• Title/Summary/Keyword: Laboratory set-up

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Genetic Identification of the North Pacific Chum Salmon (Oncorhynchus keta) Stocks (유전적 형질에 의한 북태평양 연어 (Oncorhynchus keta)의 계군 구분)

  • JUNG Woongsic;LEE Youn-Ho;KIM Suam;JIN Deuk-Hee;SEONG Ki Baek
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.36 no.6
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    • pp.578-585
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    • 2003
  • The chum salmon (Oncorhynchus keta) is an anadromous fish distributed all around the North Pacific. Artificial production and release of the juveniles are being made by Korea, Japan, Russia, Canada and the United States. It is important to set up some criteria identifying each stock in order to clarify each nation's right of harvest for the chum salmon resource. As an attempt to build such criteria, we analyzed sequences of a microsatellite DNA Ogo5 and the COIII-ND3-ND4L region of the mitochondrial DNA from chum salmons of Korea, Japan, and the United States. Ogo5 has 4 different alleles: allele A, B-1, B-2, and B-3. Allele B-3 is found only in 3 individuals out of 12 Korea salmons. The Japan salmons have the other 3 alleles and the America salmons have only 2 allots, A and B-1. Heterozygosity index (Ho/He) distinguishes the Korea (1.61) and Japan salmons (1.63) from the America ones (1.09). Seventeen different haplotypes are found in the COIII-ND3-ND4L region from 60 individuals,20 from each stock. The gene genealogy of the haplotypes revealed by TCS program shows that the Korea and Japan salmons are genetically closely linked, but that they are clearly distinguished from the America ones. Ten and eleven individuals of the Korea and Japan salmons have an identical haplotype. Nine individuals of the Korea salmons $(45\%),$ however, are separable from the Japan salmons by their own specific nucleotides. This result presents usefulness of the COIII-ND3-ND4L region as a genetic marker for identification of the chum salmon stocks.

A Study on Simulation of Cavity and Relaxation Zone Using Laboratory Model Test and Discrete Element Method (실내모형실험과 개별요소법을 이용한 지반 공동 및 이완영역 모사에 관한 연구)

  • Kim, Joo-Bong;You, Seung-Kyong;Han, Jung-Geun;Hong, Gi-Gwon;Park, Jong-Beom
    • Journal of the Korean Geosynthetics Society
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    • v.16 no.2
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    • pp.11-21
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    • 2017
  • Ground subsidence mainly occurs due to the soil wash-away caused by cracked sewer pipes. It is necessary to understand the behavior surrounding soils with the formation of cavity and relaxation zone to set up counterplan. In this paper, a series of laboratory model tests and numerical analyses (Discrete Element Method) were performed to investigate the ground subsidence mechanism due to sewer pipe damage. For model tests, aluminum rod and trap door were used to simulate the behavior of model ground. Test results were compared with the numerical analyses conducted under the same boundary conditions with model tests. From this study, it was investigated the shape and size of cavity and relaxation zone due to the soil wash-away and a void ratio distribution of surrounding soils with relaxation properties.

Alternative and Rapid Detection Methods for Wastewater Surveillance of SARS-CoV-2 (SARS-CoV-2의 하수조사를 위한 대체 및 신속 검출 방법)

  • Jesmin Akter;Bokjin Lee;Jai-Yeop Lee;Chang Hyuk Ahn;Nishimura Fumitake;ILHO KIM
    • Journal of Korean Society on Water Environment
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    • v.40 no.1
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    • pp.19-35
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    • 2024
  • The global pandemic, coronavirus disease caused by Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has led to the implementation of wastewater surveillance as a means to monitor the spread of SARS-CoV-2 prevalence in the community. The challenging aspect of establishing wastewater surveillance requires a well-equipped laboratory for wastewater sample analysis. According to previous studies, RT-PCR-based molecular tests are the most widely used and popular detection method worldwide. However, this approach for the detection or quantification of SARS-CoV-2 from wastewater demands a specialized laboratory, skilled personnel, expensive instruments, and a workflow that typically takes 6 to 8 hours to provide results for a few samples. Rapid and reliable alternative detection methods are needed to enable less-well-qualified practitioners to set up and provide sensitive detection of SARS-CoV-2 within wastewater at regional laboratories. In some cases, the structural and molecular characteristics of SARS-CoV-2 are unknown, and various strategies for the correct diagnosis of COVID-19 have been proposed by research laboratories. The ongoing research and development of alternative and rapid technologies, namely RT-LAMP, ELISA, Biosensors, and GeneXpert, offer a wide range of potential options not only for SARS-CoV-2 detection but also for other viruses. This study aims to discuss the effective regional rapid detection and quantification methods in community wastewater.

