• Title/Summary/Keyword: LU10

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Nested PCR을 이용한 사람 유래 태아 폐세포에서 배양된 Rickettsia tsutsugamushi의 혈청형 동정 (Use of Nested Polymerase Chain Reaction for Identification of Rickettsia tsutsugamushi Serotype Cultured in Human Embryonic Lung Cells)

  • 안창남;우규진;김태연;신광순;김철중;백락주
    • 대한바이러스학회지
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    • 제26권2호
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    • pp.235-244
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    • 1996
  • Rickettsia tsutsugamushi의 원형균주인 Karp주와 Gilliam주를 초대 배양된 사람 정상 2배체 폐세포(LuMA cell)를 이용하여 증식과 세포병변들의 속도를 비교할 수 있었고, 배양된 균주는 네스티드 프라이머를 사용하여 혈청형을 동정할 수 있었다. R. tsutsugamushi의 세포벽 외막에 존재하며 혈청형을 결정하는 주요항원은 54-56Kd 단백인 것으로 밝혀지고 있는데, 이 단백 유전자의 DNA 염기서열을 분석하여 Karp주와 Gilliam주의 공통서열로 첫번째 프라이머쌍을 만들었고 첫번째 프라이머쌍의 안쪽에 위치한 혈청형 사이에 차이가 있는 서열로 두번째 프라이머쌍을 만들었다. 네스티드 뉴클레오티드 프라이머는 중합효소 연쇄반응의 특이성을 증가시킬 수 있는데 이 실험 결과로 이 PCR 방법은 scrub typhus의 진단과 혈청형의 동정에 적용될 수 있을 것으로 보여진다.

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수근관절염좌 환자에 대한 아시혈 치료 (Ashi Points-acupuncture for Wrist Sprain)

  • 강태경;김명동
    • 동의생리병리학회지
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    • 제29권4호
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    • pp.337-346
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    • 2015
  • Sprain is the injury of meridian-muscle, and is caused by qi and blood obstruction or regional stagnation of qi and blood. So we take the channel points where pain flows. If we take the locations that feel pain, those locations are treatments points and ashi points. So we searched over the ashi points appearing on the patients with wrist sprain. Ashi points appeared on LI5, TE4, SI5 around wrist joints, LI10, LI11, LU6 around elbow joints, LI14, LU3, LU4, PC2 around upper arm. Also, ashi points appeared much on ST17, KI23, PC1, SP18, ST18 around thoracic region, and, on BL15, BL44, BL13 around anterior and thoracodorsal region, in order stated. Ashi points of the highest frequency appeared on LI14 around upper arm, and on LI5, TE4 around wrist joint, and SI5, ST17, KI12, PC1, SP18 appeared with second highest frequency. And ashi points on elbow points and thoracodorsal region appeared with the same frequency. Therefore, it is possible for us to know that the pain location appears in order of upper arm, anterior thoracic region, elbow joint region, and, thoracodorsal region, in treating wrist joints. There was a tendency that pain and movement disturbance recovered more quickly, depending on the pain reduction, as we found out the ashi points closely from stagnated qi and blood caused by wrist arthritis, and relaxed the stiff location. Rubbing treatments in treating pain ashi points is considered to play an important role to reduce pain effectively, so it is necessary to make a further study.

사암도인침법에서 태백혈의 운용에 대한 연구 (A Study on Acupoint SP3 in Saam Acupuncture Method)

  • 김송이;이순호;박지연;박히준
    • Korean Journal of Acupuncture
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    • 제31권4호
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    • pp.179-187
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    • 2014
  • Objectives : Saam acupuncture initiated by Saamdoin is traditional and originative method, which is characterized by applying the five phases theory and mother-child reinforcement-reduction principle to the selection of acupoints and needling manipulation. Our study was aimed to summarize and assess the use of acupoint SP3(Taebaek) in Saam acupuncture treatment and to further understand Saam acupuncture in an aspect of the combination of acupoints. Methods : We searched the data based on "(Do Hae Kyo Kam) Sa-Ahm's acupuncture method" for SP3 used and acupoint combination including SP3. We performed frequency analysis, network analysis, and cluster analysis for quantitative aspect. To understand clinical implication of SP3 with another acupoint, qualitative and descriptive methods were also carried out. Results and Conclusions : In our study, SP3 was frequently used for tonification of lung, spleen, heart, and kidney meridian and sedation of kidney, heart, and lung meridian. For this, many acupoints such as LU8, LU9, KI3, HT8, KI7, LU10 and LR1 were used with SP3. The combination of SP3 and other acupoints were used to treat stroke, common cold, and pain conditions including headache, low back pain, respiratory disease as well as gastroenteric troubles including stomachache, indigestion, vomiting, and constipation. To further understand Saam acupuncture, an understanding of the five transport points based on five elements characters, pathological changes (deficiency and excess) of viscera and bowels, and concept of source point should be preceded.

