• 제목/요약/키워드: Laboratory Test

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인삼 육성계통의 Fusarium sozani에 의한 근부병 저항성 비교 (Comparison of Resistance of Root Rot Caused by Fusarium solani in Ginseng Breeding Lines)

  • 천성룡;김홍진
    • Journal of Ginseng Research
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    • 제14권1호
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    • pp.50-56
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    • 1990
  • Root-rot of ginseng caused by Fusarium solani is one of the most obstacles to ginseng cultivation. We evaluated some inoculating techniques of ginseng with Fusarium solani, for selection of disease resistant breeding lines. The most effective inoculating techniques evaluated were inserting toothpicks colonized by F. solani into the seedling roots in laboratory test and dusting seedlings with vermiculite after dipping in conidial sllspension and then replanting method in field test. The resistance to diseased by F. solani was lines of 82022 and 82066 in laboratory test. 82920-1 and 78093 in field test.

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제동 라이닝애 대한 시험소간 비교에 의한 숙련도시험 (Proficiency Testing by Inter-Laboratory Comparisons for Braking Lining)

  • 정종덕;권성태;홍용기;김원경
    • 한국철도학회:학술대회논문집
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    • 한국철도학회 2003년도 춘계학술대회 논문집
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    • pp.586-591
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    • 2003
  • This paper describes the result of proficiency testing by inter-laboratory comparisons for braking lining. Proficiency testing provides an objective means of assessing and demonstrating the validity and reliability of test results produced by laboratories. To more accuracy comparison test for braking lining test, they will be made the same test procedure and environment conditions.

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Shear bond strength of zirconia to resin: The effects of specimen preparation and loading procedure

  • Chen, Bingzhuo;Yang, Lu;Lu, Zhicen;Meng, Hongliang;Wu, Xinyi;Chen, Chen;Xie, Haifeng
    • The Journal of Advanced Prosthodontics
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    • 제11권6호
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    • pp.313-323
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    • 2019
  • PURPOSE. Shear bond strength (SBS) test is the most commonly used method for evaluating resin bond strength of zirconia, but SBS results vary among different studies even when evaluating the same bonding strategy. The purpose of this study was to promote standardization of the SBS test in evaluating zirconia ceramic bonding and to investigate factors that may affect the SBS value of a zirconia/resin cement/composite resin bonding specimen. MATERIALS AND METHODS. The zirconia/resin cement/composite resin bonding specimens were used to simulate loading with a shear force by the three-dimensional finite element (3D FE) modeling, in which stress distribution under uniform/non-uniform load, and different resin cement thickness and different elastic modulus of resin composite were analyzed. In vitro SBS test was also performed to validate the results of 3D FE analysis. RESULTS. The loading flat width was an important affecting factor. 3D FE analysis also showed that differences in resin cement layer thickness and resin composite would lead to the variations of stress accumulation area. The SBS test result showed that the load for preparing a SBS specimen is negatively correlated with the resin cement thickness and positively correlated with SBS values. CONCLUSION. When preparing a SBS specimen for evaluating bond performance, the load flat width, the load applied during cementation, and the different composite resins used affect the SBS results and therefore should be standardized.

ISO/IEC 25023 소프트웨어 품질 특성 평가를 이용한 시험자간 비교시험에 관한 연구 (A Study on Proficiency Comparison Testing Between Testers Using ISO/IEC 25023 Software Quality Characteristics Evaluation)

