• Asian Pacific Journal of Cancer Prevention
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• v.15 no.19
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• pp.8035-8040
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• 2014

Gastric cancer is the second after lung cause of cancer-related mortality in the world. Early detection and treatment can lead to a long survival time. Recently microarrays and next generation sequencing (NGS) have become very useful tools of comprehensive research into gastric cancer, facilitating the identification of treatment targets and personalized treatments. However, there are numerous challenges from cancer target discovery to practical clinical benefits. Although there are many biomarkers and target agents, only a minority of patients are tested and treated accordingly. Microarray technology with maturity was established more than 10 years ago, and has been widely used in the study of functional genomics, systems biology, and genomes in medicine. Second generation sequencing technology is more recent, but development is very fast, and it has been applied to the genome, including sequencing and epigenetics and many aspects of functional genomics. Here we review insights gained from these studies regarding the technology of microarray and NGS, how to elucidate the molecular basis of gastric cancer and identify potential therapeutic targets, and how to analyse candidate genes. We also discuss the challenges and future directions of such efforts.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.14 no.4
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• pp.1479-1501
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• 2020

Dependable functioning of dynamic networks is essential for delivering ubiquitous services. Faults are the root causes of network outages. The comparison diagnosis model, which automates fault's identification, is one of the leading approaches to attain network dependability. Most of the existing research has focused on stationary networks. Nonetheless, the time-free comparison model imposes no time constraints on the system under considerations, and it suits most of the diagnosis requirements of dynamic networks. This paper presents a novel protocol that diagnoses faulty nodes in diagnosable dynamic networks. The proposed protocol comprises two stages, a testing stage, which uses the time-free comparison model to diagnose faulty neighbour nodes, and a disseminating stage, which leverages a Random Linear Network Coding (RLNC) technique to disseminate the partial view of nodes. We analysed and evaluated the performance of the proposed protocol under various scenarios, considering two metrics: communication overhead and diagnosis time. The simulation results revealed that the proposed protocol diagnoses different types of faults in dynamic networks. Compared with most related protocols, our proposed protocol has very low communication overhead and diagnosis time. These results demonstrated that the proposed protocol is energy-efficient, scalable, and robust.

• Proceedings of the KSME Conference
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• 2002.08a
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• pp.255-258
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• 2002

Liquid rocket engines using liquefied natural gas (LNG) or methane as a fuel is known to have several good characteristics, such as high specific impulse compared to other hydrocarbon fuels, environment-friendly exhaust gas, low production cost, and re-usability with low soot generation in the cooling channel. In this study, experimental combustion chambers capable of using LNC and $CH_{4}$ are being researched through experimental firing tests, and within easy range of eyes' inspection, there are the periodical existence of soot or discoloration in the chamber wall surface. This result means that mixture ratio of oxidizer and fuel fluctuates periodically between outer-row injectors in the mixing head in the circumferential direction. Therefore, based on this phenomenon, the variation of mixture ratio near the chamber wall caused by the spill pattern of a shear coaxial injector was analyzed quantitatively and the thermal heat flux Into the cooling channel is modified. Then, the calculated and modified results are compared with the measured ones.

• BMB Reports
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• v.50 no.4
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• pp.162-163
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• 2017

RNA polymerase II-interacting the Set2 methyltransferase co-transcriptionally methylates histone H3 at lysine 36 within the body of genes. This modification facilitates histone deacetylation by Rpd3S HDAC in 3' transcribed regions to suppress cryptic initiation and slow elongation. Although this pathway is important for global deacetylation, no strong effects have been seen on genome-wide transcription under optimized laboratory conditions. In contrast, this pathway slows the kinetics of mRNA induction when target genes are induced upon environmental changes. Interestingly, a majority of Set2-repressed genes are overlapped by a lncRNA transcription that targets H3K36 methylation and deacetylation by Rpd3S HDAC to mRNA promoters. Furthermore, this pathway delays the induction of many cryptic transcripts upon environmental changes. Therefore, the Set2-Rpd3S HDAC pathway functions to fine-tune expression dynamics of mRNAs and ncRNAs.

• Speech Sciences
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• v.13 no.2
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• pp.87-105
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• 2006

Many researchers have studied the relationship between the information structure and intonation. Arguments about the relations between the information structure and intonation researched so far can be summarized as follows: the intonation of topic and focus in English information structure is represented as i) a pitch accent, ii) a tune (a pitch accent + an edge tone), or iii) a boundary tone. The purpose of this paper is to study various informational patterns of the topic in English information structure, using real TV discussion data. In this paper, the topic is classified as contrastive topics and non-contrastive topics, based on contrastiveness. The results show that the intonation of the topic in English information structure is implemented as a pitch accent, neither a tune nor a boundary tone. Of the non-contrastive topics, while anaphoric determinative NP topics (Lnc, Lncd) are mainly represented as a H* pitch accent, the pronoun topic(Lp) does not have a pitch accent. Of contrastive topics, while the semantically focused topic(Lci) is mainly represented as a H* pitch accent, the contrastively focused topic(Lcc) is represented as both H* and L+H* pitch accents. It shows that it is not always true that the topic or focus to have the meaning of contrast is represented as a L+H* pitch accent as argued in the previous researches.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.6
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• pp.900-904
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• 2002

