• Journal of Science Education
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• v.39 no.1
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• pp.1-14
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• 2015

This study aims to find out the effect of pre-service elementary teachers' experiences of inquiry performance in the life on their images about a scientist and science teaching anxiety. Thus, this study selected 162 college juniors who completed 'Science Teaching Material Research & Teaching Methods' for one semester at a university of education. Groups of 3 to 4 pre-service teachers were made to select one of the 10 experimental topics and inquired it for themselves, and make a presentation about the results before the other pre-service teachers. The results of this study can be summarized as below. Firstly, as a result of investigating pre-service elementary teachers' opinions about the characteristics of scientists, it was found that they thought of scientists prudent, intellectually superior, creative, and hard-working, but did not consider them artistic or religious, no matter if they had inquiry performance experiences. Especially, to such characteristics as intellectually-superior, creative, open-minded, outgoing and responsible, they showed more positive responses after inquiry performances. Besides, in scientists' activity types shown in pre-service elementary teachers' drawing pictures of a scientist, 'Experimental Activity' increased most after their inquiry performances, and in the place of activity, 'Laboratory' was most since it was related to activity types. Secondly, as a result of examining per-service elementary teachers' science teaching anxiety before and after inquiry performances, it was found that they showed statistically significant differences in all the domains, scientific knowledge, inquiry class preparation and inquiry class management. This finding could be interpreted as their inquiry experiences had positive effect on their having confidence in teaching science.

• Journal of Veterinary Clinics
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• v.33 no.2
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• pp.102-106
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• 2016

More than 20 years after the first report of porcine reproductive and respiratory syndrome virus (PRRSV) in Korea, the disease is still having major impact on domestic pig health and relevant industries. Although ELISA tests are commonly used by veterinarians to guide herd management, data on diagnostic performance of the test in field settings are very limited. The objective of this study was to evaluate two commercially available PRRSV ELISA (IDEXX PRRS X3 ELISA and Bionote PRRSV ELISA 4.0) to detect antibodies against PRRSV on serum samples. To this end, a total of 1,108 sera were recruited from 35 swine farms located in Gyeonggi province and tested at the Gyeonggi Province Veterinary Service Center. All tests were performed according to the manufacturer's instructions, by laboratory technicians who routinely perform PRRS testing on blood samples. Samples were collected from two sources of swine populations with different PRRS prevalence; 60 samples (5.4%) were originated from breeding farms and the remaining 1,048 samples (94.6%) were from farrow-to-finish farms. We applied Bayesian latent class model (LCM) for two-tests in the two-population when the accuracy of the gold standard is not available. The model estimated that Bionote ELISA was a bit more specific but slightly less sensitive. The estimated sensitivity and specificity of the IDEXX ELISA were 99.8% (95% CI 98.1-100%) and 86.4% (95% CI 81.4-96.5%), respectively. Sensitivity, specificity, positive predictive value and negative predictive value for Bionote kit were 98.7% (95% CI 92.8-100%), 89.8% (95% CI 86.2-93.1%), 93.8% (95% CI 91.5-96.0%), and 97.8% (95% CI 87.1-100%), respectively. Based on the Bayesian 95% credible intervals, the sensitivity and specificity of the two ELISAs were not significantly different each other when assuming that two kits were imperfect, indicating that two kits performed equally well in terms of sensitivity and specificity in our filed setting.

• Journal of The Korean Astronomical Society
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• v.54 no.3
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• pp.89-102
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• 2021

Even in an era where 8-meter class telescopes are common, small telescopes are considered very valuable research facilities since they are available for rapid follow-up or long term monitoring observations. To maximize the usefulness of small telescopes in Korea, we established the SomangNet, a network of 0.4-1.0 m class optical telescopes operated by Korean institutions, in 2020. Here, we give an overview of the project, describing the current participating telescopes, its scientific scope and operation mode, and the prospects for future activities. SomangNet currently includes 10 telescopes that are located in Australia, USA, and Chile as well as in Korea. The operation of many of these telescopes currently relies on operators, and we plan to upgrade them for remote or robotic operation. The latest SomangNet science projects include monitoring and follow-up observational studies of galaxies, supernovae, active galactic nuclei, symbiotic stars, solar system objects, neutrino/gravitational-wave sources, and exoplanets.