  • 이창희;김명섭;김윤숙;노아름;양정심;송성현
    • 한국산학기술학회논문지
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    • 제21권6호
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    • pp.18-27
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    • 2020
  • 본 연구는 국제표준 ISO/IEC 25023 소프트웨어 품질 특성 평가를 이용하여 ISO/IEC 17043 숙련도 시험 요구 사항을 기준으로 시험자간 숙련도 시험의 결과를 비교 분석한 연구이다. 시험 분야 신청서는 KOLAS에서 정의하는 전기 시험 분야의 소프트웨어 시험으로 한국계량측정협회에 제출을 하였다. 시험자간 숙련도 시험 평가 항목은 국제표준인 ISO/IEC 25023 SW 품질 특성 중 기능 완전성과 시간 효율성으로 선정을 하였다. 시험자간 숙련도 비교 시험은 서울 소재의 LG전자 SW공인시험소에서 2019년 5월 28일에 KOLAS 평가사가 현장에 입회하여 실시되었다. 시험 평가 방법은 기능 완전성으로 시험자 1인당 1회 측정을 하였고 시간 효율성으로 시험자 1인당 10회를 반복 측정을 하였다. 시험의 수행 결과 기능 완전성의 경우는 12개의 시험 단계를 시험자들이 모두 통과하여 만족으로 평가되었다. 시간 효율성은 10회 반복 측정한 평균 결과 시험자 1과 3은 9.45 ± 0.01 초, 시험자 2는 9.52 ± 0.0 초로 나타났으나 통계적으로 유의하게 차이가 없었다. 결론적으로 시험자들의 숙련도는 동등하였으며 해당 시험기관 시험 결과의 신뢰성을 확인할 수 있었다.

ADVANCED TEST REACTOR TESTING EXPERIENCE - PAST, PRESENT AND FUTURE

  • Marshall Frances M.
    • Nuclear Engineering and Technology
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    • 제38권5호
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    • pp.411-416
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    • 2006
  • The Advanced Test Reactor (ATR), at the Idaho National Laboratory (INL), is one of the world's premier test reactors for providing the capability for studying the effects of intense neutron and gamma radiation on reactor materials and fuels. The physical configuration of the ATR, a 4-leaf clover shape, allows the reactor to be operated at different power levels in the comer 'lobes' to allow for different testing conditions for multiple simultaneous experiments. The combination of high flux (maximum thermal neutron fluxes of 1E15 neutrons per square centimeter per second and maximum fast [E>1.0 MeV] neutron fluxes of 5E14 neutrons per square centimeter per second) and large test volumes (up to 122 cm long and 12.7 cm diameter) provide unique testing opportunities. The current experiments in the ATR are for a variety of test sponsors - US government, foreign governments, private researchers, and commercial companies needing neutron irradiation services. There are three basic types of test configurations in the ATR. The simplest configuration is the sealed static capsule, which places the capsule in direct contact with the primary coolant. The next level of experiment complexity is an instrumented lead experiment, which allows for active control of experiment conditions during the irradiation. The most complex experiment is the pressurized water loop, in which the test sample can be subjected to the exact environment of a pressurized water reactor. For future research, some ATR modifications and enhancements are currently planned. This paper provides more details on some of the ATR capabilities, key design features, experiments, and future plans.

부산점토의 Fall Cone Test에 의한 지수 및 강도특성 (Index Properties and Undrained Shear Strength of the Pusan Clays by Fall Cone Test)

  • 정승원;정성교;;곽정민;김무겸
    • 한국지반공학회:학술대회논문집
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    • 한국지반공학회 2000년도 가을 학술발표회 논문집
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    • pp.391-398
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    • 2000
  • The index properties of cohesive soils play an important role to examine entire tendency of soil properties. Especially, the Atterberg limits have a good correlation with compression and shear strength of cohesive soils. However, these values strongly depend on their testing methods. In this study, for Pusan clays the Atterberg limits were peformed under different conditions; for example, four kinds of specimen preparation, two kinds of testing equipment, and four kinds of estimating method. And a laboratory vane test was peformed to compare the undrained shear strength with that of the fall cone test. As experimental results, the value of liquid limit performed for oven-dried sample, followed in ASTM D4318, underestimated by about 10% compared to those of another three methods, irrespective to the used equipments. But the value of plastic limit was not influenced by sample preparation methods and equipments. The liquid limits by one-point methods(Leroueil et al., 1996; Nagaraj et al., 1981) were agreed well with those of different methods. Finally, the undrained shear strength by laboratory vane test was relatively larger than that of fall cone test, and the relationship between both showed a bad trend.

