• BMB Reports
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• 제40권6호
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• pp.845-852
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• 2007

The highly evolved flowers of orchids have colorful sepals and fused columns that offer an opportunity to discover new genes involved in floral development in monocotyledon species. In this investigation, we cloned and characterized the homologous PISTALLATA-like (PI-like) gene PhPI15 ($\underline{Ph}alaenopsis$ $\underline{PI}$ STILLATA # $\underline{15}$), from the Phalaenopsis hybrid cultivar. The protein sequence encoded by PhPI15 contains a typical PI-motif. Its sequence also formed a subclade with other monocot PI-type genes in phylogenetic analysis. Southern analysis showed that PhPI15 was present in the Phalaenopsis orchid genome as a single copy. Furthermore, it was expressed in all the whorls of the Phalaenopsis flower, while no expression was detected in vegetative organs. The flowers of transgenic tobacco plants ectopically expressing PhPI15 showed male-sterile phenotypes. Thus, as a Class-B MADS-box gene, PhPI15 specifies floral organ identity in orchids.

• BMB Reports
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• 제39권5호
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• pp.595-606
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• 2006

Metallothioneins are a group of low molecular mass and cysteine-rich metal-binding proteins, ubiquitously found in most living organisms. They play an important role in maintaining intracellular metal homeostasis, eliminating metal toxification and protecting against intracellular oxidative damages. Analysis of complete rice genome sequences revealed eleven genes encoding putative metallothionein (OsMT), indicating that OsMTs constitute a small gene family in rice. Expression profiling revealed that each member of the OsMT gene family differs not only in sequence but also in their tissue expression patterns, suggesting that these isoforms may have different functions they perform in specific tissues. On the basis of OsMT structural and phylogenetic analysis, the OsMT family was classified as two classes and class I was subdivided into four types. Additionally, in this paper we also present a complete overview of this family, describing the gene structure, genome localization, upstream regulatory element, and exon/intron organization of each member in order to provide valuable insight into this OsMT gene family.

• 대한치과기공학회지
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• 제27권1호
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• pp.115-129
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• 2005

The purpose of this study was to identify injury characteristics happened in the college dental laboratory technique classes and to provide the empirical accident findings for safety education development for the college dental technology classes. The research data were collected from a self-administered survey distributed to the conveniently selected college students. The study participants were 476 freshmen, sophomore and junior students whose major was the dental laboratory technology of the 4 colleges in 2 metropolitan cities and 2 medium-size cities. All collected survey responses were encoded and analyzed in SPSS 12.0. The findings were as follows. The accidents happened to the college students during the dental laboratory technology classes: 1. The accident rate was the highest in the sophomore and the freshmen, junior in order. 2. The body parts frequently injured were hands, face, eyes, arms, and legs in order. 3. The frequent injury types were cut, burnt, stuck, wound, and suffocated in order. 4. The treatment methods for their accidents were the simple first-aids, no treatment, and the emergency room visits in order. 5. The laboratory training conditions inducing the accidents were significantly frequent under wire-related, and wax-related, iron-related, alcohol-related jobs in order, but not frequent in investing material-related, porcelain-related, resin-related, and agar-related jobs. Polishing was the most accident-prone job explaining 63% of the laboratory accidents and then, model producing, wax patterning, casting, burning, and investing jobs in order. In summary, the college dental laboratory accidents had the certain patterns by the training level and by the material or machine involved in each class. Thus, the planned and organized safety education programs should be produced and investigated for college students before their major laboratory classes.

• Bulletin of the Korean Chemical Society
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• 제35권7호
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• pp.2057-2064
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• 2014

