• Preventive Nutrition and Food Science
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• 제21권2호
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• pp.117-123
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• 2016

Bioconversion of aglycone-formed isoflavones from glycoside-formed isoflavones by commercial lactic acid bacteria in fermented soybean paste was evaluated. Enterococcus faecium KCTC 13410 showed the most resistant capacity and Lactobacillus acidophilus KCTC 3925 had a sensitive susceptibility at a high NaCl concentration (13.2%) in fermented soybean paste. Among the 5 strains tested, Lac. acidophilus KCTC 3925 showed the highest relative ratio of aglycone-formed isoflavones to total isoflavones in fermented soybean paste. Production of exopolysaccarides (EPS) by lactic acid bacteria was compared using de Man, Rogosa, and Sharpe medium containing 1% sucrose at $37^{\circ}C$ for 48 h. Among the 5 lactic acid bacteria, Lac. acidophilus KCTC 3925 and Lactobacillus rhamnosus KCTC 3929 were investigated to produce EPS. Based on the results concerning growing susceptibility and conversion of aglycone-formed isoflavones/EPS production, it is anticipated that Lac. acidophilus KCTC 3925 may be used for preparation of Cheonggukjang, which contains relative low NaCl content.

• 한국산학기술학회논문지
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• 제15권9호
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• pp.5693-5699
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• 2014

사용 밀가루의 종류(강력분과 박력분) 차이가 사워도우(sourdough) 제조시 품질에 미치는 영향을 분석하였다. 종균으로는, Bifidobacterium longum KCTC 5734, Enterococcus faecium KCTC 13410와 Lactobacillus acidophilus KCTC 3925을 사용하였고 효모균주는 Saccharomyces cerevisiae 였다. 혼합 프로바이오틱스와 효모균에 각각 강력분 또는 박력분 밀가루를 첨가하여 $30^{\circ}C$에서 15시간 발효 후 반죽을 합하여 이를 $30^{\circ}C$에서 다시 10시간 발효하였다. 박력분을 사용한 사워도우에서는 발효전과 비교할 때 수소이온농도는 100배, 산도는 2배 증가하였다. 병행하여 생균수와 유산 생성량은 증가하였다. 결론적으로, 본 연구결과는 기존에 알려진 강력분과 더불어 박력분 또한 발효 사워도우 제조에 응용할 수 있음을 보여준다.

• Journal of Microbiology and Biotechnology
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• 제29권5호
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• pp.739-748
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• 2019

Cheonggukjang and chaga mushrooms have numerous health benefits, and have been used in alternative medicine. Therefore, a powder mixture of 98: Cheonggukjang and 2: Chaga extracts was fermented with Lactobacillus acidophilus KCTC3925 (FCC) and its anti-obesity effects in high-fat diet (HFD)-induced obese mice were determined. Five-week-old male ICR mice were fed a normal diet or HFD in the presence or absence of 3% and 5% FCC by weight (n = 10 per group). After 12 weeks, the mice were sacrificed, and the serum and tissue samples were collected for analysis. Body weight and epididymal fat pad weight were significantly lowered in the 3% and 5% FCC groups compared with those in the HFD control group (p < 0.01). FCC supplementation suppressed serum triglyceride and increased serum HDL-C levels (p < 0.01). Serum GOT, GPT, and leptin levels, hepatic COX-2 mRNA expression, and splenic COX-2 and IL-4 mRNA expression were significantly higher in the HFD groups than in the control group (p > 0.05); however, except for splenic IL-4 levels, the increases were significantly attenuated by FCC supplementation. Expression of ICAM-1, an aortic inflammatory marker, was significantly increased in the HFD group; this effect was suppressed in the 3% FCC group (p < 0.01) but not in the 5% FCC group. FCC suppressed the body weight and epididymal fat pad weight gain, as well as inflammatory responses in the liver and spleen of HFD-fed mice. Thus, FCC supplementation will be beneficial for the treatment of obesity-related effects.