• Journal of Bio-Environment Control
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• v.22 no.4
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• pp.303-308
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• 2013

A remote controlled sprayer has designed, manufactured and experimented to spray well on pear trees with pesticides. This study was executed to automate pest management of pear trees. Types of spray nozzle, which was used on the system, were analyzed experimentally to find an optimal spray equipment configuration with several nozzles. Attributions of ultrasonic sensors were analyzed to adjust spraying distance of an unmanned sprayer system. This paper investigated shapes of pear trees and cultivating environment of pear orchard. In order to select optimal spray environment, liquid distribution was measured while angle of nozzle was changed. Additionally, liquid distribution by distance and sprayed liquid capacity by side distance were measured. According to information of shapes of pear trees and cultivating environment of pear orchard, sprayer frames of an unmanned sprayer system were manufactured and sprayer frames were suitable for interval of pear trees. The sprayer system could adjust width of sprayer frames to 2.5 m and height of sprayer frames to 1.7 m. Optimal angle of nozzle, and optimal distance between objects and nozzle were $15^{\circ}$ and 0.8 m. When side distance was placed from 1.2 m to 1.8 m, sprayed capacity reached to the highest amount.

• Journal of Korean Society for Geospatial Information Science
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• v.24 no.4
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• pp.47-58
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• 2016

The purpose of this study was to select the sectoral vulnerable areas index in welfare field and analyze the socially vulnerable areas from comprehensive analysis. For this study, preceding research and theoretical background were reviewed. Through this, we selected for the analysis index and criteria, and data corresponding to the index are collected. Based on the index and criteria, the data analysis was performed in Seoul Metropolitan City selected as the spatial extent of this study. From the results of analysis, the sectoral lower rank 10% of social vulnerable areas was determined. In addition, Junggu, Yongsangu, and Seodaemungu from the comprehensive analysis of individual vulnerable areas were derived as a final vulnerable areas. In particular, Junggu was weak in all sectors; Yongsangu was in the medical sector; and Seodaemungu was poor in housing and education. Lower vulnerability index of all sectors (energy, housing, medical, transportation, and education) in 1st, 2nd, and 3rd residential areas by examining use zoning was showed. From the results of this study, we can expect time and labor saving of policy support in public sector.

• Food Science of Animal Resources
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• v.35 no.6
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• pp.765-771
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• 2015

The development of a sample preparation method and optimization of the analytical instrumentation conditions were performed for the determination of the vitamin B₁₂ content in emulsified baby foods sold on the Korea market. After removal of the milk protein and fats by chloroform extraction and centrifugation, the vitamin B₁₂ was water extracted from the sample. Following filtration of the solution through a nylon filter, the water-soluble extract was purified by solid-phase extraction using a Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS). The solution eluted from the cartridge was dried under a stream of nitrogen gas and reconstituted with 1 mL of water. The sample solution was injected into an LC-MS/MS system after optimizing the mobile phase for vitamin B₁₂ detection. The calibration curve showed good linearity with the coefficient of correlation (r²) value of 0.9999. The limit of detection was 0.03 µg/L and the limit of quantitation was 0.1 µg/L. The method of detection limit was 0.02 µg/kg. The vitamin B₁₂ recovery from a spiking test was 99.62% for infant formula and 99.46% for cereal-based baby food. The sample preparation method developed in this study would be appropriate for the rapid determination of the vitamin B₁₂ content in infant formula and baby foods with emulsified milk characteristics. The ability to obtain stable results more quickly and efficiently would also allow governments to exercise a more extensive quality control inspection and monitoring of products expected to contain vitamin B₁₂. This method could be implemented in laboratories that require time and labor saving.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.11
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• pp.1886-1891
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• 2013

To inactivate the microorganisms on the surface of blueberries after harvest and to secure microbial safety, a bubble-aqueous chlorine dioxide washing system was developed. After treating the freshly prepared blueberries with the bubble-aqueous chlorine dioxide washing system, the changes in the microbial populations and quality of the blueberries were determined during storage $4{\pm}1^{\circ}C$. Microbiological data indicated that the populations of total aerobic bacteria, yeast and mold decreased by 1.4 and 1.3 log CFU/g at the treatment of 20 ppm aqueous chlorine dioxide with the system, respectively. There was no significant difference in color change and weight loss during storage among treatments. In addition, this washing system could handle approximately 60 kg of blueberry per hour, resulting in labor-saving. Therefore, these results clearly suggest that the bubble-aqueous chlorine dioxide washing system could be useful in improving the microbiological safety of fresh blueberries after harvest.