Dynamic Service Composition and Development Using Heterogeneous IoT Systems

  • Ryu, Minwoo;Yun, Jaeseok
    • Journal of the Korea Society of Computer and Information
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    • v.22 no.9
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    • pp.91-97
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    • 2017
  • IoT (Internet of Things) systems are based on heterogeneous hardware systems of different types of devices interconnected each other, ranging from miniaturized and low-power wireless sensor node to cloud servers. These IoT systems composed of heterogeneous hardware utilize data sets collected from a particular set of sensors or control designated actuators when needed using open APIs created through abstraction of devices' resources associated to service applications. However, previously existing IoT services have been usually developed based on vertical platforms, whose sharing and exchange of data is limited within each industry domain, for example, healthcare. Such problem is called 'data silo', and considered one of crucial issues to be solved for the success of establishing IoT ecosystems. Also, IoT services may need to dynamically organize their services according to the change of status of connected devices due to their mobility and dynamic network connectivity. We propose a way of dynamically composing IoT services under the concept of WoT (Web of Things) where heterogeneous devices across different industries are fully integrated into the Web. Our approach allows developers to create IoT services or mash them up in an efficient way using Web objects registered into multiple standardized horizontal IoT platforms where their resources are discoverable and accessible. A Web-based service composition tool is developed to evaluate the practical feasibility of our approach under real-world service development.

Full-scale experimental verification on the vibration control of stay cable using optimally tuned MR damper

  • Huang, Hongwei;Liu, Jiangyun;Sun, Limin
    • Smart Structures and Systems
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    • v.16 no.6
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    • pp.1003-1021
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    • 2015
  • MR dampers have been proposed for the control of cable vibration of cable-stayed bridge in recent years due to their high performance and low energy consumption. However, the highly nonlinear feature of MR dampers makes them difficult to be designed with efficient semi-active control algorithms. Simulation study has previously been carried out on the cable-MR damper system using a semi-active control algorithm derived based on the universal design curve of dampers and a bilinear mechanical model of the MR damper. This paper aims to verify the effectiveness of the MR damper for mitigating cable vibration through a full-scale experimental test, using the same semi-active control strategy as in the simulation study. A long stay cable fabricated for a real bridge was set-up with the MR damper installed. The cable was excited under both free and forced vibrations. Different test scenarios were considered where the MR damper was tuned as passive damper with minimum or maximum input current, or the input current of the damper was changed according to the proposed semi-active control algorithm. The effectiveness of the MR damper for controlling the cable vibration was assessed through computing the damping ratio of the cable for free vibration and the root mean square value of acceleration of the cable for forced vibration.

Estimation of Optimal Weight in Tidal Modeling with the Adjoint Method (조석 모델링에서 adjoint 방법 적용시 적정 가중치 산정)

  • Lee, Jae-Hak;Park, Kyeong;Song, Yong-Sik
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.5 no.3
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    • pp.177-185
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    • 2000
  • The adjoint method is a method of data assimilation to improve the model results by seeking for model parameters that minimize the cost function and satisfy the governing equations of a model simultaneously. An adjoint package was set up for the two-dimensional linear tidal model and was applied to an idealized domain for an optimal estimation of the open boundary conditions. The assimilating data were selected from the results of forward modeling. Attention is paid on the response of the adjoint package to weighting parameters, the importance of initial estimates of model parameters and the applicability of the adjoint package to the case with varying depth. A procedure to determine optimal weight is presented based on the relationships between weights and other factors.

