• Journal of Microbiology and Biotechnology
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• 제20권1호
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• pp.21-29
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• 2010

The phenolic compounds are a major contaminant class often found in industrial wastewaters and the biological treatment is an alternative tool commonly employed for their removal. In this sense, monitoring microbial community dynamics is crucial for a successful wastewater treatment. This work aimed to monitor the structure and activity of the bacterial community during the operation of a laboratory-scale continuous submerged membrane bioreactor (SMBR), using PCR and RT-PCR followed by denaturing gradient gel electrophoresis (DGGE) and 16S rRNA libraries. Multivariate analyses carried out using DGGE profiles showed significant changes in the total and metabolically active dominant community members during the 4-week treatment period, explained mainly by phenol and ammonium input. Gene libraries were assembled using 16S rDNA and 16S rRNA PCR products from the fourth week of treatment. Sequencing and phylogenetic analyses of clones from the 16S rDNA library revealed a high diversity of taxa for the total bacterial community, with predominance of Thauera genus (ca. 50%). On the other hand, a lower diversity was found for metabolically active bacteria, which were mostly represented by members of Betaproteobacteria (Thauera and Comamonas), suggesting that these groups have a relevant role in the phenol degradation during the final phase of the SMBR operation.

• 한국수산과학회지
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• 제9권1호
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• pp.35-42
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• 1976

1974년부터 1975년 말까지 부안만 대합에 기생하는 흡충류 유충의 발육, 기생율, 조직변화 및 사육실험을 통하여 대합의 폐사원인을 밝혔다. 1. 대합의 생식소, 중장선, 새엽에 기생하는 유모 cercaria인 Cercaria pectinata Huet와 그 sporocyst를 검출하였다. 2. 기생충의 수와 량이 급히 증가하므로 흡충류에 기생된 대합은 기능장해를 받아 폐사된다. 3. 부안산 대합 2,639개체에 대한 대합에 기생되는 흡충의 평균 기생율은 $13.2\%$였는데, 월별로 보면 7월이 가장 높았으며$(29.6\%)$, 3월이 가장 낮았다$(2.0\%)$. 한편 각장에 관계없이 기생율은 거의 같았다. 4. 흡충류에 기생된 대합의 생식소는 거세되고, 유충의 증식으로 중장선은 압박을 받으며 새판내에 기생되므로 혈액순환장해를 일으킨다. 5. 수조 내에서 사육한 기생패는 35일만에 전부 폐사했다.

• Nuclear Engineering and Technology
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• 제38권3호
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• pp.239-250
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• 2006

Computational anthropomorphic phantoms are computer models of human anatomy used in the calculation of radiation dose distribution in the human body upon exposure to a radiation source. Depending on the manner to represent human anatomy, they are categorized into two classes: stylized and tomographic phantoms. Stylized phantoms, which have mainly been developed at the Oak Ridge National Laboratory (ORNL), describe human anatomy by using simple mathematical equations of analytical geometry. Several improved stylized phantoms such as male and female adults, pediatric series, and enhanced organ models have been developed following the first hermaphrodite adult stylized phantom, Medical Internal Radiation Dose (MIRD)-5 phantom. Although stylized phantoms have significantly contributed to dosimetry calculation, they provide only approximations of the true anatomical features of the human body and the resulting organ dose distribution. An alternative class of computational phantom, the tomographic phantom, is based upon three-dimensional imaging techniques such as magnetic resonance (MR) imaging and computed tomography (CT). The tomographic phantoms represent the human anatomy with a large number of voxels that are assigned tissue type and organ identity. To date, a total of around 30 tomographic phantoms including male and female adults, pediatric phantoms, and even a pregnant female, have been developed and utilized for realistic radiation dosimetry calculation. They are based on MRI/CT images or sectional color photos from patients, volunteers or cadavers. Several investigators have compared tomographic phantoms with stylized phantoms, and demonstrated the superiority of tomographic phantoms in terms of realistic anatomy and dosimetry calculation. This paper summarizes the history and current status of both stylized and tomographic phantoms, including Korean computational phantoms. Advantages, limitations, and future prospects are also discussed.

