• 대한임상검사과학회지
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• 제41권2호
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• pp.62-66
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• 2009

HbA1c is the major fraction of glycated hemoglobin, and used primarily to identify the average plasma glucose concentration over prolonged periods of time, mostly 2-3 months. It is used as markers for the diagnosis and monitoring of diabetic patients and increasingly used a marker in health screening check up for general populations. In this study, HbA1c was measured with Cobas integra 800 (Roche Diagnostics, Mannheim, Germany), using immunoturbidimetry principles. We established the reference interval for HbA1c with Cobas integra 800 and evaluated its significance. The study subjects were 36,140 (male 57.5%, and female 42.2%) who visited the Health Promotion Center of a tertiary care center in Seoul for health checkup from January to September, 2008. HbA1c levels were measured with immunoturbidimetric method. Statistical evaluation was done with SPSS. Comparison between male and female was checked with Mann-Whitney test, and among age groups with Kruskal-Wallis test. Reference interval for HbA1c was from 4.8% to 6.1%. There was no significant difference between male and female with Mann-Whitney test (P=0.539). There was significant difference among age groups with Kruskal-Wallis test (P<0.05). Reference interval for HbA1c with Cobas integra 800 was 4.8~6.1%, which was different from conventional one, 4.4-6.4%. Establishment of reference interval for each principle is needed.

• 대한임상검사과학회지
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• 제48권1호
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• pp.36-40
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• 2016

적혈구 동종면역은 수혈자와 공여자간의 적혈구 항원의 차이이다. 수혈을 위해서는 혈구형과 혈청형이 일치하지 않으면 적혈구 항체 선별 검사가 필요하며 불일치의 원인을 해명하는 것이 필수적이다. 적혈구 항체 선별검사는 임상적으로 유의한 항체를 검출하고, 신속 정확하며, 신뢰할 수 있는 방법으로 수혈에 앞서 우선적으로 하는 것을 권장한다. 본 연구자들은 다빈도로 검출된 E, D, M, E+c, C+e 항체에 대한 선별검사를 보고하였다. 따라서 이러한 결과로 항-D, 항-E의 항체가 신생아 용혈성 질환, 지연형 용혈성 수혈부작용 반응의 위험요인으로 인식하고자 한다. 또한 혈액안전관리를 적용하기 위해 적합한 항체선별검사가 요구되고, 수혈 위험요인의 선별에 있어 더 나은 효율성을 제공할 것이며 향후에 각 국가에 따라 실질적인 검출빈도를 찾는 연구가 필요할 것으로 사료된다.

• Parasites, Hosts and Diseases
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• 제51권4호
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• pp.485-488
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• 2013

The performance values of available techniques used in serodiagnosis of toxoplasmosis are satisfactory but they raise problems of equivocal and discordant results for very low IgG titers. Recently marketed, LDBio-Toxo II IgG Western blot (IB) showed an excellent correlation with the dye test. We estimated the proportion of equivocal and discordant results between the enzyme immunoassay Platelia Toxo IgG (EIA-IgG) and fluorescent antibody test (FAT) and assessed the usefulness of the IB as a confirmatory test. Out of 2,136 sera collected from pregnant women, 1,644 (77.0%) tested unequivocally positive and 407 (19.0%) were negative in both EIA-IgG and FAT. The remaining 85 (4%) sera showed equivocal or discordant results. Among them, 73 (85.9%) were positive and 12 (14.1%) were negative in IB. Forty-one (89.1%) equivocal sera in EIA-IgG and 46 (86.8%) equivocal sera in FAT were positive in IB. Reducing the cut-off values of both screening techniques improved significantly their sensitivity in detecting very low IgG titers at the expense of their specificity. In conclusion, equivocal results in routine-used techniques and their discordance in determination of the immune status in pregnancy women were not uncommon. IB test appeard to be highly useful in these situations as a confirmatory technique.

• 대한전자공학회논문지TC
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• 제48권12호
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• pp.1-8
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• 2011

일반 산업분야에서는 활발히 진행되고 있는 KOLAS(Korea Laboratory Accreditation Scheme) 시험기관 인정이 스위치, 라우터등의 네트워크장비 분야에서는 인정 현황을 찾아 볼수 없다. 즉 기관의 자체 시험이나 BMT(Bench Marking Test)를 통하여 네트워크장비에 대한 평가를 진행하고 있지만 공인 시험기관에서 발행하는 시험성적서와는 품질에 차이가 있다. 공공기관 또는 기타 사업자가 요청하는 공인기관 시험성적서의 수요가 증가함에 따라 국내에서도 네트워크 장비에 대한 공인시험기관 인정이 조속히 이루어 져야 한다. 따라서 본 논문에서는 한국인정기구에서 요구하는 네트워크장비 분야에 대한 국제공인 시험기관 인정 추진 과정에서 분석한 측정불확도와 프로그램을 통해, 효율적인 추진 방향을 제시하고자 한다.

