• 대한방사선방어학회:학술대회논문집
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• 2011.11a
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• pp.212-213
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• 2011

The biological effects of low dose ionizing radiation (LDIR) remain insufficiently understood. We examined for the scientific evidence to show the biological effects of LDIR using radiation-sensitive immune cells. We found that Ikaros protein was responsed to low dose-dependent effects of gamma radiation in IM-9 B lymphocytes. Ikaros encodes zinc finger transcription factors that is important regulators of a hematopoietic stem cells (HSCs) progression to the B lymphoid lineage development, differentiation and proliferation. In this study, we observed that cell proliferation was enhanced from 10% to 20% by LDIR (0.05 Gy) in IM-9 B lymphocytes. The Ikaros protein was phosphorylated in its serine/threonine (S/T) region and decreased its DNA binding activity in the cells exposed to LDIR. We found that Ikaros phosphorylation was up-regulated by CK2/AKT pathway and the residues of ser-304 and ser-306 in Ikaros was phosphorylated by LDIR. We also observed that Ikaros protein was localized from the nucleus to the cytoplasm after LDIR and bound with Autotaxin (ENPP2, ATX) protein, stimulating proliferation, migration and survival of immune cells. In addition, we found that the lysoPLD activity of ATX was dependent on Ikaros-ATX binding activity. These results indicate that the Ikaros is an important regulator of immune activation. Therefore, we suggest that low dose ionizing radiation can be considered as a beneficial effects, stimulating the activation of immune cells.

• BMB Reports
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• v.39 no.5
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• pp.626-635
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• 2006

Lysophosphatidic acid acyltransferase (LPAAT) is an intrinsic membrane protein that catalyzes the synthesis of phosphatidic acid (PA) from lysophosphatidic acid (LPA). It is well known that LPAAT is involved in lipid biosynthesis, while its role in tumour progression has been of emerging interest in the last few years. To date, seven members of the LPAAT gene family have been found in human. Here we report a novel LPAAT member, designated as LPAAT-theta, which was 2728 base pairs in length and contained an open reading frame (ORF) encoding 434 amino acids. The LPAAT-theta gene consisted of 12 exons and 11 introns, and mapped to chromosome 4q21.23. LPAAT-theta was ubiquitously expressed in 18 human tissues by RT-PCR analysis. Subcellular localization of LPAAT-theta-EGFP fusion protein revealed that LPAAT-theta was distributed primarily in the endoplasmic reticulum (ER) of COS-7 cells. Furthermore, we found that the overexpression of LPAAT-theta can induce mTOR-dependent p70S6K phosphorylation on Thr389 and 4EBP1 phosphorylation on Ser65 in HEK293T cells.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.4
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• pp.1443-1448
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• 2015

To investigate the significance of FOXO3a and Ki67 in human lung adenocarcinomas. Envision immunohistochemical staining and Western blotting were used to examine the protein expression of FOXO3a in 127 cases of human lung adenocarcinoma specimens. The positive rate in lung adenocarcinoma (55.9%) was lower than that in normal tissues (80%). We found that the expression of FOXO3a was closely related with the degree of differentiation, TNM staging, lymph node metastasis and survival. In addition, significant differences in the different pathological types of lung adenocarcinoma cases (P<0.01). The FOXO3a positive rate of the acini as the main type (APA) (86.7%) and the lepidic as the main type (LPA) (82.4%) was higher than the solid as the main type (SPA) (50.0%), the papilla as the main type (PPA) (42.9%) and the micropapilla as the main type (MPA) (9.4%). Moreover, the expression of FOXO3a was negatively related with Ki67 expression. Our results suggested that the expression of FOXO3a is closely correlated with the aggressiveness of lung adenocarcinoma. It was indicated that disregulation of FOXO3a might play key roles in the occurrence and development of lung a denocarcinoma and joint detection of the two markers might play an important role in diagnosing tumors.

• Membrane Journal
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• v.11 no.3
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• pp.124-132
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• 2001

Silica membranes were prepared on a porous ${\alpha}$-alumina tube with pore size of 150nm by sol-gel and chemical vapor deposition(CVD) method for hydrogen separation at high temperatures. Silica and ${\gamma}$-lumina membranes formed by the sol-gel method possessed a large amount of mesopores of a Knudsen diffusion regime. In order to improve the $H_2$ selectivity, silica was deposited in the sol-gel derived silica/${\gamma}$-alumina layer by thermal decomposition of tetraethyl orthosilicate(TEOS) at $600^{\circ}C$. The CVD with forced cross flow through the porous wall of the support was very effective in plugging mesopores that were left unplugged in the membranes. The CVD modified silica/alumina composite membrane completely rejected nitrogen permeation and thus showed a high $H_2$ selectivity by molecular sieve effect. the permeation of hydrogen was explained by activated diffusion and the activation energy was 9.52kJ/mol.

• Proceedings of the KOSOMBE Conference
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• v.1996 no.05
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• pp.203-206
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• 1996

To understand the local fluid dynamics for different desists of Fontan operation, five models were made out of Pyrex glass to facilitate in-vitro study. Model I, II and III have same position of the center of the anastomosis of the IVC( inferior vena cava) with that of the SVC(superior vena cava), but Model IV and V have 10 mm offset between them. Also the anastomotic junction angles are different(Model I and $IV:90^{\circ}$, Model II and $V:70^{\circ}$, Model $III:45^{\circ}$). These models were then connected to a flow loop for flow visualization study. In Model I any dominant vortex was not seen in the central region of the juntion, but a large unstable vortex was created in the Model II and III. In Model IV and V a significant stagnation region was created in the middle of the offset region. It also showed that the flow direction from the IVC and SVC to the LPA(left pulmonary artery) and RPA(right pulmonary artery) highly depends on the offset of the junction rather than the anastomotic junction angle.

