• Journal of Life Science
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• v.24 no.6
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• pp.633-641
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• 2014

This study investigated the antioxidant and antiobesity activity of extract powders from the following natural color resources: Polygonum indigo, Black locust, Cochineal, Catechu, Grape, Tesu flower, Henna, Chrysanthemum, Sandalwood Red, Himalayan Rhubarb, and Madder. Total phenol content was the highest in Catechu extract, at 348.25 mg/g. DPPH, ABTS radical scavenging activity and ferric reducing antioxidant power (FRAP) were also higher in Catechu extract. Bleaching inhibition activity in the ${\beta}$-carotene linoleic acid system was the highest in Black locust extract, as was ${\alpha}$-Glucosidase inhibition activity. ${\alpha}$-Amylase inhibition activity was the highest in Catechu extract. Trypsin inhibition activity of Black locust extract was greater than 60%, and ${\alpha}$- chymotrypsin inhibition activity of Catechu extract was greater than 40%. Lipase inhibition activity was the highest Black locust extract, at 52.73%. Viability of 3T3-L1 cells was not affected by treatment with extracts at concentrations of $1.25{\sim}25{\mu}g/ml$. Lipid accumulation in the 3T3-L1 cells was the lowest following treatment with Catechu extract, at 55.8%, and this extract also inhibited adipocyte differentiation. These results suggest that the Catechu and Black locust extracts have high antioxidant and antiobesity activities and can be useful ingredients in functional foods.

• Korean Journal of Plant Resources
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• v.36 no.1
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• pp.91-99
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• 2023

Plus tree selection is the essential first step in a tree improvement program. This study was carried out to select plus trees of black locust (Robinia pseudoacacia L.) for tree improvement in Korea. The candidate plus trees of black locust were selected from twenty-one places in the four provinces (Gangwon, Gyeonggi, Chungcheong and Gyeongsang) following comparison selection. The trees were examined for qualitative and quantitative traits of economic interest like tree height (H), diameter at breast height (DBH), clear bole height (CBH), stem straightness (SS), crown size (CS) and disease resistance. The 64 selected trees showed higher quality in growth characteristics than control trees. Average of height, DBH, CBH, SS and CS were 20.5 m, 31.1 cm, 10.1 m, 1.2 and 5.2 m respectively. In particular, it was shown that the growth characteristics were higher in Chungcheong population. The superiority percentage of selected candidate plus trees of black locust over their respective check trees ranged from 7.3% to 18.9% in height, 20.0% to 40.6% in DBH, 32.8% to 73.5% in CBH, 42.9% to 55.2% in SS and -11.1% to 9.4% in CS, respectively. The selected black locust will be useful in future tree breeding programs based on their clonal and progeny performance.

• Journal of Korean Society of Forest Science
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• v.112 no.1
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• pp.11-22
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• 2023

This study was established to provide basic information necessary for ecological management to restore the naturalness of black locust (Robinia pseudoacacia L.) plantations located in the mountains of Gyeongbuk, Korea. Using vegetation data collected from 200 black locust stands, vegetation types were classified using the TWINSPAN method, the spatial arrangement status according to the environmental gradient was identified through DCA analysis, and a synoptic table of communities was prepared based on the diagnostic species determined by determining community fidelity (Φ) for each vegetation type. The vegetation types were classified into seven types, namely, Quercus mongolica-Polygonatum odoratum var. pluriflorum type, Castanea crenata-Smilax china type, Clematis apiifolia-Lonicera japonica type, Rosa multiflora-Artemisia indica type, Quercus variabilis-Lindera glauca type, Ulmus parvifolia-Celtis sinensis type, and Prunus padus-Celastrus flagellaris type. These types usually reflected differences in complex factors such as altitude, moisture regime, successional stage, and disturbance regime. The mean relative importance value of the constituent species was highest for black locust(39.7), but oaks such as Quercus variabilis, Q. serrata, Q. mongolica, Q. acutissima, and Q. aliena were also identified as important constituent species with high relative importance values, indicating their potential for successional trends. In addition, the total percent cover of constituent species by vegetation type, life form composition, species diversity index, and indicator species were compared.

