• BMB Reports
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• v.44 no.1
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• pp.52-57
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• 2011

Termites play an important role in the degradation of dead plant materials and have acquired endogenous and symbiotic cellulose digestion capabilities. The feruloyl esterase enzyme (FAE) gene amplified from the metagenomic DNA of Coptotermes formosanus gut was cloned in the TA cloning vector and subcloned into a pET32a expression vector. The Ft3-7 gene has 84% sequence identity with Clostridium saccharolyticum and shows amino acid sequence identity with predicted xylanase/chitin deacetylase and endo-1,4-beta-xylanase. The sequence analysis reveals that probably Ft3-7 could be a new gene and that its molecular mass was 18.5 kDa. The activity of the recombinant enzyme (Ft3-7) produced in Escherichia coli (E.coli) was 21.4 U with substrate ethyl ferulate and its specific activity was 24.6 U/mg protein. The optimum pH and temperature for enzyme activity were 7.0 and $37^{\circ}C$, respectively. The substrate utilization preferences and sequence similarity of the Ft3-7 place it in the type-D sub-class of FAE.

• Korean Journal of Clinical Laboratory Science
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• v.45 no.2
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• pp.43-47
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• 2013

This study was designed to evaluate the prevalence of ESBL genes and monitor antimicrobial resistance pattern in Escherichia coli, isolated from a hospital and a community. We tested 200 E. coli strains isolated in the hospitals and community in Chungcheong area from January to March 2012. Antimicrobial susceptibilities were tested by using the disk diffusion method. A search for ESBL genes was conducted by PCR amplification, and the genotypes were determined by direct nucleotide sequence analysis of the amplified products. An Epidemiologic study was performed by repetitive extragenic palindromic sequence-based PCR (REP-PCR). The percentage of ESBL-producing isolates was 17% for hospital associated E. coli and 11% for community associated E. coli. The ESBL gene sequencing results showed that the most common ESBL in E. coli was CTX-M-14 (19/28), followed by CTX-M-15 (9/28). The REP-PCR study also showed the genetic diversity, but there was no difference between the hospital and community associated E. coli. In this study, the most common types of class A ESBLs identified were CTX-M in the hospital and the community in Chungcheong area. ESBL-producing E. coli isolates showed diverse clonality.

• Journal of Microbiology and Biotechnology
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• v.29 no.10
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• pp.1570-1579
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• 2019

The fungal products dibenzodioxocinones promise a novel class of inhibitors against cholesterol ester transfer protein (CEPT). Knowledge as to their biosynthesis is scarce. In this report, we characterized four more dibenzodioxocinones, which along with a previously described member pestalotiollide B, delimit the dominant spectrum of secondary metabolites in P. microspora. Through mRNA-seq profiling in $g{\alpha}1{\Delta}$, a process that halts the production of the dibenzodioxocinones, a gene cluster harboring 21 genes including a polyketide synthase, designated as pks8, was defined. Disruption of genes in the cluster led to loss of the compounds, concluding the anticipated role in the biosynthesis of the chemicals. The biosynthetic route to dibenzodioxocinones was temporarily speculated. This study reveals the genetic basis underlying the biosynthesis of dibenzodioxocinone in fungi, and may facilitate the practice for yield improvement in the drug development arena.

• Food Science of Animal Resources
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• v.34 no.5
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• pp.700-708
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• 2014

The volatile composition of veal has yet to be reported and is one of the important factors determining meat character and quality. To identify the most important aroma compounds in veal from Holstein bull calves fed one of three diets, samples were subjected to solid-phase microextraction (SPME) combined with gas chromatography-quadrupole mass spectrometry (GC-MS). Most of the important odorants were aldehydes and alcohols. For group A (veal calves fed entirely on milk for 90 d before slaughter), the most abundant compound class was the aldehydes (52.231%), while that was alcohols (26.260%) in group C (veal calves fed starter diet for at least 60 d before slaughter). In both classes the absolute percentages of the volatile compounds in veal were different indicating that the veal diet significantly (p<0.05) affected headspace volatile composition in veal as determined by principal component analysis (PCA). Twenty three volatile compounds showed significance by using a partial least-squared discriminate analysis (PLS-DA) (VIP>1). The establishment of the global volatile signature of veal may be a useful tool to define the beef diet that improves the organoleptic characteristics of the meat and consequently impacts both its taste and economic value.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.6
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• pp.752-756
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• 2011

Polymorphism of the second exon of the caprine leukocyte antigen-DRB3 gene (CLA-$DRB3^*02$) was investigated in this study. The 285 bp PCR product of 258 individuals from 10 domestic goat breeds in Southwest China was digested with restriction endonucleases PstI and HaeIII and then genotyped. Three alleles and 4 restriction digestion profiles were distinguished by digestion of the PCR fragment by PstI, and 8 alleles and 13 genotypes by HaeIII. For HaeIII restriction enzyme sites, the Chi-square ($X^2$) test showed that all goat breeds in this study did not fit with the Hardy-Weinberg equilibrium (p<0.01 or p<0.05). The highly polymorphic nature of CLA-$DRB3^*02$ was demonstrated and the ranges of gene heterozygosity (He) and polymorphism information content (PIC) were 0.36-0.63 and 0.32-0.55, respectively. Clustering analysis showed that the 10 goat breeds clustered into two groups and Dazu Black goat had a close genetic relationship with Chengdu Grey, Jintang Black and Nanjiang Yellow goats.