• Journal of Radiation Industry
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• v.10 no.1
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• pp.7-12
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• 2016

Ikaros, a transcription factor containing zinc-finger motif, has known as a critical regulator of hematopoiesis in immune system. Ikaros protein modulates the transcription of target genes via binding to the regulatory elements of the genes promoters. However the regulatory function of Ikaros in other organelle except nuclear remains to be determined. This study explored radiation-induced modulatory function of Ikaros in cytoplasm. The results showed that Ikaros protein lost its DNA binding ability after LDIR (low-dose ionizing radiation) exposure. Cell fractionation and Western blot analysis showed that Ikaros protein was translocated into cytoplasm from nuclear by LDIR. This was confirmed by immunofluorescence assay. We identified Autotaxin as a novel protein which potentially interacts with Ikaros through in vitro protein-binding screening. Co-immunoprecipitation assay revealed that Ikaros and Autotaxin are able to bind each other. Autotaxin is a crucial enzyme generating lysophosphatidic acid (LPA), a phospholipid mediator, which has potential regulatory effects on immune cell growth and motility. Our results indicate that LDIR potentially regulates immune system via protein-protein interaction of Ikaros and Autotaxin.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.31 no.3
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• pp.237-254
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• 2021

Objectives: This study aims to classify the latent profiles in the exposure to hazard factors of salaried employees and test the determinants. Methods: Latent profile analysis(LPA) was conducted using data from the fifth Korean Working Conditions Survey(KWCS). 30,050 of salaried employees were the subjects of this study. After classifying the employees, multinomial logistic regression was used to test the determinants. Results: Salaried employees were classified with three latent profiles based on the exposure to the hazard factors. Employees included in class 1(32.8%) tend to experience low level of physical hazard factors, moderate level of psychological hazard factors, and high level of office work hazard factors. Employees included in class 2(61.8%) tend to be exposed to the moderate to high level of physical hazard factors, moderate to low level of psychological hazard factors, and low level of office work hazard factors. Employees included in class 3(5.4%) tend to experience high level of psychological and physical hazard factors, and moderate level of office work hazard factors. After classification, the demographic, health-, and employment-related variables were tested. Conclusions: This study clarified the features of each class, and proved that employees in class 3 are quite hazardous in that they are exposed to physical and psychological hazard factors much more frequently than other employees. Thus, this study can be used in predicting the high-risk employees and taking preemptive measures for preventing industrial accidents.

• The Journal of the Korea Contents Association
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• v.21 no.7
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• pp.533-545
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• 2021

This study aimed to examine the differences in gender equality consciousness between latent profiles based on corporate workers' organizational culture types. To achieve the study's purpose, descriptive statistics, correlation analysis, and latent profile analysis (LPA) were conducted using 253 Korean corporate workers' data. As a result of the analysis, the latent profiles of organizational culture recognized by corporate workers were classified into 5 profiles. We named each profile as high compete-low create culture, high compete-low collaborate culture, collaborate culture, high create culture, and high control culture based on each profile's organizational culture perception. The ANOVA test found statistically significant differences between the groups in the gender equality consciousness. This study is meaningful in that it reveals the relationship between the organization culture perception and the gender equality consciousness and suggested implications for the organizational-level intervention to improve the gender equality consciousness. It will provide basic data for your research.

• Journal of Family Relations
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• v.23 no.1
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• pp.25-51
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• 2018

Objectives: The aim of this study is to identify patterns of gender role attitude among older men and women and to investigate the effects of the identified patterns on marital quality for each gender. Method: The Third National Survey of Korean Families was utilized and 981 men and 752 women aged 60 and above, who have been married for over 20 years and have at least one child, were analyzed. Using Mplus 7.3, a latent profile analysis (LPA) identified latent profiles of gender role attitude for each gender. Then, using SPSS 23.0, multiple regression analysis analyzed the effects of the identified patterns on both marital satisfaction and marital conflict. Results: The latent profile analysis identified two patterns of traditional and partially egalitarian gender role attitudes each for the older men and women. Also, means on items relevant to the male breadwinner model and the male-oriented family culture were relatively low for both men and women regardless of the patterns. For the older men, the effects of the gender role attitude patterns on marital satisfaction and marital conflict were both significant; those individuals with the partially egalitarian gender role attitude pattern reported higher levels of marital satisfaction and lower levels of marital conflict than those individuals with the traditional gender role attitude pattern. However, the effects of the gender role attitude patterns on marital satisfaction and marital conflict were both not significant for the older women. Conclusions: The findings from this study suggest that political interventions are required to promote an egalitarian family culture for older adults. Moreover, its findings may be useful in the field of marriage and family therapy to develop educational programs that seek to improve marital quality in later life.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.16 no.1
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• pp.37-53
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• 2021

The purpose of this study is to analyze the types of latent profiles depending on the job satisfaction of youth entrepreneurs in South Korea and examine the influence of related variables. Latent profile analysis (LPA) was conducted using data from the 12th year of the Youth Panel Study. Results of the study revealed that there were five latent profiles based on job satisfaction among young entrepreneurs: lowest job satisfaction, mid-low job satisfaction, middle job satisfaction, mid-high job satisfaction, and highest job satisfaction. By applying multinomial logistic analysis, the influencing factors for each latent profiles have been derived. Gender, marital status, subjective health, self-esteem, entrepreneurial motivation, difficulty in start-up, person-job fit, intrinsic or extrinsic job values, business scale, income, work hour, education or training, and labor insurance were significant predictors of youth entrepreneurs' job satisfaction. Based on these results, the article discusses practical implications that can enhance the job satisfaction of young entrepreneurs.