Thermochemical sulfate reduction (TSR) was conducted in autoclave on the system of crude oil and $MgSO_4$ at different temperatures. Gas chromatography pulsed flame photometric detector (GC-PFPD) was used to detected the composition of organic sulfur compounds in oil phase products. The results of the analysis indicate that with increased temperature, the contents of organic sulfur compounds with high molecular weight and thermal stability, such as benzothiophenes and dibenzothiophenes, gradually became dominated. In order to gain greater insight into the formation and distribution of organic sulphur compounds from TSR, positive ion electrospray Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) was used in detecting the detailed elemental composition and distribution of them. The mass spectra showed that the mass range of sulfur compounds was 200-550 Da. Four sulfur class species, $S_1$, $N_1S_1$, $O_1S_1$ and $O_2S_1$, were assigned in the positive-ion spectrum. Among the identified sulfur compounds, the $S_1$ class species was dominant. The most abundant $S_1$ class species increase associated with the DBE value and carbon number increasing which also indicates the evolution of organic sulfur compounds in TSR is from the labile series to the stable one. In pure blank pyrolysis experiments with crude oil cracking without TSR, different composition and distribution of organic sulfur compounds in oil phase products were seen from mass spectra in order to evaluate their pyrolysis behaviors without $MgSO_4$. FT-IR and XRD were used in analyzing the products of solid phases. Two distinct crystallographic phases MgO and $MgSO_4$ are found to coexist in the products which demonstrated the transformation of inorganic sulfur compounds into organosulfur compounds exist in TSR.

• Journal of Power Electronics
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• 제17권4호
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• pp.892-904
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• 2017

A design of a Class-E inverter with only one inductor and one capacitor is presented. It is operated at the optimal operation mode for domestic cooker. The design principle is based on the zero-voltage derivative switching (ZVDS) of the Class-E inverter with a parallel load network, which is a parallel resonant equivalent circuit. An induction load characterization is obtained from a large-signal excitation test bench, which is the key to an accurate design of the induction cooker system. Consequently, the proposed scheme provides a more systematic, simple, accurate, and feasible solution than the conventional quasi-resonant inverter analysis based on series load network methodology. The derivative of the switch voltage is zero at the turn-on transition, and its absolute value is relatively small at the turn-off transition. Switching losses and noise are reduced. The parameters of the ZVDS Class-E inverter for the domestic induction cooker must be selected properly, and details of the design of the components of this Class-E inverter need to be addressed. A 1,200 W prototype is designed and evaluated to verify the validation of the proposed topology.

• 교육시설
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• 제8권3호
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• pp.25-35
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• 2001

This study investigated technology education, laboratory management and facilities in middle schools with the goal of finding problems. Ninety-six randomly selected schools and questionnaire was done to the teachers of 84 schools which have laboratory. Five of these schools received on-site inspections. The results of study are as follows : 1) 12.5% of schools have no laboratory. The average laboratory has less than 50% of the necessary equipment for total operations. laboratories are used an average of once or twice per two time frame. 2) 80%of the laboratories are located at the end of a row of regular class rooms. The most common laboratory style is classroom plus hallway($90m^2$) style. 77% of laboratories have a single entrance. 3) 69% of teachers reported the worktable arrangement to be inconvenience. 68% of the schools have 9 worktables per laboratory. 38% say the worktables are not wide enough and 36% said they were appropriate. Table dimensions range from $95{\sim}110cm$ in width and from $210{\sim}225cm$ in length. 65% of the teachers say table height is low with a range from $76{\sim}82cm$. 86% of the teachers say that the height of chair is low and the range of its measurement is from $37{\sim}47cm$. 84% of the teachers points out that laboratory is poorly lighted.

• 대한의생명과학회지
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• 제26권4호
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• pp.288-295
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• 2020

Infection of Helicobacter pylori on gastric mucosa is associated with various gastric diseases. According to the WHO, H. pylori causes gastric cancer and has been classified as a class I carcinogen. Riboflavin is an essential vitamin which presents in a wide variety of foods. Previous studies have shown that riboflavin/UVA was effective against the growth inhibition of Staphylococcus aureus, S. epidermidis and multidrug-resistant Pseudomonas aeruginosa and had the potential for antimicrobial properties. Thus, we hypothesized that riboflavin has a potential role in the growth inhibition of H. pylori. To demonstrate inhibitory concentration of riboflavin against H. pylori, we performed agar and broth dilution methods. As a result, we found that riboflavin inhibited the growth of H. pylori. The MIC was 1 mM in agar and broth dilution test. Furthermore, to explain the inhibitory mechanism, we investigated whether riboflavin has an influence on the replication-associated molecules of the bacteria using RT-PCR to detect mRNA expression level in H. pylori. Riboflavin treatment of H. pylori led to down-regulation of polA and dnaB mRNA expression levels in a dose dependent manner. After then, we also confirmed whether riboflavin has cytotoxicity to human cells. We used AGS, a gastric cancer cell line, and treated with riboflavin did not show statistically significant decrease of cell viability. Thus, these results indicate that riboflavin can suppress the replication machinery of H. pylori. Taken together, these findings demonstrate that riboflavin inhibits growth of H. pylori by inhibiting replication of the bacteria.