The duration of spatial memory in cattle is potentially important for grazing management. The ability of livestock to remember the location of food patches may lead to uneven range use. In this experiment, how long cattle are able to remember food locations was determined. Six steers were used to conduct this study in a pasture with an 8 row by 8 column grid of 64 plastic containers 5 meters apart. Four randomly chosen containers were loaded with feed pellets. All steers were trained to find the loaded locations until a minimum of empty containers were visited. After this initial training, each steer was tested at 5, 10, 20 and 48 days post-training. Total number of visits (TV), number of containers visited (NC), the ratio of loaded containers (LC) to NC were recorded. Once the steers learned the locations of loaded containers NC did not increase with time since last training up to 48 days (p>0.05). Logarithmic transformation of NC (LNC) was 0.70 and 0.80 for the control and 48 day treatments, respectively. Steers were equally efficient in locating containers with feed. The steers also showed that their ability in locating food was much better than expected by chance (Z>1.62). Findings of the present study do not suggest using spatial memory decay as a tool to promote better grazing distribution. Because steers remembered food locations accurately for at least 48 days.

• Molecules and Cells
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• v.41 no.2
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• pp.134-139
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• 2018

Here, we report isolation of multiple long non-coding RNAs (lncRNAs) expressed tissue-specifically during murine embryogenesis. One of these, subsequently came to be known as Redrum, is expressed in erythropoietic cells in fetal liver and adult bone marrow. Redrum transcription is also detected during pregnancy in the spleen where extramedullary hematopoiesis takes place. In order to examine the function of Redrum in vivo, we generated a gene-targeted murine model and analyzed its embryonic and adult erythropoiesis. The homozygous mutant embryo showed no apparent deficiency or defect in erythropoiesis. Adult erythropoiesis in bone marrow and in the spleen during pregnancy likewise showed no detectable phenotype as red blood cells matured in normal fashion. The phenotype is in contrast to the reported function of Redrum in vitro, and our observation implies that Redrum plays in vivo an accessory or supplementary role whose loss is compatible with normal erythropoiesis.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.17 no.6
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• pp.98-104
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• 2018

Deflected 4-way panels of ceiling air conditioners produced by injection molding process have caused dew condensation at the edge of products. In order to prevent this drawback with reducing weight and deformation, this study proposed renovated process adopting microcellular foaming. According to results from 2-sample t-test and analysis of variance(ANOVA), the critical factors affecting weight were melt temperature and injection speed. In addition, the vital effects on deformation were structure at the edge, mold temperature and cooling time. Optimal conditions of these parameters were derived by regressive analysis with CAE and response surface method(RSM), and then applied to an actual design and process stage to analyze performance. As a results, it clearly showed that new process improved process capability as well as reduced both weight and deformation by 18.8% and 71.9% respectively compared to the conventional method.

• BMB Reports
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• v.52 no.1
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• pp.86-108
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• 2019

In multi-cellular organisms, the control of gene expression is key not only for development, but also for adult cellular homeostasis, and gene expression has been observed to be deregulated with aging. In this review, we discuss the current knowledge on the transcriptional alterations that have been described to occur with age in metazoans. First, we discuss age-related transcriptional changes in protein-coding genes, the expected functional impact of such changes, and how known pro-longevity interventions impact these changes. Second, we discuss the changes and impact of emerging aspects of transcription in aging, including age-related changes in splicing, lncRNAs and circRNAs. Third, we discuss the changes and potential impact of transcription of transposable elements with aging. Fourth, we highlight small ncRNAs and their potential impact on the regulation of aging phenotypes. Understanding the aging transcriptome will be key to identify important regulatory targets, and ultimately slow-down or reverse aging and extend healthy lifespan in humans.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.7
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• pp.2851-2855
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• 2015

Background: Recent reports have shown that nuclear enriched abundant transcript 1 (NEAT1), a long noncoding RNA (lncRNA), contributes to the precise control of gene expression and is related to several human malignancies. However, limited data are available on the expression and function of NEAT1 in lung cancer. The major objective of the current study was to profile the expression and clinicopathological significance of NEAT1 in non-small cell lung cancers (NSCLCs). Materials and Methods: NEAT1 expression in 125 NSCLC cases and paired adjacent non-cancer tissues was assessed by real-time quantitative reverse transcription-PCR (qRT-PCR). Relationships between NEAT1 and clinicopathological factors were also investigated. Results: The relative level of NEAT1 was $6.98{\pm}3.74$ in NSCLC tissues, significantly elevated as compared to that of the adjacent non-cancer lung tissues ($4.83{\pm}2.98$, p<0.001). The area under curve (AUC) of high expression of NEAT1 to diagnose NSCLC was 0.684 (95% CI: 0.619~0.750, p<0.001). NEAT1 expression was positively correlated with patient age (r=-2.007, p=0.047), lymphatic metastasis (r=-2.731, p=0.007), vascular invasion (r=-3.617, p=0.001) and clinical TNM stage (r=-4.134, p<0.001). Conclusions: This study indicates that NEAT1 might be associated with oncogenesis and progression in NSCLC, and suggests application in molecular targeted therapy.