• Animal cells and systems
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• 제13권3호
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• pp.297-304
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• 2009

A complete mRNA sequence of transferrin from the wax moth, Galleria mellonella, was obtained, and compared with those of other species. We previously reported that the sequence was most similar to those of Manduca sexta and Bombyx mori. As in other moths, G. mellonella transferrin had only one iron-binding site at its N-terminal region. Semi-qRT PCR was conducted to investigate tissue-specific distribution and transcriptional regulation of the wax moth transferrin mRNA. Larval muscle and fat body contained larger quantity of mRNA than other tested tissues. In this study, it was observed that iron and cadmium regulated transferrin transcription, and this regulation pattern was tissue specific. Iron up-regulated transferrin mRNA level in fat body, while suppressed it in the Malpighian tubules and silk glands. Cadmium decreased the mRNA level in fat body, muscle, and Malpighian tubules, but significantly increased the mRNA level in silk glands. In addition, the mRNA expression was induced by all tested pathogen-associated molecular patterns (PAMPs) including LPS, lipoteichoic acid (LTA), glucan, and even chitin.

• 한국가금학회:학술대회논문집
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• 한국가금학회 2006년도 제23차 정기총회 및 학술발표회
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• pp.84-85
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• 2006

This study was carried out to ascertain phylogenetic status of long-tail chicken which found recently in Korea and was presumed to be a kind of Korean Natives. 10 loci microsatellites were analysed for 449 birds of 11 groups and 2 region of mitochondrial DNA were sequenced for 135 birds of the same groups, that consist of 3 introduced breeds and 8 Korean Natives including 3 long-tail chicken. In mean numbers of alleles per locus(MNA) for microsatellites, long-tail chicken were smaller (2.60${\sim}$3.20) than the others, but in heterozygosities, were higher(0.4087${\sim}$0.5375) than others that were the same level of MNA. And in the neighbor joining bootstrap tree drawing by Nei's standard distance, they made a cluster with some Korean Native groups. All of the nucleotide sequences of mitochondrial cytochrome b gene and D-loop were classified into 23 haplotypes. In long-tail chicken, the haplotypes were 3 kinds, and were different among the groups (LTA, LTB and LTD). Resultly, it was supposed that 3 groups of the long-tail chicken be all a kind of Korean Natives.

• 조명전기설비학회논문지
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• 제29권3호
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• pp.69-80
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• 2015

This paper is writing about developing magnet power supply. It is very important for power supply to obtain output current in high precision and high stability. As a switching noise and a power noise are the cause of disrupting the stability of output current, to remove these at the front end, low pass filter with 300Hz cutoff frequency is designed and placed. And also to minimize switching noise of the current into magnet and to stop abrupt fluctuations, output filter should be designed, when doing this, we design it by considering load has high value inductance. As power supply demands the stability of less than 5ppm, high precision 24bit(300nV/bit) analog digital converter is needed. As resolving power of 24bit(300nV/bit) analog digital converter is high, it is also very important to design the input stage of analog digital converter. To remove input noise, 4th order low pass filter is composed. Due to the limitation of clock, to minimize quantization error between 15bit DPWM and output of ADC having 24bit resolving power, ${\Sigma}-{\Delta}$ modulation is used and bit contracted DPWM is constituted. And before implementing, to maximize efficiency, simulink is used.

• 한국산업보건학회지
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• 제1권2호
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• pp.238-245
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• 1991

This study was analysis of ${\alpha}$-quartz in the 37 worker's lung died of coal workers' pneumoconiosis(CWP) and in the 9 normal's lung by Fourier transform infrared spectrophotometer. The results were as follows : 1. The dried lung weight percents in wet lung weight were 24.4%, 25.4%, coal workers' pneumoconiosis and normal lungs, respectively. Ash weight percents in dried lung weight were 7.7%, 5.0%, coal workers' pneumoconiosis and normal lungs, respectively. 2. The presision (as of coefficient of variation) for the ${\alpha}$-quartz determination were 2.6% in standard quartz (ranged from 9.9 to $198.0{\mu}g$) and 3.7% in ashed lung samples. 3. The characterstic ${\alpha}$-quartz absorption spectra in lungs of CWP were only shawn by LTA ashing. 4. Geometric mean of ${\alpha}$-quartz concentration in CWP lung was 173mg/100g dried lung. 5. The concentrations of ${\alpha}$-quartz in Korean CWP lungs were similar to those of foreign coal miner's lungs.

