• Water Engineering Research
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• v.1 no.4
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• pp.309-320
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• 2000

In this study, laboratory study of the behavior of wastewater discharged from the Boston ocean outfall was compared with the predictions of mathematical models. The dta sets cover broad ranges of discharge conditions and oceanic conditions, and are associated with a typical type of outfall discharges with multiport diffusers. The laboratory data sets were obtained in density stratified towing tanks. These data sets were used to evaluate four commonly used models: UM, UDKHDEN, RSB and CORMI$\times$2 for minimum dilution, the height to the top of the wastefield, and wastefield thickness. For minimum diluation and height to the top of the wastefield, UM and RSB predictions agree well with laboratory data. UDKHDEN overestimated the minimum dilution and height to the top of the wastefield while CORMI$\times$2 underestimated these values. All of the model predictions for the wastefield thickness were widely scattered. about the measured values. The hydraulic model study reproduced the major features observed in the laboratory. It also afforded considerable insight into the mechanics of mixing of multiport risers which could not have been obtained either from the laboratory test or the mathematical models.

• Journal of the Korea Safety Management & Science
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• v.19 no.4
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• pp.133-140
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• 2017

The Ministry of Science, ICT and Future Planning made law for Pre-Hazard Risk Analysis in December 31, 2014 to protect researchers from continuing accidents in laboratory. Conducted before an experiment, Pre-Hazard Risk Analysis finds hazards of the experiment and rules to manage the hazards.So the Pre-Hazard Risk Analysis can support laboratory safety system by prevent accidents in laboratory. Pre-Hazards Risk Analysis is newly created system so that executors need Guidelines to perform this analysis properly. This study is to develop guide tool for Pre-Hazard Risk Analysis by analyzing other risk assessment systems; PSM, Off-site Consequence Assessment, laboratory safety system. Also, this study suggested how to establish database for Pre-Hazard Risk Assessment by analyse KRAS.

• The Plant Pathology Journal
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• v.35 no.1
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• pp.11-18
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• 2019

Gaeumannomyces graminis var. tritici is a soil borne pathogenic fungus associated with wheat roots. The accurate quantification of gene expression during the process of infection might be helpful to understand the pathogenic molecular mechanism. However, this method requires suitable reference genes for transcript normalization. In this study, nine candidate reference genes were chosen, and the specificity of the primers were investigated by melting curves of PCR products. The expression stability of these nine candidates was determined with three programs-geNorm, Norm Finder, and Best Keeper. $TUB{\beta}$ was identified as the most stable reference gene. Furthermore, the exopolygalacturonase gene (ExoPG) was selected to verify the reliability of $TUB{\beta}$ expression. The expression profile of ExoPG assessed using $TUB{\beta}$ agreed with the results of digital gene expression analysis by RNA-Seq. This study is the first systematic exploration of the optimal reference genes in the infection process of Gaeumannomyces graminis var. tritici.

• BMB Reports
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• v.40 no.2
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• pp.172-179
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• 2007

PCR-mediated recombination describes the process of in vitro chimera formation from related template sequences present in a single PCR amplification. The high levels of genetic redundancy in eukaryotic genomes should make recombination artifacts occur readily. However, few evolutionary biologists adequately consider this phenomenon when studying gene lineages. The cytosolic glyceraldehyde-3-phosphate dehydrogenase gene (GapC), which encodes a NADP-dependent nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase in the cytosol, is a classical lowcopy nuclear gene marker and is commonly used in molecular evolutionary studies. Here, we report on the occurrence of PCR-mediated recombination in the GapC gene family of Hibiscus tiliaceus. The study suggests that recombinant areas appear to be correlated with DNA template secondary structures. Our observations highlight that recombination artifacts should be considered when studying specific and allelic phylogenies. The authors suggest that nested PCR be used to suppress PCRmediated recombination.

• Laser Solutions
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• v.16 no.1
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• pp.5-9
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• 2013

We have successfully formed filament inside of a transparent soda-lime glass using a Ti:sapphire based femtosecond laser. To make filament form, keeping the laser intensity higher than critical intensity is essential. Also each of the machining parameters plays an important role for the formation of filament. In this paper, we study what parameter can possibly influence for formation of filament, and we introduce an application using filamentation by femtosecond laser for transparent material.

