• Korean Journal of Clinical Laboratory Science
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• v.53 no.1
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• pp.68-80
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• 2021

An uncertified facility is a facility not certified, as defined in the Biological Agents and Toxins Act (BATA) in Singapore, but has met the requirements of the Ministry of Health to possess First and Fifth Schedule biological agents and toxins. This type of facility is also known as a Biosafety Level 2 Plus (BSL 2+) facility. Registration as an uncertified facility or a BSL 2+ facility requires a certain process and procedure to be sought with the Biosafety Branch of the ministry. This review, shares first-hand knowledge on the journey to achieving registration of the authors' facility. The procedure involved considerable preparation, setting up facility requirements, biosafety precautions, procedures and practices, and training and competence of laboratory users. The ministry conducted a thorough onsite facility audit to ensure that the facility requirements and biosafety procedures and practices were in place. It then issued an approval letter of possession for the first-time use of biological agents and registered the laboratory as an uncertified facility. The expectation is that the comprehensive information shared may be of great benefit to other facilities with similar interests.

• BMB Reports
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• v.41 no.10
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• pp.733-738
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• 2008

Although previous studies have implicated a role for TC1 (C8orf4) in cancer cell proliferation, the molecular mechanism of its action is still largely unclear. In this study, we showed, for the first time, that the mRNA levels of TC1 were upregulated by mitogens (FBS/thrombin) and at least partially, through the ERK1/2 signaling pathway. Interestingly, the over-expression of TC1 promoted the $G_1$- to S-phase transition of the cell cycle, which was delayed by the deficiency of ERK1/2 signaling in fibroblast cells. Furthermore, the luciferase reporter assay indicated that the over-expression of TC1 significantly increased Cyclin D1 promoter-driven luciferase activity. Taken together, our findings revealed that TC1 was involved in the mitogen-activated ERK1/2 signaling pathway and positively regulated $G_1$- to S-phase transition of the cell cycle. Our results may provide a novel mechanism of the role of TC1 in the regulation of cell proliferation.

• Journal of the Korean Institute of Educational Facilities
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• v.10 no.3
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• pp.15-26
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• 2003

The purpose of this study is to describe the planning and applied safety facility layout for a technology laboratory in a middle school. A technological teaching facility has its essential purpose in meeting students' needs and achieving educational objectives efficiently and effectively. Practice-related appliances were selected through investigation of the current middle school laboratory, and analysis of the technology textbooks of the 7th curriculum. Laboratory layout was achieved with consideration of the teacher's and students need of safety and close proximity to laboratory facilities and equipment. Our recommendation takes the SLP survey results into consideration. The results of this study are as follows: The following floor plan is recommended. The facility layout will insure that students are able to work safely, in full view of academic instructors. Students will have easy access to all equipment due to the efficient floor plan and logical sequencing of equipment.

• Nuclear Engineering and Technology
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• v.53 no.3
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• pp.793-803
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• 2021

The small PWR has been paid more and more attention due to its diversity of application and flexibility in the site selection. However, the large core power density, the small containment space and the rapid accident progress characteristics make it difficult to control the containment pressure like the traditional PWR during the LOCA. The pressure suppression system has been used by the BWR since the early design, which is a suitable technique that can be applied to the small PWR. Since the configuration and operating conditions are different from the BWR, the pressure suppression system should be redesigned for the small PWR. Conducting the experiments on the scale down test facility is a good choice to reproduce the prototypical phenomena in the test facility, which is both economical and reasonable. A systematic scaling method referring to the H2TS method was proposed to determine the geometrical and thermohydraulic parameters of the pressure suppression containment response test facility for the small PWR conceptual design. The containment and the pressure suppression system related thermohydraulic phenomena were analyzed with top-down and bottom-up scaling methods. A set of the scaling criteria were obtained, through which the main parameters of the test facility can be determined.

