• Title/Summary/Keyword: Laboratory class

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Analysis of Class 1 Integrons in Imipenem-resistant Pseudomonas aeruginosa

  • Sung, Ji Youn
    • Korean Journal of Clinical Laboratory Science
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    • v.43 no.2
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    • pp.68-74
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    • 2011
  • Pseudomonas aeruginosa is an aerobic, Gram-negative, glucose-nonfermenting bacterium, which has emerged as a serious opportunistic pathogen. Recently, outbreaks of carbapenem resistant P. aeruginosa give rise to significant therapeutic challenges for treating nosocomial infections. The genes of metallo-${\beta}$-lactamase (MBL), a powerful carbapenemase, are carried as a part of the mobile gene cassettes inserted into integrons playing an important role in rapid dissemination of antibiotic resistance genes among bacterial isolates. In this study, we investigated the prevalence of integron in imipenem resistant P. aeruginosa isolates. A total of 61 consecutive, non-duplicate, and imipenem resistant P. aeruginosa strains were isolated from a university hospital in the Chungcheong province of Korea. We employed repetitive extragenic palindromic sequence-based PCR (rep-PCR) method for the selection of clonally different P. aerusinosa strains. PCR and DNA sequencing were conducted for the detection of integrons. Twenty-one clonally different P. aeruginosa strains were isolated. Only one (P28) of the strains harbored $bla_{VIM-2}$ that was found as gene cassettes in class 1 integrons. Four of 21 carbapenem resistant P. aeruginosa strains harbored class 1 integron containing aminoglycoside resistance determinant. All of the integrons detected in the study contained more than one resistance gene cassette, which can mediate resistance to multiple antibiotics. To prevent further spreading of the multi-drug resistant P. aeruginosa, conseguent monitoring and clinical polices are required.

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Development of kW Class SOFC Systems for Combined Heat and Power Units at KEPRI

  • Lee, Tae-Hee;Choi, Jin-Hyeok;Park, Tae-Sung;Yoo, Keun-Bae;Yoo, Young-Sung
    • Journal of the Korean Ceramic Society
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    • v.45 no.12
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    • pp.772-776
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    • 2008
  • The Korea Electric Power Research Institute (KEPRI) has been developing planar solid oxide fuel cells (SOFCs) and power systems for combined heat and power (CHP) units. The R&D work includes solid oxide fuel cell (SOFC) materials investigation, design and fabrication of single cells and stacks, and kW class SOFC CHP system development. Anode supported cells composed of Ni-YSZ/FL/YSZ/LSCF were enlarged up to $15{\times}15\;cm^2$ and stacks were manufactured using $10{\times}10\;cm^2$ cells and metallic interconnects such as ferritic stainless steel. The first-generation system had a 37-cell stack and an autothermal reformer for use with city gas. The system showed maximum stack power of about $1.3\;kW_{e,DC}$ and was able to recover heat of $0.57{\sim}1.2\;kW_{th}$ depending on loaded current by making hot water. The second-generation system was composed of an improved 48-cell stack and a prereformer (or steam reformer). The thermal management subsystem design including heat exchangers and insulators was also improved. The second-generation system was successfully operated without any external heat source. Under self-sustainable operation conditions, the stack power was about $1.3\;kW_{e,DC}$ with hydrogen and $1.2\;kW_{e,DC}$ with city. The system also recuperated heat of about $1.1\;kW_{th}$ by making hot water. Recently KEPRI manufactured a 2kW class SOFC stack and a system by scaling up the second-generation 1kW system and will develop a 5kW class CHP system by 2010.

Distribution and Characterization of Integrons in Enterobacteriaceae Isolates from Chickens in Korea

  • Sung, Ji Youn;Oh, Ji-Eun
    • Journal of Microbiology and Biotechnology
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    • v.24 no.7
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    • pp.1008-1013
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    • 2014
  • The use of antimicrobial agents for additives or therapeutics is strongly associated with a prevalence of antimicrobial resistance in commensal Enterobacteriaceae. We aimed to characterize integrons in Enterobacteriaceae isolates obtained from chicken cecums in Korea. Moreover, the correlation between integron gene cassettes and antimicrobial resistance was also investigated. A total of 90 isolates the belonged to Enterobacteriaceae were recovered from chickens grown at Gyeongsang and Chungcheong provinces in Korea. Antimicrobial susceptibility tests were performed by the disk diffusion method. PCR and DNA sequencing were also performed to characterize the gene cassette arrays of the integrons. Of the 90 Enterobacteriaceae isolates tested, 39 (43.3%) and 10 (11.1%) isolates carried class 1 and 2 integrons, respectively. Whereas the class 2 integron did not contain gene cassettes, the class 1 integrons carried seven different gene cassette arrays. The class 1 integrons harbored genes encoding resistant determinants to aminoglycosides (aadA1, aadA2, and aadA5), trimethoprim (dfrA1, dfrA12, dfrA17, and dfrA32), lincosamides (linF), and erythromycin (ereA). Moreover, the presence of a class 1 integron was significantly related to a high resistance rate of antimicrobial agents, such as spectinomycin and trimethoprim. We confirmed that diverse class 1 integrons were widely distributed in Enterobacteriaceae isolates from chickens and directly contributed to the resistance to diverse antimicrobial agents in Korea.

