• 한국미생물·생명공학회지
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• 제22권5호
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• pp.467-476
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• 1994

Lactobacillus casei HY 2782, prophage cured strain was characterized to be stable as much as L casei YIT 9018, parent strain. By southern hybridization, it was confirmed that the temperate phage was incorporated in chromosomal DNA of L. casei YIT 9018 as a prophage. It was also proved that the prophage was cured from chromosomal DNA of L casei HY 2782. The growth rate, lactic acid producing ability, carbohydrates fermentation, and enzymatic activity of L. casei HY 2782 were found to be similar to those of L. casei YIT 9018. When L casei HY 2782 was used as a host, the multiplicity of infection (M.O.I.) of the temperate phage for L. casei HY 2782 was 1.0~5.0. Restriction enzyme analysis of pLC90 plasmid from L. casei HY 2782 was shown that the size was an approximately 68.22 kb. The plasmid profiles, genomic DNA patterns, and cellular fatty acids composition of L. casei HY 2782 were similar to those of L casei YIT 9018. And the major fatty acids composition of these strains were C$_{14;0}$,C$_{16;1}$, C$_{16;0}$, C$_{18;1}$ and C$_{19;cyclo-}$ 10 sets of arbitrary primer in the PCR were screened to find differentiation against two strains of L. casei. Among them, b$_{5}-1/17-1 primer was produced an approximately 1.3 kb DNA band of only L casei YIT 9018. And b$_{5}-2/17-2 primer was produced an approximately 1.0 kb DNA band of only L casei HY 2782.

• 한국미생물·생명공학회지
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• 제24권1호
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• pp.37-43
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• 1996

Augmentation of antitumor activity of antitumor drugs in combination with Lactobacillus casei HY2782 (LC2782) was studied against Sarcoma-180 (S-180) and Lewis lung carcinoma (3LL). Antitumor drugs used in this study were 5-fluorouracil (5-fu) and cyclophosphamide (CP). The prolongation effect of LC2872 on the life span of mouse intraperitoneally implanted with S-180 was stronger than that of OK-432 and BCG, while the inhibitory effect of OK-432 and BCG on the growth of 3LL solid tumor was a little stronger than that of LC2782. Average survival rates of mice administrated LC2782, OK-432 and BCG were 192%, 141%, and 112%, respectively, when that of the control was 100%, Intralesional administration of 5-Fu, CP, 5-Fu+LC2782 and CP+LC2782 resulted in 93%, 69%, 99% and 73% inhibition rates against 3LL solid tumor proliferation. The combination therapy of 5-Fu or CP with LC2782 significantly prolonged the life span of S-180-inoculated ICR mice. Average survival rates of mice administrated 5-Fu and CP alone were 115% and 99%. Furthermore, survival rates of mice administrated 5-Fu and CP in combination with LC2782 were 226% and 244%, respectively.

• Asian-Australasian Journal of Animal Sciences
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• 제17권11호
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• pp.1582-1585
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• 2004

The antioxidative ability on the basis of reduced glutathione sulphydryl level, the inhibition activities of linoleic acid peroxidation of cell free extract of Lactobacillus spp. and the effects of types of media and growth phase of the cells on the cellular GSH level have been determined. Correlation between reduced glutathione sulphydryl level and antioxidative ability of Lactobacillus spp. was analyzed: Lactobacillus casei HY 2782 contained 25.15 $\mu$mole/g of GSH, the cellular GSH level of L. casei HY 2782 reached maximum after 24 h of cultivation and tended to decrease on further cultivation up to 72 h. There was a significantly higher level of cellular GSH when grown in de Man Rogosa and Sharpe (MRS) broth than in tryptone phytone yeast extract (TPY) broth or bromcresol pruple dextrose (BCP) broth (p<0.05). The antioxidant activity of cell free extract of Lactobacillus spp. have been shown to be significantly different among strains in the inhibition of linoleic acid peroxidation by thiobarbituric acid (TBA) test (p<0.01). L. casei HY 2782 and L. acidophilus ATCC 4356 revealed a high degree of antioxidative effect in linoleic acid oxidation system. Spearmans' rank correlation coefficient between inhibitory activity on linoleic acid peroxidation and cellular GSH levels of Lactobacillus spp. was 0.65, which means a significant positive correlation.