• Journal of Power Electronics
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• v.17 no.1
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• pp.172-180
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• 2017

The repetitive control (RC) strategy is widely used in AC power systems because of its high performance in tracking period signal and suppressing steady-state error. However, the dynamic response of RC is determined by the fundamental period delay $T_0$ existing in the internal model. In the current study, a ($nk{\pm}i$)-order harmonic RC structure is proposed to improve dynamic performance. The proposed structure has less data memory and can improve the tracking speed by n/2 times. $T_0$ proves the effectiveness of the ($nk{\pm}i$)-order RC strategy. The simulation and experiments of ($6k{\pm}1$)-order and ($4k{\pm}1$)-order RC strategy used in the voltage source inverter is conducted in this study to control the harmonic current source, which shows the validity and advantages of the proposed structure.

• Microbiology and Biotechnology Letters
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• v.47 no.3
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• pp.423-433
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• 2019

Midgut microbiota is known to play a fundamental role in the biology and physiology of the agricultural pest, Spodoptera litura. This study reports the difference in the larval midgut microbiota of field-caught and laboratory-reared populations of S. litura by performing 16S rDNA amplicon pyrosequencing. Field populations for the study were collected from castor crops, whereas laboratory-reared larvae were fed on a regular chickpea based diet. In total, 23 bacterial phylotypes were observed from both laboratory-reared and field-caught caterpillars. Fisher's exact test with Storey's FDR multiple test correction demonstrated that bacterial genus, Clostridium was significantly abundant (p < 0.05) in field-caught larvae of S. litura as compared to that in the laboratory-reared larvae. Similarly, bacterial genera, such as Bradyrhizobium, Burkholderia, and Fibrisoma were identified (p < 0.05) predominantly in the laboratory-reared population. The Bray-Curtis dissimilarity matrix depicted a value of 0.986, which exhibited the maximum deviation between the midgut microbiota of the laboratory-reared and field-caught populations. No significant yeast diversity was seen in the laboratory-reared caterpillars. However, two yeast strains, namely Candida rugosa and Cyberlindnera fabianii were identified by PCR amplification and molecular cloning of the internal transcribed space region in the field-caught caterpillars. These results emphasize the differential colonization of gut residents based on environmental factors and diet.

• Journal of Korean Elementary Science Education
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• v.38 no.2
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• pp.203-215
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• 2019

The purpose of this study is to clarify the roles of laboratory work in school. Twenty-one literatures were identified about roles of laboratory work in school by applying criteria such as published in CI level journals or used as a reference over 100 times during 1960-2017. Twenty-one literatures were reviewed according to periods such as 1960-1989, 1990-2003, and 2004-2017, and identified the roles of laboratory work in school that commonly presented in more than two literature. Seven roles of laboratory work in school identified were as follows (a) learning scientific knowledge, (b) enhancing attitude toward science, (c) learning scientific inquiry methods, (d) acquiring skills to use specific laboratory instruments, (e) enhancing scientific attitude, (f) understanding the nature of science(NOS), and (g) providing opportunity to experience natural or scientific phenomena. The results of this study can be used to provide school teachers and students a clear meaning of the roles of laboratory work in school.

• Journal of Veterinary Science
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• v.25 no.1
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• pp.15.1-15.15
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• 2024

Background: The anti-programmed death 1 (PD-1) antibody has led to durable clinical responses in a wide variety of human tumors. We have previously developed the caninized anti-canine PD-1 antibody (ca-4F12-E6) and evaluated its therapeutic properties in dogs with advance-staged oral malignant melanoma (OMM), however, their therapeutic effects on other types of canine tumors remain unclear. Objective: The present clinical study was carried out to evaluate the safety profile and clinical efficacy of ca-4F12-E6 in dogs with advanced solid tumors except for OMM. Methods: Thirty-eight dogs with non-OMM solid tumors were enrolled prospectively and treated with ca-4F12-E6 at 3 mg/kg every 2 weeks of each 10-week treatment cycle. Adverse events (AEs) and treatment efficacy were graded based on the criteria established by the Veterinary Cooperative Oncology Group. Results: One dog was withdrawn, and thirty-seven dogs were evaluated for the safety and efficacy of ca-4F12-E6. Treatment-related AEs of any grade occurred in 13 out of 37 cases (35.1%). Two dogs with sterile nodular panniculitis and one with myasthenia gravis and hypothyroidism were suspected of immune-related AEs. In 30 out of 37 dogs that had target tumor lesions, the overall response and clinical benefit rates were 6.9% and 27.6%, respectively. The median progression-free survival and overall survival time were 70 days and 215 days, respectively. Conclusions: The present study demonstrated that ca-4F12-E6 was well-tolerated in non-OMM dogs, with a small number of cases showing objective responses. This provides evidence supporting large-scale clinical trials of anti-PD-1 antibody therapy in dogs.

• Proceedings of the Korean Nuclear Society Conference
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• 2004.10a
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• pp.79-80
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• 2004