An HPLC-UV-based quantitative analytical method for Chrysanthemum morifolium: development, validation, and application

  • Jung, Dasom;Jin, Yan;Kang, Seulgi;Lee, Heesoo;Park, Keunbae;Li, Ke;Kim, Jin Hak;Geum, Jeong Ho;Lee, Jeongmi
    • 분석과학
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    • 제32권4호
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    • pp.139-146
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    • 2019
  • A simple and reliable analytical method based on high-performance liquid chromatography-ultraviolet detection was established for the analysis of the flowers of Chrysanthemum morifolium (CM). Luteolin-7-O-glucoside (LU7G) was chosen as a target analyte considering its content, availability, and ease of analysis. Chromatographic separation of LU7G was achieved using a Phenomenex Gemini $C_{18}$ column ($250{\times}4.6mm$, $5{\mu}m$) run with a mobile phase consisting of 0.5 % acetic acid in water and 0.5 % acetic acid in acetonitrile at a flow rate of $1.0mL\;min^{-1}$. The detection wavelength and column temperature were set at 350 nm and $40^{\circ}C$, respectively. Method validation was performed according to the AOAC guidelines and the method was specific, linear ($R^2=0.9991$ for $50-300{\mu}g\;mL^{-1}$), precise (${\leq}3.91%$RSD), and accurate (100.1-105.7 %). The limits of detection and quantification were 3.62 and $10.96{\mu}g\;mL^{-1}$, respectively. The established method was successfully applied to determine the contents of LU7G in various batches of bulk CM extracts and labscale CM extract. The developed method is a readily applicable method for the quality assessment of CM and its related products.

충남 태안 철마산 일대의 지질 및 희토류 광화작용 (REE Mineralization and Geology of Chulmasan Area, Taean, Chungchungnamdo)

