• Asian Pacific Journal of Cancer Prevention
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• 제14권10호
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• pp.6069-6075
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• 2013

Background: At present, the diagnosis of colorectal cancer (CRC) requires a colorectal biopsy which is an invasive procedure. We undertook this pilot study to develop an alternative method and potential new biomarkers for diagnosis, and validated a set of well-integrated tools called ClinProt to investigate the serum peptidome in CRC patients. Methods: Fasting blood samples from 67 patients diagnosed with CRC by histological diagnosis, 55 patients diagnosed with colorectal adenoma by biopsy, and 65 healthy volunteers were collected. Division was into a model construction group and an external validation group randomly. The present work focused on serum proteomic analysis of model construction group by ClinProt Kit combined with mass spectrometry. This approach allowed construction of a peptide pattern able to differentiate the studied populations. An external validation group was used to verify the diagnostic capability of the peptidome pattern blindly. An immunoassay method was used to determine serum CEA of CRC and controls. Results: The results showed 59 differential peptide peaks in CRC, colorectal adenoma and health volunteers. A genetic algorithm was used to set up the classification models. Four of the identified peaks at m/z 797, 810, 4078 and 5343 were used to construct peptidome patterns, achieving an accuracy of 100% (> CEA, P<0.05). Furthermore, the peptidome patterns could differentiate the validation group with high accuracy close to 100%. Conclusions: Our results showed that proteomic analysis of serum with MALDI-TOF MS is a fast and reproducible approach, which may provide a novel approach to screening for CRC.

• 한국축산시설환경학회지
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• 제19권1호
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• pp.47-54
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• 2013

Two types of reactors were set to investigate the change of characteristics of pig slurry by aeration during fertilization period. One system was equipped with air diffuser to supply oxygen to pig slurry for liquid fertilization, but there was no air diffuser in the other system. Air supply to the experimental systems was regulated by air flow meter. The reactors were set up in the laboratory to protect the pig slurry from external condition such as temperature and humidity changes. Maintaining optimal pH range in the experimental reactors is an important factor for liquid fertilization of pig slurry. In this study, pH ranges of aerobic reactor and anoxic reactor was 7.04~7.19 and 7.34~7.81, respectively. The temperature of aerobic reactors was $2{\sim}3^{\circ}C$ higher then indoor temperature. The amount of sludge accumulated at the bottom layer of non-aerated reactors was 4~5 times more than that of aerated reactors.

• 한국도로학회논문집
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• 제10권2호
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• pp.15-26
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• 2008

최근 들어 철근 부식, 콘크리트 포장표면의 박리파손, 환경 피해 등 염화물계 제설제의 사용에 따른 각종 피해가 보고되고 있으며, 이를 위해 대체 제설제의 개발을 위한 연구가 활발히 진행되고 있다. 하지만 개발된 제설제의 성능평가에 대한 연구는 대부분 실내실험을 위주로 한 기초적이고 제한적인 성능평가에 의존하고 있는 선정이다. 특히 제설제의 융빙성능은 제설제가 갖추어야 할 기본적인 성능으로, 합리적이고 체계적인 평가방법을 통한 융빙성능의 평가가 필수적이다. 본 연구에서는 기존 연구를 바탕으로 보다 구체적이고 현실적인 융빙성능 평가방법을 수립하였으며 그 절차에 따라 본 연구진에 의해 개발된 저염화물계 친환경 제설제인 EFD(Eco Friendly Deicer)의 융빙성능을 평가 및 검증하였다. 실내실험 결과, EFD는 국내 고속도로에서 사용 중인 습염식 제설제와 대등한 수준의 초기 융빙성능을 나타냈으며 2회의 현장살포 실험을 통해 EFD의 융빙성능을 검토한 결과, 전체적인 융빙성능이 습염식 제설제의 융빙 성능에 비해 큰 차이가 없음을 확인하였다.

• 한국동물학회지
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• 제7권2호
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• pp.41-47
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• 1964

D.auraria is a species belonging to the D.melanogaster group, and this species was divided into 3 races(A, B and C race ) by morphoogical difference of the genitals . Korean populations of D.auraria A race are polymorphic with respect to the pigmentation of the abdominal tergites. The female shows two forms of color pattern, dark and light, on the sixth to the ninth tergites, and the male has no distinction shown by the female. Crossing experiments in the laboratory have shown that the difference between these color forms is due to a single pair of allelic genes located on an autosome. In natural populations, the light forms of females are always commoner than the dark one. The number of the light form increases relatively in southern localities and decreases relatively in northern localities. Furthermore, the number of the light form increases relatively in summer and decreases relatively in fall and spring. By genetic analyses of these wild flies, the relation, d/d>d/D>D/D has been disclosed as regards the relative frequencies of the genotypes in natural populations. Three experimental populations have been set up in the laboratory. Homozygous dark forms and homozygous light forms were mixed together and cultured in population cage at the temperature of 25 $^{\circ}C$. Approximately one year later, the frequencies of the light and the dark forms reached an equilibrium , the light one being usually more frequent than the dark one. This indicates that the heterozygous dark form possesses the highest adaptive value, the homozygous light being intermediate and the homozygous dark lowest. In number of adults hached, the homozygous light form was superior to the heterozygous dark form and the homozygous dark form, but the differences are scarcely significant.

