• Journal of Microbiology and Biotechnology
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• v.27 no.5
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• pp.965-974
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• 2017

The single-chain variable fragment (scFv) against lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is a promising molecule for its potential use in the diagnosis and immunotherapy of atherosclerosis. Producing this scFv in several milligram amounts could be the starting point for further engineering and application of the scFv. In this study, the abundant expression of the anti-LOX-1 scFv was attempted using Escherichia coli (E. coli) and Brevibacillus choshinensis (B. choshinensis). The scFv had limited soluble yield in E. coli, but it was efficiently secreted by B. choshinensis. The optimized fermentation was determined using the Plackett-Burman screening design and response surface methodology, under which the yield reached up to 1.5 g/l in a 5-L fermentor. Moreover, the properties of the scFvs obtained from the two expression systems were different. The antigen affinity, transition temperature, and particle diameter size were 1.01E-07 M, $55.2{\pm}0.3^{\circ}C$, and 9.388 nm for the scFv expressed by B. choshinensis, and 4.53E-07 M, $52.5{\pm}0.3^{\circ}C$, and 13.54 nm for the scFv expressed by E. coli. This study established an efficient scale-up production methodology for the anti-LOX-1 scFv, which will boost its use in LOX-1-based therapy.

• Journal of the Korean Society of Propulsion Engineers
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• v.9 no.3
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• pp.25-37
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• 2005

For pump-fed rocket propulsion system, the temperature of LOX to be supplied to turbopump inlet should be satisfied with pump inlet temperature requirement during all operating stages, as excessive temperatures can result in cavitation due to reduction in NPSH, thus either damaging the pump or adversely affecting pump performance rise. So exact estimation of LOX temperature rise is absolutely needed for developing reliable propulsion system. This paper presents systematic analysis scheme for estimating inner process of cryogenic propellant tank which is needed for LOX temperature rise. And this paper presents LOX temperature rise and thermal stratification for all rocket operating stages including cooling, filling, waiting, pre-pressurization and firing, with the application of buoyancy driven boundary layer theory.

• Aerospace Engineering and Technology
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• v.6 no.2
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• pp.205-210
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• 2007

A LOX pump rotordynamic design was performed for a 75 ton thrust liquid rocket engine. Axial positions of an inducer, an impeller and bearings on a shaft are decided on the basis of the experience achieved by previously developed turbopump which has the similar layout. The result of pump hydraulic design was reflected in the present study to decide axial length of the inducer and impeller. A distance from the rear bearing to the impeller was considered as a design parameter for load distribution of the bearings. Asynchronous eigenvalue analysis was performed as a function of rotating speeds and bearing stiffness to investigate critical speed of the LOX pump. From the numerical analysis, it is found that the LOX pump with the proper bearing loads safely operates as a sub-critical rotor of which critical speed is high enough compared to the operating speed 11,000 rpm.

• Biomolecules & Therapeutics
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• v.20 no.1
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• pp.113-117
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• 2012

The fruits of Foeniculum vulgare (Foeniculi Fructus) have been widely used in Chinese medicine as an antiemetic, ameliorating stomach ailments and as an analgesic. In order to establish its potential for antiallergic use, inhibitory actions of the fruit on 5-lipoxgenase (5-LOX) and ${\beta}$-hexosaminidase release were evaluated. The 70% ethanol extract of this plant material (FR) considerably inhibited 5-LOX-catalyzed leukotriene production from A23187-induced rat basophilic leukemia (RBL)-1 cells. The $IC_{50}$ was $3.2{\mu}g/ml$. From this extract, 12 major compounds including sabinene, fenchone, ${\gamma}$-terpinene, ${\alpha}$-pinene, limonene, p-anisylacetone, panisylaldehyde, estragole (4-allylanisole), trans-anethole, scopoletin, bergapten and umbelliferone were isolated. And it was found that several terpene derivatives including ${\gamma}$-terpinene and fenchone as well as phenylpropanoid, trans-anethole, showed considerable inhibitory action of 5-LOX. In particular, the $IC_{50}$ of trans-anethole was $51.6{\mu}M$. In contrast, FR and the isolated compounds did not show considerable inhibitory activity on the degranulation reaction of ${\beta}$-hexosaminidase release from antigen-treated RBL-2H3 cells. Against arachidonic acid-induced ear edema in mice, FR and trans-anethole showed significant inhibition by oral administration at doses of 100-400 mg/kg. In conclusion, FR and several major constituents are 5-LOX inhibitors and they may have potential for treating 5-LOX-related disorders.

