• 제목/요약/키워드: LOV

검색결과 27건 처리시간 0.018초

A New-Generation Fluorescent-Based Metal Sensor - iLOV Protein

  • Ravikumar, Yuvaraj;Nadarajan, Saravanan Prabhu;Lee, Chong-Soon;Rhee, Jin-Kyu;Yun, Hyungdon
    • Journal of Microbiology and Biotechnology
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    • 제25권4호
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    • pp.503-510
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    • 2015
  • The iLOV protein belongs to a family of blue-light photoreceptor proteins containing a light-oxygen-voltage sensing domain with a noncovalently bound flavin mononucleotide (FMN) as its chromophore. Owing to advantages such as its small size, oxygen-independent nature, and pH stability, iLOV is an ideal candidate over other reporter fluorescent proteins such as GFP and DsRed. Here, for the first time, we describe the feasibility of applying LOV domain-based fluorescent iLOV as a metal sensor by measuring the fluorescence quenching of a protein with respect to the concentration of metal ions. In the present study, we demonstrated the inherent copper sensing property of the iLOV protein and identified the possible amino acids responsible for metal binding. The fluorescence quenching upon exposure to Cu2+ was highly sensitive and exhibited reversibility upon the addition of the metal chelator EDTA. The copper binding constant was found to be 4.72 ± 0.84 µM. In addition, Cu2+-bound iLOV showed high fluorescence quenching at near physiological pH. Further computational analysis yielded a better insight into understanding the possible amino acids responsible for Cu2+ binding with the iLOV protein.

일부 폐경기여성의 채식군과 일반식군의 영양섭취상태, 골대사 및 만성 퇴행성 질환의 위험인자에 관한 비교연구 (Comparison of Nutrient Intakes, Bone Metabolism and Risk Factors of Chronic Diseases between Postmenopausal Lacto-Ovo-Vegetarian and Omnivores in Kyunggi-Do)

  • 최선혜;승정자;김미현;이숙연;송숙자
    • 대한지역사회영양학회지
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    • 제4권3호
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    • pp.412-420
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    • 1999
  • The amount and type of dietary protein that effects affect bone mineral loss after menopause and different dietary patterns may influence anthropometric measurements, biochemical values, and bone metabolism. Objective : Our objective was to investigate bone metabolism and chronic disease factors in two different dietary patterns. Design: The sample consisted of 40 lacto-ove-vegetarians(LOV) and 49 omnivores aged from 47 to 85 years in good condition without any medical problems. Results : The average energy intake of LOV was 1414.0kcal and omnivores, 1403.2kcal. The difference between means was not statistically significant. The differences of means of body weight and BMI between the two groups were not significant but the systolic blood pressure of LOV group was significantly lower than omniovores(p<0.05). The values of serum cholesterol(p<0.05) and glucose(p<0.05) of LOV were signifcantly lower than that of omnivores. The serum albumin level(p<0.01) of LOV was lower than it'scounterpart but in a normal range. The intake of crude fiber(p<0.05) of LOV group was significantly higher. The urinary pH of LOV was 7.4 and that of omnivores was 6.3(p<0.001). Plant calcium(p>0.05) and plant iron(p<0.001) had positive correlations with the uninary pH but animal protein(p>0.05) and animal iron(p>0.05) had negative correlations with the urinary pH. The level of urinary biochemical marker of bone resorption, deoxypyridinoline(DPD) of LOV was significantly lower than the other group(p>0.05). The T-scores of bone mineral density for the two sites in the two groups were not significantly different. Conclusions : The DPD level and urinary pH value of LOV were more desirable for the bone metabolism that the omnivores. The diet of LOV had lower risk factors of chronic diseases than the other group.