• Korean journal of applied entomology
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• v.56 no.3
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• pp.301-308
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• 2017

Locusts, Locusta migratoria (Orthoptera: Acrididae) are periodical unpredictable agricultural pests worldwide and cause serious damage to crop production; however, little consideration has been given to the management of this pest. Herein, we constructed a locust-pathogenic fungal library and confirmed that some fungi could be used as resources for locust management. First, the entomopathogenic fungi were collected from sampled soils using a Tenebrio molitor-based baiting system. For the locust assay, a locust colony was obtained from the National Institute of Agricultural Science and Technology. A total of 34 entomopathogenic fungal granules, which were produced by solid cultures, were placed in the plastic insect-rearing boxes (2 g/box) and nymphs of locust were contained in the box. In 3-7 days, mycosis was observed on the membranous cuticles of the head, abdomen, and legs of locusts. In particular, Metarhizium anisopliae, M. lepidiotae, and Clonostachys rogersoniana exhibited high virulence against the locust. Given that the 34 isolates could be used in field applications, their conidial production and stability (thermotolerance) were further characterized. In the thermotolerance assay, Paecilomyces and Purpureocillium isolates had higher thermotolerance than the other isolates. Most of the fungal isolates produced ca. >$1{\times}10^8conidia/g$ on millet grain medium. In a greenhouse trial, the granular application of M. anisopliae isolate on the soil surface resulted in 85.7% control efficacy. This work suggests that entomopathogenic fungi in a granular form can be effectively used to control the migratory locust.

• Journal of the Korean Wood Science and Technology
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• v.29 no.3
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• pp.21-26
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• 2001

Acquisition of precise information on drying characteristics of wood is indispensable for the improvement of drying schedules and wood quality. Recognition of the exact moisture content at which drying defects such as checks occur during drying with given drying conditions may be essential to reduce drying losses. In this study an image-processing system was combined with a laboratory-scale wood dry kiln for experiments and the surface of tree disk of black locust (Robinia pseudoacacia L.) was monitored to investigate the behavior of check formation over all the drying process. This system showed good potential for improving drying schedules and wood product quality.

• Journal of the Korean Wood Science and Technology
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• v.26 no.4
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• pp.34-42
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• 1998

Three hardwood (ash, alder and black locust) and three softwood (Japanese red pine, radiata pine and Western hemlock) specimens were dried in microwave(MlW) oven and their drying rates were obtained. Their specific permeabilities were also measured by using a modified liquid permeability measuring device. The correlation between the M/W drying rates and permeabilities of six species were statistically analyzed. It was revealed that within a species there is a logarithmic relationship between the M/W drying rates and average moisture contents and that among species there is a linear relationship between the M/W maximum drying rates and the average specific permeabilities. A exception was Western hemlock, which was of low permeability and of high drying rate. A Me-time equation, which showed a good agreement with the actual data, was derived. Thus using this equation the moisture contents of wood and the drying end points of M/W drying could be predicted. Infrared images of the thermal distribution in wood were illustrated.

• Korean Journal of Food Science and Technology
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• v.49 no.2
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• pp.181-185
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• 2017

Senior-friendly strawberry jelly was developed using strawberry juice, sugar, xanthan gum, and locust bean gum. The experimental variables included strawberry juice (30.0-40.0%, w/w) and sugar (7.5-10.0%, w/w) concentrations and the xanthan gum/locust bean gum ratio (0.3-4.0), and interactions among these variables were predicted using a response surface methodology. The optimal concentrations of strawberry juice and sugar and the ratio of xanthan gum/locust bean gum, determined against jelly hardness, were found to be 40.0, 10.0, and 1.5%, respectively. The hardness, gumminess, and chewiness of strawberry jelly increased significantly during storage at 5 and $15^{\circ}C$ (p<0.05). The lightness of the gum decreased significantly at both temperatures (p<0.05), whereas the hue angle increased during storage at $15^{\circ}C$. The zero-order kinetics was used to predict the shelf-life of the prepared jelly ($R^2=0.89-0.96$), which was determined to be 38 and 26 days at 5 and $15^{\circ}C$, respectively.