• Proceedings of the IEEK Conference
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• 2002.07a
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• pp.682-685
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• 2002

In this paper, we defined necessary operations and functional blocks of a multiplexer for 3-D video systems and present our multiplexer design. We adopted the ITU-T's recommendation(H.222.0) to define the operations and functions of the multiplexer and explained the data structures and details of the design for multiview image processing. The data structure of TS(Transport Stream) and PES (Packetized Elementary Stream) in ITU-T Recommendation H.222.0 does not fit our multiview image processing system, because this recommendation is fur wide scope of transmission of non-telephone signals. Therefore, we modified these TS and PES stream structures. The TS is modified to DSS(3D System Stream) and PES is modified to SPDU(DSS Program Data Unit). We constructed the multiplexer through these modified DSS and SPDU. The number of multiview image channels is nine, and the image class employed is MPEG-2 SD(Standard Definition) level which requires a bandwidth of 2∼6 Mbps. The required clock speed should be faster than 54(= 6 ${\times}$ 9)㎒ which is the outer interface clock speed. The inside part of the multiplexer requires a clock speed of only 1/8 of 54㎒, since the inside part of the multiplexer operates by the unit of byte. we used ALTERA Quartus II and the FPGA verification for the simulation.

• Proceedings of the KSRS Conference
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• v.2
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• pp.623-626
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• 2006

Group of genes controls the functioning of a cell by complex interactions. These interacting gene groups are called Gene Regulatory Networks (GRNs). Two previous data mining approaches, clustering and classification have been used to analyze gene expression data. While these mining tools are useful for determining membership of genes by homology, they don't identify the regulatory relationships among genes found in the same class of molecular actions. Furthermore, we need to understand the mechanism of how genes relate and how they regulate one another. In order to detect regulatory relationships among genes from time-series Microarray data, we propose a novel approach using frequent pattern mining and chain rule. In this approach, we propose a method for transforming gene expression data to make suitable for frequent pattern mining, and detect gene expression patterns applying FP-growth algorithm. And then, we construct gene regulatory network from frequent gene patterns using chain rule. Finally, we validated our proposed method by showing that our experimental results are consistent with published results.

• ETRI Journal
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• v.38 no.1
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• pp.70-80
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• 2016

This paper addresses the problem of robust waveform design for distributed multiple-radar systems (DMRSs) based on low probability of intercept (LPI), where signal-to-interference-plus-noise ratio (SINR) and mutual information (MI) are utilized as the metrics for target detection and information extraction, respectively. Recognizing that a precise characterization of a target spectrum is impossible to capture in practice, we consider that a target spectrum lies in an uncertainty class bounded by known upper and lower bounds. Based on this model, robust waveform design approaches for the DMRS are developed based on LPI-SINR and LPI-MI criteria, where the total transmitting energy is minimized for a given system performance. Numerical results show the effectiveness of the proposed approaches.

• Journal of Microbiology and Biotechnology
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• v.34 no.6
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• pp.1189-1196
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• 2024

Bacterial resistance to commonly used antibiotics is one of the major challenges to be solved today. Bacteriophage endolysins (Lysins) have become a hot research topic as a new class of antibacterial agents. They have promising applications in bacterial infection prevention and control in multiple fields, such as livestock and poultry farming, food safety, clinical medicine and pathogen detection. However, many phage endolysins display low bactericidal activities, short half-life and narrow lytic spectrums. Therefore, some methods have been used to improve the enzyme properties (bactericidal activity, lysis spectrum, stability and targeting the substrate, etc) of bacteriophage endolysins, including deletion or addition of domains, DNA mutagenesis, chimerization of domains, fusion to the membrane-penetrating peptides, fusion with domains targeting outer membrane transport systems, encapsulation, the usage of outer membrane permeabilizers. In this review, research progress on the strategies for improving their enzyme properties are systematically presented, with a view to provide references for the development of lysins with excellent performances.

• Journal of Environmental Health Sciences
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• v.30 no.5 s.81
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• pp.432-439
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• 2004

Korean certification regulation for particulate filtering respirators requires inward leakage (IL) test as European Standards (EN) and the standard levels of regulation are the same as those of EN. This study was conducted to evaluate particulate filtering respirators being commercially used in the market by using IL and assess the characteristics of IL. The study began with discussing the concept of IL, comparison of IL with fit test, and IL measurement method. Three brands of half masks and 10 brands of filtering facepieces (two top class, four 1st class and four 2nd class), total 13 brands respirators, and 10 test panels (subjects) who were classified in 9 facial grids in accordance with face length and lip length, were selected for IL test. IL tests were conducted in the laboratory of 3M Innovation Center which was established by EN standard. As expected ILs of half masks were lower than those of filtering facepiece mask. ILs of half masks and some filtering facepieces were significantly different in manufacturers. ILs of 1st class filtering facepieces were found to be much more than those of 2nd class and thus the result would cause wearers to get confused to select a mask. Four of six brands being no compliance with standards were thought that they should be tested again for certification because of a lot of differences from standards. There were no significant differences among ILs of five exercises. In 6 out of 13 brands lognormal distribution of ILs may be a better fit distribution and in 7 brands both lognormal and normal distributions were rejected. The result indicates that geometric mean may be better than arithmetic mean to establish standard.