• Korean Journal of Microbiology
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• v.47 no.1
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• pp.87-91
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• 2011

The aim of this study was to compare the performance of conventional culture and real-time PCR for detection of Cronobacter spp. in powdered foods. Infant formula, baby food and Misugaru inoculated with Cronobacter were enriched in distilled water as first enrichment step, followed by incubating in Enterobacteriaceae enrichment (EE) broth as second enrichment step. A loopful of enriched sample was streaked onto Druggan-Forsythe-Iversen agar, followed by incubating at $37^{\circ}C$ for 24 h. One milliliter of the enriched distilled water and EE broth were used in real-time PCR assay. No statistical differences were observed in the number of positive samples between culture method and real-time PCR (p>0.05) in all types of food samples. The number of positives of real-time PCR was higher in the first enrichment media (distilled water) than the second enrichment media (EE broth), though there was no significant difference (p>0.05). It appears that some components of the second enrichment broth, EE broth, inhibit the reaction of real-time PCR. These results show that real-time PCR using a single enrichment with distilled water could be useful as an effective screening method for detection of Cronobacter while saving much time and labor compared to conventional culture method.

• Korean Journal of Plant Resources
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• v.13 no.1
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• pp.41-47
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• 2000

This study was conducted to evaluate development of seed pellet technique such as pellet polymer search, the shape formation and hardness, the germination and emergence rate of the pellet seeds for labor-saving and reducing production cost in onion(Allium cepa L.) cultivation. The pellet seeds shape formation was good such as kaolin, clay, ash, and gypsum, and clay was good shape formation but surface of pellet seed was cracked during the drying. PG(pearlite + gypsum) as pellet material and PVA as binder were the best among the material in consideration with shape and hardness together. The hardness of the pellet seeds was affected by polymers, the kinds and concentration of binders, and that degree was large at polymer. The high hardness polymers were gypsum and coal ash, but burned lime was the lowest hardness among the pellet material. The germination(GP) and emergence percentage(EP) of pellet seed with PG in vitro were the highest among the material, and that was 93.6, 91.8%, respectively. The EP of pellet seed with PG at 20, $25^{\circ}C$ were 91.3, 92.0%, respectively, The EP of pellet seed were over the 91%, and those did not show difference with field moisture capacity , and that of 5 and 6mm size seed were the highest as 92%, respectively. and other size seeds showed over 90%, too. The EP of pellet seed with PG was decreasing as increasing the sowing depth, and that of at 10mm sowing depth was the highest as 92.7%. The time to 50% emergence of that under 70% field moisture capacity was 158h, and that was delayed at 20h compare to control seed.

• Journal of Bio-Environment Control
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• v.20 no.1
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• pp.33-39
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• 2011

This experiment was carried out to understand the characteristics of inorganic ion uptake of strawberries to establish a labor saving, environmentally sound closed hydroponic system. Nitrate nitrogen is absorbed in the perlite and granule rockwood with the almost same rate and in the cocopeat with a little bit higher concentration. At the early growth stage, phosphate was absorbed in comparatively high rate, but it is gradually declining to $4me{\cdot}L^{-1}$ in the three treatments except for reused medium. Four treatments showed different absorption rates for potassium, respectively, however, it became $3{\sim}5me{\cdot}L^{-1}$ gradually. But there was few sign to absorb potassium in the reused medium. High concentration of calcium was absorbed in the cocopeat medium and the lowest absorption rate in the granule rockwool, and it turned out to be $2{\sim}3me{\cdot}L^{-1}$ absorption concentration rate in the perlite. All three treatments showed $1me{\cdot}L^{-1}$ at the average absorption concentration rate of magnesium. Iron showed high absorption rate but it showed steadily 1~2 ppm absorption rate. Results of this experiment will be utilized for developing the new substrates for strawberry closed hydroponic system.