• Journal of Korean Society for Atmospheric Environment
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• v.23 no.1
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• pp.29-38
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• 2007

Recently, the tropospheric ozone has gained a global attention for its adverse effect on vegetation as well as its contribution to the global warming. Although a number of studies have been carried out for the urban ozone, the effect of ozone on vegetation is still largely unknown in Korea. The present work aimed at performing the first evaluation of forest damage by ozone using the national air monitoring data from the year 2000 to the year 2005. Moreover, it also explored the relevance of adverse effect of ozone to the recent events on leaf chlorosis of black locust, Robinia pseudo-acacia, leading to early foliage widely observed in Korea since 2001. In the nineties, forest damages caused by ozone such as leaf chlorosis occurred in Europe and North America and led to invoke comprehensive field and laboratory studies. As a result, AOT40, implying the accumulated dose over a threshold 40 ppb, was developed to assess the forest damage by ozone. 19 air monitoring stations were selected across Korea to calculate AOT40 from the year 2000 to the year 2005. The calculated AOT40 generally increased during April and May and reached the maximum after May. The increase of AOT40 diminished substantially from the mid-June as the rainy season started. The calculated AOT40 in the nine rural sites from the year 2002 to th ε year 2005 ranged from 6.8 to 29.4 ppm-hr. And all the AOT40 at th ε year 2005 exceeded the critical value of 10 ppm-hr for forest damage. Morevoer the calculated AOT40 of Korea appeared higher than the AOT40 of Europe in the year 2004/2005 and comparable to the AOT40 of China in the year 2000. Despite the high levels of the calculated AOT40 since the year 2001 and the consistency of time of seasons between high AOT40 values and occurrence of chlorosis of black locust, further studies are required to conclude that the chlorosis of black locust occurred from the year 2001 to the year 2005 were resulted from a damage by ozone.

• Microbiology and Biotechnology Letters
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• v.30 no.3
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• pp.247-252
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• 2002

A bacterium producing the extracellular mannanase was isolated from Korean formented food and has been identified as a member of the genus Bacillus from the result of the phylogenic analysis based on partial 165 rRNA sequences. The isolate, named Bacillus sp. WL-3, was shown to be similar to B. subtilis strain on the basis of its biochemical properties. The mannanase of culture supematant was the most active at $55^{\circ}C$ and pH 6.0. The additional carbohydrates including u-cellulose, avicel, oat spelt xylan, guar gum and locust bean gum (LBG) increased the mannanase productivity. Especially, the maximum mannanase productivity was reached 65.5 U/ml in LB medium supplemented with 0.5% (w/v) LBG, which was 131-folds more than that in LB medium. It was sug-gested that the increase of mannanase production was owing to induction of mannanase biosynthesis by LBG hydrolysates transported following initial hydrolysis by extracellular mannanase during the cell growth. The molec-ular weight of WL-3 mannanase was estimated to approximately 38.0 kDa by zymogram on SDS-PAGE.

• Journal of Korean Society of Forest Science
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• v.84 no.2
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• pp.145-150
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• 1995

A plant regeneration system using shoot basal callus of in vitro cultured black locust(Robinia pseudoacacia L.) was established. Shoot basal callus was induced on MS medium supplemented with BA, or NAA, and mere more proliferated on BA containing medium than NAA containing medium at both light and dark conditions. Shoot basal callus was induced during shoot multiplication procedure. Two types of callus, green colored callus and whitish-yellow colored callus, were cultured on mMS medium containing 2.0 mg/l BA and 0.5 mg/l NAA. Green colored callus showed the shoot regeneration ability while whitish-yellow callus failed to regenerate shoot and died. Regenerated shoot were rooted on hormone-free ${\frac{1}{2}}MS$ medium within 2 weeks.