• Korean Chemical Engineering Research
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• v.59 no.3
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• pp.373-378
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• 2021

The study aimed to develop a high-power enzymatic electrode for a wearable fuel cell that generates electricity utilizing lactate present in a sweat as fuel. Anode was fabricated by immobilizing lactate oxidase (LOx) on flexible carbon paper. As the lactate concentration in the electrolyte solution increased, the amount of current generated by catalysis of lactate oxidase increased. The immobilized LOx generated 1.5-times greater oxidation current density in the presence of gold nanoparticles than carbon paper only. Bilirubin oxidase (BOD)-immobilized cathode generated a larger amount of reduction current in the electrolyte saturated with oxygen than purged with nitrogen. A fuel cell composed of two electrodes was fabricated and cell voltage was measured under different discharge current. At the discharge current density of 66.7 ㎂/cm², the cell voltage was 0.5±0.0 V leading to maximum cell power density of 33.8±2.5 ㎼/cm².

• Proceedings of the KSME Conference
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• 2001.06e
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• pp.276-281
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• 2001

The KSR-III Main Propulsion System configuration of the liquid oxygen (LOX) feed line is analyzed. This feed line includes a tighter radius and cavitation venturi for flow mass flow-rate passive control. There were concerns that these configurations might generate a great flow distortion at the engine interface. Also both the pressure drop at the feed line and any presence of separation area are a great concern according to the propellant flow. To resolve these issues, a computational fluid dynamic analysis was conducted to determine the flow field in the LOX feed lines.

• Journal of the Korean Society of Propulsion Engineers
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• v.8 no.3
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• pp.68-74
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• 2004

Effect of fluctuation imposed on the inflow to LOX manifold of liquid rocket has been analyzed numerically. Time-varying fluctuation was idealized by the sinusoidal signal and three different representative frequencies were considered. It was found that all the frequencies tested produced the consistent flow reactions in the manifold in that the place close to the region of infection showed oscillatory flow rate through injector orifices in phase with the inflow fluctuation whereas the other side exhibits characteristics which are out of phase.

• 유체기계공업학회:학술대회논문집
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• 2006.08a
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• pp.523-526
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• 2006

A series of hydraulic and cavitation tests are performed in water environment in order to verify the hydraulic and cavitation performance of three types of LOX pumps. All the performances of the pumps are found to be satisfied with each design requirement. In the hydraulic tests, the head and efficiency are increased as the gap between floating ring seals and the impeller shoulder is decreased. In the cavitation tests, some pumps show decrease in the cavitation performance as the flowrate of the pump is decreased.

• Journal of the Korean Society for Aviation and Aeronautics
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• v.14 no.4
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• pp.17-23
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• 2006

This paper describes the preliminary design procedure for the hybrid propulsion system. For a given mission defined by velocity increment, the design of a polyethylene/LOX hybrid rocket was implemented. In addition, Seven-cluster multi-port fuel-grain was considered. After determining the system size including the combustion chamber, the performance parameters such as specific impulse, thrust, characteristic velocity, and thrust coefficient can be predicted by using empirical regression rate correlation, though most of preliminary design code assume constant regression rate. The results of the performance prediction indicated that besides the widely used HTPB/LOX, polyethylene/LOX hybrid motor can be a viable alternative to the more widely used SRMs.

• Aerospace Engineering and Technology
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• v.10 no.1
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• pp.122-128
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• 2011

A series of water tests of a Lox pump for liquid rocket engines are performed at a room temperature. According to the hydraulic test results, the head coefficient is slightly decreased when the rotational speed of the pump is increased. However the efficiency is high at the high-speed test. Also, according to the cavitation test results, the cavitation performance of the pump is found to be decreased when the flow ratio is less than 92%. Furthermore, the vibration characteristic is analyzed using measured high frequency data.