• 제목/요약/키워드: Laboratory class

검색결과 613건 처리시간 0.026초

Sequence analysis of the fusion protein gene of Newcastle disease virus isolated from breeder ducks in Korea

  • Han, Mi Na;Byeon, Hyeon Seop;Lee, Cho Yeon;Jo, Nam Sin;Lee, Jong Hwa;Jang, Rae Hoon;Kim, Chang Seop;Na, Ki Jeong
    • 한국동물위생학회지
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    • 제42권4호
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    • pp.245-250
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    • 2019
  • Newcastle disease (ND) is an infectious poultry disease that caused high mortality and reduced egg production. NDVs are regularly present in the domestic duck population. And ducks play a possible role in the maintenance and transmission of NDVs. While we were monitoring the Avian Influenza, NDVs were isolated from field samples by accident. So we analysed the biological and genetic characteristics of these viruses. Lentogenic NDVs were isolated from two farms among twenty breeder duck farms. The ages of ducks were 39 weeks old in the 'A' farm and 3~72 weeks old in the 'B' farm. And they were not inoculated with the NDVs vaccine. In the biological characteristics, the both viruses which separated from the farm 'A' and 'B' were thermostable. The amino acid sequence of a site from 112 to 119 in the fusion (F) protein was 'GKQGRLIG' which has monobasic motif in the samples of both farms. And this means the separated NDVs are lentogenic. Phylogenetic analysis was performed by entire nucleotide sequence of F protein. The virus strains from the A farm (MN095239) and the B farm (MN095240) belonged to class II genotype I. Using the analysis of whole F protein nucleic acid sequence, the MN095239 (GenBank) had homology with Ulster strain about 99.95% and the MN095239 (GenBank) had homology with KR/CK/KU_LBM255/09 strain about 99.89%. NDV surveillance is needed to investigate epidemiological relationship of domestic breeder duck isolates in Korea.

국내 종합병원부설 생명공학연구소의 GMP 시설에 대한 실태조사 및 공간구성에 대한 연구 (A Study on the Space Program & Research of GMP in Domestic Institute for Life Science)

  • 이상석;박재승
    • 의료ㆍ복지 건축 : 한국의료복지건축학회 논문집
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    • 제13권1호
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    • pp.7-15
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    • 2007
  • The medicine is used for diagnosis, treatment or prevention of disease and consequently has an important influence upon human life and health. Its effectiveness and safety must be guaranteed. In order to achieve the facility that meets GMP, KFDA has provided standards for quality assurance of the manufacture, examination and tests of medical supplies. The result of this study were as follows : the GMP facilities are divided into work, lab/test and storage spaces, and the systematic corridor. The clean rooms are mainly installed in work and lab/test spaces, and maintain class 10,000 in average. The individual space for the procedures that requires class 100 is not provided as a separate division. Instead, they are performed in the clean bench of the laboratory.

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Development of Electrical Power Subsystem of Cube Satellite STEP Cube Lab for Verification of Space-Relevant Technologies

  • Park, Tae-Yong;Chae, Bong-Geon;Oh, Hyun-Ung
    • International Journal of Aerospace System Engineering
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    • 제3권2호
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    • pp.31-37
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    • 2016
  • STEP Cube Lab (Cube Laboratory for Space Technology Experimental Project) is a 1U standardized pico-class satellite. Its main mission objective is an on-orbit verification of five fundamental core space technologies. For assuring a successful missions of the STEP Cube Lab with five payloads, electrical power subsystem (EPS) shall sufficiently provide an electrical power to payloads and bus systems of the satellite during an entire mission life. In this study, a design process of EPS system was introduced including power budget analysis considering a mission orbit and various mission modes of the satellite. In conclusion, adequate EPS hardware in compliance with design requirements were selected. The effectiveness and mission capability of EPS architecture design were confirmed through an energy balance analysis (EBA).