• 대한임상검사과학회지
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• 제36권2호
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• pp.137-143
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• 2004

Erythrocyte sedimentation rate(ESR) measurements were performed in a total of 191 blood samples from patients below a hematocrit of 35% by both the Test-1(SIRE Analytical Systems, Udine, Italy) and the Westergren method, endorsed as the reference method for ESR by the International Council for Standardization in Hematology(ICSH). The corrected Westergren ESR values were also obtained applying the formula of Fabry (corrected ESR = ESR measured x 15/55-Hct). Linear regression analysis showed a close correlation (r=0.85) between the two methods both before and after ESR correction in total samples. There was no significant correlation difference between two methods in both two groups with hemoglobin $${\geq_-}9g/dl$$ and <9g/dl before and after ESR correction (r=0.873, r=0.827 respectively before correction and r=0.867, r=0.830 respectively after correction). Also, for two groups with hematocrit $${\geq_-}24%$$ and <24%, no significant difference was observed (r=0.859, r=0.792 respectively before correction and r=0.782, r=0.842 respectively after correction). However, for samples with <60mm of Westergren ESR before correction, a better correlation coefficient was obtained than samples with $${\geq_-}60mm$$(r=0.701, r=0.541 respectively). In corrected Westergren ESR, also samples that were obtained with <40mm showed better correlation than samples with $${\geq_-}40mm$$ (r=0.690, r=0.347 respectively). In conclusion, we found a good correlation between Test-1 and Westergren measurements in patients with anemia but an expert group discussion is required to solve discrepancy between two methods in blood samples with very high ESR.

• Corrosion Science and Technology
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• 제8권6호
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• pp.243-250
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• 2009

When a steel sheet pipe applied to marine environment, an anti-corrosive coating should be treated to obtain long-term life-time for steels, especially, splash zone. Although anti-corrosive property of coatings is required to be tested in real marine environment, it is difficult because of long test time such as 20 years or more time. Therefore, we used cyclic corrosion tester in a laboratory, which has similar conditions with salt-dry-wet process such as real marine environment. Anti-corrosive properties of the coatings and two steels were tested their anti-corrosive properties under cyclic corrosion test conditions(KS D ISO 14993) and the results were compared with estimate life-time in real marine environment. According to cyclic corrosion test, accelerated corrosive factor of each anti-corrosive coating was investigated accelerated corrosive factor from impedance with EIS method. Accelerated corrosive factor of type SS400 carbon steel and A690 was also investigated their accelerated corrosive factor from the regression curves of weigh loss results. One of the anti-corrosive coatings showed about 50 years life-time compared with standard sample life-time. Carbon steel SS400 showed from 0.1 mm/yr to 0.06 mm/yr as its corrosion rate.

• 한국식품과학회지
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• 제37권4호
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• pp.532-536
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• 2005

우리나라에서 사용하는 수입원맥에 잔류하는 알루미늄포스 파이드에 대해 모니터링을 실시하였으며 원맥과 함께 밀가루에 대하여도 포스파인의 잔류량을 모니터링하였다. 묽은 황산과 시료 중의 잔류 포스파인을 반응시켜 발생된 포스파인가스를 GC-NPD를 이용하여 측정하였다. 호주산 밀의 경우 전체 27건의 시료 중 95% 이상이 1 ppb 이하로 검출되었다. 그러나 미국산 밀의 경우 58건중 70% 정도가 1-10ppb 범위에서 검출되었으며, 10ppb가 넘는 시료도 4건이나 되는 등 상대적은 높은 수준의 알루미늄포스파이드 잔류량을 나타내었다. 원료에서 제품으로 포스파인이 이행되는 정도는 14-22%인 것으로 나타났으며 모의실험을 통하여 제분 중에 제거되는 포스파인의 양에는 한계가 있음을 알 수 있었다. 같은 산지의 밀이라도 다양한 농도 범위에서 불규칙한 잔류량을 보이고 있었으며 동일 물량이라 하더라도 포스파인의 잔류량은 균질하지 않음을 확인하여 알루미늄포스파이드제제를 사용하여 훈증소독을 실시할 경우, 훈증방법이나 훈증시간 이외에도 훈증 후의 개방시간, 주위환경 등이 매우 중요함을 알 수 있었다.

