• Korean Journal of Clinical Laboratory Science
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• v.49 no.4
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• pp.489-494
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• 2017

Human anatomy courses are one of the most fundamental subjects taught to students in any health-related science field. To improve the quality and to enlarge the scope of human anatomy courses, 130 students (Biomedical Laboratory Science, Physical Therapy, and Occupational Therapy) were given a questionnaire survey. The students were asked question regarding their anatomy course and cadaver dissection class. Specific questions regarding knowledge acquisition before and after the course was queried, in addition to their views on the necessity of cadaver dissection. The survey results indicated that students of all three departments were satisfied with the anatomy course, with no statistical difference in the students' sex or department affiliation. Although there was no difference between the three majors with respect to understanding the course materials, Biomedical Laboratory Science students showed higher satisfaction in this course (p<0.05). Most students, regardless of sex and high school education, stated that cadaver dissection is an important part of the anatomy course. In conclusion, anatomy courses should implement cadaver dissection and actual practice rather than textbook-related studies alone.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.7
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• pp.957-966
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• 2017

Objective: To understand the dynamic structure, function, and influence on nutrient metabolism in hosts, it was crucial to assess the genetic potential of gut microbial community in yaks of different ages. Methods: The denaturing gradient gel electrophoresis (DGGE) profiles and Illumina-based metagenomic sequencing on colon contents of 15 semi-domestic yaks were investigated. Unweighted pairwise grouping method with mathematical averages (UPGMA) clustering and principal component analysis (PCA) were used to analyze the DGGE fingerprint. The Illumina sequences were assembled, predicted to genes and functionally annotated, and then classified by querying protein sequences of the genes against the Kyoto encyclopedia of genes and genomes (KEGG) database. Results: Metagenomic sequencing showed that more than 85% of ribosomal RNA (rRNA) gene sequences belonged to the phylum Firmicutes and Bacteroidetes, indicating that the family Ruminococcaceae (46.5%), Rikenellaceae (11.3%), Lachnospiraceae (10.0%), and Bacteroidaceae (6.3%) were dominant gut microbes. Over 50% of non-rRNA gene sequences represented the metabolic pathways of amino acids (14.4%), proteins (12.3%), sugars (11.9%), nucleotides (6.8%), lipids (1.7%), xenobiotics (1.4%), coenzymes, and vitamins (3.6%). Gene functional classification showed that most of enzyme-coding genes were related to cellulose digestion and amino acids metabolic pathways. Conclusion: Yaks' age had a substantial effect on gut microbial composition. Comparative metagenomics of gut microbiota in 0.5-, 1.5-, and 2.5-year-old yaks revealed that the abundance of the class Clostridia, Bacteroidia, and Lentisphaeria, as well as the phylum Firmicutes, Bacteroidetes, Lentisphaerae, Tenericutes, and Cyanobacteria, varied more greatly during yaks' growth, especially in young animals (0.5 and 1.5 years old). Gut microbes, including Bacteroides, Clostridium, and Lentisphaeria, make a contribution to the energy metabolism and synthesis of amino acid, which are essential to the normal growth of yaks.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.10
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• pp.139-146
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• 2016

The purpose of this study is to investigate the necessity of safety awareness and education regarding the use of laboratories, and to foster safety awareness during intramural and extracurricular activities in high schools. The study conducted a questionnaire to 290 eleventh grade students of three high schools located in Seoul. The collected data were statistically analyzed. The result illustrates that 64.8% of students have experienced safety education during a regular class, 6.6% have experienced accidents, and 68.0% have experienced safety education during field activities. The result concludes that there is a lack of prior safety education as well as overall low awareness on the necessity of safety education. In particular, students who have experienced safety education recognizes the necessity of emergency education regarding laboratory accident more than those who did not receive such education. Thus, a systematic management of laboratory safety education and multilateral management measures for students' safety are required. There is a relatively high correlation between safety intention of R&D and Education factors and Community and Laboratory Safety Education. As much as there is a high safety demand regarding the laboratory instruments and experiment, it shows high intention of safety lab operation as well as count measurement of lab accident. This study recommends developing a laboratory safety education program and management methods appropriate for high school students.

