• Asian Pacific Journal of Cancer Prevention
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• v.15 no.23
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• pp.10299-10306
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• 2015

The esophageal squamous cell carcinoma (ESCC) is thought to develop through a multi-stage process. Epigenetic gene silencing constitutes an alternative or complementary mechanism to mutational events in tumorigenesis. Posttranscriptional regulation of human leukocyte antigen class I (HLA-I) and antigen processing machinery (APM) proteins expression may be associated with novel epigenetic modifications in cancer development. In the present study, we determined the expression levels of HLA-I antigen and APM components by immunohistochemistry. Then by a bisulfite-sequencing PCR (BSP) approach, we identified target CpG islands methylated at the gene promoter region of APM family genes in a ESCC cell line (ECa109), and further quantitative analysis of CpG site specific methylation of these genes in cases of Kazakh primary ESCCs with corresponding non-cancerous esophageal tissues using the Sequenom MassARRAY platform. Here we showed that the development of ESCCs was accompanied by partial or total loss of protein expression of HLA-B, TAP2, LMP7, tapasin and ERp57. The results demonstrated that although no statistical significance was found of global target CpG fragment methylation level sof HLA-B, TAP2, tapasin and ERp57 genes between ESCC and corresponding non-cancerous esophageal tissues, there was significant differences in the methylation level of several single sites between the two groups. Of thesse only the global methylation level of LMP7 gene target fragments was statistically higher ($0.0517{\pm}0.0357$) in Kazakh esophageal cancer than in neighboring normal tissues ($0.0380{\pm}0.0214$, p<0.05). Our results suggest that multiple CpG sites, but not methylation of every site leads to down regulation or deletion of gene expression. Only some of them result in genetic transcription, and silencing of HLA-B, ERp57, and LMP7 expression through hypermethylation of the promoters or other mechanisms may contribute to mechanisms of tumor escape from immune surveillance in Kazakh esophageal carcinogenesis.

• Journal of Microbiology and Biotechnology
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• v.10 no.5
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• pp.620-627
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• 2000

To determine the prevalence of genetic polymorphisms in Epstein-Barr virus (EBV) strains in the Korean population, the restriction site polymorphisms for BamHI and XhoI enzymes were analyzed with 16 EBV isolates from cancer patients and 7 EBV isolates from healthy carriers, using polymerase chain reaction techniques. None of the 23 isolates were found to carry an extra BamHI site in the BamHI F-fragment (f-variant). Of the 12 type-1 isolates from the cancer patients, 10 lost both the LMP1 XhoI site and the BamHI site between the BamHi W1* and I1* fragments (a W1*I1* fusion variant or type C). The latter W1*I1* fusion variant was due to a mutation of thymidine to adenine, as evidenced by a sequence analysis. The remaining two type-1 isolates showed either no variation at both sites or the loss of only the XhoI site. In contrast, two type-2 isolates and two intertypic recombinants with a type-1 allele at the EBNA2 locus and type-2 alleles at all or some of the EBNA3 loci retained both enzyme sites. In similar analyses of the 7 isolates from the healthy carriers, five of six type-1 isolates lost these two sites, however, one type-2 isolate did not. These results clearly indicate a strong association of both the LMP1 XhoI site loss and the W1*I1* fusion variant with the type-1 rather than the type-2 EBV strains circulating in the immunocompetent Korean carriers.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2002.05a
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• pp.191-192
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• 2002

• Journal of the Korea Institute of Information and Communication Engineering
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• v.7 no.5
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• pp.925-933
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• 2003

Network is expected to be developed into optical Internet network collected IP layer and optical layer, but GMPLS is risen at the transitional evolution stage because of the present technology level. GMPLS that MPLS is extended and generalized is able to support not only the packet switching device but also the devices which perform switching in time, wavelength, and space domain. To implement the common control plane to these various switching types, GMPLS extends the existing MPLS signaling and routing protocol. In this paper, we describe the overview of GMPLS technology, and then we will refer to the OSPF(Open Shortest Path First), which was used to exchange the status information of link, as the plan of routing extension to exchange the information of various link type, bandwidth, link protection type etc. And also, we describe the definition of new protocol, so called, LMP that is a signaling protocol for solving complex problem which manages hundreds and thousands of links between two nodes. And we will examine and analyze the plan of signaling protocol extension to apply signaling protocol RSVP-TE(Resource Reservation Protocol) for traffic engineering in MPLS to network, and the message objects and formats associated with modified RSVP.

• Journal of KSNVE
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• v.7 no.3
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• pp.427-437
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• 1997

Mean active intensity based active control for the cancellation of radiated noise out of the duct exit is studied. The active intensity control strategy is drerived based on the relation of the exterior sound field out of the duct termination and interior sound field of the duct. One of the characteristics of this control strategy is that the control performance can be maintained regardless of the sensor loction, compared with the conventional local pressure control methods at either interior downstream or exterior field positions. It is also suggested that the digital filtering for the active intensity control can be achieved by time-domain filtered-x LMP (Lest-Mean-Product) adaptive algorithm. Experiments for an open-ended duct are performed to compare the active intensity control performance with conventional pressure control one. Active control experiment of local sound pressure is conducted by widely used filtered-x LMS adaptive Algorithm and active intensity control implementaion uses the derived filter d-x LMP algorithm. It is shown that the exterior sound fileds was much better observable by sensing of the active intensity than by just sound pressure. It is also demonstrated that the global control performance of external field by acoustic intensity is superior to the conventional sound pressure control performance.