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산전 태아 진단을 위한 양수의 세포유전학적 분석 (Cytogenetic and Clinical Analysis for Antenatal Diagnosis in Amniotic Fluid)

  • 오현숙;김미경;김성미
    • 대한임상검사과학회지
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    • 제39권3호
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    • pp.151-155
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    • 2007
  • Diagnosis and prevention of cytogenetics diseases are one of the most important parts in prenatal care. For that reason, it is necessary to examine birth defects. However, there is no reliable statistical data about birth defects in our country. In this study, the ratio of birth defects were determined by cytogenetics analysis and amniocentesis, in addition, the usefulness of amniocentesis was analyzed. The screening test and the triple marker test were conducted for 3,325 pregnant women of between 15 and 22 weeks gestation. Amniocentesis was performed for 170 pregnant women who were positive in the two tests, 184 women of advanced maternal age and 48 women with family history of chromosome aberrations. Among 419 women, 8 pregnant women who were positive in the triple marker test, 1 woman who close to the cut-off value in the triple marker test, 2 women with advanced maternal age and 1 woman who has history of chromosome aberration pregnance that was positive in cytogenetics analysis. The overall incidence of chromosomal aberration was 12 cases including 7 cases of Down's syndrome, 1 case of Patau syndrome, 1 case of Klinefelter syndrome, 1 case of Edward syndrome, 1 case of Robertsonian translocation and 1 case of XYY syndrome. These results show that amniocentesis for pregnant women who need chromosome test in prenatal cytogenetics analysis is very useful.

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치아 근관 감염 검체에서 검은 색소 형성 Prevotella species와 Porphyromonas species의 동정 (Identification of the Black-pigmented Prevotella Species and Porphyromonas Species from Infected Dental Root Canals)

  • 김은숙;김신무
    • 대한임상검사과학회지
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    • 제38권1호
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    • pp.45-53
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    • 2006
  • Anaerobic black-pigmented bacteria have been implicated in the endodontic infections. This group of microorganisms includes Porphyromonas endodontalis, P. gingivalis, Prevotella intermedia, and Prevotella nigrescens. The organisms display a wide variety of virulence factors that may be pertinent to acute endodontic infections. The aim of this study was to identify P. endodontalis, P. gingivalis, P. intermedia, and P. nigrescens by using the special potency disk test, filter paper spot test, 16S rRNA gene-directed PCR, and API 32A system. Microbial samples were collected from root canals of 33 intact teeth with necrotic pulp and apical periodontitis. Conventional laboratory methods were used to identify the strains of anaerobic black pigmented bacteria. Eighteen out of 33 samples were positive for the growth of black-pigmented bacrteria. Five colonies were cultured from each pure cultured colony from Brucella agar plates. Seventy seven colonies were positive for the growth of black-pigmented bacteria. Thirty three out of 77(42.8%) were identifed as P. nigrescens, 10 out of 77(13%)were P. gingivalis, 6 out of 77(7.8%) were P. endodontalis, 10 out of 77(13%) were P. intermedia. On the contrary the reference strains of P. nigrescens, experimental strains of P. nigrescens were susceptible to kanamycin in the special potency disk test. We concluded that after rapid presumptive identification methods, such as the special potency disk test and filter paper spot test were done, 16S rRNA gene PCR and API 32A test would be accurate detection methods for black-pigemented bacteria.