• 한국미생물·생명공학회지
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• 제22권2호
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• pp.210-216
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• 1994

Phage utilizing medium and BL agar supplemented with antibiotic Tc(tetracycline) were developed as selective media for the isolation and counting of bifidobacteria in dairy products. The former was based on the host specificity of phage. When bifidobacteria and Lactobacillus casei HY 2782 were mixed together in dairy product, L. casei HY 2782 was laysed by J1 phage which has host specificity to L. casei HY 2782 whereas bifidobacteria grew well on the selective medium added wIth J1 phage. The latter was found to inhibit the growth of S. salivarius subsp. thermophilus, L. acidophilus, and L. casei, but commercial bifidobacteria grew well in Tc-containing BL agar.

• Journal of Animal Science and Technology
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• 제48권5호
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• pp.719-726
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• 2006

본 실험은 가수분해 시킨 유청단백질과 Lb. casei HY2782의 세포 추출물을 인간 전립선 세포에처리하여 세포 내 glutathione 농도 상승효과와 산화제 tbutyl hydroperoxide (TBHP)에 의한핵산 손상 방지 효과를 알아보기 위한 실험으로 전립선 세포 RWPE1 cells와 PC3MMM2 cells에 hydrolyzed WPI(500g/m)를 48시간 동안 처리 시, 가수분해 시키지 않은 WPI 보다 각각 28.2%, 38.4%씩 GSH 농도가 증가하는 것을 유의적으로 확인할 수 있었고(p<0.05), 카제인의 경우 RWPE1 cells and PC3MMM2 cells에 처리 시 가수분해 시킨 카제인과의 유의차가 보이지 않았으며 (p<0.05), glutathione 합성저해제인 Buthionine sulfoximin (BSO) 처리 시 Glutathione 농도가 현저하게 낮아지는 것을 확인할 수가 있었다(Anderson, 1998). 전립선 세포 RWPE1 cells 와 PC3MMM2 cells에 Lb. casei HY2782 세포 추출물 처리 시 PC3MMM2 cell에서는 GSH의 농도가 유의적으로 증가하는 것을 확인 할 수 있었고(p<0.05), RWPE1 cell에서는 증가 성향을 확인 할 수가 있었다.가수분해 시킨 유청단백질과 Lb. casei cell 세포추출물 처리 시 산화제에 의한 세포의 DNA 손상 억제정도와 사멸율의 변화를 확인 한 결과, 가수분해 WPI를 처리 후 Oxidant tbutyl hydroperoxide (TBHP)(500mM)를 이용하여 PC3MMM2 세포에 산화적인 스트레스를 주었을 시, TBHP만 처리한 구에서는 62.0%의 생존율을 나타내었고, glutathione 합성저해제인 BSO를 첨가시킨 처리구에서는 33.7%의 생존율을 나타내었다. 가수분해 시킨 WPI와 카제인 처리구에서는 각각 86.7%, 63.6%의 생존율을 나타내었고, WPI와 BSO를 함께 처리한 구에서는 71.5%의 생존율을 나타내었다. 이는 BSO에 의해 GSH의 생성이 저해되어 나타난 결과로 보여지며(Anderson, 1988), 가수분해시킨 WPI 가 가수분해 시킨 카제인보다는 높은 생존율을 나타내는 것을 유의적으로 확인할 수가 있었다. (p<0.05) Lb. casei 세포 추출물처리에 의한 생존율에서는 가수분해시킨 WPI와 유사한 수준의 82.4%의 생존율을 보였다. 가수분해시킨 유청단백질과 Lb. casei cell 세포추출물에 의한 세포에서의 glutathione 농도 증가에 의해 산화제에 의한 DNA의 손상이 적었던 것으로 사료되어지고, 이로 인해 생존율이 높았던 것으로 판단된다.