• Microbiology and Biotechnology Letters
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• v.51 no.4
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• pp.517-525
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• 2023

The emergence and spread of multidrug-resistant Pseudomonas aeruginosa (MRPA) have become a serious problem worldwide. The involvement of metallo-β-lactamases (MBLs) in inducing carbapenem resistance is particularly acute. However, unlike other members of the Enterobacteriaceae genus, new clones of P. aeruginosa are constantly emerging and rapidly replacing previously prevalent dominant clones. Therefore, this study aimed to perform antimicrobial resistance gene analysis, integron gene cassette analysis using DNA sequencing, and plasmid transfer analysis by conjugation to investigate the antimicrobial resistance dynamics of 18 P. aeruginosa strains isolated from various medical samples at a general hospital in Busan from September 2017 to September 2019. All 18 strains showed extensively drug-resistant (XDR) phenotype and were resistant to most antibiotics, except colistin (100%) but were susceptible to aztreonam (22.2%) and ceftazidime (16.6%). Approximately 66.7% of the strains had Class 1 integrons showing various antimicrobial resistances. Notably, IMP-6 ST235 (66.7%), VIM-2 ST357 (16.7%), and IMP-1 ST446(16.7%) were identified. The identification of IMP-1-producing ST446, previously unreported in Korea, is noteworthy considering the emergence and prevalence of another MRPA high-risk clone.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.42 no.5
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• pp.430-436
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• 2014

The mission objective of STEP Cube Lab. (Cube Laboratory for Space Technology Experimental Project) classified as a pico-class satellite is to verify the technical effectiveness of payloads such as variable emittance radiator, SMA washer, oscillating heat pipe and MEMS based solid propellant thruster researched at domestic universities. In addition, the MEMS concentrating photovoltaic power system and the non-explosive holding and separation mechanism with the advantages of high constraint force and low shock level will be developed as the primary payloads for on-orbit verification. In this study, the feasibility of the mission actualization has been confirmed by the preliminary system design.

• International Journal of Industrial Entomology and Biomaterials
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• v.18 no.1
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• pp.18-27
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• 2009

B. thuringiensis strain LY-99 belonging to subsp. alesti (H3a3c), was isolated from Chinese tobacco warehouse and showed significantly high toxicity to Plutella xylostella. For the identification of the cry1-type genes from B. thuringiensis LY-99, an extended multiplex PCRrestriction fragment length polymorphism (PCRRFLP) method was established by using two pairs of universal primers based on the conserved regions of the cry1-type genes to amplify around 2.4 kb cry1-type gene fragments. Then the DNA fragment was cloned into pGEM-T Easy vector and digested with EcoRI and EcoRV enzymes. Through this method, a known cry1-type gene was successfully identified from the reference strain, B. thuringiensis subsp. alesti. In addition, the RFLP patterns revealed that B. thuringiensis LY-99 included a novel cry1A-type gene in addition to cry1Aa, cry1Ac, cry1Be and cry1Ea genes. The novel cry1A-type gene was designated cry1Ah2 (Genbank accession No DQ269474). An inverse PCR method was used to amplify the flank regions of cry1Ah2 gene. Finally, 3143 bp HindIII fragment from B. thuringiensis LY-99 plasmid DNA including 5' region and partial ORF was amplified, and sequence analysis revealed that cry1Ah2 gene from LY-99 showed 89.31% of maximum sequence similarity with cry1Ac1 crystal protein gene. In addition, the deduced amino acid sequence of Cry1Ah2 protein shared 87.80% of maximum identity with that of Cry1Ac2. This protein therefore belongs to a new class of B. thuringiensis crystal proteins.