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LOV를 이용한 가치(價値)시스템 세분화(細分化) (The Segmentation of Value System Used by LOV)

  • 심종섭
    • 산학경영연구
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    • 제14권
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    • pp.129-148
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    • 2001
  • 이 논문은 소비자의 심리묘사적변수(psychographic variables)들로 구성된 LOV(list of values)를 조사하고, 이 LOV를 이용한 소비자들의 가치체계(value system)를 세분화하는 것을 목적으로 한다. 접근방법으로서는 Rokeach의 RVS, Kahle의 LOV, 그리고 Razzaque의 Asian Values를 참고하고, 한국적 가치를 추가한 모형을 개발하여, 한국 소비자들의 가치세분화를 진행하였다. 연구결과 LOV를 이용한 우리나라 소비자들의 가치체계(value system)는 (1)영향력에 관한 가치, (2)배금성에 관한 가치, (3)최신성에 관한 가치, (4)모험성에 관한 가치, (5)구원에 관한 가치, (6)사회성에 관한 가치, (7)호기심에 관한 가치, (8)영적 성향에 관한 가치, (9)보수성과 관련된 가치의 순서로 구분이 되었다. 이는 우리나라 소비자들의 가치중심의 시장세분화에 참고자료가 될 것으로 믿는다. 이상의 한국적 가치영역과 인구통계적 변수와의 관계를 분석해 본 결과 성별, 결혼관계, 학력수준, 직업, 가족월총수입, 가족구성형태, 연령 등 전반적 인구통계적 변수와 긴밀한 관계가 있는 것으로 나타났다.

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Erdös-Faber-Lovász 추측 증명 알고리즘 (Proof Algorithm of Erdös-Faber-Lovász Conjecture)

  • 이상운
    • 한국인터넷방송통신학회논문지
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    • 제15권1호
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    • pp.269-276
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    • 2015
  • 본 논문은 지금까지 미해결 문제로 알려진 정점 색칠 문제에 대한 Erd$\ddot{o}$s-Faber-Lov$\acute{a}$sz 추측을 증명하였다. Erd$\ddot{o}$s-Faber-Lov$\acute{a}$sz 추측은 "k개의 $K_k$-클릭 합 교차 그래프는 k개의 색으로 칠할 수 있다". 즉, x(G)=k이다". Erd$\ddot{o}$s-Faber-Lov$\acute{a}$sz 추측을 증명하기 위해 본 논문은 교차되는 정점수와 한 정점에서 교차되는 클릭수를 구하여 모두 짝수이면 그래프의 최소 차수 ${\delta}(G)$ 정점을 최대독립집합 (minimum Independent set, MIS)에 포함시키는 방법을 적용하고, 둘 중 어느 하나가 홀수이면 최대 차수 ${\Delta}(G)$ 정점을 MIS에 포함시키는 방법을 적용하였다. 알고리즘 수행결과 얻은 MIS 개수가 x(G)=k이다. $3{\leq}k{\leq}8$$K_k$-클릭 합 교차 그래프에 대해 실험한 결과 모든 그래프에서 x(G)=k를 얻는데 성공하였다. 결국, "k개의 $K_k$-클릭 합 교차 그래프는 k개의 색으로 칠할 수 있다".는 Erd$\ddot{o}$s-Faber-Lov$\acute{a}$sz 추측은 성립함을 증명하였다.

ESTABLISHMENT OF A MAINTENANCE PROGRAM TO PREVENT LOSS OF OFFSITE POWER IN NUCLEAR POWER PLANTS

  • Lee, Eun-Chan;Na, Jang-Hwan
    • Nuclear Engineering and Technology
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    • 제45권6호
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    • pp.791-794
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    • 2013
  • Since the Fukushima accident in 2011, the importance of the electrical systems in nuclear power plants (NPPs) has been emphasized. The result has been that NPP regulators are enhancing their monitoring of loss of offsite power (LOOP) events. Korea Hydro & Nuclear Power Co. (KHNP) is reviewing the status and issues related to LOOPs, and is attempting to establish specific countermeasures to prevent LOOPs, because they can have severe consequences in the complicated maintenance schedule during an outage. A starting point for preventing LOOPs is the control of the loss of voltage (LOV)-initiating components. In order to reflect this in the risk assessment program, an LOV monitor is being developed for use during plant outages.