Compact Wireless IPT System Using a Modified Voltage-fed Multi-resonant Class EF2 Inverter

  • Uddin, Mohammad Kamar;Mekhilef, Saad;Ramasamy, Gobbi
    • Journal of Power Electronics
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    • v.18 no.1
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    • pp.277-288
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    • 2018
  • Wireless inductive power transfer (IPT) technology is used in many applications today. A compact and high-frequency primary side inverter is one of the most important parts of a WPT system. In this study, a modified class EF-type voltage-fed multi-resonant inverter has been proposed for WPT application at a frequency range of 85-100 kHz. Instead of an infinite input choke inductor, a resonant inductor is used to reduce loss and power density. The peak voltage stress across the MOSFET has been reduced to almost 60% from a class-E inverter using a passive clamping circuit. A simple yet effective design procedure has been presented to calculate the various component values of the proposed inverter. The overall system is simulated using MATLAB/SimPowerSystem to verify the theoretical concepts. A 500-W prototype was built and tested to validate the simulated results. The inverter exhibited 90% efficiency at nearly perfect alignment condition, and efficiency reduced gradually with the misalignment of WPT coils. The proposed inverter maintains zero-voltage switching (ZVS) during considerable load changes and possesses all the inherent advantages of class E-type inverters.

Analysis of Descriptive Course Evaluation of University Chemistry Laboratory Class using Text Mining (텍스트 마이닝을 활용한 대학 화학 실험 수업의 서술형 강의 평가 내용 분석)

  • Yun, Jeonghyun;Park, Geumju
    • Journal of the Korean Chemical Society
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    • v.66 no.3
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    • pp.218-227
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    • 2022
  • The purpose of this study is to analyze the opinions of students by using the text mining to the good points and improvements among the descriptive course evaluation written by the students who participated in the university chemistry laboratory class, and to derive the improvement for the class. We analyzed the frequency of occurrence, co-occurrence and network of key words. As a result of the study, in the network of good points in the class, the most frequent mentions were made between class and professor, along with explanation, understanding, student, passion, fun, TA, experiment, help, etc. In the network of improvements in the class, the most frequent mentions were made between class and student, along with professor, content, explanation, exam, wish, experiment, understanding, difficult, thought, problem, etc. In other words, the students suggested the opinion that the contents of the class were well understood and that they felt fun and satisfied with the experimental process due to 'easy and detailed explanation' and 'TA's assistance' as good points of the class. On the other hand, the students suggested the negative opinions that the understanding and concentration in the class was decreased due to 'difficulty of content and exam', 'excessive assignments', and 'class environment' as improvements of the class.

Production of Chemistry Laboratory Class for Senior High School Freshmen

  • Yasuzawa, Mikito;Minagawa, Keiji;Kamitani, Sachiyo;Arai, Yuka;Konishi, Yuki;Nakanishi, Shinsuke;Oshima, Takuya;Yamaguchi, Junko;Ishii, Arisa
    • Journal of Engineering Education Research
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    • v.13 no.5
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    • pp.55-60
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    • 2010
  • Chemistry laboratory class was produced for senior high school freshmen with the cooperation of university staffs, high school teachers and the university students. Although the students who will take the lab class are senior high school freshmen, we decided to prepare four experiments that are simple and have highly visible reactions or transformation. That is, 1) Water purification, 2) Surface modification, 3) Briggs-Rauscher reaction, and 4) Polymer synthesis and characterization. After the safety guideline and experiment instructions by the faculty staffs, two teaching assistants (TAs) supervised each experiment. Since taking a direct part in it will provide stronger impact than only being one who is just watching the experiment, all experiments contained some process that the high school students must handle the reagents, tools or the equipment, by themselves. Although, the operation performed by the students was limited to a series of simple actions, the various unique phenomena presented by the experiments impressed the students. The lab class was fruitful not only for high school students, but also good for university students. The lab class provided good opportunity for them to improve the abilities to teach and guide someone.

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1kW class DC power supply by parallel operation of the flyback converter (Flyback Converter를 병렬운전한 1kW급 직류전원)

  • Kim, Sung-Chul;Lee, Gi-Sun;Nho, Jun-Tak;Ko, In-Soo
    • Proceedings of the KIEE Conference
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    • 1997.07f
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    • pp.2252-2254
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    • 1997
  • There are increasing demands on high accruacy DC power supplies for varous basic science reseach, such as accelerators and plasma applications, in Korea. In this paper, we present a study on 1kW class DC power supply using the parell operation method of the flyback converters. This DC power supply is equipped with a remote controller for computer interface. A Serial Communication Controller(SCC) is also developed for large scale scientific facilities which is capable of handling upto 32 power supplies simultaneously with one serial port in the host computer.

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In COVID-19, the Effect of Expected benefit of Time, Expected benefit of Learning, and Technology Familiarity in Online Class on Class Satisfaction (코로나 19로 인한 온라인 수업에서 시간적 기대 효익, 학습효과 기대 효익, 기술적 친숙도가 수업만족도에 미치는 영향)

  • Yu, Sang-Hui
    • Journal of the Korea Convergence Society
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    • v.12 no.11
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    • pp.257-263
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    • 2021
  • This study analyzed the factors affecting online class satisfaction and tried to use it as basic data for more effective class management. The survey was collected on 208 students majoring in dental laboratory technology in Jeonbuk and Chungbuk. The data were analyzed by reliability analysis, descriptive stastistics, compare means(t-test, one-way ANOVA), Pearson's correlation coefficient and stepwies multiple regression analysis(SPSS program). The analysis results showed that expected benefit of time was 3.87, expected benefit of learning was 3.30, technology familiarity was 3.40, and class satisfaction was 3.21. The most influential factor in class satisfaction was technology familiarity. In order to increase the online class satisfaction, it is necessary to build a learning environment to improve the ability to learning tools used in classes and technology familiarity with the online class system.