• 한국지리정보학회지
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• 제9권2호
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• pp.54-66
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• 2006

2005년 5월 산림청에서 전국 산지를 대상으로 산사태 위험지도를 제작하였다. 본 연구는 산사태 위험지도의 정확성 검증을 위하여 2005년 8월 2~3일 전북지역에서 발생된 산사태 지역을 대상으로 부경대학교 위성정보과학과 PE&RS Lab에서 개발한 PKNU 3호 시스템으로 촬영한 영상을 이용하여 산사태 발생 지역을 추출한 후 산사태 위험지도와 중첩해서 산사태 위험지도의 정확도를 검증하였다. 또한 ArcView 3.3의 산지 지형 분석과 하계망 분석을 통해 산사태 발생 지역의 고도, 경사도, 경사방향, 하천장, 유역면적에 대한 특성을 분석하였다. 그 결과 산사태 위험지도의 등급별 단위 %량의 조정이 필요하며 산사태 위험지도 작성에 기본이 되는 산사태 위험 판정표에 대한 수정 보완이 필요하다고 사료된다.

• 한국환경과학회지
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• 제17권9호
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• pp.981-992
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• 2008

This study examined how to produce new methods of copper (II) sulfate crystallization by using a small-scale chemistry tool such as small-scale reaction surface and petri dish. The making of copper(II) sulfate is included in the 5th grade elementary science textbooks. Various copper(II) compounds were reacted with a 2 M sulfuric acid solution. The result of this study is as follows: Seven small amounts of copper(II) compounds were reacted with a few drops of 2 M sulfuric acid solution at room temperature to make a copper(II) sulfate crystal of triclinic shape. Using the petri dish method, a copper(II) sulfate crystal could be identified within one hour of reacting copper(II) hydroxide, copper(II) carbonate, copper(II) nitrate, copper(II) perchlorate, cupric(II) formate from a few drops of 2 M sulfuric acid solution at room temperature. When using the lap top method for copper(II) perchlorate, cupric formate, a proper crystal could be identified within one hour as well. SSC methods were used for the first time to make a copper sulfate crystal via chemical reaction. We can make a copper(II) sulfate crystal using a simple method which is easier, safer and saves time in class. And since a small quantity of chemicals are being used in SSC chemical methods, waste is greatly reduced. This lessens the amount of environmental problems caused by the experiment. This can be helpful in preserving nature. In addition the cost of chemical and laboratory equipment is greatly reduced because it uses material that we find in our daily lives. There will be continued study of small-scale methods such as improvement of new programs, study and training of teachers, and securing SSC tools. I would like to suggest such as SSC methods are applicable in elementary School Science. I would like it to become a wide spread program.

• 천문학회지
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• 제38권4호
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• pp.437-443
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• 2005

The Solar Radio Burst Locator (SRBL) is a spectrometer that can observe solar microwave bursts over a wide band (0.1-18 GHz) as well as detect the burst locations without interferometry or mechanical scanning. Its prototype has been operated at Owens Valley Radio Observatory (OVRO) since 1998. In this study, we have evaluated the capability of the SRBL system in flux and radio burst location measurements. For this, we consider 130 microwave bursts from 2000 to 2002. The SRBL radio fluxes of 53 events were compared with the fluxes from USAF/RSTN and the burst locations of 25 events were compared with the optical flare locations. From this study, we found: (1) there is a relatively good correlation (r = 0.9) between SRBL flux and RSTN flux; (2) the mean location error is about 8.4 arcmin and the location error (4.7 arcmin) of single source events is much smaller than that (14.9 arcmin) of multiple source events; (3) the minimum location error usually occurred just after the starting time of burst, mostly within 10 seconds; (4) there is a possible anti-correlation (r = -0.4) between the pointing error of SRBL antenna and the location error. The anti-correlation becomes more evident (r=-0.9) for 6 strong single source events associated with X-class flares. Our results show that the flux measurement of SRBL is consistent with that of RSTN, and the mean location error of SRBL is estimated to be about 5 arcmin for single source events.

• 대한의생명과학회지
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• 제13권1호
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• pp.47-53
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• 2007

