• Title/Summary/Keyword: LOV

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A New-Generation Fluorescent-Based Metal Sensor - iLOV Protein

  • Ravikumar, Yuvaraj;Nadarajan, Saravanan Prabhu;Lee, Chong-Soon;Rhee, Jin-Kyu;Yun, Hyungdon
    • Journal of Microbiology and Biotechnology
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    • v.25 no.4
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    • pp.503-510
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    • 2015
  • The iLOV protein belongs to a family of blue-light photoreceptor proteins containing a light-oxygen-voltage sensing domain with a noncovalently bound flavin mononucleotide (FMN) as its chromophore. Owing to advantages such as its small size, oxygen-independent nature, and pH stability, iLOV is an ideal candidate over other reporter fluorescent proteins such as GFP and DsRed. Here, for the first time, we describe the feasibility of applying LOV domain-based fluorescent iLOV as a metal sensor by measuring the fluorescence quenching of a protein with respect to the concentration of metal ions. In the present study, we demonstrated the inherent copper sensing property of the iLOV protein and identified the possible amino acids responsible for metal binding. The fluorescence quenching upon exposure to Cu2+ was highly sensitive and exhibited reversibility upon the addition of the metal chelator EDTA. The copper binding constant was found to be 4.72 ± 0.84 µM. In addition, Cu2+-bound iLOV showed high fluorescence quenching at near physiological pH. Further computational analysis yielded a better insight into understanding the possible amino acids responsible for Cu2+ binding with the iLOV protein.

Comparison of Nutrient Intakes, Bone Metabolism and Risk Factors of Chronic Diseases between Postmenopausal Lacto-Ovo-Vegetarian and Omnivores in Kyunggi-Do (일부 폐경기여성의 채식군과 일반식군의 영양섭취상태, 골대사 및 만성 퇴행성 질환의 위험인자에 관한 비교연구)

  • 최선혜;승정자;김미현;이숙연;송숙자
    • Korean Journal of Community Nutrition
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    • v.4 no.3
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    • pp.412-420
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    • 1999
  • The amount and type of dietary protein that effects affect bone mineral loss after menopause and different dietary patterns may influence anthropometric measurements, biochemical values, and bone metabolism. Objective : Our objective was to investigate bone metabolism and chronic disease factors in two different dietary patterns. Design: The sample consisted of 40 lacto-ove-vegetarians(LOV) and 49 omnivores aged from 47 to 85 years in good condition without any medical problems. Results : The average energy intake of LOV was 1414.0kcal and omnivores, 1403.2kcal. The difference between means was not statistically significant. The differences of means of body weight and BMI between the two groups were not significant but the systolic blood pressure of LOV group was significantly lower than omniovores(p<0.05). The values of serum cholesterol(p<0.05) and glucose(p<0.05) of LOV were signifcantly lower than that of omnivores. The serum albumin level(p<0.01) of LOV was lower than it'scounterpart but in a normal range. The intake of crude fiber(p<0.05) of LOV group was significantly higher. The urinary pH of LOV was 7.4 and that of omnivores was 6.3(p<0.001). Plant calcium(p>0.05) and plant iron(p<0.001) had positive correlations with the uninary pH but animal protein(p>0.05) and animal iron(p>0.05) had negative correlations with the urinary pH. The level of urinary biochemical marker of bone resorption, deoxypyridinoline(DPD) of LOV was significantly lower than the other group(p>0.05). The T-scores of bone mineral density for the two sites in the two groups were not significantly different. Conclusions : The DPD level and urinary pH value of LOV were more desirable for the bone metabolism that the omnivores. The diet of LOV had lower risk factors of chronic diseases than the other group.

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The Segmentation of Value System Used by LOV (LOV를 이용한 가치(價値)시스템 세분화(細分化))

  • Shim, Jong-Seop
    • Korean Business Review
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    • v.14
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    • pp.129-148
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    • 2001
  • The purpose of this study is to survey the LOV(List Of Values) composed with the consumer's psychographic variables and is to segment consumer's value system through the LOV. Referring to the previous studies such as Rokeach's RVS, Kahke's LOV, Razzaque's Asian values, and author's Korean values, this study conducted researches of consumer's value segmentation. The result of this study finds that the Korean consumer's value system is categorized into (1) value of influence, (2) value of mammonism, (3) value of latest, (4) value of venture, (5) value of salvation, (6) value of sociality, (7) value of curiosity, (8) spirituality, and (9) value of conservativeness in order. This results will help contribute toward segmentation of Korean consumer's value. Another result of this study shows by way of ANOVA(analysis of variance) that the Korean consumer's value dimensions are closely related to demographic variables: sex, marital status, education, occupation, total families income by monthly, family structure, and age.