• Animal Bioscience
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• v.36 no.10
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• pp.1488-1498
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• 2023

Objective: α_S1-Casein is more closely associated with milk allergic reaction than other milk protein components. microRNA (miRNA) is a class of small non-coding RNAs that modulate multiple biological progresses by the target gene. However, the post-transcriptional regulation of α_S1-casein expression by miRNA in ruminants remains unclear. This study aims to explore the regulatory roles of miR-380-3p on α_S1-casein synthesis in goat mammary epithelial cells (GMEC). Methods: α_S1-Casein gene and miR-380-3p expression was measured in dairy goat mammary gland by quantitative real-time polymerase chain reaction (qRT-PCR). miR-380-3p overexpression and knockdown were performed by miR-380-3p mimic or inhibitor in GMEC. The effect of miR-380-3p on α_S1-casein synthesis was detected by qRT-PCR, western blot, luciferase and chromatin immunoprecipitation assays in GMEC. Results: Compared with middle-lactation period, α_S1-casein gene expression is increased, while miR-380-3p expression is decreased during peak-lactation of dairy goats. miR-380-3p reduces α_S1-casein abundance by targeting the 3'-untranslated region (3'UTR) of α_S1-casein mRNA in GMEC. miR-380-3p enhances β-casein expression and signal transducer and activator of transcription 5a (STAT5a) activity. Moreover, miR-380-3p promotes β-casein abundance through target gene α_S1-casein, and activates β-casein transcription by enhancing the binding of STAT5 to β-casein gene promoter region. Conclusion: miR-380-3p decreases α_S1-casein expression and increases β-casein expression by targeting α_S1-casein in GMEC, which supplies a novel strategy for reducing milk allergic potential and building up milk quality in ruminants.

• Computers and Concrete
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• v.34 no.4
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• pp.489-501
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• 2024

Geopolymers are part of a class of materials characterized by properties combining polymers, ceramics, and cement. These include exceptionally high thermal and chemical stability, excellent mechanical strength and durability in aggressive environments. This work deals with the synthesis, characterization, and sustainability evaluation of GP_GBFS-MK geopolymers by alkaline activation of a granulated blast furnace slag-metakaolin mixture. In the first step, elemental and oxide analyses by XRF and EDS showed that the main constituents of GP_GBFS-MK geopolymers are silicon, sodium, and aluminium oxides. The structural analyses by XRD and FTIR confirmed that the geopolymerization for GP_GBFS-MK geopolymers did occur, accompanied by the formation of disordered networks from the blends and a modification to the microstructure by the geopolymerization process. Similarly, the microstructural study made by SEM showed that the GP_GBFS-MK geopolymers are constituted by aluminosilicates in the form of dense clusters on which are adsorbed particles of unreacted GBFS in the form of spheroids and white residues of the alkaline activating solution. In addition, the study of the sustainability evaluation of GP_GBFS-MK geopolymers showed that the water absorption of geopolymeric materials is lower than that of OPC cement. As for the elevated temperature resistance, the analyses indicated an excellent elevated temperature resistance of GP_GBFS-MK. In the same way, the study of the resistance to chemical aggressions showed that the GP_GBFS-MK geopolymeric materials are unattackable, contrary to the OPC cement-based materials which are strongly altered.

• Analytical Science and Technology
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• v.24 no.6
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• pp.435-442
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• 2011

Polybrominated diphenyl ethers (PBDEs) are a class of brominated flame retardants (BFRs) which have taken much interest recently due to their potential hazardous effects to human body and ecosystem. Many countries and European community prohibits the usage of certain BFRs in electronics and electronic devices (e.g. RoHS). In this perspectives, Korea Research Institute of Standards and Science (KRISS) has designed and practiced proficiency testing programs based on the ISO/IEC 17043 in order to assist laboratory accreditation activities. The programs for interlaboratory comparisons include congeners of PBDE (PBDE-154, 183, 206, 209) in high impact polystyrene (HIPS). A sample bottle that contains 10 g granular HIPS was distributed to 35 participating laboratories and the test results were calculated by the statistical procedure using z-scores to evaluate performance of each laboratory. The results and the laboratory's performance were discussed.