• 한국항공우주학회지
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• 제42권7호
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• pp.622-627
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• 2014

극초소형 위성으로 분류되는 큐브위성의 경우, 표준화된 위성의 크기로 인하여 위성의 전력생성을 목적으로 하는 태양전지판 장착을 위한 공간이 극히 제한적이며, 자세제어 적용 방식에 따라서는 태양전지판에 입사되는 태양광의 각도가 변화하고 이는 태양전지의 전력생성 양을 결정하는 주요 요인으로 작용한다. 본 논문에서는 극초소형 위성 적용을 목적으로 태양광과 태양전지판이 이루는 각도가 $0^{\circ}$인 조건에서도 태양전지판 외곽에 배치된 렌즈어레이를 통해 태양광을 효율적으로 조사하여 전력생성 효율 향상이 가능한 우주용 집광형 태양전력 시스템을 제안하였으며, 극초소형 위성으로의 적용 가능성 검토를 위해 상용 렌즈어레이를 적용한 기능시험을 통해 유효성을 입증하였다.

• BMB Reports
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• 제39권6호
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• pp.749-758
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• 2006

The cytosolic members of the HSP70 family of proteins play key roles in the molecular chaperone machinery of the cell. In the study we cloned and sequenced the full-length cDNA of Delia antiqua HSP70 gene, which is 2461 bp long and encodes 643 a.a. with a calculated molecular mass of 70,787 Da. We investigated gene copies of cytosolic HSP70 members of 4 insect species with complete genome available, and found that they are quite variable with species. In order to characterize this protein we carried out an alignment and a phylogenetic analysis with 41 complete protein sequences from insects. The analysis divided the cytosolic members of the family into two classes, HSP70 and HSC70, distinguishable on the basis of 15 residues. HSP70 class members were slightly shorter in length and smaller in molecular mass relative to the HSC70 class members, and the conservative and functional regions in these sequences were documented. Mainly, we investigated the expression of Delia antiqua HSP70 gene, in response to diapauses and thermal stresses. Both summer and winter diapauses elevated HSP70 transcript levels. Cold-stress led to increased HSP70 expression levels in summer- and winter-diapausing pupae, but heat-stress elevated the levels only in the winter-diapausing pupae. In all cases, the expression levels, after being elevated, gradually decreased with time. HSP70 expression was low in non-diapausing pupae but was up-regulated following cold- and heat-stresses. Heat-stress gradually increased the mRNA level with time whereas cold-stress gradually decreased levels after an initial increase.

• 신재생에너지
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• 제17권4호
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• pp.1-8
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• 2021

To determine the wind turbine class in the offshore of the Korean Peninsula, the reference wind speed for a 50-y return period at the hub height of a wind turbine was estimated using the reanalysis data sets. The most recent reanalysis data, ERA5, showed the highest correlation coefficient (R) of 0.82 with the wind speed measured by the Southwest offshore meteorological tower. However, most of the reanaysis data sets except CFSR underestimated the annual maximum wind speed. The gust factor of converting the 1 h-average into the 10 min-average wind speed was 1.03, which is the same as the WMO reference, using several meteorological towers and lidar measurements. Because the period, frequency, and path of typhoons invading the Korean Peninsula has been changing owing to the climate effect, significant differences occurred in the estimation of the extreme wind speed. Depending on the past data period and length, the extreme wind speed differed by more than 30% and the extreme wind speed decreased as the data period became longer. Finally, a reference wind speed map around the Korean Peninsula was drawn using the data of the last 10 years at the general hub-height of 100 m above the sea level.