• International Journal of Aerospace System Engineering
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• v.3 no.1
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• pp.30-38
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• 2016

The The turbulent mixing characteristics of Kerosene-LOx in a coaxial swirl injector 100 bar have been numerically investigated. Turbulent model is based on large eddy simulation with real-fluid transport and thermodynamics. The effects of equation of state (EOS), chamber pressure are evaluated in a point of the mixing efficiency and pressure fluctuations. The dominant frequency is same as the hairpin vortex shedding frequency generated by film wave at the LOx post.

• Bulletin of the Korean Space Science Society
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• 2004.10b
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• pp.262-265
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• 2004

As a cryogen, LOx is a light blue, odorless, transparent liquid. Also it is not shock sensitive and does not decompose. However, it is a strong oxidizer and will vigorously support combustion. Therefore all harmful contaminants (such as grease, oil, fingerprint and organic materials) that could cause malfunctions, fires, or explosions in a oxygen environments must be completely removed prior to the introduction of oxygen. Especially, grease ingredient located inside of the LOx supply line, pipe and PHS (Pneumo-Hydraulic System) part can make drastic chemical reaction with oxygen. Therefore, to protect rapid reaction such as explosion, grease ingredient must be surely eliminated by a regular and irregular degreasing. Study on the availability, effectiveness and selection of degreasing detergents and method is described in this paper, and it will be useful for the construction and management of IPP test facility.

• BMB Reports
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• v.29 no.6
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• pp.564-568
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• 1996

Bursts of ethylene production occurred in twice at an early exponential (EEP) and prestationary (PSP) phases, respectively, during growth of callus tissue isolated from the root of soybean seedlings. The second burst of ethylene production at PSP was smaller in magnitude than the earlier one at EEP, but was followed by increases in both guaiacol peroxidase (GuPOX) and ascorbate peroxidase (AsPOX). The increase in AsPOX activity was also preceded by an increase in lipoxygenase (LOX) activity. Treatment of the tissue with the ethylene antagonist 2,5-norbonadiene (NBD) resulted in substantial reduction in LOX and AsPOX activities during this period. GuPOX activity was reduced only slightly, if any, by NBD. Role of ethylene in the sequential induction of LOX and AsPOX in senescing callus tissue is discussed.

• Journal of the Korean Society of Propulsion Engineers
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• v.2 no.3
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• pp.90-98
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• 1998

The vaporization process of liquid oxygen(LOX) at high pressure environment is numerically investigated. The present vaporization model can account for the high-pressure effects such as ambient gas solubility, real gas behavior and variable properties. The predicted phase-equilibrium compositions for $N_2$/$H_2$ and $O_2$/He system are well agreed with experimental data. The LOX vaporization characteristics is parametrically studied for wide range of the operating conditions encountered in the high-pressure combustion process of liquid rocket engine.

• Bulletin of the Korean Space Science Society
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• 2003.10a
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• pp.70-70
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• 2003

액체 로켓 엔진에 있어 극저온 추진제인 액체 산소를 사용하는 경우에는 He을 가압제로 사용하는 것이 가장 바람직하지만, 기체인 헬륨은 발사 대기시, 선가압시, 비행중에 액체산소에 서서히 녹게 된다. 일정량 이상의 He이 용해되어 있는 LOX가 엔진에 공급되는 경우에는 터보펌프의 이상 작동 또는 연소 불안정을 야기하게 되므로, 추진기관이 작동하는 동안에 용해되어 있던 He이 액체 산소에서 분해되어 가스로 발생되는지 여부를 판단하고, 이는 엔진의 연소 시험을 통해서 검증되어야 한다. 본 연구에서는 가상의 작동 상태에 대해 최대로 용해될 수 있는 러e의 양을 계산하고, 현재 사용되는 발사체의 경우와 비교를 하여 추진시스템 운용 조건을 적절히 조절하는 방안을 제시하였다.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.3
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• pp.369-377
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• 2013

In order to increase the utilization of sweet potato leaves and stalks as much as roots, it is necessary to study their beneficial potential. In this study, the antioxidant, antiallergic and anti-inflammatory effects of sweet potato leaves and stalks were evaluated by measuring total polyphenol and flavonoid contents, DPPH radical scavenging effects, the reducing power and inhibition effects on xanthine oxidase (XO), 5-lipoxygenase (LOX), and cyclo-oxygenase (COX)-2 activities. Blanched sweet potato leaves (SL), raw whole purple stalks (ST) and peeled stalks (PST) were freeze-dried and extracted with 95% ethanol. Total polyphenol content was highest in SL (11.03 mg/g), followed by ST (0.87 mg/g), and PST (0.37 mg/g). Total flavonoid content was highest for SL (9.01 mg/g), followed by ST (0.50 mg/g) and PST (0.25 mg/g). The $IC_{50}$ for DPPH radical scavenging effects was highest for SL ($43.6{\mu}g/mL$), followed by ST ($308.4{\mu}g/mL$) and PST ($1,631.3{\mu}g/mL$). The reducing power was highest for SL ($59.72{\mu}g$ ascorbic acid eq./mL), followed by ST ($12.56{\mu}g$ ascorbic acid eq./mL) and PST ($2.18{\mu}g$ ascorbic acid eq./mL) with $1,000{\mu}g/mL$ of ethanol extract. The inhibition rate on XO activity was highest for SL (13.06%), followed by ST (5.05%) and PST (0.0%) at $250{\mu}g/mL$ extract treatment. The inhibition rate on COX-2 activity was highest for SL (55.34%), followed by ST (2.18%) and PST (0.0%) at $250{\mu}g/mL$ extract treatment. The inhibition rate on 5-LOX activity was highest for SL (91.16%), followed by ST (33.38%) and PST (14.93%) at $50{\mu}g/mL$ treatment. Taken together, sweet potato leaves showed high antioxidative, anti-allergic and anti-inflammatory activities, especially with very strong inhibition effects on 5-LOX activity. These beneficial effects of sweet potato leaves might be mainly caused by the high content of polyphenols and flavonoids.

