• Title/Summary/Keyword: LOCUST

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Improvement of Black Locust(Robinia pseudoacacia L.) Through Tissue Culture. I. Micropropagation and Somatic Embryogenesis (조직배양에 의한 아까시나무(Robinia pseudoacacia L.)의 개량 I. 대량증식과 체세포배 발생)

  • Woo, Jong Ho;Choi, Myung Suk;Joung, Eun Yi;Chung, Won Il;Jo, Jin Ki;Park, Young Goo
    • Journal of Korean Society of Forest Science
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    • v.84 no.1
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    • pp.41-47
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    • 1995
  • A micropropagation system for black Locust(Robinia pseudoacacia) was established by using shoots and pin-punctured leaves of in vitro germinated seedlings. The greatest number of shoots (an average of 10.5 shoots) was obtained when shoot tips were cultured on MS medium supplemented with 1.0 mg/l BAP and 0.01 mg/l NAA. When pin-punctured leaf explants were cultured on the same medium, mean number of 13.5 shoots were produced. Shoot growth was accelerated by adding 50 mg/l of silver nitrate ($AgNO_3$), an anti-ethylene compound to the culture medium. Each shoot was excised from the mass and transferred onto half strength MS medium for rooting. Zygotic embryos at different developmental stages were cultured on LS medium supplemented with various growth regulators to induce somatic embryos. When cultured on LS medium with 1.0 mg/l 2,4-D. 14.3% of the zygotic embryos induced somatic embryos. Upon transfer onto the basal medium, somatic embryos sporadically converted into plantlets.

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Allopathic Effect of Black Locust (Robinia pseudoacacia L.) and Analysis for Its Allelochemicals

  • Lee, Chul;Park, Ju-Yong;Kim, Jung-Hee;Park, Young-Goo
    • Journal of Korea Foresty Energy
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    • v.22 no.3
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    • pp.11-19
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    • 2003
  • Allelopathic effects of black locust(Robinia pseudoacacia L.) was evaluated through germination tests using 13 different species including R. pseudoacacia itself. The water extract from leaf or root of R. pseudoacacia was separately tested. Seed germination of several species was inhibited in the presence of leaf or root extract, although the level of germination was in a wide range depending on the species. Seed germination of R. pseudoacacia was suppressed by 30 % in its leaf extract. Among tested tree species, Hibiscus syriacus and Alnus frma showed significantly inhibited germination in the root extracts whereas Thuja orientalis exhibited germination inhibition in the leaf extract. HPLC was carried out to identify allelochemicals in extracts of leaf and root using eight authentic phenolic compounds that are known to be responsible for allelopathy. The major phenolic compounds occupying about 40 % of total phenolic acids were gentisic acid in leaves and vanillic acid and syringic acid in roots. The leaves contained 7 times more phenolic acids than the roots. Among 8 tested phenolic compounds vanillin was detected only in roots and coumaric acid in leaves. Consequently, 3 out of 13 species showed significantly inhibited germination due to leaf or root extract. This germination test indicates that the inhibitory allelopathic effect by R. pseudoacacia is caused by chemical interaction not by nutritional competition and that the allelochermicals of black locust act species-specifically. No specific influence of the total amount of phenolic compounds on the allelopathic inhibitory germination and the synergic effect by each phenolic compound may play a role for the allelopathic effect by R. pseudoacacia.

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Impact of Xanthan-locust Bean Gum Mixtures on Pasting/Paste Characteristics and Freeze-thaw Stabilities of Waxy Rice Starch (찹쌀 전분의 페이스팅/페이스트 특성 및 냉해동 안정성에 대한 잔탄검-로커스트콩검 혼합물의 영향)

  • Kim, Hyun-Seok
    • Korean Journal of Food Science and Technology
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    • v.46 no.5
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    • pp.593-600
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    • 2014
  • Normal rice starch (NRS) possesses high gelling and retrogradation tendencies, with poor freeze-thaw stability. This study investigated the effects of partial replacement of waxy rice starch (WRS) with gums on the pasting and viscoelastic properties as well as the freeze-thaw stability of the WRS paste. Xanthan gum (XAT), locust bean gum (LBG), and their mixtures were individually mixed with WRS at a ratio of 1:19 (w/w). WRS-gum mixtures were pasted using a rapid visco-analyzer at 5% total solid content, and analyzed with respect to the pasting and viscoelastic characteristics, and freeze-thaw stability. Pasting properties of WRS were retarded in pasting temperature and enhanced in pasting viscosity (although peak viscosity was varied) by partial replacement with gum and gum mixtures. Storage moduli of WRS-XAT:LBG pastes became similar to those of NRS paste with increasing angular frequency from 1 to 10 rad/s. Finally, WRS-XAT and WRS-XAT:LBG possessed more enhanced freeze-thaw stability than NRS.

Xanthan gum의 생산과 응용

  • 정동효
    • Food Industry
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    • s.45
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    • pp.32-37
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    • 1978
  • 자연에서 얻어지는 고무질로서는 여러 가지 종류의 것이 많으나 한천, 알긴산염, 카라기난(Carrageenan), 퍼셀라란(furcellaran)등은 해조로부터 추출되며 Pectin, arabic gum, karaya gum, tragacanth gum, ghatti gum, guargum, 메뚜기콩고무(Locust been gum), 사이리엄고무(psyllium seed gum)등은 식물로부터 추출된다. 특히 이외에도 미생물에서 분비되는 고무질인 다당(多糖)으로는 dextran, curdlan, pullulau, 잔산고무(Xanthan gum)등도 최근에 알려진 고무질이다. 여기에서는 미생물이 생산하는 고무질인 다당(多糖)인 Xanthan gum이 새로히 식품첨가물로 지정되었기에 Xanthan gum의 발견내역, 성질, 생산과 응용면을 소개한다.