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Agreement of three commercial anti-extractable nuclear antigen tests: EUROASSAY Anti-ENA Profile, Polycheck Autoimmune Test and FIDIS Connective Profile

  • Kim, Namhee;Kim, In-Suk;Chang, Chulhun L;Kim, Hyung-Hoi;Lee, Eun Yup
    • 고신대학교 의과대학 학술지
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    • 제33권3호
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    • pp.307-317
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    • 2018
  • Background: Detection of antibodies to extractable nuclear antigens (ENAs) is needed for the diagnosis in systemic autoimmune diseases. In this study, we compared three reagents using line immunoblot assay (LIA) or multiplex bead immunoassay for detecting the anti-ENAs. Methods: A total of 89 sera were tested by 3 different assays: EUROASSAY Anti-ENA Profile (Euroimmune, Germany), Polycheck Autoimmune Test (Biocheck GmbH, Germany), and $FIDIS^{TM}$ Connective Profile (Biomedical Diagnostics, France). The following individual ENAs were investigated: Sm, SS-A (Ro), SS-B (La), Scl-70, Jo-1 and RNP. We reviewed medical records to investigate the discrepant results among three methods. Results: Overall percent agreements were 96.1% between EUROASSAY Anti-ENA Profile and $FIDIS^{TM}$ Connective profile; 90.4% between EUROASSAY Anti-ENA Profile and Polycheck Autoimmune Test using the manufacturers' cutoff; 96.4% between EUROASSAY Anti-ENA Profile and Polycheck Autoimmune Test using a upward cutoff; 90.4% between $FIDIS^{TM}$ Connective profile and Polycheck Autoimmune Test the manufacturers' cutoff; and 96.4% between $FIDIS^{TM}$ Connective profile and Polycheck Autoimmune Test a upward cutoff. Conclusions: The three assays showed excellent agreement with each other. With appropriate cutoff, the all three assays for six of the anti-ENA tests investigated in this study can be used in clinical laboratories for detecting the anti-ENAs.

Effects of Olaquindox and Cyadox on Immunity of Piglets Orally Inoculated with Escherichia coli

  • Ding, Mingxing;Yuan, Zonghui;Wang, Yulian;Zhu, Huiling;Fan, Shengxian
    • Asian-Australasian Journal of Animal Sciences
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    • 제18권9호
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    • pp.1320-1325
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    • 2005
  • A 2${\times}$3 factorial arrangement of treatments was used to determine the effects of olaquindox and cyadox on immune response of Landrace${\times}$Large-White geld piglets that had been orally given 10$^{10}$ CFU of Escherichia coli (E. coli, O$_{139}$:K$_{88}$). Factors included (1) E. coli inoculation or control, and (2) no antimicrobials, 100 mg/kg olaquindox and 100 mg/kg cyadox in the basal diet respectively. E. coli inoculums were orally administered 7 days after the diets were supplemented with olaquindox and cyadox. The effects of the two antimicrobials were assessed in terms of: (1) average daily gain (ADG), (2) systemic immune response (the number of white blood cells and lymphocytes, leukocyte bactericidal capacity, lymphocyte proliferation response to PHA, immunoglobulin concentrations, and total serous hemolytic complement activity), and (3) intestinal mucosal immunity including the number of intraepithelial lymphocytes (IELs) and immunoglobulin A secreting cells (ASCs) in the intestinal lamina propria. E. coli inoculation reduced ADG (p<0.05) during the period of d 0 to d 14 after the challenge while the antimicrobial supplementations improved ADG (p<0.01) during the experiment. ADG in cyadox-supplemented pigs was higher (p<0.05) than that in olaquindox-supplemented pigs. The antimicrobials decreased IEL and ASC counts in the jejunum and ileum (p<0.01) while E. coli inoculation caused them to increase (p<0.01). Jejunal ASCs in the cyadox-supplemented pigs were lower (p<0.05) than those in the olaquindox-supplemented. E. coli elicited increase (p<0.05) in white blood cell counts, leukocyte bactericidal capacity, lymphocyte proliferation rate, serous IgA concentrations, and serous hemolytic complement activity. The antimicrobials decreased the measured systemic immune parameters, but not significantly (p>0.05). The data suggest that olaquindox and cyadox suppress E. coli-induced immune activation, especially intestinal mucosal immune activation, which may be involved in the observed growth promotion.