• Journal of Bio-Environment Control
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• v.17 no.1
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• pp.60-65
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• 2008

For autumn field cutting of Sedum sarmentosum, the effects of cutting site (distal, middle, and proximal) and planting method (space drill seeding, drill seeding, and broadcast seeding) on survival rate and growth characteristics were investigated at pre- and post-winter season. Plant height root length, stem number per plant, number of branch per plant, fresh weight of shoot, and dry weight of shoot were significantly superior in distal site at pre-wintering (40 days after cutting). At post-wintering (May 10th), stem number per plant, fresh weight and dry weight per $m^2$ in cutting of distal site showed a significant increasement compared to the cutting of proximal site. At pre-wintering (40 days after cutting), the growth in space drill seeding was well than that in drill seeding and broadcast seeding, and fresh weight and dry weight per $m^2$ in space drill seeding were high in order of space drill seeding, broadcast seeding, and drill seeding. At post-wintering (May 10th), stem number per plant, fresh weight and dry weight per $m^2$ in space drill seeding were significantly increased than those in drill seeding. Accordingly, the cutting using distal site of stem in autumn field cutting was desirable for the growth and shoot yield. The space drill seeding showed the highest yield potential among three seeding methods, but broadcast seeding was favorable in saving of labor, because the fresh weight of shoot in broadcast seeding was similar in the space drill seeding at post-wintering.

• Journal of Bio-Environment Control
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• v.23 no.3
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• pp.256-261
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• 2014

This study was carried out to determine the effect of the artificial pollination on fruit set, seed formation and fruit quality in kiwifruit and to confirm the effective pollination time for optimal fruit set in the shelter greenhouse cultivation. Also, the effect of artificial pollination practice by the pollen diluent solution was tested. By artificial pollination, fruit set, seed formation, and fruit quality was improved both in the open field condition and in the shelter greenhouse cultivation. In order to find the effective pollination period (EPP), artificial pollination was carried out every day until 7 days after full bloom. Number of seeds and fruit set rate and fruit growth of kiwifruit were no significantly different until 4 days after full bloom (DAFB), but, those of kiwifruit from 5 DAFB significantly decreased. Consequently, the effective pollination period of kiwifruit in the shelter greenhouse was 4 days after full bloom. Also, artificial pollination by the pollen diluent solution was showed the similar fruit set and fruit quality compare to that by lycopodium powder. Accordingly, artificial pollination by the pollen diluent solution should be very effective practice for labor saving in kiwifruit cultivation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.2
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• pp.157-167
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• 1996

The method of direct seeding on flooded paddy surface in rice is known to be the most labor saving cultural practice in rice. However, this method has a problem in practical use such as severe lodging occurring at the reproductive growth stage. The objectives of this study were observated degree of field lodging and variation of lodging-related characteristics with different seeding dates. The number of seedlings per m$^2$ were from 91 to 144 plants, and seedling ratios were from ranged from 61.7% to 91.8%. Days from seeding to flowering were shortened from 5 to 15 days by the later seeding dates. Heading dates of Ilpumbyeo, Seoanbyeo, Daecheongbyeo and Donjinbyeo on June 9 showed slightly elapsed on the critical stable heading time from, August 28 to 29. The culm length was effective in longer clum varieties than semidwarf varieties. The degree field lodging (degree of lodging: 0~9< 9 : complete lodging) in Obongbyeo and S101 with semidwarf varieties were 0.17, whereas 1.25 in Dongjinbyeo with long culm. The lodging resistant varieties and later seeding dates shortened the length of fibrous in the clum, and thickened fibrous in the clum, respectively. Obongbyeo and Sl0l showed stronger resistance to field lodging. The lodging resistant varieties, Obongbyeo and Sl0l, showed lower values of lodging index from 1.03 to 1.15 than those of lodging susceptible varieties, Daecheongbyeo and Palgongbyeo ranged from 1.42 to 1.70. Bending moment with leaf sheath were greater in lodging resistant varieties (Obongbyeo and Sl0l),1510.0~1930.4g.cm, than those in the lodging susceptible varieties (Daecheongbyeo and Palgongbyeo), 1127.2~1287.6g.cm.