• Molecules and Cells
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• v.43 no.10
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• pp.870-879
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• 2020

Dendrites require precise and timely delivery of protein substrates to distal areas to ensure the correct morphology and function of neurons. Many of these protein substrates are supplied in the form of ribonucleoprotein (RNP) complex consisting of RNA-binding proteins (RBPs) and mRNAs, which are subsequently translated in distal dendritic areas. It remains elusive, however, whether key RBPs supply mRNA according to local demands individually or in a coordinated manner. In this study, we investigated how Drosophila sensory neurons respond to the dysregulation of a disease-associated RBP, Ataxin-2 (ATX2), which leads to dendritic defects. We found that ATX2 plays a crucial role in spacing dendritic branches for the optimal dendritic receptive fields in Drosophila class IV dendritic arborization (C4da) neurons, where both expression level and subcellular location of ATX2 contribute significantly to this effect. We showed that translational upregulation through the expression of eukaryotic translation initiation factor 4E (eIF4E) further enhanced the ATX2-induced dendritic phenotypes. Additionally, we found that the expression level of another disease-associated RBP, fragile X mental retardation protein (FMRP), decreased in both cell bodies and dendrites when neurons were faced with aberrant upregulation of ATX2. Finally, we revealed that the PAM2 motif of ATX2, which mediates its interaction with poly(A)-binding protein (PABP), is potentially necessary for the decrease of FMRP in certain neuronal stress conditions. Collectively, our data suggest that dysregulation of RBPs triggers a compensatory regulation of other functionally-overlapping RBPs to minimize RBP dysregulation-associated aberrations that hinder neuronal homeostasis in dendrites.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.1621-1621
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• 2001

The quality of sugarcane grown on the NE Australian tropical coast ($16^{\circ}$15'- $18^{\circ}$15' S Lat.) has declined markedly in the past seven years. This has been linked to dilution of mill-supply cane with increasing levels of non mature-stalk material consisting of leaves and sucker culms. The prime research objective was to examine the transition from the pre-harvest, in-field crop to harvested material sent for processing, in terms of quality and crop fraction proportions. A secondary objective was to quantify the effects of preharvest-season crop habit and culm condition on crop quality. Ten quadrat samples from each of 54 random crop sites (17 in 1999 and 37 in 2000), covering a wide range of variables (cultivar, crop class, and edaphic, topographic, climatic, and temporal factors) were collected immediately before harvest. Samples were partitioned into four fractions:- sound and unsound mature stalks (culms), sucker culms, and extraneous matter (leaves). Material harvested from each site was sampled and partitioned into four fractions:- sound and unsound billets (culm pieces), culm-spindle pieces, and leaf. In 2000, before harvest, 14 additional sites were sampled monthly, on three occasions, from March - June. Erect and non-erect culms were divided into sound and unsound classes. All samples were disintegrated and presented to a remote reflectance module of a scanning spectrophotometer using the BSES large cassette module. Near infra-red spectroscopic (NIS) analyses were developed for the rapid determination of quality components (Brix, commercial cane sugar (CCS), fibre, moisture, and polariscope reading). Calibrations for three material groups (culm (n = 639), non-culm (n = 496), and combined) were developed for all components using the 1999 data set. Two sub-sets (n = 178, and 190) of about 10% of the preharvest-season and harvest populations scanned in 2000 also were subjected to full routine laboratory analyses. The 1999 combined calibrations were excellent, but the culm calibrations produced consistently lower standard errors. Non-culm calibrations were marginally better than the combined for only CCS and pol. reading. Analysis of the 2000 culm data with calibrations using all 1999 and 2000 culm data resulted in better predictions relative to the 1999 culm calibrations. This also was true for the combined calibrations. Assessment of quality components in pre- and post-harvest sugarcane using NIS calibrations was more cost effective than using routine laboratory techniques. Outcomes from this NIS-facilitated research will have important economic consequences for the Australian sugarcane industry. Potential CCS present in mature culms is being discounted by dilution with leaves and sucker culms, threatening farm viability. The results question the efficacy of current harvesting technology. The CCS of harvested cane is improved only marginally over that of the in-field crop. Current harvesting technology requires either supplementary, innovative pre-mill processing or a design revolution to improve mill-supply cane quality, and therefore whole of industry economics. NIS-facilitated analyses, before the harvest season, highlighted the benefits of growing erect, sound crops. Loss of CCS then, can be minimized only by a combination of crop improvement and agronomic solutions, applied as part of sound on-farm management.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.18 no.3
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• pp.122-130
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• 2013