• Korean Journal of Clinical Laboratory Science
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• v.53 no.2
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• pp.199-200
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• 2021

• Korean journal of applied entomology
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• v.60 no.2
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• pp.185-191
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• 2021

A Quarantine Pest Research Facility (QPRF) had been build in Animal and Plant Quarantine Agency and designed to ensure alien pests are safety contained inside the laboratory. Functions of QPRF including insect rearing, research, and physical containment facility were verified to check suitability as a research laboratory using the beet armyworm, Spodoptera exigua. As insect rearing and laboratory facility, S. exigua completed its development for 32.2 days at 25℃. Egg hatching, pupation, and adult eclosion showed 80, 86.6, and 90%. Two pesticides, fluxametamide and hydramethylnon showed high susceptibility against S. exigua larvae by spraying and dipping methods. As physical containment facility, male adults, which were artificially released from laboratory, did not capture in delta trap installed sex pheromone lure during winter season. Based on these results, QPRF will be used for study on alien insect species.

• Proceedings of the Korean Nuclear Society Conference
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• 2004.10a
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• pp.1190-1191
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• 2004

New $4^{th}$ generation synchrotron facility, XFEL, is almost similar to previous $3^{rd}$ generation synchrotron facility in the view of radiological aspects and most important positions are a dump and synchrotron radiation beam line. In this paper, tile radiation protection solutions for them and undulator are suggested and discussed.

• Journal of the Korean Society of Safety
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• v.31 no.5
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• pp.49-53
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• 2016

The goal of this study is to analyze accidents occurred at experimental laboratory and to suggest hierarchical taxonomy applicable to prepare countermeasures reducing the experimental laboratory accidents. Recent 5 years accidents were analyzed and classified according to their primary cause, facility or human. Then in case of facility, the accidents were further classified whether they can be fixed by organization or by individual. In case of human factor, they were classified into physical, chemical, or biological to prepare precise measures. Depending on the adequacy of appropriate practice, several measures were suggested such as; whether to improve training of laboratory workers, or to improve training the system, or to improve or prepare practice substantially. A new taxonomy for laboratory accident was suggested complying other governmental agencies' classification such as KOSHA and KGS. Additionally, two kinds of possibilities were suggested such as possibility of major accident and possibility of disaster which can be defined as laboratory accident causing large scale of harmful consequence to residential area or environment by fire, explosion and/or toxic release of hazardous chemicals and/or microbiology.

• Reproductive and Developmental Biology
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• v.36 no.1
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• pp.79-85
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• 2012

Minipigs are regarded as one of the most important laboratory animal in that anatomical and physiological properties are similar to human and their reproduction efficiency is relatively higher compared to other large animal species. Particularly, several diseases that cannot be mimicked in rodent models are successfully occurred or induced in pig models therefore it has been interested in a valuable model for human diseases. Pigs are also 'standard' species in xenotransplantation research. To maximize experimental outcome using minipigs, establishment and management of proper animal facility, right animal husbandry and control of pathogens are very important. In this review, we summarized several international guidelines related with minipigs published by several companies or governments and discuss optimal conditions for providing informative ideas to the researchers who want to use minipigs in their future studies.

• Nuclear Engineering and Technology
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• v.50 no.6
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• pp.989-995
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• 2018

Argonne National Laboratory of the United States and Kharkov Institute of Physics and Technology (KIPT) of Ukraine have cooperated on the development, design, and construction of a neutron source facility. The facility was constructed at Kharkov, Ukraine, and its commissioning process is underway. The facility will be used for researches, producing medical isotopes, and training young nuclear specialists. The neutron source facility is designed with a provision to include a cryogenically cooled moderator system-a cold neutron source (CNS). This CNS provides low-energy neutrons, which will be used in the scattering experiment and material structures analysis. Cold neutron guides, coated with reflective material for the low-energy neutrons, will be used to transport the cold neutrons to the experimental site. The cold neutron guides would keep the cold neutrons within certain energy and angular space concentrated inside, while most of the gamma rays and high-energy neutrons are not affected by the cold neutron guides. For the KIPT design, the cold neutron guides need to extend several meters outside the main shield of the facility, and curved guides will also be used to remove the gamma and high-energy neutron. The neutron guides should be installed inside a shield structure to ensure an acceptable biological dose in the facility hall. Heavy concrete is the selected shielding material because of its acceptable performance and cost. Shield design analysis was carried out for the CNS guide hall. MCNPX was used as the major computation tool for the design analysis, with neutron and gamma dose calculated separately. Weight windows variance reduction technique was also used in the shield design. The goal of the shield design is to keep the total radiation dose below the $5.0{\mu}Sv/hr$ guideline outside the shield boundary. After a series of iterative MCNPX calculations, the shield configuration and parameters of CNS guide hall were determined and presented in this article.