• Title/Summary/Keyword: Lab kit

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Evaluation of Two ELISA and Two Indirect Hemagglutination Tests for Serodiagnosis of Pulmonary Hydatid Disease

  • Eris, Fatma Nur;Akisu, Ciler;Aksoy, Umit
    • Parasites, Hosts and Diseases
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    • v.47 no.4
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    • pp.427-429
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    • 2009
  • To establish a definite diagnosis for pulmonary hydatid disease, combination of radiology and serology is useful. In this study, 19 preoperative sera from patients with surgically confirmed pulmonary hydatidosis, 40 sera from patients with other parasitosis and pulmonary diseases, and 20 sera from healthy donors were evaluated using 4 different serological tests, i.e., the commercial ELISA (ELISA-kit) test, the ELISA (ELISA-lab) test prepared in our laboratory, the commercial indirect hemagglutination assay kit (IHA-kit) test, and the IHA test using sensitized sheep red blood cells with tannic acid (IHA-TA). The ELISA-kit was the most sensitive (84.2%) and the most specific test (100.0%). The ELISA-kit also demonstrated the highest positive (100.0%) and negative (95.2%) predictive values. The sensitivity of the ELISA-lab test, that we prepared, was found to be 73.6%, whereas the IHA-kit test and the IHA-TA test were found to be 73.6% and 68.4%, respectively. The specificity of these tests was 96.6%, 98.3%, and 83.3%, respectively. When all 4 tests were assessed together, it was found that the sensitivity had risen to 94.7%. When the ELISA-kit was assessed with the IHA-kit and IHA-TA together, it was found that the sensitivity was 89.5% and 84.2%, respectively. Likewise, the combination of the ELISA-lab and IHA-kit or IHA-TA allowed us to achieve a sensitivity of 84.2% in cases of pulmonary echinococcosis. In conclusion, the diagnosis would be imminent if least 2 tests were applied together.

Studies on Development of Microplate-EIA for the Determination of Serum Progesterone (혈청 Progesterone 측정을 위한 효소면역분석법 개발에 관한 연구)

  • 김정우;이욱연
    • Korean Journal of Animal Reproduction
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    • v.17 no.4
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    • pp.347-356
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    • 1994
  • A simpled and sensitive microplate enzyme immunoassay(EIA) was developed for the determination of progesterone concentration in serum, based on progesterone monoclonal antibody as anti-progesterone, horseradish peroxidase(HRP) as enzyme-label and tetramethylbenzidine(TMB) as substrate. The assay has a sensitivity of 5 pg-120pg/well and intra- and inter-assay coefficients of variation for progesterone standard curve (1.0ng~10.0ng/ml) were ranged 2.5~9.9% and 1.7.8.0%, respectively, determination coefficient of the regressio equation of our standard curve(R2=0.990$\pm$0.007) were high, and this is the same level as that of commercial kit(Hormonost Bio-Lab, Germany, R2=0.98~0.99). The progesterone concentration of serum determined by both kits (Work & Bio-Lab) were significantly correlated (r=0.95, P<0.01) although a little higher value were resulted in our kit than that of commercial kit. It generally is these results indicated that the microplate-EIA can be cused for the determination of progesterone in serum, as well as, for the determination of the early pregnancy diagnosis.

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Developing an IT Course Utilizing Raspberry Pi (라즈베리파이를 활용한 IT 교과목 개발)

  • Yoo, Hyeon-Joong
    • Journal of Practical Engineering Education
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    • v.7 no.2
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    • pp.89-95
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    • 2015
  • IT technologies are closely related to our daily life, and we are experiencing the emergence of new IT devices every day. The ongoing rapid progress of IT technology results in the increasing demand of experienced software & hardware engineers, and leads to the short life cycle of lab kits. However, the normally small to medium sized manufacturers of lab kits usually cannot even afford to support timely update and upgrade of their own kits. In this paper, we propose a way of effectively running an IT course by building a Raspberry Pi based kit. Raspberry Pi has features that allow us to not only timely update/upgrade the kit, but also expose students to various IT fields.