• Mycobiology
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• v.42 no.2
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• pp.185-188
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• 2014

We investigated a total of 335 samples of Korean native mushroom extracts as part of our lipoxygenase (LOX) inhibitor screening program. Among the mushroom-methanolic extracts we investigated, 35 exhibited an inhibitory activity greater than 30% against LOX at a concentration of 100 ${\mu}g/mL$. Especially, Collybia maculata, Tylopilus neofelleus, Strobilomyces confusus, Phellinus gilvus, P. linteus, P. baumii, and Inonotus mikadoi exhibited relatively potent LOX inhibitory activities of 73.3%, 51.6%, 52.4%, 66.7%, 59.5%, 100.0%, and 85.2%, respectively. Bioassay-guided fractionation led to the isolation of inoscavin A from the methanolic extract of P. baumii, which showed the most potent activity and was identified by spectroscopic methods. Specifically, inoscavin A exhibited potent LOX inhibitory activity with an $IC_{50}$ value of $6.8{\mu}M$.

• BMB Reports
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• v.34 no.4
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• pp.322-328
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• 2001

Excision and amplification of pre-determined, large genomic segments (taken directly from the genome of a natural host, which provides an alternative to conventional cloning in foreign vectors and hosts) was explored in human cells. In this approach, we devised a procedure for excising a large segment of human genomic DNA, the iNOS gene, by using the Cre/loxP system of bacteriophage P1 and amplifying the excised circles with the EBNA-1/oriP system of the Epstein-Barr virus. Two loxP sequences, each of which serves as a recognition site for recombinase Cre, were integrated unidirectionally into the 5'-UTR and 3'-UTR regions of the iNOS gene, together with an oriP sequence for conditional replication. The traps-acting genes cre and EBNA-1, which were under the control of a tetracycline responsive $P_{hcmv^*-1}$ promoter, were also inserted into the 5'-UTR and 3'-UTR regions of the iNOS gene, respectively, by homologous recombination. The strain carrying the inserted elements was stably maintained until the excision and amplification functions were triggered by the induction of cre and EBNA-1. Upon induction by doxycycline, Cre excised the iNOS gene that was flanked by two ZoxP sites and circularized it. The circularized iNOS gene was then amplified by the EBNA-1/oriP-system. With this procedure, approximately a 45.8-kb iNOS genomic fragment of human chromosome 17 was excised and successfully amplified in human cells. Our procedure can be used effectively for the sequencing of unclonable genes, the functional analysis of unknown genes, and gene therapy.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2008.11a
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• pp.137-142
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• 2008

Ignition characteristics of combustion chamber with LOx lead cyclogram for liquid rocket engine were described. The combustion chamber has chamber pressure of 60 bar, propellant mass flow rate of 89 kg/s, and nozzle expansion of 12. Cold flow test to determine the filling time of propellant for cyclogram with LOx lead supply, ignition test to check the ability to ignite starting fuel from the ignitor, low pressure combustion test to check the propagation of flame into main fuel-oxidizer mixture from starting fuel and the main combustion stage, and design point combustion test to check the combustion performance were performed. Ignition and combustion tests with LOx lead supply were successfully performed and the stable cyclogram of start sequence for combustion chamber was developed.

• Toxicological Research
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• v.35 no.1
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• pp.83-91
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• 2019

Nanoparticles (NPs) have been recognized as both useful tools and potentially toxic materials in various industrial and medicinal fields. Previously, we found that zinc oxide (ZnO) NPs that are neurotoxic to human dopaminergic neuroblastoma SH-SY5Y cells are mediated by lipoxygenase (LOX), not cyclooxygenase-2 (COX-2). Here, we examined whether human bone marrow-derived mesenchymal stem cells (MSCs), which are different from neuroblastoma cells, might exhibit COX-2- and/or LOX-dependent cytotoxicity of ZnO NPs. Additionally, changes in annexin V expression, caspase-3/7 activity, and mitochondrial membrane potential (MMP) induced by ZnO NPs and ZnO were compared at 12 hr and 24 hr after exposure using flow cytometry. Cytotoxicity was measured based on lactate dehydrogenase activity and confirmed by trypan blue staining. Rescue studies were executed using zinc or iron chelators. ZnO NPs and ZnO showed similar dose-dependent and significant cytotoxic effects at concentrations ${\geq}15{\mu}g/mL$, in accordance with annexin V expression, caspase-3/7 activity, and MMP results. Human MSCs exhibited both COX-2 and LOX-mediated cytotoxicity after exposure to ZnO NPs, which was different from human neuroblastoma cells. Zinc and iron chelators significantly attenuated ZnO NPs-induced toxicity. Conclusively, these results suggest that ZnO NPs exhibit both COX-2- and LOX-mediated apoptosis by the participation of mitochondrial dysfunction in human MSC cultures.