• Earthquakes and Structures
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• 제11권6호
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• pp.1143-1164
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• 2016

Time Delay of Arrival (TDOA) estimation methods based on correlation function analysis play an important role in the micro-seismic event monitoring. It makes full use of the similarity in the recorded signals that are from the same source. However, those methods are subjected to the noise effect, particularly when the global similarity of the signals is low. This paper proposes a new approach for micro-seismic monitoring based on cross wavelet transform. The cross wavelet transform is utilized to analyse the measured signals under micro-seismic events, and the cross wavelet power spectrum is used to measure the similarity of two signals in a multi-scale dimension and subsequently identify TDOA. The offset time instant associated with the maximum cross wavelet transform spectrum power is identified as TDOA, and then the location of micro-seismic event can be identified. Individual and statistical identification tests are performed with measurement data from an in-field mine. Experimental studies demonstrate that the proposed approach significantly improves the robustness and accuracy of micro-seismic source locating in mines compared to several existing methods, such as the cross-correlation, multi-correlation, STA/LTA and Kurtosis methods.

• Journal of Microbiology and Biotechnology
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• 제26권9호
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• pp.1517-1526
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• 2016

Lactic acid bacteria (LAB) isolated from fermented foods have potential as a treatment for immune-related disorders and the use of LAB has been increasing worldwide. In this study, the differential cytokine regulatory effect was examined with three isolates of lactobacilli strains; namely, Lactobacillus plantarum K55-5 isolated from dairy product, and L. sakei K101 and L. plantarum K8 previously isolated from kimchi (a Korean traditional fermented vegetable). Production of cytokines such as IL-10, IL-12, IFN-γ, and TNF-α was significantly increased in L. sakei K101- and L. plantarum K55-5-treated splenocytes as compared with controls. The oral administration of L. sakei K101 and L. plantarum K55-5 increased cytokine production in the immunosuppressed mouse splenocytes and blood. NK cell cytotoxic activity was also increased in L. sakei K101- and L. plantarum K55-5-fed mice. On the other hand, L. plantarum K8 did not affect cytokine induction in all the experiments performed in this study. The cytokine-inducing effect of L. plantarum K55-5 was significantly increased by lysates of heat-killed bacteria as compared with live, heat-killed, or supernatant of cell lysates. TNF-α production by lipoteichoic acids (LTAs) isolated from the three isolates of lactobacilli was compared, and it was found that K55-5 LTA had a highest cytokine-inducing ability, which was mediated by TLR2-mediated NF-κB and ERK activation. Taken together, our study suggests that L. plantarum K55-5 and L. sakei K101 can be used for the treatment of immunosuppressed disorders.

• 대한후두음성언어의학회지
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• 제16권1호
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• pp.10-14
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• 2005

Background and Objectives : Vowels are characterized on the basis of formant patterns. The first formant(F1) is determined by high-low placement of the tongue, and the second formant (F2) by front-back placement of the tongue. The fundamental frequency(F0) of a soprano often exceed the normal frequency of the first formant. And the vocal intensity is boosted when F0 is high and a harmonic coincides with a formant. This is called a formant tuning. Experienced singers thus learned how to tune their formants over a resonable range by lowering the tongue to maximize their vocal intensity. So, the current study aimed to identify the formant tuning in one experienced soprano by comparing the first formants of vowel [i] in three different voice production : speech, ascending scale, and vocalize. Materials and Method : All voices recordings of vowel [i] in speech, ascending scale (from F4 note to A4 note), and vocalize(:Ridente la calam") were made with digital audio tape-corder in a sound treated room. And the captured data were analyzed by the long term average(LTA) power spectrum using the FFT algorithm of the Computerized Speech Lab(CSL, Kay elementrics, Model, 4300B). Results : Although the first formant of vowel [i] in speech was 238Hz, those of ascending scale [i] were 377Hz, 405Hz, 453Hz respectively in F4(349z), G4(392Hz), A4(440Hz) note, and 722Hz, 820Hz, 918Hz respectively in F5 (698Hz), G5(784Hz), A5(880Hz) note. In vocalize, first formants of [i] were 380Hz, 398Hz, 453Hz respectively in F4, G4, A4 note, and 720Hz, 821Hz, 890Hz respectively in F5, G5, A5 note. Conclusion : These results showed that the first formant of ascending scale and vocalize sustained higher frequency than fundamental frequency in high pitch. This finding implicates that the formant tuning of vowel [i] in ascending scale was also noted in vocalize.

• 한국축산식품학회지
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• 제34권6호
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• pp.829-835
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• 2014