Development of Transformable Reflective Telescope Kit Using Aluminum Profile and Isogrid Struture

  • Lee, Sumin;Park, Woojin;Lee, Sunwoo;Han, Jimin;Ann, Hojae;Ji, Tae Geun;Kim, Dohoon;Kim, Ilhoon;Kim, Junghyun;Pak, Soojong
    • The Bulletin of The Korean Astronomical Society
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    • v.46 no.1
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    • pp.38.1-38.1
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    • 2021
  • 본 발표에서는 Transformable Reflective Telescope(TRT kit)의 새로운 버전을 소개한다. TRT kit는 기본형인 뉴턴식 반사망원경에서 부경 교체를 통해 카세그레인식, 그레고리식으로 간단하게 변형 할 수 있는 광학 실험장치이다. 본 장치는 주로 망원경 교육이나 광학계 개발에 필요한 실험에 활용된다. 모듈화 설계를 통해서 여러 종류의 광학계를 쉽게 탈착하여 다양한 실험을 할 수 있다. 광기계부는 정밀하게 제작된 알루미늄 프로파일과 Isogrid구조를 채택하여 경량화 구조로 설계되었다. 이러한 경량부품들을 통해 이전 버전보다 50~70%의 중량 감소율을 달성하였다. 유한요소해석 결과 경량화된 뉴턴식 TRT kit는 이전 버전과 비교해서 자체 하중에 의한 최대 구조 변형이 0.11mm에서 0.023mm로 감소하였다. 부경 지지대 설계에는 자체 하중으로 인한 변형을 최소화하기 위해 트러스 (Truss) 구조가 도입되었다. 부경부의 자체 하중으로 인한 변형은 기존의 80㎛에서 21㎛로 감소하였다. 또한, 십자 레이저 정렬 장치가 추가되어 뉴턴식과 카세그레인식에서 공차 1.5' 이내로 광학계 정렬이 가능하다.

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Implementation and Experiment of Neural Network Controllers for Intelligent Control System Education

  • Lee, Geun-Hyeong;Noh, Jin-Seok;Jung, Seul
    • International Journal of Fuzzy Logic and Intelligent Systems
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    • v.7 no.4
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    • pp.267-273
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    • 2007
  • This paper presents the implementation of an educational kit for intelligent system control education. Neural network control algorithms are presented and control hardware is embedded to control the inverted pendulum system. The RBF network and the MLP network are implemented and embedded on the DSP 2812 chip and other necessary functions are embedded on an FPGA chip. Experimental studies are conducted to compare performances of two neural control methods. The intelligent control educational kit(ICEK) is implemented with the inverted pendulum system whose movements of the cart is limited by space. Experimental results show that the neural controllers can manage to control both the angle and the position of the inverted pendulum systems within a limited distance. Performances of the RCT and the FEL control method are compared as well.

The selection of basic platform for improving the sensitivity of neutravidin rapid detection kit (뉴트라비딘 검출용 간이 진단키트의 성능향상을 위한 기본 플랫폼 선정)

  • Choi, Sunmi;Kim, Giyoung;Om, Aeson;Moon, Jihea;Park, Saetbyeol;Lee, Sangdae;Kim, Hyuk Joo
    • Korean Journal of Agricultural Science
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    • v.39 no.4
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    • pp.613-618
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    • 2012
  • This study was performed to optimize the basic platform of a lateral flow immunoassay. Improvement of the limit of detection (LOD) was evaluated according to the width of a nitrocellulose membrane with varying concentrations of analyte. The analyte, neutravidin was detected based on the avidin-biotin interaction. The antibody-Au nanoparticle conjugation was mostly stabled in a PBS buffer of pH 7.3. The optimal widths of a nitrocellulose membrane were 4 and 6 mm considering the sample flow rate and signal strength of the test line on the membrane. The LOD of neutravidin was 0.001 mg/ml in the optimum conditions.