Dynamics and GA-Based Stable Control for a Class of Underactuated Mechanical Systems

  • Liu, Diantong;Guo, Weiping;Yi, Jianqiang
    • International Journal of Control, Automation, and Systems
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    • 제6권1호
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    • pp.35-43
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    • 2008
  • The control of underactuated mechanical system is very complex for the loss of its control inputs. The model of underactuated mechanical systems in a potential field is built with Lagrangian method and its structural properties are analyzed in detail. A genetic algorithm (GA)based stable control approach is proposed for the class of under actuated mechanical systems. The Lyapunov stability theory and system properties are utilized to guarantee the system stability to its equilibrium. The real-valued GA is used to adjust the controller parameters to improve the system performance. This approach is applied to the underactuated double-pendulum-type overhead crane and the simulation results illustrate the complex system dynamics and the validity of the proposed control algorithm.

Multi-class Analysis of Exposure Chemicals in Deciduous Teeth by Liquid Chromatography-Mass Spectrometry: Preliminary Studies on Sample Preparation Methods

  • Lee, Yujin;Seo, Eunji;Park, Jun Young;Bae, Kwang-Hak;Lee, Jaeick;Cha, Sangwon
    • Mass Spectrometry Letters
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    • 제9권4호
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    • pp.110-114
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    • 2018
  • Since accumulation of chemicals in deciduous teeth can occur from the second trimester of fetal development to shedding, a deciduous tooth has been considered as an attractive biomatrix for estimating individual chemical exposures recently. Therefore, detection of organic chemicals from teeth has received an increasing attention in exposomics research. Most previous studies on organic chemical analysis of teeth not only focused on a few targeted chemicals but also ignored potential contaminants from an enamel surface or a dental pulp. Recently, our group started developing a multi-class organic analysis method for deciduous teeth and tried to find a proper incubation condition of tooth materials. Our results showed that incubation with methanolic HCl provided the best performance among tested.

SOLUTIONS OF A CLASS OF COUPLED SYSTEMS OF FUZZY DELAY DIFFERENTIAL EQUATIONS

  • Wu, Yu-ting;Lan, Heng-you;Zhang, Fan
    • Nonlinear Functional Analysis and Applications
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    • 제26권3호
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    • pp.513-530
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    • 2021
  • The purpose of this paper is to introduce and study a class of coupled systems of fuzzy delay differential equations involving fuzzy initial values and fuzzy source functions of triangular type. We assume that these initial values and source functions are triangular fuzzy functions and define solutions of the coupled systems as a triangular fuzzy function matrix consisting of real functional matrices. The method of triangular fuzzy function, fractional steps and fuzzy terms separation are used to solve the problems. Furthermore, we prove existence and uniqueness of solution for the considered systems, and then a solution algorithm is proposed. Finally, we present an example to illustrate our main results and give some work that can be done later.

Folic acid supplementation regulates key immunity-associated genes and pathways during the periparturient period in dairy cows

  • Khan, Muhammad Zahoor;Zhang, Zhichao;Liu, Lei;Wang, Di;Mi, Siyuan;Liu, Xueqin;Liu, Gang;Guo, Gang;Li, Xizhi;Wang, Yachun;Yu, Ying
    • Asian-Australasian Journal of Animal Sciences
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    • 제33권9호
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    • pp.1507-1519
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    • 2020
  • Objective: The current research was aimed to profile the transcriptomic picture of the peripheral blood lymphocytes (PBLs) associated with immunity in Chinese Holsteins supplemented orally with coated folic acid during the periparturient period. Methods: The total of 123 perinatal cows were selected for this study and divided into three groups; group A (n = 41, 240 mg/500 kg cow/d), group B (n = 40, 120 mg/500 kg cow/d) and group C (n = 42, 0 mg/cow/d) based on the quantity of folic acid fed. Three samples of PBLs were selected from each folic acid treated group (high, low, and control) and RNA sequencing method was carried out for transcriptomic analysis. Results: The analysis revealed that a higher number of genes and pathways were regulated in response to high and low folic acid supplementation compared to the controls. We reported the novel pathways tumor necrosis factor (TNF) signaling, antigen processing and presentation, Staphylococcus aureus infection and nuclear factor (NF)-kappa B signaling pathways) and the key genes (e.g. C-X-C motif chemokine ligand 10, TNF receptor superfamily member 1A, cluster difference 4, major histocompatibility complex, class II, DQ beta, NF-kappa-B inhibitor alpha, and TNF superfamily 13) having great importance in immunity and anti-inflammation in the periparturient cows in response to coated folic acid treatment. Conclusion: Collectively, our study profiled first-time transcriptomic analysis of bovine lymphocytes and compared the involved cytokines, genes, and pathways between high vs control and low vs control. Our data suggest that the low folic acid supplementation (120 mg/500 kg) could be a good choice to boost appropriate immunity and anti-inflammation as well as might being applied to the health improvement of perinatal dairy cows.