  • 유봉철
    • 한국광물학회지
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    • 제32권2호
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    • pp.127-143
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    • 2019
  • 철마산 일대의 지질은 하부로부터 선캠브리아기의 소근리층, 화강암질편마암, 엽리상 흑운모화강암, 엽리상 운모화강암, 염기성암맥 및 산성암맥으로 구성된다. 이 일대의 희토류 광화작용은 화강암질편마암과 엽리상 운모화강암에서 관찰된다. 이들 암석에서 소량 희토류 원소 및 토륨을 함유한 광물들은 저어콘($Y_2O_3$ 0.00~1.18 wt.%, $Gd_2O_3$ 0.00~0.59 wt.%, $Er_2O_3$ 0.00~0.22 wt.%, $Yb_2O_3$ 0.00~0.34 wt.%, $Lu_2O_3$ 0.00~0.48 wt.%, $ThO_2$ 0.00~0.33 wt.%), 토리아나이트($Nd_2O_3$ 0.00~0.24 wt.%, $Lu_2O_3$ 0.00~0.26 wt.%), 베르시에린($La_2O_3$ 0.04~0.26 wt.%, $Nd_2O_3$ 0.00~0.20 wt.%, $Tb_2O_3$ 0.04~0.12 wt.%, $Dy_2O_3$ 0.17~0.26 wt.%, $Er_2O_3$ 0.33~0.44 wt.%, $Lu_2O_3$ 0.00~0.19 wt.%, $ThO_2$ 0.61~0.93 wt.%), 녹니석($La_2O_3$ 0.44~0.68 wt.%, $Ce_2O_3$ 0.12~0.13 wt.%, $Nd_2O_3$ 0.31~0.44 wt.%, $Eu_2O_3$ 0.03~0.08 wt.%, $Dy_2O_3$ 0.09~0.21 wt.%, $Ho_2O_3$ 0.04~0.14 wt.%, $Er_2O_3$ 0.18~0.32 wt.%, $Lu_2O_3$ 0.07~0.21 wt.%, $ThO_2$ 0.00~0.97 wt.%), 흑운모($Nd_2O_3$ 0.02~0.08 wt.%, $Gd_2O_3$ 0.07~0.08 wt.%, $Tb_2O_3$ 0.02~0.07 wt.%, $Dy_2O_3$ 0.35~0.43 wt.%, $Ho_2O_3$ 0.15~0.26 wt.%, $Er_2O_3$ 0.24~0.28 wt.%, $Yb_2O_3$ 0.06~0.18 wt.%, $ThO_2$ 0.00~0.12 wt.%), 정장석($Dy_2O_3$ 0.05~0.12 wt.%, $Ho_2O_3$ 0.05~0.06 wt.%, $Er_2O_3$ 0.28 wt.%, $Yb_2O_3$ 0.06~0.12 wt.%) 및 사장석($Ho_2O_3$ 0.01~0.03 wt.%, $Er_2O_3$ 0.10~0.27 wt.%, $ThO_2$ 0.11~0.13 wt.%)이며 희토류 광물로는 바스트나사이트와 퍼구소나이트이다. 희토류 광물들은 주로 장석류, 운모류, 저어콘, 인회석 및 티탄철석의 간극을 따라 산출된다. 따라서 철마산 일대의 희토류 광화작용 산물인 바스트나사이트와 퍼구소나이트는 화강암질편마암과 엽리상 운모화강암의 형성 시 희토류 원소 및 토륨이 구성광물 내에 소량 함유되어 있었으며 그후 계속된 화성활동 및 변성작용에 의하여 기존 광물 내에 함유되어 있던 희토류 원소가 재 농집에 의해 형성된 것으로 생각된다.

Investigation and Simulation Study on the Cascading Trip-off Fault of a Large Number of Wind Turbines in China on May 14, 2012

  • Qiao, Ying;Lu, Zong-Xiang;Lu, Ji;Ruan, Jia-Yang;Wu, Lin-lin
    • Journal of Electrical Engineering and Technology
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    • 제10권6호
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    • pp.2240-2248
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    • 2015
  • The integration of the large-scale wind power brings great challenge to the stability of the power grid. This paper investigates and studies the fault on May 14, 2012 of the large-scale cascading trip-off of wind turbines in North China. According to the characteristics of the voltage variation, the fault process is divided into three stages: the pre-event stage, the critical stage before cascading, and the cascading stage. The scenes in the fault are reproduced, using the full-size actual power system model. Simulation models of double-fed induction generators (DFIGs) and SVCs including protection settings and controller strategies are carefully chosen to find out the reason of voltage instability in each stage. Some voltage dynamic that have never been observed before in the faults of the same kind are analyzed in detail, and an equivalent voltage sensitive dynamic model of DFIG is proposed for the fast computation. The conclusions about the voltage dynamics are validated by the actual PMU observation evidence.

Advances in Enhancing Nutrient Utilization for Ruminants by Nutritional Manipulation to Reduce Environmental Contamination - Review -

  • Lu, De-xun
    • Asian-Australasian Journal of Animal Sciences
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    • 제14권3호
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    • pp.395-401
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    • 2001
  • A review of present understanding of the dietary nutrient utilization in ruminant animals is presented. For increasing the utilization of dietary nutrients and reducing environmental contamination, highlighting the development and use of nutritional manipulation technique is suggested.

MIXED CHORD-INTEGRALS OF STAR BODIES

  • Fenghong, Lu
    • 대한수학회지
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    • 제47권2호
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    • pp.277-288
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    • 2010
  • The mixed chord-integrals are defined. The Fenchel-Aleksandrov inequality and a general isoperimetric inequality for the mixed chordintegrals are established. Furthermore, the dual general Bieberbach inequality is presented. As an application of the dual form, a Brunn-Minkowski type inequality for mixed intersection bodies is given.