Due to wide abuse of antibiotics both in human and livestock use, the advent and spread of multidrug resistant (MDR) pathogens becomes a serious health problem all over the world. Since the development of new antibiotics is at a standstill in pharmaceutical industry, the choice of therapeutic antibiotics is getting narrower. In this study, in an effort to search new antibiotics, the antimicrobial activity of Paenibacillus DY1 isolated from Korean soil was characterized on its growth inhibition spectrum against various health threatening MDR strains, with its stability and chemical structure. Extracellular culture filtrate of Paenibacillus DY1 effectively inhibits the growth of all the tested MDR enteropathogenic Eshcherichia coli, enterohemolytic E. coli, and enterotoxigenic E. coli strains, at a similar level to that on the nonresistant control E. coli strains. It showed significant growth inhibition effect against the causative agents of class one legal communicable disease, MDR Salmonella typhi, MDR Salmonella paratyphi A, food poisoning bacteria, MDR Salmonella typhimurium, and other MDR Salmonella spp. The growth of all of 10 different MDR Shigella spp. strains and 6 different Vibrio spp. strains tested was also inhibited. The antimicrobial activity of Paenibacillus DY1 was well preserved after heat treatment, and was also stable in both alkaline and acidic environment. The antimicrobial activity was partially purified with Diaion HP20 column and TLC. By NMR study, the putative structure of the activity was postulated as an alkane having hydroxyl groups.

• BMB Reports
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• 제42권1호
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• pp.16-21
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• 2009

Three novel Class A genes that encode heat shock transcription factor (HSF) were cloned from Oryza Sativa L using a yeast hybrid method. The OsHSF7 gene was found to be rapidly expressed in high levels in response to temperature, which indicates that it may be involved in heat stress reception and response. Over-expression of OsHSF7 in transgenic Arabidopsis could not induced over the expression of most target heat stress-inducible genes of HSFs; however, the transcription of some HSF target genes was more abundant in transgenic plants following two hours of heat stress treatment. In addition, those transgenic plants also had a higher basal thermotolerance, but not acquired thermotolerance. Collectively, the results of this study indicate that OsHSF7 might play an important role in the response to high temperature. Specifically, these findings indicate that OsHSF7 may be useful in the production of transgenic monocots that can over-express protective genes such as HSPs in response to heat stress, which will enable such plants to tolerate high temperatures.

• Molecules and Cells
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• 제37권2호
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• pp.161-171
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• 2014

In several lysosomal storage disorders, including Niemann-Pick disease Type C (NP-C), sphingolipids, including glycosphingolipids, particularly gangliosides, are the predominant storage materials in the brain, raising the possibility that accumulation of these lipids may be involved in the NP-C neurodegenerative process. However, correlation of these accumulations and NP-C neuropathology has not been fully characterized. Here we derived NP-C mice with complete and partial deletion of the Siat9 (encoding GM3 synthase) gene in order to investigate the role of ganglioside in NP-C pathogenesis. According to our results, NP-C mice with homozygotic deletion of GM3 synthase exhibited an enhanced neuropathological phenotype and died significantly earlier than NP-C mice. Notably, in contrast to complete depletion, NP-C mice with partial deletion of the GM3 synthase gene showed ameliorated NP-C neuropathology, including motor disability, demyelination, and abnormal accumulation of cholesterol and sphingolipids. These findings indicate the crucial role of GM3 synthesis in the NP-C phenotype and progression of CNS pathologic abnormality, suggesting that well-controlled inhibition of GM3 synthesis could be used as a therapeutic strategy.

• 한국조리학회지
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• 제10권3호
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• pp.51-64
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• 2004

This study was designed to examine the educational conditions of a foodstylist and student satisfaction with the training. It looked at the educational conditions of the food-stylist department, one of relatively newly-emerging departments, and examined student satisfaction by each factor in order to have specific problem in the educational conditions. The purpose of this study was to maintain higher student satisfaction and to have competitive advantage over the other related departments. For the general information of respondents, most respondents said the monthly average income to be approximately 1.5 times higher than that of common workers in the country, showing that the food-stylist department is preferred by the class with economic ability. For admission motives, respondents used the information via mass communication when they choose it For general educational conditions, students have most satisfaction with the originality of department, at the score of 3.79, following by laboratory and vision in the order. The least satisfaction was in career guidance, reflecting that they have vague ideas where to work. For the theoretical course of study, it was found that ‘Introduction to Table Coordination’ had the highest satisfaction, 3.82, following by ‘Coloring’ and ‘Understanding on Oriental and Occidental Tablewares’ in the order. ‘Food and Beverage Service’ and ‘Public Health’ were found to have the least satisfaction, showing that those subjects do not cause any interest in terms of their property. For the satisfaction of practical training course, it was found ‘Cocktail Practices’, ‘Bakery and Confectionary Practices’, and ‘Understanding of Wines’ in the order, showing that practical subjects with high visuality have higher satisfaction. Further studies are necessary to examine the satisfaction with subjects from various classes, together with many course of study, and to develop statistical data that help us understand the needs of students more correctly for application.