전국치기공과의 교과과정분석과 전망 (Prospect and Analysis about curriculum of the Department of Dental Laboratory Technology in the whole country)

  • 박용덕;황경숙;김남중
    • 대한치과기공학회지
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    • 제25권1호
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    • pp.203-218
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    • 2003
  • The 3 year graduate school course of the dental technician, which has been enforced since 1994 till the present date, differs from the currently existing 2 year graduate course because of an 8 weeks clinical field training course being newly executed and an imposed curriculum about manufacturing specialized prosthetics like all-ceramic, double prosthetic technology, attachment, and implant. Even though nearly 10 years have passed, the education program of the 18 colleges have still not been standardized and the subjects differing a little, while some are still not following the 3 years graduate course. The goal of this research is to emphasize the qualities of the 3 years graduate program and enable the dental technician to handle clinical models in the dental technical clinic right after graduation by adding clinical field training in the dental technician education program of the 18 colleges. Also in accordance to a national and international increase in esthetical prosthetics, credits, weekly study hours, education practice time of specialized prosthetic dental technology can be analyzed as follows. 1. In the year 2003, currently colleges start clinical practice education starting from the 1st semester of the 2nd year junior year, to the 2nd semester of 3rd year the varying in time with adequate class time, and credits. The average credit is 9.56, and weekly average class time is 18.00 hours. later clinical trainee education will be adjusted to the 3 year graduate school course to 10 credits and 20 hours and the adequate education period should be the 2nd semester of 3rd grade when most basic clinical education has been covered. 2. Currently in the year 2003, all-ceramic education has an average credit of 8.01 in 17 colleges with an exception of Ma-san college. The weekly average theoretical education is 4.94 hours, weekly clinical education of 7.88 hours and currently in 14 colleges porcelain technical class usually starts in the 1st semester of the 2nd year. All-ceramic education is thought to have adequate credits, timing, and weekly study hours. 3. Currently in 2003 implant education is enforced in 11 colleges including Bu-san Catholic university and the education period is usually in the 3rd year. Out of the 11 colleges, only Bu-san Catholic univ., Ma-san and Shinheung college have practice training classes. We knew that Shingu, Dongu, Mokpo science colleges have practice training as a curriculum, namely specific prosthetics laboratory. Out of the 9 colleges enforcing implant education the average credits taken are 2.56, and the average hours of weekly theory education is 2.14, but with the exception of Shinheung college which has an implant practice training course theory and practice classes are not divided. Therefore implant education is thought to start in the 3rd year as a 3 credit course with 3 hours of theory and practice each. 4. Currently in 2003, theoretical attachment education is given in 15 colleges and including practical training it is given in 10 colleges. The education starts 1st semester of the 2nd year till the 2nd semester of the 3rd year. The average credit of attachment theory education in the 15 colleges was 3.64, the average weekly theoretical education was 2.64, and the average weekly practice training out of the schools teaching it was 3.20. Later attachment education is thought to start the 1st semester of the 3rd year as a 3 credit course with 2 hours of theory class and 3 hours of practice training. 5. Double prosthetic technology is currently carried out in Dae-jon Health Science college(2nd year 2nd semester, 2 credits, theory 2hours), Gimcheon college (1 credit, 2 hours of practical training), Bu-san Catholic university (4th year 2nd semester, 4 hours of theory. practice). When the 3 year course is enforced, education will start 1st semester of the 3rd year with 2hours of theory and practice each. 6. The newly installed programs of the 3 year education is clinical field training, and specialized prosthetic manufacturing practice like all-ceramic, double prosthetic technology, attachment, and implant. If such education is to be effectively managed, a primary precursor as systemic basic clinical education is thought to be important. 7. Nationally in the 18 colleges of dental technology the currently executed curriculum course had varying subjects, class time according to the subject, and credits by each college. The curriculum difference between the colleges must be settled.