To examine floc characteristics of cohesive bed sediment of the Yeongsan River estuary, a floc camera system has been developed and utilized to observe flocs under varying conditions. In order to validate the floc camera system, sand particles were passed through 88-125 and $63-88{\mu}m$ sieves and observed within the laboratory. Mean grain size and settling velocities were found to be 102 and $56.2{\mu}m$ and 6.7 and 5.9 mm/s, respectively. Artifacts of particles estimated outside of the sieve range are attributed to being imaged out of the depth of focus. However, as mean grain size and settling velocity of each size class were within the confidence interval, the floc camera system was confidently used to examine cohesive bed sediments of Yeongsan River estuary. The bed sediment sample was prepared with a concentration of 0.1 g/L in 0 psu deionized water. The mean grain size, settling velocity and fractal dimension of flocs were $40.6{\pm}0.66{\mu}m$, 14 mm/s, and 2.86, respectively. Experiments were also conducted using different salinities (10 and 34 psu) and sediment concentrations (0.1 and 0.3 g/L). Despite changing these parameters, the mean observed grain size and settling velocities were found to be the same within the error range of the system. The relatively higher values of settling velocity and fractal dimension are considered a result of the sediment containing relatively small concentrations of organic matter. Moreover, consistent floc size over various grain sizes and concentrations may be the result of insufficient turbulence to aggregate flocs.

• Journal of Technologic Dentistry
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• v.28 no.2
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• pp.331-346
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• 2006

Free-end partial dentures, which are supported by teeth surrounded by dental root membranes and elastic mucous membrane tissues, may cause stress to the abutment teeth due to external force imposed on the denture base, increase the mobility of the abutment teeth, and bring about a change in the periodontal tissue. General retainers used in partial dentures are categorized into clasp, attachment, and Konus crown. Stress imposed on the abutment teeth and mobility of the denture base have relations with the lifetime of a crown and abutment teeth, and have direct relations with the chewing ability. Thus, a need arises to make a comparative analysis of stress of the three direct retainers on the abutment teeth and interpret the mobility of the denture base. This study designed three kinds of removable partial dentures (one kind of attachment partial denture, one kind of Konus crown partial denture, and one kind of clasp partial denture), and fabricated Dentiforms of bilateral partial dentures (Kennedy Class I) with lower left 1st premolar and lower right 1st and 2nd premolars being as the abutment teeth. A strain gauge was installed in the mesial and distal surface of the lowerr left 1st premolar (No. 34) of the fabricated dentiform and in the lower part of the denture base, and installed were a clasp partial denture, an attachment partial denture, and a Konus crown partial denture. Then, the vertical static load of 5kgf and 7.5kgf at the occlusion surface of the lower left No. 6 molar was generated for a total of 20 frequencies of load each using a push-full gauge, and thus a change in the output of the strain gauge was measured. With the respective application of Konus crown, attachment and RPI clasp in the free-end partial denture, surveyed was the distribution of stress imposed on the abutment teeth and the denture base according to the location of occlusion force load so as to come up with the following results. 1. Konus crown and attachment partial dentures generated much stress, and more stress on the abutment teeth than RPI clasp dentures did. Attachment dentures tended to further intensify stress on the abutment teeth than Konus crown dentures did. 2. Attachment dentures and Konus dentures imposed less stress on the denture base than RPI clasp dentures did. There was no stress difference between Attachment and Konus crown dentures. 3. Dentures that were designed with the application of retainers using sturdy linkage methods tended to be less mobile.