Neuro-Fuzzy Control of Inverted Pendulum System for Intelligent Control Education

  • Lee, Geun-Hyung;Jung, Seul
    • International Journal of Fuzzy Logic and Intelligent Systems
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    • v.9 no.4
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    • pp.309-314
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    • 2009
  • This paper presents implementation of the adaptive neuro-fuzzy control method. Control performance of the adaptive neuro-fuzzy control method for a popular inverted pendulum system is evaluated. The inverted pendulum system is designed and built as an education kit for educational purpose for engineering students. The educational kit is specially used for intelligent control education. Control purpose is to satisfy balancing angle and desired trajectory tracking performance. The adaptive neuro-fuzzy controller has the Takagi-Sugeno(T-S) fuzzy structure. Back-propagation algorithm is used for updating weights in the fuzzy control. Control performances of the inverted pendulum system by PID control method and the adaptive neuro-fuzzy control method are compared. Control hardware of a DSP 2812 board is used to achieve the real-time control performance. Experimental studies are conducted to show successful control performances of the inverted pendulum system by the adaptive neuro-fuzzy control method.

Assay of Human Chorionic Gonadotropin in Urine of Athletes and Evaluation of Assay Kit Performance (운동선수들의 뇨 중 Human Chorionic Gonadotropin 분석 및 분석킷트 평가)

  • 최명자;이정란;김명수
    • Biomolecules & Therapeutics
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    • v.10 no.3
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    • pp.186-192
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    • 2002
  • Special attention has been paid to human chorionic gonadotropin (hCG) for athlete doping control because it stimulates the endogenous production of testosterone and epitestosterone without increasing the T/E ratio which is a doping indicator for the exogenous administration of testosterone. Even though the IOC banned the use of hCG, a detection method has not been decided upon since there are a variety of immunoassay kits available on the market. We evaluated three kits in terms of their performance characteristics. The assay value of the control sample varied depending on the kit, resulting in 198 mIU/ml for the MAIA kit, 172mIU/ml for the IRMA kit, and 143 mIU/ml for the MEIA kit. Considering the IOC inter-lab distribution of results(55-312 mIU/ml) using 27 different kits and the mean value (178$\pm$56 mIU/ml), all three kits are within the range of -15.8% - +5.6% of the mean value, which proves them useful for the hCG assay. The MEIA kit resulted in lower hCG values because it detects only intact hCG molecules, in contrast to the other two kits which detect intact hCG and -hCG together. However, it is suitable for screening purposes because of its advantage of being an automated system. When 123 urine samples of athletes were analyzed in 22 batches using this system, the variation of control values fell within $\pm$ 10% of the mean values, and an specimens tested negative with hCG values less than the detection limit of 2 mIU/ml.

A STUDY ON THE GLOSS AND ROUGHNESS OF THE COMPOSITE RESIN (복합레진의 광택 및 표면조도에 관한 연구)

  • Cho, Seung-Joo;Lee, Myung-Jong
    • Restorative Dentistry and Endodontics
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    • v.15 no.1
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    • pp.67-80
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    • 1990
  • This study was performed for elucidating the effects on surface polishing of composite resins. In this study, Silux(microfilled), Graft(hybrid), Bisfil- I (hybrid posterior) and Hi-pol(conventional) were used. Sixty specimens were made with 4 brands of composite resins and Optilux system in $2.0{\times}1.3{\times}1.0cm$ resin block which has a cavity with 0.5cm diameter and 0.5cm depth. Polishing was done with #600 sand paper and Soflex, Super-snap, Micron finishing system, or Composite polishing kit. Final polished surfaces were measured by roughness tester(Kasaka Lab. Ltd., Japan) and image analyser(Omnimet Image Analyser, Buehler, USA). The results were as follows, 1. The celluloid strip produced the smoothest surfaces. 2. Light curing microfilled composite resin, Silux, had smoother surface than any others. 3. The surfaces polished by Soflex were smoothest. 4. Aluminum oxide disk, Soflex and Super-Snap, made smoother surface than diamond bur, M.F.S., or silicon point, Composite polishing kit. 5. The roughness values of surface polished by M.F.S. composed of diamond burs, were less than those of Composite polishing kit made from silicone points.

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