• BMB Reports
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• v.34 no.2
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• pp.176-181
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• 2001

Using a retrovirus, foreign genes can be introduced into mammalian cells. The purpose of this study is to produce a retrovirus that can make the infected cells express two genes; the human multidrug resistance gene (MDR1) and the HLA-B7 gene, which is one of the major human histocompatibility complex (MHC) class I genes. For the expression of these genes, the internal ribosome entry site (IRES) was used, which was derived from the encephalomyocarditis (EMC) virus. In order to produce retroviruses, a retroviral vector was transfected into a packaging cell line and the transfected cells were treated with vincristine, which is an anti-cancer drug and a substrate for the MDRI gene product. This study revealed that two genes were incorporated into chromosomes of selected cells and expressed in the same cells. The production of the retrovirus was confirmed by the reverse transcription (RT)-PCR of the viral RNA. The retrovirus that was produced infected mouse fibroblast cells as well as the human U937. This study showed that packaging cells produced the retroviruses, which can infect the target cells. Once the conditions for the high infectivity of retrovirus into human cells are optimized, thus virus will be used to infect hematopoietic stem cells to co-express MDRl and HLA-B7 genes, and develop the lymphocytes that can be used for the immnogene therapy.

• Journal of the Korean earth science society
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• v.31 no.6
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• pp.637-646
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• 2010

The purpose of this study was to investigate the effects of Microcomputer Based Laboratory (MBL) on the middle school students' graphing skill and science process skill in Earth Science classes. MBL teaching materials for inquiry-based instructions were developed to teach five experimental topics related to the middle school 'Earth Science.' Thirty three middles school students participated in this study, and the pre- and post-tests were conducted. Results indicated as follow: First, MBL classes were effective in developing the graphing skill of middle school students. They were efficient both in graph construction and graph interpretation, especially in data transformation. Second, MBL classes were effective in helping participants to use science process skills, especially, in the subordinate areas including formulating hypotheses, making operational definition, graphing, and interpreting data.

• Structural Engineering and Mechanics
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• v.68 no.4
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• pp.385-398
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• 2018

In the present paper, we offer a new flat shell finite element. It is the result of the combination of a membrane element and a bending element, both based on the strain-based formulation. It is known that $C^{\circ}$ plane membrane elements provide poor deflection and stress for problems where bending is dominant. In addition, they encounter continuity and compliance problems when they connect to C1 class plate elements. The reach of the present work is to surmount these problems when a membrane element is coupled with a thin plate element in order to construct a shell element. The membrane element used is a triangular element with four nodes, three nodes at the vertices of the triangle and the fourth one at its barycenter. Each node has three degrees of freedom, two translations and one rotation around the normal. The coefficients related to the degrees of freedom at the internal node are subsequently removed from the element stiffness matrix by using the static condensation technique. The interpolation functions of strain, displacements and stresses fields are developed from equilibrium conditions. The plate element used for the construction of the present shell element is a triangular four-node thin plate element based on Kirchhoff plate theory, the strain approach, the four fictitious node, the static condensation and the analytic integration. The shell element result of this combination is robust, competitive and efficient.

• Journal of Communications and Networks
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• v.8 no.2
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• pp.234-240
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• 2006

We study the dynamic burst assembly based on traffic prediction and offset and delay differentiation in optical burst switching network. To improve existing burst assembly mechanism and build an adaptive flexible optical burst switching network, an approach called quality of service (QoS) based adaptive dynamic assembly (QADA) is proposed in this paper. QADA method takes into account current arrival traffic in prediction time adequately and performs adaptive dynamic assembly in limited burst assembly time (BAT) range. By the simulation of burst length error, the QADA method is proved better than the existing method and can achieve the small enough predictive error for real scenarios. Then the different dynamic ranges of BAT for four traffic classes are introduced to make delay differentiation. According to the limitation of BAT range, the burst assembly is classified into one-dimension limit and two-dimension limit. We draw a comparison between one-dimension and two-dimension limit with different prediction time under QoS based offset time and find that the one-dimensional approach offers better network performance, while the two-dimensional approach provides strict inter-class differentiation. Furthermore, the final simulation results in our network condition show that QADA can execute adaptive flexible burst assembly with dynamic BAT and achieve a latency reduction, delay fairness, and offset time QoS guarantee for different traffic classes.