Deploying Linked Open Vocabulary (LOV) to Enhance Library Linked Data

  • Oh, Sam Gyun;Yi, Myongho;Jang, Wonghong
    • Journal of Information Science Theory and Practice
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    • 제3권2호
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    • pp.6-15
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    • 2015
  • Since the advent of Linked Data (LD) as a method for building webs of data, there have been many attempts to apply and implement LD in various settings. Efforts have been made to convert bibliographic data in libraries into Linked Data, thereby generating Library Linked Data (LLD). However, when memory institutions have tried to link their data with external sources based on principles suggested by Tim Berners-Lee, identifying appropriate vocabularies for use in describing their bibliographic data has proved challenging. The objective of this paper is to discuss the potential role of Linked Open Vocabularies (LOV) in providing better access to various open datasets and facilitating effective linking. The paper will also examine the ways in which memory institutions can utilize LOV to enhance the quality of LLD and LLD-based ontology design.

대학생소비자의 라이프스타일 유형별 특성, 인터넷 패션쇼핑몰 이미지 및 구매행동에 관한 연구 (Study on the Traits of College Student-Consumers by their Lifestyle Types, the Images of Internet Fashion Shopping Malls, and the Purchasing Behaviors)

  • 김경희
    • 한국의류산업학회지
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    • 제10권2호
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    • pp.198-208
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    • 2008
  • The study applies lifestyles based on the list of values(LOV) to the college student-consumers, classifies them and compares traits. The study also looks into store images of internet fashion shopping malls and differences in purchasing behaviors. The results of the study show that there are three types, namely 'self-confidence and pursuit-of-success type,'family-oriented and responsible type', and 'passive and popular-culture-consumer type'. Among the elements constituting the store images of internet fashion shopping malls, there were considerable differences between groups on 'product and information service' element and 'convenience' element. On shopping mall purchasing behavior, the study confirmed some differences on 'information source', 'product purchase amount', and 'payment method' when purchasing a product. Furthermore, there were considerable differences on shopping mall satisfaction level and repurchasing intention between groups by lifestyle-type.

Vegetarian Diets and Estrogen Metabolism in Korean Premenopausal Women

  • Kim Kyung Mi;Sung Mi Kyung
    • Journal of Community Nutrition
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    • 제7권1호
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    • pp.29-35
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    • 2005
  • It has been suggested that dietary fat increases the risk of breast cancer by elevating serum estrogen concentrations. However, studies on a relationship between fat intake and breast cancer risk have shown contradictory results, possibly because the levels of fat intake in study populations were too high to observe differences. Also, the effect of other dietary factors may present. The present study was performed to investigate the relationship between diet and estrogen metabolism in premenopausal women whose usual fat intake is relatively low compared to their western counterparts. Twenty lacto-ovo vegetarians (LOV) and twenty omnivores participated in the study. Three day food records including a Saturday or a Sunday were used to estimate nutrient intake. Serum lipids, estradiol, sex-hormone binding globulin, and urinary estradiol were measured. Study results showed $24.8\%$ and $20.9\%$ of energy intake were provided from fat in omnivorous and LOV subjects, respectively. Serum and urinary levels of estradiol were two times higher in omnivores. Fat intake was not related to either serum estradiol nor urinary estradiol when the Spearman correlation coefficient analysis was performed. Carbohydrate, total dietary fiber and soluble dietary fiber intakes were negatively related to serum estradiol concentration. Legumes, vegetables and fruit consumption showed significantly negative relationships with both serum and urinary estradiol concentrations. These results indicate lower estrogen availability may be associated with plant food­based diets in premenopausal women whose usual diets contain less than $25\%$ of energy as fat.