• 한국수산과학회지
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• 제38권4호
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• pp.265-275
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• 2005

A prerequisite for deriving the abundance estimates from acoustic surveys for commercially important fish species is the identification of target strength measurements for selected fish species. In relation to these needs, the goal of this study was to construct a data bank for converting the acoustic measurements of target strength to biological estimates of fish length and to simultaneously obtain the target strength-fish length relationship. Laboratory measurements of target strength on 15 commercially important fish species were carried out at five frequencies of 50, 70, 75, 120 and 200 kHz by single and split beam methods under the controlled conditions of the fresh and the sea water tanks with the 389 samples of dead and live fishes. The target strength pattern on individual fish of each species was measured as a function of tilt angle, ranging from $-45^{\circ}$ (head down aspect) to $+45^{\circ}$ (head up aspect) in $0.2^{\circ}$ intervals, and the averaged target strength was estimated by assuming the tilt angle distribution as N $(-5.0^{\circ},\;15.0^{\circ})$. The TS to fish length relationship for each species was independently derived by a least-squares fitting procedure. Also, a linear regression analysis for all species was performed to reduce the data to a set of empirical equations showing the variation of target strength to a fish length, wavelength and fish species. For four of the frequencies (50, 75, 120 and 200 kHz), an empirical model for fish target strength (TS, dB) averaged over the dorsal sapect of 602 fishes of 10 species and which spans the fish length (L, m) to wavelength (\Lambda,\;m)$ ratio between 5 and 73 was derived: $TS=19.44\;Log(L)+0.56\;Log(\Lambda)-30.9,\;(r^2=0.53)$.

• Journal of the Optical Society of Korea
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• 제20권1호
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• pp.78-87
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• 2016

Red blood cells (RBCs) are customarily adhered to a bio-functionalised substrate to make them stationary in interferometric phase-imaging modalities. This can make them susceptible to receive alterations in innate morphology due to their own weight. Optical tweezers (OTs) often driven by Gaussian profile of a laser beam is an alternative modality to overcome contact-induced perturbation but at the same time a steeply focused laser beam might cause photo-damage. In order to address both the photo-damage and substrate adherence induced perturbations, we were motivated to stabilize the RBC in OTs by utilizing a laser beam of ‘arbitrary intensity profile’ generated by a source having cavity imperfections per se. Thus the immobilized RBC was investigated for phase-imaging with sinusoidal interferograms generated by a compact and robust Michelson interferometer which was designed from a cubic beam splitter having one surface coated with reflective material and another adjacent coplanar surface aligned against a mirror. Reflected interferograms from bilayers membrane of a trapped RBC were recorded and analyzed. Our phase-imaging set-up is limited to work in reflection configuration only because of the availability of an upright microscope. Due to RBC’s membrane being poorly reflective for visible wavelengths, quantitative information in the signal is weak and therefore, the quality of experimental results is limited in comparison to results obtained in transmission mode by various holographic techniques reported elsewhere.

• 한국조경학회지
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• 제31권4호
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• pp.74-81
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• 2003

The need for insect and mite resistant turfgrass cultivars arose because of problems associated with pesticide use. Representative cultivars and genotypes of 18 warm-season turfgrass [Zoysia japonica Steud., Z. japonica${\times}$Z. metrella hybrids, Z. japonica${\times}$Z. tenuifotia hybrids, Z. matrella (L.) Merr., Cynodon dactylon (L.) Pers., C. dactylon${\times}$C. transvallensis hybrids, Paspalum notatum Flugge., P. vaginatum Swartz., Stenotaphrum secundatum (Walt.) Kuntze, Eremochloa ophiuroides (Munro.) and Buchloe dactyloides (Nutt.) Engelm.] and 20 cool-season turfgrasses [Poa pratensis L., Festuca arundinacea Schreb., F. rubra L., F. rubra var. commutata Gaud., F. ovina var. duriuscula L. Koch. Agrostis tenuis Sibth., A. palustris Huds., and Latium perenne L.] were evaluated for host resistance to feeding by the Spodoptera depravata (Butler) in the laboratory. Two experiments were set up in the laboratory using 8.5cm diameter${\times}$4.0cm deep plastic petri dishes as larvae feeding chambers. In experiment 1, one neonate larvae were place on the grass in each dish and the dishes were arranged with 5 replicates each within an environmental chamber maintained at $25^{\circ}C$ and 15h light: 9h dark Larval survival and larval weights at 7d and 14d, pupal weights, and days to pupation were compared among turfgrasses. In Experiment 2, 4cm sections of all grasses were oriented equidistant from each other in a pattern resembling the spokes of a wheel. Five one neonate larvae were introduced to the center of each dish. Dishes were immediately placed in an environmental chamber held at $25^{\circ}C$, 15h light: 9h dark Larvae were allowed to feed for 24h. Damage was rated from 0(no damage) to 9(completely consumed) were made for eachturfgrass. Resistance as antibiosis (high mortality, slowed growth, and least preference) was identified in Z. japonica${\times}$Z. tenuifolia hybirids ‘Emerald’, Z. japonica${\times}$Z. metrella hybirds ‘Miyako’ and Eremochloa ophiuroides (Munro.). Cool-season turfgrasses tested were susceptible to feeding by Spodoptera depravata (Butler).