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다당류를 이용한 중금속 이온의 흡착특성 비교

  • Kim, Se-Gyeong;Yun, Hyeon-Sik
    • 한국생물공학회:학술대회논문집
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    • 2000.04a
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    • pp.386-389
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    • 2000
  • Polysaccharides are very effective adsorbents for heavy metals. In this study, the adsorption characteristics of various polysaccharides for heavy metal adsorption were investigated. Tested polysaccharides were homogeneous polysaccharides such as curdlan, chitin, starch, cellulose, Avicel, and Solka floc and heterogeneous polysaccharides such as zooglan, locust bean gum, ghatti gum, pectin, and xylan. Lead(II) adsoption characteristic on these polysaccharides followed Freundlich isotherm and the isotherm parameters were calculated. For adsorption of lead(ll), Avicel, starch, and zooglan were found to be good adsorbents.

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Viscosity of Exopolysaccharide from Xanthomonas sp. EPS-1 (Xanthomonas sp. EPS-1이 생산하는 다당류의 점도)

  • 손봉수;박석규;이상원;성찬기;서권일
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.25 no.1
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    • pp.53-57
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    • 1996
  • Xanthomonas sp. EPS-1으로부터 생산된 다당류 EPS-1 용액의 농도가 0.04(g/dl)일 때, 비점도는 0.137, 환원 점도는 3.425, 상대점도는 1.137, 고유점도는 3.209임을 알 수 있었다. 겉보기 점도는 온도상슴에 따라 낮아졌으나 다시 온도를 증가하였을때는 서서히 증가하였다. 열처리 후에도 물성은 거의 변화지 않았으며, 응집성은 좋지 않았으나 locust bean gum과의 혼합효과는 우수하였다.

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Production and Properties of Mannanase and Xylanase by a Bacillus subtilis Isolate (Bacillus subtilis 분리균의 Mannanase와 Xylanase 생산성과 효소 특성)

  • Yoon, Ki-Hong
    • Microbiology and Biotechnology Letters
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    • v.43 no.3
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    • pp.204-211
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    • 2015
  • A bacterial strain capable of hydrolyzing xylan and locust bean gum (LBG) was isolated from the Saemangeum tideland of Korea. Based on the biochemical properties and the 16S rRNA gene sequence, the isolate YB-30 was identified as Bacillus subtilis. Xylanase productivity was increased effectively when B. subtilis YB-30 was grown in the presence of wheat bran, while mannanase productivity was increased drastically when grown in the presence of konjac or LBG. Particularly, maximum mannanase and xylanase activities were detected in the culture filtrate of media containing 3.5% konjac and 1% wheat bran. Both enzyme productivities reached maximum levels in the stationary growth phase. The culture filtrate exhibited the highest activity at 60℃ and pH 6.0 for mannanase and at 55℃ and pH 5.5 for xylanase, respectively. Both enzymes were not stable at high temperatures and xylanase was less stable than mannanase. In addition, wheat bran was hydrolyzed to liberate reducing sugar to a greater extent than rice bran by the culture filtrate because the wheat bran contained more arabinoxylan than the rice bran. Hence, xylanase and mannanase produced by B. subtilis YB-30 have a potential use as feed additive enzymes.

Landscape Structure in the Greenbelt Zone around the Seoul, the Metropolis of Korea

  • Lee, Chang-Seok;Hong, Sun-Kee;Moon, Jeong-Suk;You, Young-Han
    • The Korean Journal of Ecology
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    • v.24 no.6
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    • pp.385-394
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    • 2001
  • An attempt to clarify the landscape structure of urban areas was carried out in the greenbelt around Seoul, Korea's metropolis. By means of aerial photographs and a field survey, a vegetation map including land-use pattern was made. Landscape structure was described by analyzing this vegetation map and the results of phytosociological survey. Landscape element types identified were (1) secondary forest, (2) plantation, (3) cultivated field, (4) urbanized area, (5) graveyard, and (6) bare rock. Vegetation units, resulting from the phytosociological analysis, included Quercus mongolica, Q. variabilis, Q. acutissima, Pinus densiflora, Q. aliena, and Alnus japonica communities. Plantations were composed of Robinia pseudoacacia, Populus tomentiglandulosa, P. rigida, Larix leptolepis, P. koraiensis, and Castanea crenata stands. Patches near to human settlements in the lower zones of the mountains were fragmented and small but they became larger towards the higher mountain zones. On the other hand, the number of patches was fewer and their size was larger in Mt. Cheonggye more distant from the principal residential area, larger in size, and higher in elevation compared with the other 2 mountains, Mt. Daemo and Mt. Acha. Floristic composition of Mongolian oak(Q. mongolica) stand distributing in the upper part of each mountain, in which artificial interference is rare, showed a difference among those study areas different in parent rock and disturbance regime. But that of black locust(R. pseudoacacia) stand located in lowland of mountainous area, in which artificial interference is frequent was similar to each other. As the results of analyses on the frequency distribution of diameter classes of major species, dominant landscape elements, Mongolian oak forest showed different responses depending on artificial interference as continuous maintenance and retrogressive succession in the sites far from and near to the residential areas, respectively. On the other hand, black locust stands showed a probability to be restore to the native oak forest through progressive succession.

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