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과학고등학교 화학수업에서 첨단과학 실험기기 활용 수업 전략의 개발 및 타당화: 자외선-가시광선 및 적외선 분광기를 중심으로 (The Development and Validation of Instructional Strategies Using the Advanced Laboratory Equipment(ALE) in Science High School Chemistry Classrooms: A Focus of UV-Visible and IR Spectrophotometer)

  • 전경희;박다혜;장낙한;박종욱;박종석
    • 대한화학회지
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    • 제60권1호
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    • pp.69-81
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    • 2016
  • 이 연구의 목적은 과학고등학교 화학수업에서 첨단과학 실험기기를 활용한 수업(이하 ALE 수업)전략을 개발하고 이 전략을 활용한 수업을 실시한 후, 학생들의 인식을 통해 ALE 수업 전략의 타당성을 살펴보고, ALE 수업자료 개발의 필요성을 고찰해 보는 것이다. 이를 위해 첨단과학 실험기기를 활용한 7차시의 수업을 구성하여 D과학고 21명에게 적용하고 이에 대한 설문을 실시하였다. 이후, 설문에 성실히 응답한 학생 9명을 대상으로 한 실험보고서 및 심층 면담을 통해 ALE 수업에 대한 학생들의 인식을 분석하였다. ALE 수업을 통해 학생들은 실시간으로 처리되는 데이터를 활용함으로써 수준 높은 학습개념을 쉽게 이해하게 되었고, 주변의 친숙한 물질이 실험재료가 될 수 있다는 점을 발견함으로써 실용적 탐구에 대한 안목을 갖게 되었다. 이에 따라 학생들의 과학에 대한 흥미와 자율탐구의지가 고취되었고 일부 학생은 실생활에서 발견한 일상적 문제를 검증가능한 과학적 문제로 구체화시키는 것과 같은 창의적 수행을 실시하기도 했다. 이와 같이 ALE 수업은 과학고등학교 학생들에게 과학기술에 대한 이해와 함께 과학에 대한 영역을 폭넓게 이해시키는 구체적인 방안의 하나가 될 것이다.

확인실험수업과 발견실험수업에서의 교사 발문 유형 분석 (Analysis of the Types of Teachers' Questioning in Verification Laboratory Instruction and Discovery Laboratory Instruction)

  • 김오범;안은하;김은애;고민석;양일호
    • 한국과학교육학회지
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    • 제33권7호
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    • pp.1354-1366
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    • 2013
  • 이 연구는 확인실험수업과 발견실험수업에서 교사 발문 유형을 분석하여 두 수업유형별 교사 발문이 어떠한 차이가 있는지 알아보는데 그 목적이 있다. 이 연구의 결과는 다음과 같다. 확인실험수업과 발견실험수업에서 교사들이 사용하고 있는 발문 유형의 차이는 나타나지 않았다. 또한 두 실험수업유형 모두에서 폐쇄적 발문의 사용이 많았다. 이는 교사들이 두 실험수업유형의 특징을 고려하지 않은 채, 단지 개념습득에 핵심적인 주안점을 두고 실험수업을 진행하고 있음을 알 수 있었다. 이러한 결과는 실제 확인실험수업과 발견실험수업에서 아동들의 과학적 사고를 증진시키는 기회를 적게 제공하고 있음을 나타내며 이를 보완할 수 있는 개방적 발문의 사용이 적어 아동들의 과학적 사고를 촉진시키고 있지 않음을 시사하고 있다. 따라서 교사들은 과학 실험을 위한 수업 전략 수립할 때, 실험수업유형별 특성에 적합하고 실험수업유형을 보완할 수 있는 발문 전략을 잘 계획해야 할 것이며, 이를 잘 활용한다면 과학실험수업 개선에 도움이 될 것이다.