• 한국펄프종이공학회:학술대회논문집
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• 한국펄프종이공학회 2006년도 PAN PACIFIC CONFERENCE vol.2
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• pp.215-225
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• 2006

Today's requirements for print-press runnability and print quality demand an optimised absorption and adhesion of printing ink on the paper surface. Modern coating concepts for high glossing offset grades use ultra fine pigments, whereas binder level has continuously been decreased to a minimum in recent years to achieve the highest possible sheet gloss development and for economical reasons. Both the ultra fine pigments and the reduced binder levels lead in many cases to a faster ink setting rate. On the other hand, matt paper grades use relatively coarse pigments leading to a slow ink setting compared to the high glossing papers. Both too fast and too slow ink setting properties implicate drawbacks in print quality and print press runnability. The mechanisms behind the interactions between ink and coating have been presented in many previous publications. The purpose of this study was to determine and quantify how the ink setting rate is influenced by pigment system (GCC and GCC/clay blends), latex level and latex properties in the topcoat of double coated sheet fed offset paper. The roles of binder level and type in the precoat were also assessed. The effect of calendering (temperature and pressure) was studied with one formulation. The resulting ink setting characteristics were tested using three different laboratory testing instruments. The correlation amongst the different laboratory testing methods is discussed. The results show that by varying the latex properties, the pigment system and/or latex addition level, the ink tack development of ink applied to a topcoat pigment system can be significantly influenced. It can be slowed down as often desired with ultra fine pigments or speeded up in the case of coarse pigments. There was no visible effect on the ink setting rate by using different binder systems in the precoat..

• 수산해양기술연구
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• 제41권4호
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• pp.296-305
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• 2005

Acoustic target strength (TS) of 12 commercially important fish species caught in the Korean waters had been investigated and their results were presented. Laboratory measurements of target strength on 12 dominant fish species were carried out at a frequencies of 75 kHz by single beam method under the controlled condition of the water tank with the 241 samples of dead and live fishes. The target strength pattern on individual fish of each species was measured as a function of tilt angle, ranging from $-45^{\circ}$ (head down aspect) to $45^{\circ}$ (head up aspect) in $0.2^{\circ}$ intervals, and the averaged target strength was estimated by assuming the tilt angle distribution as N ($-5.0^{\circ}$, $^15.0{\circ}$). The 75 to fish length relationship for each species was independently derived by a least - squares fitting procedure. Also, a linear regression analysis for all species was performed to reduce the data to a set of empirical equations showing the variation of target strength to fish length and fish species. An empirical model for fish target strength(TS, dB) averaged over the dorsal aspect of 158 fishes of 7 species and which spans the fish length(L, m) to wavelength(${\lambda}$, m) ratio between 6.2 and 21.3 was derived: TS: 27.03 Log(L)-7.7Log(${\kanbda}$)-17.21, ($r^2$=0.59).

• 한국수산과학회지
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• 제36권6호
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• pp.578-585
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• 2003

The chum salmon (Oncorhynchus keta) is an anadromous fish distributed all around the North Pacific. Artificial production and release of the juveniles are being made by Korea, Japan, Russia, Canada and the United States. It is important to set up some criteria identifying each stock in order to clarify each nation's right of harvest for the chum salmon resource. As an attempt to build such criteria, we analyzed sequences of a microsatellite DNA Ogo5 and the COIII-ND3-ND4L region of the mitochondrial DNA from chum salmons of Korea, Japan, and the United States. Ogo5 has 4 different alleles: allele A, B-1, B-2, and B-3. Allele B-3 is found only in 3 individuals out of 12 Korea salmons. The Japan salmons have the other 3 alleles and the America salmons have only 2 allots, A and B-1. Heterozygosity index (Ho/He) distinguishes the Korea (1.61) and Japan salmons (1.63) from the America ones (1.09). Seventeen different haplotypes are found in the COIII-ND3-ND4L region from 60 individuals,20 from each stock. The gene genealogy of the haplotypes revealed by TCS program shows that the Korea and Japan salmons are genetically closely linked, but that they are clearly distinguished from the America ones. Ten and eleven individuals of the Korea and Japan salmons have an identical haplotype. Nine individuals of the Korea salmons $(45\%),$ however, are separable from the Japan salmons by their own specific nucleotides. This result presents usefulness of the COIII-ND3-ND4L region as a genetic marker for identification of the chum salmon stocks.