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Proof Algorithm of Erdös-Faber-Lovász Conjecture (Erdös-Faber-Lovász 추측 증명 알고리즘)

  • Lee, Sang-Un
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.15 no.1
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    • pp.269-276
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    • 2015
  • This paper proves the Erd$\ddot{o}$s-Faber-Lov$\acute{a}$sz conjecture of the vertex coloring problem, which is so far unresolved. The Erd$\ddot{o}$s-Faber-Lov$\acute{a}$sz conjecture states that "the union of k copies of k-cliques intersecting in at most one vertex pairwise is k-chromatic." i.e., x(G)=k. In a bid to prove this conjecture, this paper employs a method in which it determines the number of intersecting vertices and that of cliques that intersect at one vertex so as to count a vertex of the minimum degree ${\delta}(G)$ in the Minimum Independent Set (MIS) if both the numbers are even and to count a vertex of the maximum degree ${\Delta}(G)$ in otherwise. As a result of this algorithm, the number of MIS obtained is x(G)=k. When applied to $K_k$-clique sum intersecting graphs wherein $3{\leq}k{\leq}8$, the proposed method has proved to be successful in obtaining x(G)=k in all of them. To conclude, the Erd$\ddot{o}$s-Faber-Lov$\acute{a}$sz conjecture implying that "the k-number of $K_k$-clique sum intersecting graph is k-chromatic" is proven.

ESTABLISHMENT OF A MAINTENANCE PROGRAM TO PREVENT LOSS OF OFFSITE POWER IN NUCLEAR POWER PLANTS

  • Lee, Eun-Chan;Na, Jang-Hwan
    • Nuclear Engineering and Technology
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    • v.45 no.6
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    • pp.791-794
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    • 2013
  • Since the Fukushima accident in 2011, the importance of the electrical systems in nuclear power plants (NPPs) has been emphasized. The result has been that NPP regulators are enhancing their monitoring of loss of offsite power (LOOP) events. Korea Hydro & Nuclear Power Co. (KHNP) is reviewing the status and issues related to LOOPs, and is attempting to establish specific countermeasures to prevent LOOPs, because they can have severe consequences in the complicated maintenance schedule during an outage. A starting point for preventing LOOPs is the control of the loss of voltage (LOV)-initiating components. In order to reflect this in the risk assessment program, an LOV monitor is being developed for use during plant outages.

Deploying Linked Open Vocabulary (LOV) to Enhance Library Linked Data

  • Oh, Sam Gyun;Yi, Myongho;Jang, Wonghong
    • Journal of Information Science Theory and Practice
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    • v.3 no.2
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    • pp.6-15
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    • 2015
  • Since the advent of Linked Data (LD) as a method for building webs of data, there have been many attempts to apply and implement LD in various settings. Efforts have been made to convert bibliographic data in libraries into Linked Data, thereby generating Library Linked Data (LLD). However, when memory institutions have tried to link their data with external sources based on principles suggested by Tim Berners-Lee, identifying appropriate vocabularies for use in describing their bibliographic data has proved challenging. The objective of this paper is to discuss the potential role of Linked Open Vocabularies (LOV) in providing better access to various open datasets and facilitating effective linking. The paper will also examine the ways in which memory institutions can utilize LOV to enhance the quality of LLD and LLD-based ontology design.

Study on the Traits of College Student-Consumers by their Lifestyle Types, the Images of Internet Fashion Shopping Malls, and the Purchasing Behaviors (대학생소비자의 라이프스타일 유형별 특성, 인터넷 패션쇼핑몰 이미지 및 구매행동에 관한 연구)

  • Kim, Kyung-Hee
    • Fashion & Textile Research Journal
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    • v.10 no.2
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    • pp.198-208
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    • 2008
  • The study applies lifestyles based on the list of values(LOV) to the college student-consumers, classifies them and compares traits. The study also looks into store images of internet fashion shopping malls and differences in purchasing behaviors. The results of the study show that there are three types, namely 'self-confidence and pursuit-of-success type,'family-oriented and responsible type', and 'passive and popular-culture-consumer type'. Among the elements constituting the store images of internet fashion shopping malls, there were considerable differences between groups on 'product and information service' element and 'convenience' element. On shopping mall purchasing behavior, the study confirmed some differences on 'information source', 'product purchase amount', and 'payment method' when purchasing a product. Furthermore, there were considerable differences on shopping mall satisfaction level and repurchasing intention between groups by lifestyle-type.

Vegetarian Diets and Estrogen Metabolism in Korean Premenopausal Women

  • Kim Kyung Mi;Sung Mi Kyung
    • Journal of Community Nutrition
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    • v.7 no.1
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    • pp.29-35
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    • 2005
  • It has been suggested that dietary fat increases the risk of breast cancer by elevating serum estrogen concentrations. However, studies on a relationship between fat intake and breast cancer risk have shown contradictory results, possibly because the levels of fat intake in study populations were too high to observe differences. Also, the effect of other dietary factors may present. The present study was performed to investigate the relationship between diet and estrogen metabolism in premenopausal women whose usual fat intake is relatively low compared to their western counterparts. Twenty lacto-ovo vegetarians (LOV) and twenty omnivores participated in the study. Three day food records including a Saturday or a Sunday were used to estimate nutrient intake. Serum lipids, estradiol, sex-hormone binding globulin, and urinary estradiol were measured. Study results showed $24.8\%$ and $20.9\%$ of energy intake were provided from fat in omnivorous and LOV subjects, respectively. Serum and urinary levels of estradiol were two times higher in omnivores. Fat intake was not related to either serum estradiol nor urinary estradiol when the Spearman correlation coefficient analysis was performed. Carbohydrate, total dietary fiber and soluble dietary fiber intakes were negatively related to serum estradiol concentration. Legumes, vegetables and fruit consumption showed significantly negative relationships with both serum and urinary estradiol concentrations. These results indicate lower estrogen availability may be associated with plant food­based diets in premenopausal women whose usual diets contain less than $25\%$ of energy as fat.