• 대한임상검사과학회지
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• 제37권3호
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• pp.220-227
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• 2005

Recently, the use of an electrical apparatus has brought up concerns of health risks from exposure to electromagnetic fields. EMF is composed of electric fields and magnetic fields. Heavy exposure to EMF can occur only in the vicinity of high-voltage overhead transmission lines, close to transformers and underground cables, and also close to large electrical machinery. In this thesis I have investigated the hypothesis of the correlation between occupational exposure to ELF-EMF and the risks of leukemia, anemia, cancer. Therefore, the aim of this study is to investigate whether or not ELF-EMF emitted from electric power stations and transformer substations affect some hematological parameters and tumor markers of electric utility workers. The hematological test results and tumor markers under investigation were similar in the two groups but some of parameters such as RBC, AFP, LDH showed significant difference between the two groups from two sample t-test (p<0.05). The exposure group showed increased LDH level compared to the control group by two sample t-tests. In addition, the abnormal LDH level in the exposure group was observed to be clinically significant by ${\chi}^2$-test. However, the levels of RBC, AFP observed were not clinically significant by ${\chi}^2$-test (p>0.05). These results suggested that ELF-EMF does not affect most blood test parameters except LDH of electric utility workers.

• Smart Structures and Systems
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• 제23권2호
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• pp.107-122
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• 2019

As one of the most important parameters in structural health monitoring, structural frequency has many advantages, such as convenient to be measured, high precision, and insensitive to noise. In addition, frequency-change-ratio based method had been validated to have the ability to identify the damage occurrence and location. However, building a precise enough finite elemental model (FEM) for the test structure is still a huge challenge for this frequency-change-ratio based damage detection technique. In order to overcome this disadvantage and extend the application for frequencies in structural health monitoring area, a novel method was developed in this paper by combining the cross-model cross-mode (CMCM) model updating algorithm with the frequency-change-ratio based method. At first, assuming the physical parameters, including the element mass and stiffness, of the test structure had been known with a certain value, then an initial to-be-updated model with these assumed parameters was constructed according to the typical mass and stiffness distribution characteristic of shear buildings. After that, this to-be-updated model was updated using CMCM algorithm by combining with the measured frequencies of the actual structure when no damage was introduced. Thus, this updated model was regarded as a representation of the FEM model of actual structure, because their modal information were almost the same. Finally, based on this updated model, the frequency-change-ratio based method can be further proceed to realize the damage detection and localization. In order to verify the effectiveness of the developed method, a four-level shear building was numerically simulated and two actual shear structures, including a three-level shear model and an eight-story frame, were experimentally test in laboratory, and all the test results demonstrate that the developed method can identify the structural damage occurrence and location effectively, even only very limited modal frequencies of the test structure were provided.

• Toxicological Research
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• 제35권3호
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• pp.215-224
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• 2019

As various populations are rapidly becoming an aging society worldwide and interest in health issues has increased, demand for functional foods including herbal products has increased markedly to maintain a healthy state which has led to safety issues about their intake as an inevitable result. The objective of this study was to identify the safety profile of a Korean red ginseng and Salvia plebeia R. Br. extract mixture (KGC-03-PS) which is a valuable ingredient that can be used as a functional food. In the present study, the subacute oral toxicity and bacterial reverse mutagenicity of KGC-03-PS were evaluated. Sprague Dawley rats were administered KGC-03-PS orally for 28 days by gavage. Daily KGC-03-PS dose concentrations were 0, 500, 1,000, or 2,000 mg/kg body weight (bw) per day. Bacterial reverse mutation test with KGC-03-PS dose levels ranging from 312.5 to $5,000{\mu}g/plate$ was carried out by OECD test guideline No. 471. Five bacterial strains (Salmonella typhimurium TA98, TA100, TA1535, TA1537, and Escherichia coli WP2) were tested in the presence or absence of metabolic activation by plate incorporation method. There were no toxicological effects related with test substance in the clinical evaluation of subacute oral toxicity test including clinical signs, body weight, and food consumption. Moreover, no toxicological changes related to KGC-03-PS were observed in the hematological and serum biochemical characteristics as well as in the pathological examinations, which included organ weight measurements and in the gross- or histopathological findings. KGC-03-PS did not induce an increase in the number of revertant colonies in all bacterial strains of the bacterial reverse mutation test. The no-observed-adverse-effect level of KGC-03-PS is greater than 2,000 mg/kg bw/day, and KGC-03-PS did not induce genotoxicity related to bacterial reverse mutations under the conditions used in this study.