CAF at the stations were the highest in station p ($367CFU/m^3$) with arange between 3 and $437CFU/m^3$. There was a significant correlation between CAF concentration and the distance from platform (r = 0.544, p < 0.01). Geotrichum spp. and Penicillium spp. were the predominant species. Conclusion: It is recommended that special attention be given during rush hour, which is in the morning (08:00-10:00) and in the early evening (18:00-19:00) to improve the indoor air quality of the subway stations.

• 한국환경보건학회지
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• 제19권1호
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• pp.30-36
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• 1993

A survey was conducted to measure concentration of airborne microbe in a hospital using RSC air sampler during October~November 1991.The result was as follows: 1) In an agar strip GK-A media for total counts of microbial particles. The highest count were 1384 CFU/m$^3$ in the main lobby, followed by 912 CFU/m$^3$, in the obstetric room, 688 CFU/m$^3$ in 1CU. By gram staining, the distribution for organisms in the air were shown 74.1% in gram possitive cocci followed by 16.8%, in gram possitive bacilli 6.7% in gram negative bacilli and 4.7% in yeast, but low organism was detected in recovery room with 194 CFU/m$^3$. 2) In agar strip S media for Staphylococci the count at the main lobby was detected in the recovery room with 92 CFU/m$^3$, Tests of coagulase were negative Staphylococci with 78%, and positive Staphylococci with 22%. The Staphylococci were highly resistance to penicillin, ampicillin and sensitive to amikacin, cefazolin, gentamycin and chloramphenicol. 3) In agar strip C media for coliform bacteria the colony counts at the main lobby was 139 CFU/m$^3$ and treatment room was 190 CFU/m$^3$, most frequently isolated microorganisms were non fermentative bacilli. 4) In agar strip HS media for yeast and molds. Most frequently colony counts 17~76 CFU/m$^3$, 0.5% lactophenol cotton blue stains were shown unidentified 77.2%, 8.1%, in Penicillium 8.1% in Aspergillus, and 3.8% in mucor.