Inflammatory bowel disease (IBD) is caused by dysregulation of colon mucosal immunity and mucosal epithelial barrier function. Recent studies have reported that lipoteichoic acid (LTA) from Lactobacillus plantarum K8 reduces excessive production of pro-inflammatory cytokine. In this study, we investigated the preventive effects of lysate of Lb. plantarum K8 in dextran sulfate sodium (DSS)-induced colitis. Male Sprague-Dawley rats were orally pretreated with lysate of Lb. plantarum K8 (low dose or high dose) or live Lb. plantarum K8 prior to the induction of colitis using 4% DSS. Disease progression was monitored by assessment of disease activity index (DAI). Histological changes of colonic tissues were evaluated by hematoxylin and eosin (HE) staining. Tumor necrosis factor-alpha (TNF-${\alpha}$), interleukin-6 (IL-6) levels were measured using enzyme-linked immunosorbent assay (ELISA). The colon mRNA expressions of TNF-${\alpha}$, IL-6, and toll like receptor-2 (TLR-2) were examined by quantitative real-time-transcription polymerase chain reaction (qPCR). Lysate of Lb. plantarum K8 suppressed colon shortening, edema, mucosal damage, and the loss of DSS-induced crypts. The groups that received lysate of Lb. plantarum K8 exhibited significantly decreased levels of the pro-inflammatory cytokines TNF-${\alpha}$ and IL-6 in the colon. Interestingly, colonic expression of toll like receptor-2 mRNA in the high-dose lysate of Lb. plantarum K8 group increased significantly. Our study demonstrates the protective effects of oral lysate of Lb. plantarum K8 administration on DSS-induced colitis via the modulation of pro-inflammatory mediators of the mucosal immune system.

• 한국식품저장유통학회지
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• 제31권3호
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• pp.462-473
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• 2024

본 연구에서는 벨루가 렌틸 추출물(BLE)의 항염증 효능을 NO 생성 측면에서 검토하고 이와 관련된 분자 기전을 규명하고자 하였다. LPS, LTA 및 지방세포 공동배양에 의해 활성화된 RAW 264.7 대식세포에서 BLE는 독성이 없는 농도 범위에서 유의적인 NO 생성 저해 효과를 보였다. 이와 함께 BLE 처리에 따라 iNOS mRNA 및 단백질의 발현이 유의적으로 저해됨을 확인하였으며, 이는 BLE에 의한 NO 생성 억제 효과가 전사 수준에서의 iNOS 발현을 억제함으로부터 기인하는 것임을 시사한다. 추가적으로 BLE는 NO 외에도 활성화된 대식세포에서 증가하는 다양한 염증성 사이토카인 유전자(COX-2, IL-1β 및 IL-6)의 mRNA 발현 또한 유의적으로 억제하는 것으로 나타났다. BLE의 항염증 효과와 관련된 분자 기전에 관한 추가적인 연구를 수행한 결과, 항염증 관련 대표 기전인 NF-κB/MAP kinases 신호 경로와는 연관성이 없는 것으로 나타났다. 반면, BLE 처리에 따라 Nrf2 단백질 발현의 증가 및 활성화가 유도되고 HO-1의 발현이 유의적으로 증가하는 것을 확인함으로써 Nrf2가 HO-1의 발현을 증가시키기 위한 전사 인자로서 작용할 가능성이 높음을 확인하였다. 따라서, 본 연구를 통해 식용원료 유래의 잠재적인 항염증 소재로서 BLE의 활용 가능성을 확인할 수 있었으며, 향후 동물모델을 대상으로 항염증 효과를 입증하기 위한 추가적인 연구가 필요할 것으로 생각된다.

• 한국조경학회지
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• 제42권4호
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• pp.30-36
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• 2014

본 연구는 식재설계시 주요 참고자료인 조달청, (사)한국물가협회, (사)한국조경수협회(이하, 고시기관 및 단체)의 자료를 바탕으로 과거 25년 동안 고시된 철쭉류 가격 및 종수의 추이변동을 분석하였다. 고시기관 및 단체에서 고시한 철쭉류의 가격변동은 25년 동안 유사한 패턴으로 움직였다. 이는 (사)한국조경수협회에서 고시한 철쭉류 가격을 다른 고시기관 및 단체에서 참고해 가격을 책정하기 때문이었다. 조달청은 다른 고시기관 및 단체에 비해 철쭉류 고시가격을 낮게 책정했는데, 이는 정부의 물가억제 정책으로 인해 조경수목 가격 상승을 억제하기 위함으로 판단된다. 철쭉류는 다른 조경수목에 비해 생산기간이 짧아 소비자물가 변동률의 영향보다 철쭉류의 수급량의 불균형에 따라 가격이 급변하는 것으로 보인다. 고시기관 및 단체의 철쭉류 고시가격은 실거래 가격보다 높게 책정되었다. 이는 식재공사의 하도급 관행으로 인해 하자비용 충당 및 하도급 시공업체의 이익 발생을 위한 것으로 판단된다. 고시기관 및 단체에서 고시한 철쭉류는 5~8가지로 과거부터 주로 이용된 철쭉류만을 단순하게 고시하였다. 철쭉류의 재배품종명 및 분류기준이 확실하지 않아, 관행처럼 설계자는 명확한 재배품종명을 식재도면에 기재하지 않고, 시공자도 구하기 쉬운 철쭉류를 심었다. 이로 인해 새로운 철쭉류의 수요가 없어 생산농가에서는 신품종 개발 의욕이 저하되고, 과거부터 주로 이용된 철쭉류만이 생산 고시되는 악순환이 반복된 것으로 추정된다.