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An Experimental Study on the Variation of Pore Water Pressures in the Seabed Subjected to Waves (파랑하중에 의한 해저지반의 공극수압 변화에 대한 연구)

  • 장병욱;강준영
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.38 no.5
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    • pp.85-94
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    • 1996
  • For the geotechnical analysis in the construction and Deign of the coastal structures, one of the most important factors is the existence of waves. The dynamic behavior and deformation of the seabed subjected to wave load must be considered. It is expected that the soil behavior in the seabed subjected to cyclic wave load is much different from that on the ground subjected to dynamic forces such as earthquake. The purposes of this study are as follows ; Firstly, to provide a testing method to generate wave loads in the laboratory and measuring oscillatory pore water pressures in the unsaturated marine silty sand specimen, Secondly, to analyze the mechanism of wave induced pore water pressures and liquefaction potentials under the conditions in the testing. It is shown that the test set-up manufactured especially for the test is good to generate oscillatory wave pressures to the specimen with sine wave type. From the results of this study, it is understood that the pore water pressure due to induced waves is not accumulated as the wave number increases but is periodically varied with wave passage on still water surface. The magnitude of pore water pressures measured tends to be diminished radically with a certain time lag under the action of both high and low waves as depth increases.

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A Study on the Measurement of Small Partial Discharges in a Low-voltage Induction Motor (저압 유도전동기에서 미소 부분방전 측정에 관한 연구)

  • Kil, Gyung-Suk;Song, Jae-Yong;Seo, Hwang-Dong;Hwang, Don-Ha;Kang, Dong-Sik;Kim, Yong-Joo
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.18 no.10
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    • pp.960-964
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    • 2005
  • In this paper, we dealt with a partial discharge(PD) measurement method that has been accepted as an effective and non-destructive technique to estimate insulation performance of low-voltage electric and electronic devices. The PD measurement system Is composed of a coupling network, a low noise amplifier, and associated electronics. h shielded box Is adopted to make a better measurement environment against around noise. A low cut-off frequency of the coupling network estimated by a sinusoidal wave input was 125 kHz (-3 dB). Calibration tests on laboratory set-up have shown that the PD measurement system has a stable sensitivity of 10 mV/pC. In an application test oil a low-voltage three phase Induction motor (5 HP), we could detect 0.53 pC level of partial discharge at the applied voltage of AC 650 $V_{peak}$.

Three-phase current type PWM converter using resonant DC Link snubber (공진 DC 링크 스너버를 이용한 3상 전류형 PWM 컨버터)

  • Suh, Ki-Youn;Lee, Hyun-Woo;Lee, Soo-Heun;Mun, Sang-Pil;Kim, Young-Mun
    • Proceedings of the KIEE Conference
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    • 2001.07b
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    • pp.1015-1019
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    • 2001
  • This paper presents a novel three-phase current-fed Pulse Width Modulation converter with switched capacitor type resonant DC link commutation circuit operating PWM pattern strategy under a design consideration of low-pass filter, which can operate on the basis of the principle of zero current soft switching commutation. In the first place, the steady state operating principle of this converter with a new resonant DC link snubber circuit is described in connection with the equivalent operation circuit, together with the practical design procedure of the switched-capacitor type resonant DC link circuit is discussed from a theoretical viewpoint on the basis of a design example for high-power applications. The actively delayed time correction method to compensate distorted currents due to a relatively long resonant commutation time is newly implemented in the open loop control scheme so as to acquire the new optimum PWM pattern. Finally, the experiment of set-up in laboratory system of this converter is concretely demonstrated herein to confirm a zero current soft-switching commutation of this converter. The comparative evaluations between current-fed hard switching PWM and soft-switching PWM converters are carried out from a viewpoint of their PWM converter characteristics.

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Investigation and Repair Methods for the Excavated Mountain Area damaged by Rainfall : At the Sunghak Campus, Dong-A University (호우로 인한 절개산지의 피해 조사 및 대책공법 -동아대 승학 캠퍼스를 중심으로-)

  • 정성교;김종대
    • Geotechnical Engineering
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    • v.7 no.2
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    • pp.27-40
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    • 1991
  • The Sunghak Campus, Dong-A University, which located at the excavated area of mountain, has been constructed year by year for about 10 years since 1978. The buildings, steep slopes, road and so on in the Campus area were damaged by heavy rainfall more than 200mm 1 day on August, 1989. The methods used for the investigation and repair methods of the damage are the preliminary investigation, the present condition survey, 50 by 50m grid survey, compasstraverse for locating outcrops, geopysical exploration, geologic survey, boring and laboratory soil testing. Based on the results of investigations, the causes of the failures have been evaluated, and the repair methods have been set up as horizontal drains, removal of the dangerous rock, retaining wall with subsurface drains, the elimination of colluvium and slope stabilization by use of precast concrete grids and so on.

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