• Journal of Ocean Engineering and Technology
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• v.15 no.2
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• pp.46-52
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• 2001

In this paper, the real-time prediction of high temperature creep life was carried out for the friction welded joints of dissimilar heat resisting steels (SUH3-SUH35). various life prediction method such as LMP (Larson_miller Parameter) and ISM (initial strain method) were applied. The creep behaviors of those steels and the welds under static load were examined by ISM combined with LMP at 500, 600 and $700^{\circ}C$, and the relationship between these two methods was investigated. A real-time creep lie (tr, hr) prediction equation by initial strain (${\varepsilon}_0$, %) under any creep stress ($\sigma$, MPa) at any high temperature (T, K) was developed as follows: $t_r={\alpha}{\varepsilon}_0^{\beta}{\sigma}^{-1}$ where, ${\phi}=16: {\alpha}=10^{51.412-0.104T+5.375{\times}10^5T^2}$, $ {\beta}=-83.989+0.180T-9.957{\times}10^{-5}T^2,{\phi}=20:$ ${\alpha}=10^{69.910-0.146T+7.744{\times}10^{-5}T^2$, ${\beta}=-51.442+0.105T-5.595{\times}10^{-5}T^2$ for SUH3-SUH35 friction weld of =16mm and 20mm, respectively.

• Korean Journal of Agricultural Science
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• v.48 no.4
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• pp.655-668
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• 2021

The present study was conducted to investigate the effects of the supplementation of Achyranthes japonica extract (AJE) on overall performance levels with different nutrition concentrations in the diets of finishing pigs. Here, 140 finishing pigs with initial body weights of 50.18 ± 2.37 kg were used in a ten-week trial involving a 2 × 2 factorial design in two phases with the following factors: diet types with different nutrition concentrations (Positive control [PC] vs. Negative control [NC]) and 0 or 0.05% AJE supplementation to NC and PC diets. The PC diet was a basal diet, whereas the NC diet consisted of a 5% and 7% crude protein (CP) reduced basal diet during phase 1 (1 - 35 days) and phase 2 (36 - 70 days), respectively. A significant effect (p < 0.05) on the gain-to-feed ratio (G : F) was observed with interaction effects between the diets and AJE supplementation. However, during phase 1 and in all periods, the pigs fed the PC and NC diets with average daily feed intake (ADFI) tended to decrease compared to those fed diets with AJE. A significant effect (p = 0.0380) of E. coli was observed in pigs fed the PC and NC diets compared to AJE supplementation in phase 2 of the experiment. The backfat thickness (BFT) tended to decrease and the lean meat percentage (LMP) was significantly improved in phase 2 for pigs fed the PC and NC diets. In summary, BFT and LMP showed beneficial effects and fecal microbiota of E. coli counts were positively affected when pigs were fed diets with different nutrient concentrations.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.57 no.7
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• pp.1157-1166
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• 2008

This paper presents a new approach for the evaluation of location marginal prices (LMPs) considering demand-side bidding (DSB) in a competitive electricity market. The stabilization of the electric power supply and demand balance is one of the major important activities in electric power industry. In this paper, we present an analytical method for calculation of LMPs considering DSB, which has opportunity to compete with generating units, as England & Wales Pool's DSB scheme[1]. Also, we propose a new approach that LMP considering DSB is divided into three components. The proposed approach can be used for the evaluation of demand-side bidding into the electricity market and the assessment of the influence of DSB on total production costs and LMPs as well as three components.

• Journal of the Korean Society of Food Science and Nutrition
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• v.12 no.1
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• pp.7-11
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• 1983

The purpose of this study was to prepare low methoxyl pectins (LMPs) by pectinesterase (PE) using waste tangerine peels and to compare the chemical, physical and gelling properties of the prepared pectins with the commercial LMP. The LMPs were prepared by treating albedo with 0.25M $Na_2CO_3$ to activate the PE. PE was then inactivated by heating. The sample was centrifuged and the supernatant was collected. LMP was obtained as precipitate by adding alcohol to the collected supernatant. The amounts of extractible pectins in albedo were 12.71~12.98% on a dry weight basis. Methoxyl contents of LMPs prepared by treating albedo with PE at pH 7.5 for 10min, at pH 8.5 for 10min, 20min and 30min were 5.12%, 4.27%, 3.08%, 1.85% respectively, demonstrating that the methoxyl contents of the preparations decreased as the degree of treatment albedo with PE increased. The acetyl contents of the preparations ranged from 0.09% to 0.12%, the values of which do not interfere with gel formation. The anhydrouronic acid contents of the prepared pectins were in the range of 94.2%~94.8%. The values of viscosity and molecular weights of the prepared LMPs tent to decrease as the degree of PE action on albedo increased. The textural value of the prepared LMP gels determined by Consistometer, Ridgelimeter and Instron denonstrated that the excess treatment of albedo with PE significantly impaired the gelling properties of the preparations.

• The Plant Pathology Journal
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• v.27 no.3
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• pp.280-284
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• 2011

Fusarium wilt of banana caused by Fusarium oxysporum f. sp. cubense is widespread in Indonesia. However, the distribution of tropical race 4 strains has not been well studied. Thirty nine isolates of F. oxysporum f. sp. cubense were collected from Java and 7 isolates were from Sumatera, Bangka, and Kalimantan. All isolates produced volatile odor when grown on steamed rice. These isolates were further tested for their vegetative compatibility with nitM testers of 20 reported vegetative compatibility groups representing strains that belong to race 1, 2, and 4. Three isolates formed heterokaryons with nitM testers belong to race 1, 11 isolates with race 4, and the rest did not form heterokaryons with all nitM testers used. F. oxysporum f. sp. cubense tropical race 4 specific primer pair was used to amplify a 1400 bp fragment of tropical race 4 DNA. Seven isolates (Bnt2, Mln1, Srg1, Bgl3, Bgl6, Lmp1, and Kjg1) produced the 1400 bp amplification product were therefore tropical race 4.